Restriction map for
pFN21AA0419
(Linear) (Six-base) MAPSORT of: KIAA0419.seq Check: 203 from: 1 to: 2973
KIAA0419.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 2436 2892 2973
Size: 2436 456 81
Fragments arranged by size:
2436 456 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 667 1361 1376 2479 2548 2830 2973
Size: 557 110 694 15 1103 69 282 143
Fragments arranged by size:
1103 694 557 282 143 110 69 15
AleI CACnn'nnGTG
Cuts at: 0 1258 2973
Size: 1258 1715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 955 2973
Size: 955 2018
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 975 1007 2973
Size: 975 32 1966
Fragments arranged by size:
1966 975 32
AlwNI CAG_nnn'CTG
Cuts at: 0 690 1490 2973
Size: 690 800 1483
Fragments arranged by size:
1483 800 690
ApoI r'AATT_y
Cuts at: 0 2211 2392 2494 2806 2973
Size: 2211 181 102 312 167
Fragments arranged by size:
2211 312 181 167 102
BbsI GAAGACnn'nnnn_
Cuts at: 0 673 1137 2961 2973
Size: 673 464 1824 12
Fragments arranged by size:
1824 673 464 12
BclI T'GATC_A
Cuts at: 0 300 2973
Size: 300 2673
BlpI GC'TnA_GC
Cuts at: 0 2015 2973
Size: 2015 958
Bme1580I G_kGCm'C
Cuts at: 0 1265 2973
Size: 1265 1708
BmtI G_CTAG'C
Cuts at: 0 956 2973
Size: 956 2017
Bpu10I CC'TnA_GC
Cuts at: 0 2692 2973
Size: 2692 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1558 2171 2348 2534 2973
Size: 905 653 613 177 186 439
Fragments arranged by size:
905 653 613 439 186 177
BsaI GGTCTCn'nnnn_
Cuts at: 0 2366 2973
Size: 2366 607
BsaBI GATnn'nnATC
Cuts at: 0 299 2973
Size: 299 2674
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2056 2086 2973
Size: 2056 30 887
Fragments arranged by size:
2056 887 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1939 2973
Size: 19 1920 1034
Fragments arranged by size:
1920 1034 19
BseYI C'CCAG_C
Cuts at: 0 427 1991 2973
Size: 427 1564 982
Fragments arranged by size:
1564 982 427
BsmI GAATG_Cn'
Cuts at: 0 2798 2871 2973
Size: 2798 73 102
Fragments arranged by size:
2798 102 73
Bsp1286I G_dGCh'C
Cuts at: 0 1265 2973
Size: 1265 1708
BspHI T'CATG_A
Cuts at: 0 2610 2661 2973
Size: 2610 51 312
Fragments arranged by size:
2610 312 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 201 2973
Size: 201 2772
BsrDI GCAATG_nn'
Cuts at: 0 523 1226 2096 2973
Size: 523 703 870 877
Fragments arranged by size:
877 870 703 523
BssSI C'ACGA_G
Cuts at: 0 239 2720 2973
Size: 239 2481 253
Fragments arranged by size:
2481 253 239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 286 1845 2973
Size: 286 1559 1128
Fragments arranged by size:
1559 1128 286
BstBI TT'CG_AA
Cuts at: 0 961 2973
Size: 961 2012
BstYI r'GATC_y
Cuts at: 0 633 663 1870 2973
Size: 633 30 1207 1103
Fragments arranged by size:
1207 1103 633 30
Bsu36I CC'TnA_GG
Cuts at: 0 1865 2973
Size: 1865 1108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1513 2973
Size: 1513 1460
BtsI GCAGTG_nn'
Cuts at: 0 136 2135 2417 2973
Size: 136 1999 282 556
Fragments arranged by size:
1999 556 282 136
DraI TTT'AAA
Cuts at: 0 1023 2973
Size: 1023 1950
EaeI y'GGCC_r
Cuts at: 0 987 2930 2973
Size: 987 1943 43
Fragments arranged by size:
1943 987 43
EarI CTCTTCn'nnn_
Cuts at: 0 576 1963 2051 2337 2973
Size: 576 1387 88 286 636
Fragments arranged by size:
1387 636 576 286 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 667 1361 1376 2479 2548 2830 2973
Size: 557 110 694 15 1103 69 282 143
Fragments arranged by size:
1103 694 557 282 143 110 69 15
EcoRI G'AATT_C
Cuts at: 0 2494 2973
Size: 2494 479
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 610 2443 2973
Size: 610 1833 530
Fragments arranged by size:
1833 610 530
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 2973
Size: 110 32 2831
Fragments arranged by size:
2831 110 32
HincII GTy'rAC
Cuts at: 0 216 839 1451 1884 2973
Size: 216 623 612 433 1089
Fragments arranged by size:
1089 623 612 433 216
HindIII A'AGCT_T
Cuts at: 0 227 412 554 2973
Size: 227 185 142 2419
Fragments arranged by size:
2419 227 185 142
HpaI GTT'AAC
Cuts at: 0 216 1451 1884 2973
Size: 216 1235 433 1089
Fragments arranged by size:
1235 1089 433 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2093 2973
Size: 2093 880
MscI TGG'CCA
Cuts at: 0 989 2973
Size: 989 1984
MslI CAynn'nnrTG
Cuts at: 0 937 1152 1258 1699 2973
Size: 937 215 106 441 1274
Fragments arranged by size:
1274 937 441 215 106
MspA1I CmG'CkG
Cuts at: 0 2098 2638 2737 2973
Size: 2098 540 99 236
Fragments arranged by size:
2098 540 236 99
NdeI CA'TA_TG
Cuts at: 0 1080 1521 2973
Size: 1080 441 1452
Fragments arranged by size:
1452 1080 441
NheI G'CTAG_C
Cuts at: 0 952 2973
Size: 952 2021
PflMI CCAn_nnn'nTGG
Cuts at: 0 1555 2973
Size: 1555 1418
PfoI T'CCnGG_A
Cuts at: 0 245 1138 2973
Size: 245 893 1835
Fragments arranged by size:
1835 893 245
PshAI GACnn'nnGTC
Cuts at: 0 241 2973
Size: 241 2732
PsiI TTA'TAA
Cuts at: 0 1168 2973
Size: 1168 1805
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1 33 2973
Size: 1 32 2940
Fragments arranged by size:
2940 32 1
PstI C_TGCA'G
Cuts at: 0 408 1606 2412 2973
Size: 408 1198 806 561
Fragments arranged by size:
1198 806 561 408
PvuII CAG'CTG
Cuts at: 0 2638 2737 2973
Size: 2638 99 236
Fragments arranged by size:
2638 236 99
SapI GCTCTTCn'nnn_
Cuts at: 0 2051 2337 2973
Size: 2051 286 636
Fragments arranged by size:
2051 636 286
SfcI C'TryA_G
Cuts at: 0 404 1602 2408 2973
Size: 404 1198 806 565
Fragments arranged by size:
1198 806 565 404
SmlI C'TyrA_G
Cuts at: 0 920 1573 2186 2363 2549 2973
Size: 920 653 613 177 186 424
Fragments arranged by size:
920 653 613 424 186 177
SpeI A'CTAG_T
Cuts at: 0 1906 2302 2973
Size: 1906 396 671
Fragments arranged by size:
1906 671 396
SspI AAT'ATT
Cuts at: 0 534 2973
Size: 534 2439
StuI AGG'CCT
Cuts at: 0 2334 2973
Size: 2334 639
StyI C'CwwG_G
Cuts at: 0 906 2856 2973
Size: 906 1950 117
Fragments arranged by size:
1950 906 117
Tth111I GACn'n_nGTC
Cuts at: 0 737 2973
Size: 737 2236
XbaI T'CTAG_A
Cuts at: 0 730 1002 2973
Size: 730 272 1971
Fragments arranged by size:
1971 730 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2285 2973
Size: 2285 688
XmnI GAAnn'nnTTC
Cuts at: 0 557 1134 1908 2973
Size: 557 577 774 1065
Fragments arranged by size:
1065 774 577 557
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApoI BbsI
BclI BlpI Bme1580I BmtI Bpu10I BpuEI BsaI BsaBI
BsaXI BseRI BseYI BsmI Bsp1286I BspHI BspMI BsrDI
BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI
EaeI EarI Eco57MI EcoRI FalI Hin4I HincII HindIII
HpaI MmeI MscI MslI MspA1I NdeI NheI PflMI
PfoI PshAI PsiI PsrI PstI PvuII SapI SfcI
SmlI SpeI SspI StuI StyI Tth111I XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BmgBI BmrI BplI BpmI BsaAI
BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BstEII BstXI BstZ17I BtgI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI
MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PpuMI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI TaqII TatI TstI
XhoI XmaI ZraI