(Circular) (Six-base) MAPSORT of: pFN21AA0197.seq Check: 4381 from: 1 to: 8650 pFN21AA0197 8650 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6460 278 Size: 53 83 186 936 4924 2468 Fragments arranged by size: 4924 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4766 5993 1937 Size: 2829 1227 4594 Fragments arranged by size: 4594 2829 1227 Acc65I G'GTAC_C Cuts at: 3588 5971 3588 Size: 2383 6267 AclI AA'CG_TT Cuts at: 1563 6777 7150 1563 Size: 5214 373 3063 Fragments arranged by size: 5214 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2727 3401 3491 4725 4884 6713 8097 8365 Size: 1095 674 90 1234 159 1829 1384 268 Cuts at: 8365 1632 Size: 1917 Fragments arranged by size: 1917 1829 1384 1234 1095 674 268 159 90 AflII C'TTAA_G Cuts at: 820 1017 2621 820 Size: 197 1604 6849 Fragments arranged by size: 6849 1604 197 AflIII A'CryG_T Cuts at: 1203 4904 7549 8526 1203 Size: 3701 2645 977 1327 Fragments arranged by size: 3701 2645 1327 977 AgeI A'CCGG_T Cuts at: 7481 7481 Size: 8650 AhdI GACnn_n'nnGTC Cuts at: 7379 7379 Size: 8650 AleI CACnn'nnGTG Cuts at: 1166 5823 1166 Size: 4657 3993 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4073 5672 1184 Size: 2889 1599 4162 Fragments arranged by size: 4162 2889 1599 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 2601 Size: 32 8618 AlwNI CAG_nnn'CTG Cuts at: 2522 3217 3752 3826 4117 4319 4703 5339 5519 Size: 695 535 74 291 202 384 636 180 Cuts at: 5519 6393 7965 2522 Size: 874 1572 3207 Fragments arranged by size: 3207 1572 874 695 636 535 384 291 202 180 74 ApaI G_GGCC'C Cuts at: 3124 3124 Size: 8650 ApaLI G'TGCA_C Cuts at: 2381 6707 7863 2381 Size: 4326 1156 3168 Fragments arranged by size: 4326 3168 1156 ApoI r'AATT_y Cuts at: 1493 2081 2291 3355 3440 3463 3476 4721 4968 Size: 588 210 1064 85 23 13 1245 247 Cuts at: 4968 5959 6185 6337 8446 1493 Size: 991 226 152 2109 1697 Fragments arranged by size: 2109 1697 1245 1064 991 588 247 226 210 152 85 23 13 AseI AT'TA_AT Cuts at: 160 1032 7204 160 Size: 872 6172 1606 Fragments arranged by size: 6172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8650 AvaI C'yCGr_G Cuts at: 1870 1943 3699 5975 1870 Size: 73 1756 2276 4545 Fragments arranged by size: 4545 2276 1756 73 BamHI G'GATC_C Cuts at: 5980 6347 5980 Size: 367 8283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 3588 4432 5971 7426 Size: 325 217 627 336 1465 844 1539 1455 Cuts at: 7426 618 Size: 1842 Fragments arranged by size: 1842 1539 1465 1455 844 627 336 325 217 BanII G_rGCy'C Cuts at: 1391 2020 3124 5969 1391 Size: 629 1104 2845 4072 Fragments arranged by size: 4072 2845 1104 629 BbeI G_GCGC'C Cuts at: 2127 2127 Size: 8650 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2222 3497 928 Size: 971 323 1275 6081 Fragments arranged by size: 6081 1275 971 323 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6841 6875 1903 Size: 34 4904 34 3678 Fragments arranged by size: 4904 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6542 7752 6542 Size: 1210 7440 BclI T'GATC_A Cuts at: 1548 3247 3837 1548 Size: 1699 590 6361 Fragments arranged by size: 6361 1699 590 BfrBI ATG'CAT Cuts at: 2980 5649 2980 Size: 2669 5981 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5869 7261 Size: 107 122 71 631 605 337 3860 1392 Cuts at: 7261 136 Size: 1525 Fragments arranged by size: 3860 1525 1392 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4096 5443 8645 4096 Size: 1347 3202 4101 Fragments arranged by size: 4101 3202 1347 BlpI GC'TnA_GC Cuts at: 2042 2052 2561 3981 4173 5490 6067 2042 Size: 10 509 1420 192 1317 577 4625 Fragments arranged by size: 4625 1420 1317 577 509 192 10 Bme1580I G_kGCm'C Cuts at: 1790 2385 3124 6711 7867 1790 Size: 595 739 3587 1156 2573 Fragments arranged by size: 3587 2573 1156 739 595 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6381 7339 442 Size: 416 53 83 399 24 4964 958 1753 Fragments arranged by size: 4964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5705 1056 Size: 4649 4001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2244 2337 2894 3391 4295 5382 5468 5807 Size: 1005 93 557 497 904 1087 86 339 Cuts at: 5807 7310 1239 Size: 1503 2579 Fragments arranged by size: 2579 1503 1087 1005 904 557 497 339 93 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5273 6775 7640 7938 5273 Size: 1502 865 298 5985 Fragments arranged by size: 5985 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7313 882 Size: 617 5814 2219 Fragments arranged by size: 5814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2164 5105 6441 493 Size: 711 960 2941 1336 2702 Fragments arranged by size: 2941 2702 1336 960 711 BsaBI GATnn'nnATC Cuts at: 3236 8644 3236 Size: 5408 3242 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 6457 6839 7474 Size: 53 83 186 936 591 4333 382 635 Cuts at: 7474 275 Size: 1451 Fragments arranged by size: 4333 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3802 7083 7481 7755 7902 8313 3802 Size: 3281 398 274 147 411 4139 Fragments arranged by size: 4139 3281 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 2834 2864 4125 Size: 30 165 30 1164 30 237 30 1261 Cuts at: 4125 4155 4748 4778 5441 5471 1178 Size: 30 593 30 663 30 4357 Fragments arranged by size: 4357 1261 1164 663 593 237 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 3467 5682 1186 Size: 1075 1206 2215 4154 Fragments arranged by size: 4154 2215 1206 1075 BseYI C'CCAG_C Cuts at: 4011 4636 7853 8349 4011 Size: 625 3217 496 4312 Fragments arranged by size: 4312 3217 625 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4069 5348 5364 1163 Size: 573 2333 1279 16 4449 Fragments arranged by size: 4449 2333 1279 573 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6100 6861 7889 1540 Size: 275 186 4099 761 1028 2301 Fragments arranged by size: 4099 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 3026 6711 6796 7867 2385 Size: 641 3685 85 1071 3168 Fragments arranged by size: 3685 3168 1071 641 85 BsmI GAATG_Cn' Cuts at: 5220 5399 6161 6254 5220 Size: 179 762 93 7616 Fragments arranged by size: 7616 762 179 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2385 3026 3124 5969 6711 6796 Size: 399 230 365 641 98 2845 742 85 Cuts at: 6796 7867 1391 Size: 1071 2174 Fragments arranged by size: 2845 2174 1071 742 641 399 365 230 98 85 BspEI T'CCGG_A Cuts at: 3802 8313 3802 Size: 4511 4139 BspHI T'CATG_A Cuts at: 5383 6539 8269 5383 Size: 1156 1730 5764 Fragments arranged by size: 5764 1730 1156 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3176 6005 844 Size: 2332 2829 3489 Fragments arranged by size: 3489 2829 2332 BsrBI CCG'CTC Cuts at: 1394 1823 6537 1394 Size: 429 4714 3507 Fragments arranged by size: 4714 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7145 7319 65 Size: 1183 5897 174 1396 Fragments arranged by size: 5897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7294 7481 1700 Size: 110 5484 187 2869 Fragments arranged by size: 5484 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4564 96 Size: 4468 4182 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8650 BssSI C'ACGA_G Cuts at: 6710 7722 6710 Size: 1012 7638 BstAPI GCAn_nnn'nTGC Cuts at: 794 2695 3217 794 Size: 1901 522 6227 Fragments arranged by size: 6227 1901 522 BstBI TT'CG_AA Cuts at: 3460 3997 5236 7454 3460 Size: 537 1239 2218 4656 Fragments arranged by size: 4656 2218 1239 537 BstEII G'GTnAC_C Cuts at: 1184 2729 1184 Size: 1545 7105 BstXI CCAn_nnnn'nTGG Cuts at: 3914 4019 5785 3914 Size: 105 1766 6779 Fragments arranged by size: 6779 1766 105 BstYI r'GATC_y Cuts at: 1972 2488 3361 4096 5044 5443 5980 6347 6732 Size: 516 873 735 948 399 537 367 385 Cuts at: 6732 6749 8201 8287 8299 8645 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 948 873 735 537 516 399 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2115 2952 3108 513 Size: 552 939 111 837 156 6055 Fragments arranged by size: 6055 939 837 552 156 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5875 487 Size: 683 56 4649 3262 Fragments arranged by size: 4649 3262 683 56 BtsI GCAGTG_nn' Cuts at: 2311 2572 5352 5577 6166 6960 6980 2311 Size: 261 2780 225 589 794 20 3981 Fragments arranged by size: 3981 2780 794 589 261 225 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2097 2132 529 Size: 35 1533 35 7047 Fragments arranged by size: 7047 1533 35 35 DraI TTT'AAA Cuts at: 5954 5954 Size: 8650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5304 7657 809 Size: 507 3988 2353 1802 Fragments arranged by size: 3988 2353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6097 6986 8615 Size: 54 1425 50 275 18 4267 889 1629 Cuts at: 8615 8 Size: 43 Fragments arranged by size: 4267 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6097 1537 Size: 275 4285 4090 Fragments arranged by size: 4285 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3222 3735 4421 6580 1353 Size: 1869 513 686 2159 3423 Fragments arranged by size: 3423 2159 1869 686 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5465 7237 7611 7757 387 Size: 1064 138 3876 1772 374 146 1280 Fragments arranged by size: 3876 1772 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2337 2727 2894 3391 3401 3491 Size: 393 612 93 390 167 497 10 90 Cuts at: 3491 4295 4725 4884 5382 5468 5807 6713 7310 Size: 804 430 159 498 86 339 906 597 Cuts at: 7310 8097 8365 1239 Size: 787 268 1524 Fragments arranged by size: 1524 906 804 787 612 597 498 497 430 393 390 339 268 167 159 93 90 86 10 EcoO109I rG'GnC_Cy Cuts at: 2578 3121 2578 Size: 543 8107 EcoRI G'AATT_C Cuts at: 2081 3440 3476 4721 5959 6337 2081 Size: 1359 36 1245 1238 378 4394 Fragments arranged by size: 4394 1359 1245 1238 378 36 EcoRV GAT'ATC Cuts at: 3115 3694 3115 Size: 579 8071 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5629 5629 Size: 8650 FspI TGC'GCA Cuts at: 7156 7156 Size: 8650 HaeII r_GCGC'y Cuts at: 1678 2127 7797 1678 Size: 449 5670 2531 Fragments arranged by size: 5670 2531 449 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2329 2361 4748 4780 7291 Size: 32 135 32 757 32 2387 32 2511 Cuts at: 7291 7323 7365 7397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2511 2387 757 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2367 2437 5994 6240 669 Size: 988 281 429 70 3557 246 3079 Fragments arranged by size: 3557 3079 988 429 281 246 70 HindIII A'AGCT_T Cuts at: 748 3309 5606 748 Size: 2561 2297 3792 Fragments arranged by size: 3792 2561 2297 HpaI GTT'AAC Cuts at: 2367 6240 2367 Size: 3873 4777 KasI G'GCGC_C Cuts at: 2123 2123 Size: 8650 KpnI G_GTAC'C Cuts at: 3592 5975 3592 Size: 2383 6267 MfeI C'AATT_G Cuts at: 3912 4568 5287 6249 3912 Size: 656 719 962 6313 Fragments arranged by size: 6313 962 719 656 MluI A'CGCG_T Cuts at: 8526 8526 Size: 8650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4312 5921 7764 7948 1305 Size: 3007 1609 1843 184 2007 Fragments arranged by size: 3007 2007 1843 1609 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6828 Fragments arranged by size: 6828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 5198 5823 6608 6967 7126 518 Size: 648 2397 1635 625 785 359 159 2042 Fragments arranged by size: 2397 2042 1635 785 648 625 359 159 MspA1I CmG'CkG Cuts at: 1606 4117 5402 5507 6066 6743 7891 8136 1606 Size: 2511 1285 105 559 677 1148 245 2120 Fragments arranged by size: 2511 2120 1285 1148 677 559 245 105 NarI GG'CG_CC Cuts at: 2124 2124 Size: 8650 NcoI C'CATG_G Cuts at: 513 1065 2004 2952 513 Size: 552 939 948 6211 Fragments arranged by size: 6211 948 939 552 NdeI CA'TA_TG Cuts at: 387 3710 4951 387 Size: 3323 1241 