Restriction map for
pFN21AA0580
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AcyI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstZ17I Bsu36I CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoT22I FauI FseI FspI HaeII
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 2814 GTCTAC GT^[AC][GT]AC
AccII 451 CGCG CG^CG
AclI 4649 AACGTT AA^CGTT
AflII 3177 CTTAAG C^TTAAG
AflIII 2934 ACGTGT A^C[GA][CT]GT
AgeI 4373 ACCGGT A^CCGGT
ApaI 2896 GGGCCC GGGCC^C
AvaI 1193 CTCGAG C^[CT]CG[GA]G
BalI 4624 TGGCCA TGG^CCA
BcgI 4191 GCAGGTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4727 CCCGG CC^[GC]GG
BfrI 3177 CTTAAG C^TTAAG
BglI 4949 GCCATAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3366 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3366 GCTCAGC GC^T[ACGT]AGC
BsmFI 3828 GGGAC GGGAC(10/14)
BsoBI 1193 CTCGAG C^[CT]CG[GA]G
BsrDI 2243 GCAATG GCAATG(2/0)
BsrFI 4373 ACCGGT [GA]^CCGG[CT]
BstEII 163 GGTCACC G^GT[ACGT]ACC
BstPI 163 GGTCACC G^GT[ACGT]ACC
BstUI 451 CGCG CG^CG
CelII 3366 GCTCAGC GC^T[ACGT]AGC
Cfr10I 4373 ACCGGT [GA]^CCGG[CT]
DraII 3860 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 4624 TGGCCA [CT]^GGCC[GA]
EcoNI 4102 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3860 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 163 GGTCACC G^GT[ACGT]ACC
EcoRI 978 GAATTC G^AATTC
HgaI 4158 GCGTC GACGC(5/10)
HindIII 3513 AAGCTT A^AGCTT
MfeI 3317 CAATTG C^AATTG
MluNI 4624 TGGCCA TGG^CCA
MscI 4624 TGGCCA TGG^CCA
MunI 3317 CAATTG C^AATTG
MvnI 451 CGCG CG^CG
NciI 4727 CCCGG CC^[GC]GG
NdeI 2332 CATATG CA^TATG
NspI 1493 GCATGT [GA]CATG^[CT]
PaeR7I 1193 CTCGAG C^TCGAG
PinAI 4373 ACCGGT A^CCGGT
PpuMI 3860 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 4649 AACGTT AA^CGTT
SacI 3610 GAGCTC GAGCT^C
SapI 4147 GAAGAGC GCTCTTC(1/4)
SexAI 4235 ACCTGGT A^CC[AT]GGT
SfiI 4948 GGCCATAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3835 ACTAGT A^CTAGT
StuI 4956 AGGCCT AGG^CCT
SwaI 2885 ATTTAAAT ATTT^AAAT
XbaI 2475 TCTAGA T^CTAGA
XcmI 2116 CCAGATCCTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1193 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 3610 GAGCTC G[AT]GC[AT]^C
4849 GAGCAC
BanI 2830 GGCACC G^G[CT][GA]CC
4544 GGCACC
BanII 2896 GGGCCC G[GA]GC[CT]^C
3610 GAGCTC
BglII 2182 AGATCT A^GATCT
2915 AGATCT
BsaI 1006 GAGACC GGTCTC(1/5)
2528 GAGACC
BsiHKAI 3610 GAGCTC G[AT]GC[AT]^C
4849 GAGCAC
BspMI 3331 GCAGGT ACCTGC(4/8)
4191 GCAGGT
BstAPI 1096 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2574 GCAAAGTCTGC
BstBI 1566 TTCGAA TT^CGAA
4868 TTCGAA
BstXI 81 CCATGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4501 CCAACAAGCTGG
DraI 2886 TTTAAA TTT^AAA
3517 TTTAAA
EcoRV 1775 GATATC GAT^ATC
4038 GATATC
NcoI 3276 CCATGG C^CATGG
4829 CCATGG
NheI 2230 GCTAGC G^CTAGC
2556 GCTAGC
NspV 1566 TTCGAA TT^CGAA
4868 TTCGAA
PvuII 3399 CAGCTG CAG^CTG
4631 CAGCTG
SfuI 1566 TTCGAA TT^CGAA
4868 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AvaII BbsI BbvI BciVI BclI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EarI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PshBI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI