(Circular) (Six-base) MAPSORT of: pFN21AA0419.seq Check: 8568 from: 1 to: 7678 pFN21AA0419 7678 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5488 278 Size: 53 83 186 936 3952 2468 Fragments arranged by size: 3952 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4441 4897 5021 1937 Size: 2504 456 124 4594 Fragments arranged by size: 4594 2504 456 124 Acc65I G'GTAC_C Cuts at: 4999 4999 Size: 7678 AclI AA'CG_TT Cuts at: 1563 5805 6178 1563 Size: 4242 373 3063 Fragments arranged by size: 4242 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 2672 3366 3381 4484 4553 4835 5741 Size: 930 110 694 15 1103 69 282 906 Cuts at: 5741 7125 7393 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1103 930 906 694 282 268 110 69 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7481 AflIII A'CryG_T Cuts at: 1203 6577 7554 1203 Size: 5374 977 1327 Fragments arranged by size: 5374 1327 977 AgeI A'CCGG_T Cuts at: 6509 6509 Size: 7678 AhdI GACnn_n'nnGTC Cuts at: 6407 6407 Size: 7678 AleI CACnn'nnGTG Cuts at: 1166 3263 1166 Size: 2097 5581 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2960 1184 Size: 1776 5902 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2980 3012 2980 Size: 32 7646 AlwNI CAG_nnn'CTG Cuts at: 2695 3495 5421 6993 2695 Size: 800 1926 1572 3380 Fragments arranged by size: 3380 1926 1572 800 ApaLI G'TGCA_C Cuts at: 5735 6891 5735 Size: 1156 6522 ApoI r'AATT_y Cuts at: 1493 4216 4397 4499 4811 4987 5213 5365 7474 Size: 2723 181 102 312 176 226 152 2109 Cuts at: 7474 1493 Size: 1697 Fragments arranged by size: 2723 2109 1697 312 226 181 176 152 102 AseI AT'TA_AT Cuts at: 160 1032 6232 160 Size: 872 5200 1606 Fragments arranged by size: 5200 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7678 AvaI C'yCGr_G Cuts at: 1870 1943 5003 1870 Size: 73 3060 4545 Fragments arranged by size: 4545 3060 73 BamHI G'GATC_C Cuts at: 5008 5375 5008 Size: 367 7311 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4999 6454 618 Size: 325 217 627 3212 1455 1842 Fragments arranged by size: 3212 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4997 1391 Size: 3606 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2678 3142 4966 928 Size: 971 779 464 1824 3640 Fragments arranged by size: 3640 1824 971 779 464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5869 5903 1903 Size: 34 3932 34 3678 Fragments arranged by size: 3932 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5570 6780 5570 Size: 1210 6468 BclI T'GATC_A Cuts at: 1548 2305 1548 Size: 757 6921 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6289 136 Size: 107 122 71 631 605 4617 1525 Fragments arranged by size: 4617 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7673 7673 Size: 7678 BlpI GC'TnA_GC Cuts at: 4020 5095 4020 Size: 1075 6603 Bme1580I G_kGCm'C Cuts at: 1790 3270 5739 6895 1790 Size: 1480 2469 1156 2573 Fragments arranged by size: 2573 2469 1480 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7678 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5409 6367 442 Size: 416 53 83 399 24 3992 958 1753 Fragments arranged by size: 3992 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2961 1056 Size: 1905 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6338 1239 Size: 5099 2579 Bpu10I CC'TnA_GC Cuts at: 4697 4697 Size: 7678 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2910 3563 4176 4353 4539 5803 6668 6966 2910 Size: 653 613 177 186 1264 865 298 3622 Fragments arranged by size: 3622 1264 865 653 613 298 186 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4371 6341 882 Size: 617 2872 1970 2219 Fragments arranged by size: 2872 2219 1970 617 BsaAI yAC'GTr Cuts at: 493 1204 5469 493 Size: 711 4265 2702 Fragments arranged by size: 4265 2702 711 BsaBI GATnn'nnATC Cuts at: 2304 7672 2304 Size: 5368 2310 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5485 5867 6502 275 Size: 53 83 186 936 3952 382 635 1451 Fragments arranged by size: 3952 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6111 6509 6783 6930 7341 6111 Size: 398 274 147 411 6448 Fragments arranged by size: 6448 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4061 4091 1178 Size: 30 165 30 2658 30 4765 Fragments arranged by size: 4765 2658 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 3944 1186 Size: 838 1920 4920 Fragments arranged by size: 4920 1920 838 BseYI C'CCAG_C Cuts at: 2432 3996 6881 7377 2432 Size: 1564 2885 496 2733 Fragments arranged by size: 2885 2733 1564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5128 5889 6917 1540 Size: 275 186 3127 761 1028 2301 Fragments arranged by size: 3127 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5739 5824 6895 5739 Size: 85 1071 6522 Fragments arranged by size: 6522 1071 85 BsmI GAATG_Cn' Cuts at: 4803 4876 5189 5282 4803 Size: 73 313 93 7199 Fragments arranged by size: 7199 313 93 73 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3270 4997 5739 5824 6895 1391 Size: 399 1480 1727 742 85 1071 2174 Fragments arranged by size: 2174 1727 1480 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7341 7341 Size: 7678 BspHI T'CATG_A Cuts at: 4615 4666 5567 7297 4615 Size: 51 901 1730 4996 Fragments arranged by size: 4996 1730 901 51 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2206 5033 844 Size: 1362 2827 3489 Fragments arranged by size: 3489 2827 1362 BsrBI CCG'CTC Cuts at: 1394 1823 5565 1394 Size: 429 3742 3507 Fragments arranged by size: 3742 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2528 3231 4101 6173 6347 65 Size: 1183 1280 703 870 2072 174 1396 Fragments arranged by size: 2072 1396 1280 1183 870 703 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6322 6509 1700 Size: 110 4512 187 2869 Fragments arranged by size: 4512 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7678 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7678 BssSI C'ACGA_G Cuts at: 2244 4725 5738 6750 2244 Size: 2481 1013 1012 3172 Fragments arranged by size: 3172 2481 1013 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2291 3850 794 Size: 1497 1559 4622 Fragments arranged by size: 4622 1559 1497 BstBI TT'CG_AA Cuts at: 2966 6482 2966 Size: 3516 4162 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7678 BstYI r'GATC_y Cuts at: 1972 2638 2668 3875 5008 5375 5760 5777 7229 Size: 666 30 1207 1133 367 385 17 1452 Cuts at: 7229 7315 7327 7673 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1207 1133 666 385 367 346 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3870 3870 Size: 7678 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6187 Fragments arranged by size: 6187 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3518 487 Size: 683 56 2292 4647 Fragments arranged by size: 4647 2292 683 56 BtsI GCAGTG_nn' Cuts at: 2141 4140 4422 5194 5988 6008 2141 Size: 1999 282 772 794 20 3811 Fragments arranged by size: 3811 1999 794 772 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7678 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7643 DraI TTT'AAA Cuts at: 3028 4982 3028 Size: 1954 5724 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6685 809 Size: 507 5369 1802 Fragments arranged by size: 5369 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 4935 5125 Size: 54 1425 50 275 18 1162 1943 190 Cuts at: 5125 6014 7643 8 Size: 889 1629 43 Fragments arranged by size: 1943 1629 1425 1162 889 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5125 1537 Size: 275 3313 4090 Fragments arranged by size: 4090 3313 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2581 3968 4056 4342 5608 1353 Size: 1228 1387 88 286 1266 3423 Fragments arranged by size: 3423 1387 1266 1228 286 88 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6265 6639 6785 387 Size: 1064 138 4676 374 146 1280 Fragments arranged by size: 4676 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 2672 3366 3381 4484 4553 4835 Size: 393 930 110 694 15 1103 69 282 Cuts at: 4835 5741 6338 7125 7393 1239 Size: 906 597 787 268 1524 Fragments arranged by size: 1524 1103 930 906 787 694 597 393 282 268 110 69 15 EcoRI G'AATT_C Cuts at: 4499 4987 5365 4499 Size: 488 378 6812 Fragments arranged by size: 6812 488 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2615 4448 2615 Size: 1833 5845 FspI TGC'GCA Cuts at: 6184 6184 Size: 7678 HaeII r_GCGC'y Cuts at: 1678 6825 1678 Size: 5147 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 6319 6351 6393 Size: 32 135 32 543 32 4172 32 42 Cuts at: 6393 6425 1373 Size: 32 2626 Fragments arranged by size: 4172 2626 543 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2221 2844 3456 3889 5022 5268 Size: 988 281 283 623 612 433 1133 246 Cuts at: 5268 669 Size: 3079 Fragments arranged by size: 3079 1133 988 623 612 433 283 281 246 HindIII A'AGCT_T Cuts at: 748 2232 2417 2559 748 Size: 1484 185 142 5867 Fragments arranged by size: 5867 1484 185 142 HpaI GTT'AAC Cuts at: 2221 3456 3889 5268 2221 Size: 1235 433 1379 4631 Fragments arranged by size: 4631 1379 1235 433 KpnI G_GTAC'C Cuts at: 5003 5003 Size: 7678 MfeI C'AATT_G Cuts at: 5277 5277 Size: 7678 MluI A'CGCG_T Cuts at: 7554 7554 Size: 7678 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4098 6792 6976 1305 Size: 2793 2694 184 2007 Fragments arranged by size: 2793 2694 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2994 10 Size: 54 1425 343 1162 4694 Fragments arranged by size: 4694 1425 1162 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2942 3157 3263 3704 5636 5995 6154 Size: 648 1776 215 106 441 1932 359 159 Cuts at: 6154 518 Size: 2042 Fragments arranged by size: 2042 1932 1776 648 441 359 215 159 106 MspA1I CmG'CkG Cuts at: 1606 4103 4643 4742 5094 5771 6919 7164 1606 Size: 2497 540 99 352 677 1148 245 2120 Fragments arranged by size: 2497 2120 1148 677 540 352 245 99 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6187 Fragments arranged by size: 6187 939 552 NdeI CA'TA_TG Cuts at: 387 3085 3526 387 Size: 2698 441 4539 Fragments arranged by size: 4539 2698 441 NheI G'CTAG_C Cuts at: 1052 2957 1052 Size: 1905 5773 NotI GC'GGCC_GC Cuts at: 5125 5125 Size: 7678 NspI r_CATG'y Cuts at: 1132 1229 5036 6581 1132 Size: 97 3807 1545 2229 Fragments arranged by size: 3807 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7678 PciI A'CATG_T Cuts at: 6577 6577 Size: 7678 PflMI CCAn_nnn'nTGG Cuts at: 1108 3560 1108 Size: 2452 5226 PfoI T'CCnGG_A Cuts at: 2250 3143 2250 Size: 893 6785 PmeI GTTT'AAAC Cuts at: 4982 4982 Size: 7678 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5747 5779 7260 7292 5747 Size: 32 1481 32 6133 Fragments arranged by size: 6133 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2246 1613 Size: 633 7045 PsiI TTA'TAA Cuts at: 3173 5248 3173 Size: 2075 5603 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2006 2038 2006 Size: 32 7646 PstI C_TGCA'G Cuts at: 830 2413 3611 4417 5030 830 Size: 1583 1198 806 613 3478 Fragments arranged by size: 3478 1583 1198 806 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7678 PvuII CAG'CTG Cuts at: 4643 4742 4643 Size: 99 7579 SalI G'TCGA_C Cuts at: 1936 5020 1936 Size: 3084 4594 SapI GCTCTTCn'nnn_ Cuts at: 4056 4342 4056 Size: 286 7392 SbfI CC_TGCA'GG Cuts at: 5030 5030 Size: 7678 ScaI AGT'ACT Cuts at: 5926 7638 5926 Size: 1712 5966 SfcI C'TryA_G Cuts at: 826 1045 2409 3607 4413 5026 6161 6842 7033 Size: 219 1364 1198 806 613 1135 681 191 Cuts at: 7033 826 Size: 1471 Fragments arranged by size: 1471 1364 1198 1135 806 681 613 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7678 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7678 SmaI CCC'GGG Cuts at: 1872 5005 1872 Size: 3133 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2925 3578 4191 4368 4554 5782 Size: 197 926 982 653 613 177 186 1228 Cuts at: 5782 6683 6945 820 Size: 901 262 1553 Fragments arranged by size: 1553 1228 982 926 901 653 613 262 197 186 177 SnaBI TAC'GTA Cuts at: 493 5469 493 Size: 4976 2702 SpeI A'CTAG_T Cuts at: 152 3911 4307 152 Size: 3759 396 3523 Fragments arranged by size: 3759 3523 396 SphI G_CATG'C Cuts at: 1132 5036 1132 Size: 3904 3774 SspI AAT'ATT Cuts at: 5 52 2539 5602 5 Size: 47 2487 3063 2081 Fragments arranged by size: 3063 2487 2081 47 StuI AGG'CCT Cuts at: 1517 4339 1517 Size: 2822 4856 StyI C'CwwG_G Cuts at: 513 1065 2004 2911 4861 5117 513 Size: 552 939 907 1950 256 3074 Fragments arranged by size: 3074 1950 939 907 552 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5705 5722 5875 6060 6505 325 Size: 1255 4125 17 153 185 445 1498 Fragments arranged by size: 4125 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5924 7636 96 Size: 274 80 33 51 1443 3947 1712 138 Fragments arranged by size: 3947 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7638 7670 7638 Size: 32 7646 Tth111I GACn'n_nGTC Cuts at: 1316 2742 1316 Size: 1426 6252 XbaI T'CTAG_A Cuts at: 2735 3007 5014 2735 Size: 272 2007 5399 Fragments arranged by size: 5399 2007 272 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4290 4290 Size: 7678 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7678 XmaI C'CCGG_G Cuts at: 1870 5003 1870 Size: 3133 4545 XmnI GAAnn'nnTTC Cuts at: 2562 3139 3913 5807 2562 Size: 577 774 1894 4433 Fragments arranged by size: 4433 1894 774 577 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5486 276 Size: 53 83 186 936 3952 2468 Fragments arranged by size: 3952 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI