Restriction map for pFN21AA0391
 (Circular) (Six-base) MAPSORT of: pFN21AA0391.seq  Check: 1192  from: 1  to: 6406



pFN21AA0391  6406 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:22  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4216     278 
   Size:         53      83     186     936    2680    2468

  Fragments arranged by size: 

                2680    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3749    1937 
   Size:       1812    4594
 
Acc65I G'GTAC_C

Cuts at:   3727    3727 
   Size:       6406
 
AclI AA'CG_TT

Cuts at:   1563    4533    4906    1563 
   Size:       2970     373    3063

  Fragments arranged by size: 

                3063    2970     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2325    2601    4469    5853    6121    1632 
   Size:        693     276    1868    1384     268    1917

  Fragments arranged by size: 

                1917    1868    1384     693     276     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2384    3059    3128     820 
   Size:        197    1367     675      69    4098

  Fragments arranged by size: 

                4098    1367     675     197      69
 
AflIII A'CryG_T

Cuts at:   1203    5305    6282    1203 
   Size:       4102     977    1327

  Fragments arranged by size: 

                4102    1327     977
 
AgeI A'CCGG_T

Cuts at:   5237    5237 
   Size:       6406
 
AhdI GACnn_n'nnGTC

Cuts at:   3278    5135    3278 
   Size:       1857    4549
 
AleI CACnn'nnGTG

Cuts at:   1166    3599    1166 
   Size:       2433    3973
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6406
 
AlwNI CAG_nnn'CTG

Cuts at:   4149    5721    4149 
   Size:       1572    4834
 
ApaLI G'TGCA_C

Cuts at:   4463    5619    4463 
   Size:       1156    5250
 
ApoI r'AATT_y

Cuts at:   1493    2293    2362    2797    3715    3941    4093    6202    1493 
   Size:        800      69     435     918     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     918     800     435     226     152      69
            
 
AseI AT'TA_AT

Cuts at:    160    1032    4960     160 
   Size:        872    3928    1606

  Fragments arranged by size: 

                3928    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6406
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3731    1870 
   Size:         73    1788    4545

  Fragments arranged by size: 

                4545    1788      73
 
AvrII C'CTAG_G

Cuts at:   3283    3283 
   Size:       6406
 
BamHI G'GATC_C

Cuts at:   3736    4103    3736 
   Size:        367    6039
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2320    3727    5182     618 
   Size:        325     217     627     533    1407    1455    1842

  Fragments arranged by size: 

                1842    1455    1407     627     533     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2057    2767    3725    1391 
   Size:        666     710     958    4072

  Fragments arranged by size: 

                4072     958     710     666
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2639    3648     928 
   Size:        971     740    1009    3686

  Fragments arranged by size: 

                3686    1009     971     740
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4597    4631    1903 
   Size:         34    2660      34    3678

  Fragments arranged by size: 

                3678    2660      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2765    2955    4298    5508    2765 
   Size:        190    1343    1210    3663

  Fragments arranged by size: 

                3663    1343    1210     190
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6406
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    5017     136 
   Size:        107     122      71     631     605    3345    1525

  Fragments arranged by size: 

                3345    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   6401    6401 
   Size:       6406
 
BlpI GC'TnA_GC

Cuts at:   3823    3823 
   Size:       6406
 
Bme1580I G_kGCm'C

Cuts at:   1790    2082    4467    5623    1790 
   Size:        292    2385    1156    2573

  Fragments arranged by size: 

                2573    2385    1156     292
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6406
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3310    4137    5095 
   Size:        416      53      83     399      24    1893     827     958

Cuts at:   5095     442 
   Size:       1753

  Fragments arranged by size: 

                1893    1753     958     827     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6406
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2286    3633    5066    1239 
   Size:       1047    1347    1433    2579

  Fragments arranged by size: 

                2579    1433    1347    1047
 
Bpu10I CC'TnA_GC

Cuts at:   2274    2274 
   Size:       6406
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4531    5396    5694    4531 
   Size:        865     298    5243

  Fragments arranged by size: 

                5243     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3191    3444    5069     882 
   Size:        617    1692     253    1625    2219

  Fragments arranged by size: 

                2219    1692    1625     617     253
 
BsaAI yAC'GTr

Cuts at:    493    1204    4197     493 
   Size:        711    2993    2702

  Fragments arranged by size: 

                2993    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6400    6400 
   Size:       6406
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4213    4595    5230     275 
   Size:         53      83     186     936    2680     382     635    1451

  Fragments arranged by size: 

                2680    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2408    4839    5237    5511    5658    6069    2408 
   Size:       2431     398     274     147     411    2745

  Fragments arranged by size: 

                2745    2431     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2143    2173    1178 
   Size:         30     165      30     740      30    5411

  Fragments arranged by size: 

                5411     740     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2384    1186 
   Size:       1198    5208
 
BseYI C'CCAG_C

Cuts at:   5609    6105    5609 
   Size:        496    5910
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3401    1163 
   Size:        573    1665    4168

  Fragments arranged by size: 

                4168    1665     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3856    4617    5645    1540 
   Size:        275     186    1855     761    1028    2301

  Fragments arranged by size: 

                2301    1855    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2767    4467    4552    5623    2767 
   Size:       1700      85    1071    3550

  Fragments arranged by size: 

                3550    1700    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3917    4010    3917 
   Size:         93    6313
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3250    3250 
   Size:       6406
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2057    2082    2767    3725    4467    4552    5623 
   Size:        399     267      25     685     958     742      85    1071

Cuts at:   5623    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     958     742     685     399     267      85
                  25
 
BspEI T'CCGG_A

Cuts at:   6069    6069 
   Size:       6406
 
BspHI T'CATG_A

Cuts at:   2821    3072    4295    6025    2821 
   Size:        251    1223    1730    3202

  Fragments arranged by size: 

                3202    1730    1223     251
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3426    3761     844 
   Size:       2582     335    3489

  Fragments arranged by size: 

                3489    2582     335
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4293    1394 
   Size:        429    2470    3507

  Fragments arranged by size: 

                3507    2470     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4901    5075      65 
   Size:       1183    3653     174    1396

  Fragments arranged by size: 

                3653    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5050    5237    1700 
   Size:        110    3240     187    2869

  Fragments arranged by size: 

                3240    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6406
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6406
 
BssSI C'ACGA_G

Cuts at:   3619    4466    5478    3619 
   Size:        847    1012    4547

  Fragments arranged by size: 

                4547    1012     847
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6406
 
BstBI TT'CG_AA

Cuts at:   2029    2424    5210    2029 
   Size:        395    2786    3225

  Fragments arranged by size: 

                3225    2786     395
 
BstEII G'GTnAC_C

Cuts at:   1184    3095    1184 
   Size:       1911    4495
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3681    3681 
   Size:       6406
 
BstYI r'GATC_y

Cuts at:   1972    3736    4103    4488    4505    5957    6043    6055    6401 
   Size:       1764     367     385      17    1452      86      12     346

Cuts at:   6401    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1764    1452     385     367     346      86      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   3120    3120 
   Size:       6406
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    5854
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5667

  Fragments arranged by size: 

                5667     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3413    3430    3922    4716    4736    3413 
   Size:         17     492     794      20    5083

  Fragments arranged by size: 

                5083     794     492      20      17
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6406
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6371
 
DraI TTT'AAA

Cuts at:   2397    2850    3710    2397 
   Size:        453     860    5093

  Fragments arranged by size: 

                5093     860     453
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5413     809 
   Size:        507    4097    1802

  Fragments arranged by size: 

                4097    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2992    3853    4742 
   Size:         54    1425      50     275      18    1162     861     889

Cuts at:   4742    6371       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1162     889     861     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3853    1537 
   Size:        275    2041    4090

  Fragments arranged by size: 

                4090    2041     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2045    3333    4336    1353 
   Size:        692    1288    1003    3423

  Fragments arranged by size: 

                3423    1288    1003     692
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4993    5367    5513     387 
   Size:       1064     138    3404     374     146    1280

  Fragments arranged by size: 

                3404    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2765    2765 
   Size:       6406
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2286    2325    2601    3633    4469    5066    5853 
   Size:        393     654      39     276    1032     836     597     787

Cuts at:   5853    6121    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1032     836     787     654     597     393     276
                 268      39
 
EcoRI G'AATT_C

Cuts at:   2293    2362    3715    4093    2293 
   Size:         69    1353     378    4606

  Fragments arranged by size: 

                4606    1353     378      69
 
EcoRV GAT'ATC

Cuts at:   2204    2204 
   Size:       6406
 
FspI TGC'GCA

Cuts at:   4912    4912 
   Size:       6406
 
HaeII r_GCGC'y

Cuts at:   1678    5553    1678 
   Size:       3875    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2281    2313    3103    3135    3264 
   Size:         32     135      32     709      32     790      32     129

Cuts at:   3264    3296    3365    3397    5047    5079    5121    5153    1373 
   Size:         32      69      32    1650      32      42      32    2626

  Fragments arranged by size: 

                2626    1650     790     709     135     129      69      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3750    3996     669 
   Size:        988     281    1812     246    3079

  Fragments arranged by size: 

                3079    1812     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2032    2042    2853     748 
   Size:       1284      10     811    4301

  Fragments arranged by size: 

                4301    1284     811      10
 
HpaI GTT'AAC

Cuts at:   3996    3996 
   Size:       6406
 
KpnI G_GTAC'C

Cuts at:   3731    3731 
   Size:       6406
 
MfeI C'AATT_G

Cuts at:   3537    4005    3537 
   Size:        468    5938
 
MluI A'CGCG_T

Cuts at:   6282    6282 
   Size:       6406
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2411    2820    5520    5704    1305 
   Size:       1106     409    2700     184    2007

  Fragments arranged by size: 

                2700    2007    1106     409     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2994      10 
   Size:         54    1425     343    1162    3422

  Fragments arranged by size: 

                3422    1425    1162     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2085    2778    3599    4364    4723    4882     518 
   Size:        648     919     693     821     765     359     159    2042

  Fragments arranged by size: 

                2042     919     821     765     693     648     359     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2112    2941    3034    3360    3532    3822    4499    5647 
   Size:        506     829      93     326     172     290     677    1148

Cuts at:   5647    5892    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1148     829     677     506     326     290     245
                 172      93
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5854
 
NdeI CA'TA_TG

Cuts at:    387    2568    3634     387 
   Size:       2181    1066    3159

  Fragments arranged by size: 

                3159    2181    1066
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6406
 
NotI GC'GGCC_GC

Cuts at:   3853    3853 
   Size:       6406
 
NspI r_CATG'y

Cuts at:   1132    1229    3301    3764    5309    1132 
   Size:         97    2072     463    1545    2229

  Fragments arranged by size: 

                2229    2072    1545     463      97
 
PasI CC'CwG_GG

Cuts at:   1348    2075    1348 
   Size:        727    5679
 
PciI A'CATG_T

Cuts at:   5305    5305 
   Size:       6406
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2063    1108 
   Size:        955    5451
 
PfoI T'CCnGG_A

Cuts at:   3422    3553    3422 
   Size:        131    6275
 
PmeI GTTT'AAAC

Cuts at:   3710    3710 
   Size:       6406
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4475    4507    5988    6020    4475 
   Size:         32    1481      32    4861

  Fragments arranged by size: 

                4861    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6406
 
PsiI TTA'TAA

Cuts at:   2723    3976    2723 
   Size:       1253    5153
 
PstI C_TGCA'G

Cuts at:    830    3437    3758     830 
   Size:       2607     321    3478

  Fragments arranged by size: 

                3478    2607     321
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6406
 
PvuII CAG'CTG

Cuts at:   2941    3034    3360    3532    2941 
   Size:         93     326     172    5815

  Fragments arranged by size: 

                5815     326     172      93
 
SacI G_AGCT'C

Cuts at:   2767    2767 
   Size:       6406
 
SalI G'TCGA_C

Cuts at:   1936    3748    1936 
   Size:       1812    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2045    2045 
   Size:       6406
 
SbfI CC_TGCA'GG

Cuts at:   3758    3758 
   Size:       6406
 
ScaI AGT'ACT

Cuts at:   4654    6366    4654 
   Size:       1712    4694
 
SfcI C'TryA_G

Cuts at:    826    1045    2466    3433    3754    4889    5570    5761     826 
   Size:        219    1421     967     321    1135     681     191    1471

  Fragments arranged by size: 

                1471    1421    1135     967     681     321     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6406
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6406
 
SmaI CCC'GGG

Cuts at:   1872    3733    1872 
   Size:       1861    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2384    3059    3128    4510    5411    5673 
   Size:        197     926     441     675      69    1382     901     262

Cuts at:   5673     820 
   Size:       1553

  Fragments arranged by size: 

                1553    1382     926     901     675     441     262     197
                  69
 
SnaBI TAC'GTA

Cuts at:    493    4197     493 
   Size:       3704    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6406
 
SphI G_CATG'C

Cuts at:   1132    3764    1132 
   Size:       2632    3774
 
SspI AAT'ATT

Cuts at:      5      52    4330       5 
   Size:         47    4278    2081

  Fragments arranged by size: 

                4278    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3473    1517 
   Size:       1956    4450
 
StyI C'CwwG_G

Cuts at:    513    1065    2062    2193    2316    3283    3845     513 
   Size:        552     997     131     123     967     562    3074

  Fragments arranged by size: 

                3074     997     967     562     552     131     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4433    4450    4603    4788    5233     325 
   Size:       1255    2853      17     153     185     445    1498

  Fragments arranged by size: 

                2853    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3102    4652    6364 
   Size:        274      80      33      51    1443    1125    1550    1712

Cuts at:   6364      96 
   Size:        138

  Fragments arranged by size: 

                1712    1550    1443    1125     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6366    6398    6366 
   Size:         32    6374
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6406
 
XbaI T'CTAG_A

Cuts at:   3742    3742 
   Size:       6406
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6406
 
XmaI C'CCGG_G

Cuts at:   1870    3731    1870 
   Size:       1861    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4535    4535 
   Size:       6406
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4214     276 
   Size:         53      83     186     936    2680    2468

  Fragments arranged by size: 

                2680    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
     PsiI     PstI     PvuI    PvuII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI  BstZ17I   DraIII    EcoNI EcoO109I     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII    SacII
    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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