4086 Fragments arranged by size: 4086 3323 1241 NheI G'CTAG_C Cuts at: 1052 5701 1052 Size: 4649 4001 NotI GC'GGCC_GC Cuts at: 6097 6097 Size: 8650 NsiI A_TGCA'T Cuts at: 2982 5651 2982 Size: 2669 5981 NspI r_CATG'y Cuts at: 1132 1229 2872 2980 2984 4908 6008 7553 1132 Size: 97 1643 108 4 1924 1100 1545 2229 Fragments arranged by size: 2229 1924 1643 1545 1100 108 97 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8650 PciI A'CATG_T Cuts at: 4904 7549 4904 Size: 2645 6005 PflMI CCAn_nnn'nTGG Cuts at: 1108 5339 1108 Size: 4231 4419 PfoI T'CCnGG_A Cuts at: 2408 3368 5430 2408 Size: 960 2062 5628 Fragments arranged by size: 5628 2062 960 PmeI GTTT'AAAC Cuts at: 5954 5954 Size: 8650 PmlI CAC'GTG Cuts at: 5105 5105 Size: 8650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6719 6751 8232 8264 6719 Size: 32 1481 32 7105 Fragments arranged by size: 7105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8650 PsiI TTA'TAA Cuts at: 3719 3770 3789 5876 6220 3719 Size: 51 19 2087 344 6149 Fragments arranged by size: 6149 2087 344 51 19 PspOMI G'GGCC_C Cuts at: 3120 3120 Size: 8650 PstI C_TGCA'G Cuts at: 830 2096 3817 4094 4782 6002 830 Size: 1266 1721 277 688 1220 3478 Fragments arranged by size: 3478 1721 1266 1220 688 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8650 PvuII CAG'CTG Cuts at: 4117 5402 4117 Size: 1285 7365 SalI G'TCGA_C Cuts at: 1936 5992 1936 Size: 4056 4594 SbfI CC_TGCA'GG Cuts at: 6002 6002 Size: 8650 ScaI AGT'ACT Cuts at: 4075 4302 6898 8610 4075 Size: 227 2596 1712 4115 Fragments arranged by size: 4115 2596 1712 227 SexAI A'CCwGG_T Cuts at: 2644 2644 Size: 8650 SfcI C'TryA_G Cuts at: 826 1045 2092 2550 3040 3794 3813 4090 4778 Size: 219 1047 458 490 754 19 277 688 Cuts at: 4778 5039 5751 5998 7133 7814 8005 826 Size: 261 712 247 1135 681 191 1471 Fragments arranged by size: 1471 1135 1047 754 712 688 681 490 458 277 261 247 219 191 19 SfoI GGC'GCC Cuts at: 2125 2125 Size: 8650 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8650 SmaI CCC'GGG Cuts at: 1872 5977 1872 Size: 4105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 5252 6754 7655 7917 820 Size: 197 926 678 2631 1502 901 262 1553 Fragments arranged by size: 2631 1553 1502 926 901 678 262 197 SnaBI TAC'GTA Cuts at: 493 6441 493 Size: 5948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8650 SphI G_CATG'C Cuts at: 1132 2984 6008 1132 Size: 1852 3024 3774 Fragments arranged by size: 3774 3024 1852 SspI AAT'ATT Cuts at: 5 52 2457 3994 6574 5 Size: 47 2405 1537 2580 2081 Fragments arranged by size: 2580 2405 2081 1537 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8650 StyI C'CwwG_G Cuts at: 513 1065 2004 2952 5463 5946 6089 513 Size: 552 939 948 2511 483 143 3074 Fragments arranged by size: 3074 2511 948 939 552 483 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6677 6694 6847 7032 7477 325 Size: 1255 5097 17 153 185 445 1498 Fragments arranged by size: 5097 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4073 4300 4564 Size: 274 80 33 51 1443 2096 227 264 Cuts at: 4564 6896 8608 96 Size: 2332 1712 138 Fragments arranged by size: 2332 2096 1712 1443 274 264 227 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2834 2866 4125 4157 5441 5473 8610 8642 2834 Size: 32 1259 32 1284 32 3137 32 2842 Fragments arranged by size: 3137 2842 1284 1259 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8650 XbaI T'CTAG_A Cuts at: 4534 5986 4534 Size: 1452 7198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5316 5316 Size: 8650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8650 XmaI C'CCGG_G Cuts at: 1870 5975 1870 Size: 4105 4545 XmnI GAAnn'nnTTC Cuts at: 6779 6779 Size: 8650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6458 276 Size: 53 83 186 936 4924 2468 Fragments arranged by size: 4924 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI