Restriction map for
pFN21AA0580
(Linear) (Six-base) MAPSORT of: KIAA0580.seq Check: 4116 from: 1 to: 5112
KIAA0580.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4182 5112
Size: 4182 930
AccI GT'mk_AC
Cuts at: 0 2815 5112
Size: 2815 2297
AclI AA'CG_TT
Cuts at: 0 4650 5112
Size: 4650 462
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2714 4943 5112
Size: 2714 2229 169
Fragments arranged by size:
2714 2229 169
AflII C'TTAA_G
Cuts at: 0 3177 5112
Size: 3177 1935
AflIII A'CryG_T
Cuts at: 0 2934 5112
Size: 2934 2178
AgeI A'CCGG_T
Cuts at: 0 4373 5112
Size: 4373 739
AleI CACnn'nnGTG
Cuts at: 0 4280 5112
Size: 4280 832
AlwNI CAG_nnn'CTG
Cuts at: 0 640 1102 2096 2122 3037 4921 5112
Size: 640 462 994 26 915 1884 191
Fragments arranged by size:
1884 994 915 640 462 191 26
ApaI G_GGCC'C
Cuts at: 0 2900 5112
Size: 2900 2212
ApoI r'AATT_y
Cuts at: 0 483 681 963 978 1032 1060 1284 1516
Size: 483 198 282 15 54 28 224 232
Cuts at: 1516 1624 1805 2392 2590 2608 5112
Size: 108 181 587 198 18 2504
Fragments arranged by size:
2504 587 483 282 232 224 198 198
181 108 54 28 18 15
AseI AT'TA_AT
Cuts at: 0 53 2837 3869 5112
Size: 53 2784 1032 1243
Fragments arranged by size:
2784 1243 1032 53
AvaI C'yCGr_G
Cuts at: 0 1193 5112
Size: 1193 3919
BanI G'GyrC_C
Cuts at: 0 2830 4544 5112
Size: 2830 1714 568
Fragments arranged by size:
2830 1714 568
BanII G_rGCy'C
Cuts at: 0 2900 3614 5112
Size: 2900 714 1498
Fragments arranged by size:
2900 1498 714
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 559 2657 3189 4981 5112
Size: 389 170 2098 532 1792 131
Fragments arranged by size:
2098 1792 532 389 170 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4180 4214 5112
Size: 4180 34 898
Fragments arranged by size:
4180 898 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 170 425 1181 5112
Size: 170 255 756 3931
Fragments arranged by size:
3931 756 255 170
BclI T'GATC_A
Cuts at: 0 294 602 5013 5112
Size: 294 308 4411 99
Fragments arranged by size:
4411 308 294 99
BglI GCCn_nnn'nGGC
Cuts at: 0 4955 5112
Size: 4955 157
BglII A'GATC_T
Cuts at: 0 2182 2915 5112
Size: 2182 733 2197
Fragments arranged by size:
2197 2182 733
BlpI GC'TnA_GC
Cuts at: 0 3367 5112
Size: 3367 1745
Bme1580I G_kGCm'C
Cuts at: 0 2900 5112
Size: 2900 2212
BmrI ACTGGGnnnn_n'
Cuts at: 0 2136 2664 2903 4306 5112
Size: 2136 528 239 1403 806
Fragments arranged by size:
2136 1403 806 528 239
BmtI G_CTAG'C
Cuts at: 0 2234 2560 5112
Size: 2234 326 2552
Fragments arranged by size:
2552 2234 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 5112
Size: 1099 4013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3508 3995 4207 4867 4927 5112
Size: 3508 487 212 660 60 185
Fragments arranged by size:
3508 660 487 212 185 60
Bpu10I CC'TnA_GC
Cuts at: 0 1528 5112
Size: 1528 3584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1466 1730 2811 2948 5112
Size: 1466 264 1081 137 2164
Fragments arranged by size:
2164 1466 1081 264 137
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 2522 5112
Size: 1000 1522 2590
Fragments arranged by size:
2590 1522 1000
BsaWI w'CCGG_w
Cuts at: 0 2466 2524 4286 4373 5112
Size: 2466 58 1762 87 739
Fragments arranged by size:
2466 1762 739 87 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2495 2519 2525 2549 2911 2941 5112
Size: 2495 24 6 24 362 30 2171
Fragments arranged by size:
2495 2171 362 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 431 988 1469 1693 5112
Size: 431 557 481 224 3419
Fragments arranged by size:
3419 557 481 431 224
BseYI C'CCAG_C
Cuts at: 0 2569 4508 4604 5112
Size: 2569 1939 96 508
Fragments arranged by size:
2569 1939 508 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 2041 2120 2189 5112
Size: 134 1907 79 69 2923
Fragments arranged by size:
2923 1907 134 79 69
BsiHKAI G_wGCw'C
Cuts at: 0 3614 4853 5112
Size: 3614 1239 259
Fragments arranged by size:
3614 1239 259
BsmI GAATG_Cn'
Cuts at: 0 649 1264 3176 3783 4719 5112
Size: 649 615 1912 607 936 393
Fragments arranged by size:
1912 936 649 615 607 393
Bsp1286I G_dGCh'C
Cuts at: 0 2900 3614 4853 5112
Size: 2900 714 1239 259
Fragments arranged by size:
2900 1239 714 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3322 4182 5112
Size: 3322 860 930
Fragments arranged by size:
3322 930 860
BsrDI GCAATG_nn'
Cuts at: 0 2250 5112
Size: 2250 2862
BsrFI r'CCGG_y
Cuts at: 0 4373 5112
Size: 4373 739
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1102 2580 5112
Size: 1102 1478 2532
Fragments arranged by size:
2532 1478 1102
BstBI TT'CG_AA
Cuts at: 0 1567 4869 5112
Size: 1567 3302 243
Fragments arranged by size:
3302 1567 243
BstEII G'GTnAC_C
Cuts at: 0 163 5112
Size: 163 4949
BstXI CCAn_nnnn'nTGG
Cuts at: 0 88 4508 5112
Size: 88 4420 604
Fragments arranged by size:
4420 604 88
BstYI r'GATC_y
Cuts at: 0 2118 2182 2915 3626 5112
Size: 2118 64 733 711 1486
Fragments arranged by size:
2118 1486 733 711 64
BtgI C'CryG_G
Cuts at: 0 3276 4799 4829 5112
Size: 3276 1523 30 283
Fragments arranged by size:
3276 1523 283 30
BtsI GCAGTG_nn'
Cuts at: 0 461 1223 3595 4275 4287 4839 5112
Size: 461 762 2372 680 12 552 273
Fragments arranged by size:
2372 762 680 552 461 273 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 338 373 5112
Size: 338 35 4739
Fragments arranged by size:
4739 338 35
DraI TTT'AAA
Cuts at: 0 2888 3519 5112
Size: 2888 631 1593
Fragments arranged by size:
2888 1593 631
EaeI y'GGCC_r
Cuts at: 0 4624 5112
Size: 4624 488
EarI CTCTTCn'nnn_
Cuts at: 0 1021 1670 2733 2744 3067 3698 4142 4169
Size: 1021 649 1063 11 323 631 444 27
Cuts at: 4169 4240 4417 5112
Size: 71 177 695
Fragments arranged by size:
1063 1021 695 649 631 444 323 177
71 27 11
EcoICRI GAG'CTC
Cuts at: 0 3612 5112
Size: 3612 1500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2714 3508 3995 4207 4867 4927 4943 5112
Size: 2714 794 487 212 660 60 16 169
Fragments arranged by size:
2714 794 660 487 212 169 60 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4106 5112
Size: 4106 1006
EcoO109I rG'GnC_Cy
Cuts at: 0 3861 5112
Size: 3861 1251
EcoRI G'AATT_C
Cuts at: 0 978 5112
Size: 978 4134
EcoRV GAT'ATC
Cuts at: 0 1777 4040 5112
Size: 1777 2263 1072
Fragments arranged by size:
2263 1777 1072
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4269 4948 5112
Size: 4269 679 164
Fragments arranged by size:
4269 679 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 421 453 546 578 967 999 1459 1491
Size: 421 32 93 32 389 32 460 32
Cuts at: 1491 2116 2148 2495 2527 4434 4466 4780 4812
Size: 625 32 347 32 1907 32 314 32
Cuts at: 4812 5112
Size: 300
Fragments arranged by size:
1907 625 460 421 389 347 314 300
93 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 884 3058 3705 5112
Size: 884 2174 647 1407
Fragments arranged by size:
2174 1407 884 647
HindIII A'AGCT_T
Cuts at: 0 3513 5112
Size: 3513 1599
MfeI C'AATT_G
Cuts at: 0 3317 5112
Size: 3317 1795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 2111 3272 4525 5112
Size: 338 1773 1161 1253 587
Fragments arranged by size:
1773 1253 1161 587 338
MscI TGG'CCA
Cuts at: 0 4626 5112
Size: 4626 486
MslI CAynn'nnrTG
Cuts at: 0 80 3391 3476 3776 4280 4613 5112
Size: 80 3311 85 300 504 333 499
Fragments arranged by size:
3311 504 499 333 300 85 80
MspA1I CmG'CkG
Cuts at: 0 3401 4261 4633 5112
Size: 3401 860 372 479
Fragments arranged by size:
3401 860 479 372
NcoI C'CATG_G
Cuts at: 0 3276 4829 5112
Size: 3276 1553 283
Fragments arranged by size:
3276 1553 283
NdeI CA'TA_TG
Cuts at: 0 2333 5112
Size: 2333 2779
NheI G'CTAG_C
Cuts at: 0 2230 2556 5112
Size: 2230 326 2556
Fragments arranged by size:
2556 2230 326
NspI r_CATG'y
Cuts at: 0 1497 5112
Size: 1497 3615
PasI CC'CwG_GG
Cuts at: 0 1071 5112
Size: 1071 4041
PflMI CCAn_nnn'nTGG
Cuts at: 0 3084 3789 4633 4845 5112
Size: 3084 705 844 212 267
Fragments arranged by size:
3084 844 705 267 212
PfoI T'CCnGG_A
Cuts at: 0 3485 5112
Size: 3485 1627
PpuMI rG'GwC_Cy
Cuts at: 0 3861 5112
Size: 3861 1251
PsiI TTA'TAA
Cuts at: 0 4030 5112
Size: 4030 1082
PspOMI G'GGCC_C
Cuts at: 0 2896 5112
Size: 2896 2216
PstI C_TGCA'G
Cuts at: 0 140 468 4282 5112
Size: 140 328 3814 830
Fragments arranged by size:
3814 830 328 140
PvuII CAG'CTG
Cuts at: 0 3401 4633 5112
Size: 3401 1232 479
Fragments arranged by size:
3401 1232 479
SacI G_AGCT'C
Cuts at: 0 3614 5112
Size: 3614 1498
SapI GCTCTTCn'nnn_
Cuts at: 0 4142 5112
Size: 4142 970
SexAI A'CCwGG_T
Cuts at: 0 4235 5112
Size: 4235 877
SfcI C'TryA_G
Cuts at: 0 136 158 464 1959 2456 4020 4278 5112
Size: 136 22 306 1495 497 1564 258 834
Fragments arranged by size:
1564 1495 834 497 306 258 136 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4955 5112
Size: 4955 157
SmlI C'TyrA_G
Cuts at: 0 1193 1481 1745 2790 2963 3177 5112
Size: 1193 288 264 1045 173 214 1935
Fragments arranged by size:
1935 1193 1045 288 264 214 173
SpeI A'CTAG_T
Cuts at: 0 3835 5112
Size: 3835 1277
SspI AAT'ATT
Cuts at: 0 525 3887 4095 4737 5112
Size: 525 3362 208 642 375
Fragments arranged by size:
3362 642 525 375 208
StuI AGG'CCT
Cuts at: 0 4958 5112
Size: 4958 154
StyI C'CwwG_G
Cuts at: 0 2204 3276 3794 4721 4829 5112
Size: 2204 1072 518 927 108 283
Fragments arranged by size:
2204 1072 927 518 283 108
SwaI ATTT'AAAT
Cuts at: 0 2888 5112
Size: 2888 2224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1500 1937 3973 5112
Size: 1500 437 2036 1139
Fragments arranged by size:
2036 1500 1139 437
TatI w'GTAC_w
Cuts at: 0 4182 4291 5112
Size: 4182 109 821
Fragments arranged by size:
4182 821 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1817 1849 2519 2551 5112
Size: 1817 32 670 32 2561
Fragments arranged by size:
2561 1817 670 32 32
XbaI T'CTAG_A
Cuts at: 0 2475 5112
Size: 2475 2637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2123 5112
Size: 2123 2989
XhoI C'TCGA_G
Cuts at: 0 1193 5112
Size: 1193 3919
XmnI GAAnn'nnTTC
Cuts at: 0 681 1262 1284 4249 4650 5112
Size: 681 581 22 2965 401 462
Fragments arranged by size:
2965 681 581 462 401 22
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApoI AseI AvaI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI
CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NdeI NheI NspI PasI
PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SacI
SapI SexAI SfcI SfiI SmlI SpeI SspI StuI
StyI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AhdI AlfI AloI ApaLI AscI
AsiSI AvrII BaeI BamHI BbeI BbvCI BfrBI BmgBI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstZ17I Bsu36I BtgZI ClaI
DraIII DrdI EagI EciI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NgoMIV NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
Tth111I XmaI ZraI