(Circular) (Six-base) MAPSORT of: pFN21AA0391.seq Check: 1192 from: 1 to: 6406 pFN21AA0391 6406 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2325 2601 4469 5853 6121 1632 Size: 693 276 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 693 276 268 AflII C'TTAA_G Cuts at: 820 1017 2384 3059 3128 820 Size: 197 1367 675 69 4098 Fragments arranged by size: 4098 1367 675 197 69 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 3278 5135 3278 Size: 1857 4549 AleI CACnn'nnGTG Cuts at: 1166 3599 1166 Size: 2433 3973 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 4149 5721 4149 Size: 1572 4834 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2293 2362 2797 3715 3941 4093 6202 1493 Size: 800 69 435 918 226 152 2109 1697 Fragments arranged by size: 2109 1697 918 800 435 226 152 69 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 3731 1870 Size: 73 1788 4545 Fragments arranged by size: 4545 1788 73 AvrII C'CTAG_G Cuts at: 3283 3283 Size: 6406 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2320 3727 5182 618 Size: 325 217 627 533 1407 1455 1842 Fragments arranged by size: 1842 1455 1407 627 533 325 217 BanII G_rGCy'C Cuts at: 1391 2057 2767 3725 1391 Size: 666 710 958 4072 Fragments arranged by size: 4072 958 710 666 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2639 3648 928 Size: 971 740 1009 3686 Fragments arranged by size: 3686 1009 971 740 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2765 2955 4298 5508 2765 Size: 190 1343 1210 3663 Fragments arranged by size: 3663 1343 1210 190 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5017 136 Size: 107 122 71 631 605 3345 1525 Fragments arranged by size: 3345 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2082 4467 5623 1790 Size: 292 2385 1156 2573 Fragments arranged by size: 2573 2385 1156 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3310 4137 5095 Size: 416 53 83 399 24 1893 827 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1893 1753 958 827 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 3633 5066 1239 Size: 1047 1347 1433 2579 Fragments arranged by size: 2579 1433 1347 1047 Bpu10I CC'TnA_GC Cuts at: 2274 2274 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4531 5396 5694 4531 Size: 865 298 5243 Fragments arranged by size: 5243 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3191 3444 5069 882 Size: 617 1692 253 1625 2219 Fragments arranged by size: 2219 1692 1625 617 253 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4213 4595 5230 275 Size: 53 83 186 936 2680 382 635 1451 Fragments arranged by size: 2680 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2408 4839 5237 5511 5658 6069 2408 Size: 2431 398 274 147 411 2745 Fragments arranged by size: 2745 2431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2143 2173 1178 Size: 30 165 30 740 30 5411 Fragments arranged by size: 5411 740 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 1186 Size: 1198 5208 BseYI C'CCAG_C Cuts at: 5609 6105 5609 Size: 496 5910 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3401 1163 Size: 573 1665 4168 Fragments arranged by size: 4168 1665 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2767 4467 4552 5623 2767 Size: 1700 85 1071 3550 Fragments arranged by size: 3550 1700 1071 85 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 BsmBI CGTCTCn'nnnn_ Cuts at: 3250 3250 Size: 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2082 2767 3725 4467 4552 5623 Size: 399 267 25 685 958 742 85 1071 Cuts at: 5623 1391 Size: 2174 Fragments arranged by size: 2174 1071 958 742 685 399 267 85 25 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 2821 3072 4295 6025 2821 Size: 251 1223 1730 3202 Fragments arranged by size: 3202 1730 1223 251 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3426 3761 844 Size: 2582 335 3489 Fragments arranged by size: 3489 2582 335 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4901 5075 65 Size: 1183 3653 174 1396 Fragments arranged by size: 3653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5050 5237 1700 Size: 110 3240 187 2869 Fragments arranged by size: 3240 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 3619 4466 5478 3619 Size: 847 1012 4547 Fragments arranged by size: 4547 1012 847 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6406 BstBI TT'CG_AA Cuts at: 2029 2424 5210 2029 Size: 395 2786 3225 Fragments arranged by size: 3225 2786 395 BstEII G'GTnAC_C Cuts at: 1184 3095 1184 Size: 1911 4495 BstXI CCAn_nnnn'nTGG Cuts at: 3681 3681 Size: 6406 BstYI r'GATC_y Cuts at: 1972 3736 4103 4488 4505 5957 6043 6055 6401 Size: 1764 367 385 17 1452 86 12 346 Cuts at: 6401 1972 Size: 1977 Fragments arranged by size: 1977 1764 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3120 3120 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5854 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 3413 3430 3922 4716 4736 3413 Size: 17 492 794 20 5083 Fragments arranged by size: 5083 794 492 20 17 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 2397 2850 3710 2397 Size: 453 860 5093 Fragments arranged by size: 5093 860 453 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2992 3853 4742 Size: 54 1425 50 275 18 1162 861 889 Cuts at: 4742 6371 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1162 889 861 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3333 4336 1353 Size: 692 1288 1003 3423 Fragments arranged by size: 3423 1288 1003 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2765 2765 Size: 6406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2325 2601 3633 4469 5066 5853 Size: 393 654 39 276 1032 836 597 787 Cuts at: 5853 6121 1239 Size: 268 1524 Fragments arranged by size: 1524 1032 836 787 654 597 393 276 268 39 EcoRI G'AATT_C Cuts at: 2293 2362 3715 4093 2293 Size: 69 1353 378 4606 Fragments arranged by size: 4606 1353 378 69 EcoRV GAT'ATC Cuts at: 2204 2204 Size: 6406 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 5553 1678 Size: 3875 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2281 2313 3103 3135 3264 Size: 32 135 32 709 32 790 32 129 Cuts at: 3264 3296 3365 3397 5047 5079 5121 5153 1373 Size: 32 69 32 1650 32 42 32 2626 Fragments arranged by size: 2626 1650 790 709 135 129 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 2032 2042 2853 748 Size: 1284 10 811 4301 Fragments arranged by size: 4301 1284 811 10 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 3537 4005 3537 Size: 468 5938 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2411 2820 5520 5704 1305 Size: 1106 409 2700 184 2007 Fragments arranged by size: 2700 2007 1106 409 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2994 10 Size: 54 1425 343 1162 3422 Fragments arranged by size: 3422 1425 1162 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 2778 3599 4364 4723 4882 518 Size: 648 919 693 821 765 359 159 2042 Fragments arranged by size: 2042 919 821 765 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2112 2941 3034 3360 3532 3822 4499 5647 Size: 506 829 93 326 172 290 677 1148 Cuts at: 5647 5892 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 829 677 506 326 290 245 172 93 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5854 NdeI CA'TA_TG Cuts at: 387 2568 3634 387 Size: 2181 1066 3159 Fragments arranged by size: 3159 2181 1066 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3301 3764 5309 1132 Size: 97 2072 463 1545 2229 Fragments arranged by size: 2229 2072 1545 463 97 PasI CC'CwG_GG Cuts at: 1348 2075 1348 Size: 727 5679 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2063 1108 Size: 955 5451 PfoI T'CCnGG_A Cuts at: 3422 3553 3422 Size: 131 6275 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 2723 3976 2723 Size: 1253 5153 PstI C_TGCA'G Cuts at: 830 3437 3758 830 Size: 2607 321 3478 Fragments arranged by size: 3478 2607 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2941 3034 3360 3532 2941 Size: 93 326 172 5815 Fragments arranged by size: 5815 326 172 93 SacI G_AGCT'C Cuts at: 2767 2767 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 2045 2045 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 2466 3433 3754 4889 5570 5761 826 Size: 219 1421 967 321 1135 681 191 1471 Fragments arranged by size: 1471 1421 1135 967 681 321 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3059 3128 4510 5411 5673 Size: 197 926 441 675 69 1382 901 262 Cuts at: 5673 820 Size: 1553 Fragments arranged by size: 1553 1382 926 901 675 441 262 197 69 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 3473 1517 Size: 1956 4450 StyI C'CwwG_G Cuts at: 513 1065 2062 2193 2316 3283 3845 513 Size: 552 997 131 123 967 562 3074 Fragments arranged by size: 3074 997 967 562 552 131 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4433 4450 4603 4788 5233 325 Size: 1255 2853 17 153 185 445 1498 Fragments arranged by size: 2853 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3102 4652 6364 Size: 274 80 33 51 1443 1125 1550 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 1712 1550 1443 1125 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6366 6398 6366 Size: 32 6374 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI