(Circular) (Six-base) MAPSORT of: pFN21AA0068.seq Check: 4949 from: 1 to: 8464 pFN21AA0068 8464 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3379 3379 Size: 8464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6274 278 Size: 53 83 186 936 4738 2468 Fragments arranged by size: 4738 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5415 5807 1937 Size: 3478 392 4594 Fragments arranged by size: 4594 3478 392 Acc65I G'GTAC_C Cuts at: 4446 5785 4446 Size: 1339 7125 AclI AA'CG_TT Cuts at: 1563 6591 6964 1563 Size: 5028 373 3063 Fragments arranged by size: 5028 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 3321 4274 4826 4964 4991 5702 6527 Size: 914 775 953 552 138 27 711 825 Cuts at: 6527 7911 8179 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 953 914 825 775 711 552 268 138 27 AfeI AGC'GCT Cuts at: 3298 3298 Size: 8464 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8267 AflIII A'CryG_T Cuts at: 1203 2502 4497 7363 8340 1203 Size: 1299 1995 2866 977 1327 Fragments arranged by size: 2866 1995 1327 1299 977 AgeI A'CCGG_T Cuts at: 4590 7295 4590 Size: 2705 5759 AhdI GACnn_n'nnGTC Cuts at: 2035 2461 7193 2035 Size: 426 4732 3306 Fragments arranged by size: 4732 3306 426 AleI CACnn'nnGTG Cuts at: 1166 5708 1166 Size: 4542 3922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4665 4856 1184 Size: 3481 191 4792 Fragments arranged by size: 4792 3481 191 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3401 3433 3401 Size: 32 8432 AlwNI CAG_nnn'CTG Cuts at: 2632 2674 3196 3394 4063 4666 4993 5044 6207 Size: 42 522 198 669 603 327 51 1163 Cuts at: 6207 7779 2632 Size: 1572 3317 Fragments arranged by size: 3317 1572 1163 669 603 522 327 198 51 42 ApaI G_GGCC'C Cuts at: 3765 3765 Size: 8464 ApaLI G'TGCA_C Cuts at: 3004 3254 6521 7677 3004 Size: 250 3267 1156 3791 Fragments arranged by size: 3791 3267 1156 250 ApoI r'AATT_y Cuts at: 1493 2125 2374 2492 3785 3823 4625 5625 5773 Size: 632 249 118 1293 38 802 1000 148 Cuts at: 5773 5999 6151 8260 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 1000 802 632 249 226 152 148 118 38 AseI AT'TA_AT Cuts at: 160 1032 7018 160 Size: 872 5986 1606 Fragments arranged by size: 5986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8464 AvaI C'yCGr_G Cuts at: 1870 1943 2274 3127 5789 1870 Size: 73 331 853 2662 4545 Fragments arranged by size: 4545 2662 853 331 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4762 4795 4762 Size: 33 8431 BamHI G'GATC_C Cuts at: 3909 3942 4673 5301 5794 6161 3909 Size: 33 731 628 493 367 6212 Fragments arranged by size: 6212 731 628 493 367 33 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 4446 5785 7240 618 Size: 325 217 627 640 2019 1339 1455 1842 Fragments arranged by size: 2019 1842 1455 1339 640 627 325 217 BanII G_rGCy'C Cuts at: 1391 2097 2715 3128 3585 3765 4545 5783 1391 Size: 706 618 413 457 180 780 1238 4072 Fragments arranged by size: 4072 1238 780 706 618 457 413 180 BbeI G_GCGC'C Cuts at: 2431 2431 Size: 8464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 3155 3557 4525 4859 4979 928 Size: 971 252 1004 402 968 334 120 4413 Fragments arranged by size: 4413 1004 971 968 402 334 252 120 BbvCI CC'TCA_GC Cuts at: 5211 5211 Size: 8464 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2985 3019 3157 3191 4176 4210 6655 Size: 34 1048 34 138 34 985 34 2445 Cuts at: 6655 6689 1903 Size: 34 3678 Fragments arranged by size: 3678 2445 1048 985 138 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4928 6356 7566 4928 Size: 1428 1210 5826 Fragments arranged by size: 5826 1428 1210 BclI T'GATC_A Cuts at: 1548 2478 3375 4305 5493 1548 Size: 930 897 930 1188 4519 Fragments arranged by size: 4519 1188 930 930 897 BfrBI ATG'CAT Cuts at: 2156 5362 2156 Size: 3206 5258 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3493 7075 Size: 107 122 71 631 605 337 1484 3582 Cuts at: 7075 136 Size: 1525 Fragments arranged by size: 3582 1525 1484 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 4878 8459 4878 Size: 3581 4883 BlpI GC'TnA_GC Cuts at: 4439 5881 4439 Size: 1442 7022 Bme1580I G_kGCm'C Cuts at: 1790 3008 3258 3765 5705 6525 7681 1790 Size: 1218 250 507 1940 820 1156 2573 Fragments arranged by size: 2573 1940 1218 1156 820 507 250 BmgBI CAC'GTC Cuts at: 1318 3003 4531 1318 Size: 1685 1528 5251 Fragments arranged by size: 5251 1685 1528 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 2769 3574 Size: 416 53 83 399 24 1078 274 805 Cuts at: 3574 5210 5488 6195 7153 442 Size: 1636 278 707 958 1753 Fragments arranged by size: 1753 1636 1078 958 805 707 416 399 278 274 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4124 1056 Size: 3068 5396 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2595 4924 2595 Size: 2329 6135 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2075 2243 2281 2369 2894 3652 3908 Size: 806 30 168 38 88 525 758 256 Cuts at: 3908 3983 4684 4943 7124 1239 Size: 75 701 259 2181 2579 Fragments arranged by size: 2579 2181 806 758 701 525 259 256 168 88 75 38 30 Bpu10I CC'TnA_GC Cuts at: 5211 5211 Size: 8464 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3763 3776 6589 7454 7752 3763 Size: 13 2813 865 298 4475 Fragments arranged by size: 4475 2813 865 298 13 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3959 7127 882 Size: 617 2460 3168 2219 Fragments arranged by size: 3168 2460 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2591 3226 3994 4849 6255 493 Size: 711 1387 635 768 855 1406 2702 Fragments arranged by size: 2702 1406 1387 855 768 711 635 BsaBI GATnn'nnATC Cuts at: 3056 8458 3056 Size: 5402 3062 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2428 2771 4470 6271 Size: 53 83 186 936 895 343 1699 1801 Cuts at: 6271 6653 7288 275 Size: 382 635 1451 Fragments arranged by size: 1801 1699 1451 936 895 635 382 343 186 83 53 BsaWI w'CCGG_w Cuts at: 4590 4719 5304 6897 7295 7569 7716 8127 4590 Size: 129 585 1593 398 274 147 411 4927 Fragments arranged by size: 4927 1593 585 411 398 274 147 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2824 2854 3257 3287 3401 Size: 30 165 30 1421 30 403 30 114 Cuts at: 3401 3431 1178 Size: 30 6211 Fragments arranged by size: 6211 1421 403 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2240 2278 2461 3356 4265 4811 1186 Size: 886 168 38 183 895 909 546 4839 Fragments arranged by size: 4839 909 895 886 546 183 168 38 BseYI C'CCAG_C Cuts at: 3595 4311 5345 7667 8163 3595 Size: 716 1034 2322 496 3896 Fragments arranged by size: 3896 2322 1034 716 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2560 4289 4511 5057 1163 Size: 573 824 1729 222 546 4570 Fragments arranged by size: 4570 1729 824 573 546 222 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5914 6675 7703 1540 Size: 275 186 3913 761 1028 2301 Fragments arranged by size: 3913 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2715 3008 3258 3999 4545 6525 6610 7681 2715 Size: 293 250 741 546 1980 85 1071 3498 Fragments arranged by size: 3498 1980 1071 741 546 293 250 85 BsmI GAATG_Cn' Cuts at: 4199 5421 5975 6068 4199 Size: 1222 554 93 6595 Fragments arranged by size: 6595 1222 554 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3563 4238 4324 3563 Size: 675 86 7703 Fragments arranged by size: 7703 675 86 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2715 3008 3128 3258 3585 3765 Size: 399 307 618 293 120 130 327 180 Cuts at: 3765 3999 4545 5705 5783 6525 6610 7681 1391 Size: 234 546 1160 78 742 85 1071 2174 Fragments arranged by size: 2174 1160 1071 742 618 546 399 327 307 293 234 180 130 120 85 78 BspEI T'CCGG_A Cuts at: 5304 8127 5304 Size: 2823 5641 BspHI T'CATG_A Cuts at: 6353 8083 6353 Size: 1730 6734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3379 5001 5076 5250 5819 844 Size: 2535 1622 75 174 569 3489 Fragments arranged by size: 3489 2535 1622 569 174 75 BsrBI CCG'CTC Cuts at: 1394 1823 2579 3627 6351 1394 Size: 429 756 1048 2724 3507 Fragments arranged by size: 3507 2724 1048 756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3711 5356 6959 7133 65 Size: 1183 2463 1645 1603 174 1396 Fragments arranged by size: 2463 1645 1603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4443 4590 4758 7108 7295 1700 Size: 110 2633 147 168 2350 187 2869 Fragments arranged by size: 2869 2633 2350 187 168 147 110 BsrGI T'GTAC_A Cuts at: 96 2253 4014 96 Size: 2157 1761 4546 Fragments arranged by size: 4546 2157 1761 BssHII G'CGCG_C Cuts at: 1925 3095 3219 1925 Size: 1170 124 7170 Fragments arranged by size: 7170 1170 124 BssSI C'ACGA_G Cuts at: 4898 5438 6524 7536 4898 Size: 540 1086 1012 5826 Fragments arranged by size: 5826 1086 1012 540 BstAPI GCAn_nnn'nTGC Cuts at: 794 3196 794 Size: 2402 6062 BstBI TT'CG_AA Cuts at: 7268 7268 Size: 8464 BstEII G'GTnAC_C Cuts at: 1184 3472 1184 Size: 2288 6176 BstXI CCAn_nnnn'nTGG Cuts at: 2644 3772 2644 Size: 1128 7336 BstYI r'GATC_y Cuts at: 1972 2602 3057 3909 3942 4673 4878 5301 5794 Size: 630 455 852 33 731 205 423 493 Cuts at: 5794 6161 6546 6563 8015 8101 8113 8459 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 852 731 630 493 455 423 385 367 346 205 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2355 3479 2355 Size: 1124 7340 BtgI C'CryG_G Cuts at: 513 1065 2004 2337 3657 3897 4785 5427 513 Size: 552 939 333 1320 240 888 642 3550 Fragments arranged by size: 3550 1320 939 888 642 552 333 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2155 5209 487 Size: 683 56 929 3054 3742 Fragments arranged by size: 3742 3054 929 683 56 BtsI GCAGTG_nn' Cuts at: 2548 3218 3452 3851 5462 5980 6774 6794 2548 Size: 670 234 399 1611 518 794 20 4218 Fragments arranged by size: 4218 1611 794 670 518 399 234 20 ClaI AT'CG_AT Cuts at: 1554 3980 1554 Size: 2426 6038 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8429 DraI TTT'AAA Cuts at: 5768 5768 Size: 8464 DraIII CAC_nnn'GTG Cuts at: 4498 4498 Size: 8464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7471 809 Size: 507 6155 1802 Fragments arranged by size: 6155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 5911 6800 Size: 54 1425 50 275 18 816 3265 889 Cuts at: 6800 8429 8 Size: 1629 43 Fragments arranged by size: 3265 1629 1425 889 816 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5911 1537 Size: 275 4099 4090 Fragments arranged by size: 4099 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2703 3179 3297 5024 6394 1353 Size: 1350 476 118 1727 1370 3423 Fragments arranged by size: 3423 1727 1370 1350 476 118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7051 7425 7571 387 Size: 1064 138 5462 374 146 1280 Fragments arranged by size: 5462 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2713 4543 2713 Size: 1830 6634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2075 2243 2281 2369 2546 2894 Size: 393 413 30 168 38 88 177 348 Cuts at: 2894 3321 3652 3908 3983 4274 4684 4826 4943 Size: 427 331 256 75 291 410 142 117 Cuts at: 4943 4964 4991 5702 6527 7124 7911 8179 1239 Size: 21 27 711 825 597 787 268 1524 Fragments arranged by size: 1524 825 787 711 597 427 413 410 393 348 331 291 268 256 177 168 142 117 88 75 38 30 27 21 EcoNI CCTnn'n_nnAGG Cuts at: 2054 3359 4513 4997 2054 Size: 1305 1154 484 5521 Fragments arranged by size: 5521 1305 1154 484 EcoO109I rG'GnC_Cy Cuts at: 3193 3616 3662 3761 4733 5205 5239 3193 Size: 423 46 99 972 472 34 6418 Fragments arranged by size: 6418 972 472 423 99 46 34 EcoRI G'AATT_C Cuts at: 5625 5773 6151 5625 Size: 148 378 7938 Fragments arranged by size: 7938 378 148 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3646 3757 3646 Size: 111 8353 FspI TGC'GCA Cuts at: 6970 6970 Size: 8464 HaeII r_GCGC'y Cuts at: 1678 2431 2973 3188 3300 4029 4508 5260 7611 Size: 753 542 215 112 729 479 752 2351 Cuts at: 7611 1678 Size: 2531 Fragments arranged by size: 2531 2351 753 752 729 542 479 215 112 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2307 2339 2447 Size: 32 135 32 449 32 254 32 108 Cuts at: 2447 2479 2594 2626 3257 3289 4200 4232 4367 Size: 32 115 32 631 32 911 32 135 Cuts at: 4367 4399 7105 7137 7179 7211 1373 Size: 32 2706 32 42 32 2626 Fragments arranged by size: 2706 2626 911 631 449 254 135 135 115 108 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5808 6054 669 Size: 988 281 3870 246 3079 Fragments arranged by size: 3870 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3248 748 Size: 2500 5964 HpaI GTT'AAC Cuts at: 6054 6054 Size: 8464 KasI G'GCGC_C Cuts at: 2427 2427 Size: 8464 KpnI G_GTAC'C Cuts at: 4450 5789 4450 Size: 1339 7125 MfeI C'AATT_G Cuts at: 6063 6063 Size: 8464 MluI A'CGCG_T Cuts at: 8340 8340 Size: 8464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2064 2835 3300 4389 7578 7762 1305 Size: 759 771 465 1089 3189 184 2007 Fragments arranged by size: 3189 2007 1089 771 759 465 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 10 Size: 54 1425 343 816 5826 Fragments arranged by size: 5826 1425 816 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 3926 5363 5426 5708 6422 6781 Size: 648 1335 1425 1437 63 282 714 359 Cuts at: 6781 6940 518 Size: 159 2042 Fragments arranged by size: 2042 1437 1425 1335 714 648 359 282 159 63 MspA1I CmG'CkG Cuts at: 1606 3202 3490 3865 4942 5173 5518 5880 6557 Size: 1596 288 375 1077 231 345 362 677 Cuts at: 6557 7705 7950 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1596 1148 1077 677 375 362 345 288 245 231 NaeI GCC'GGC Cuts at: 4760 4760 Size: 8464 NarI GG'CG_CC Cuts at: 2428 2428 Size: 8464 NcoI C'CATG_G Cuts at: 513 1065 2004 3897 513 Size: 552 939 1893 5080 Fragments arranged by size: 5080 1893 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8464 NgoMIV G'CCGG_C Cuts at: 4758 4758 Size: 8464 NheI G'CTAG_C Cuts at: 1052 4120 1052 Size: 3068 5396 NotI GC'GGCC_GC Cuts at: 5911 5911 Size: 8464 NsiI A_TGCA'T Cuts at: 2158 5364 2158 Size: 3206 5258 NspI r_CATG'y Cuts at: 1132 1229 2506 2791 2938 3076 4268 5366 5720 Size: 97 1277 285 147 138 1192 1098 354 Cuts at: 5720 5822 7367 1132 Size: 102 1545 2229 Fragments arranged by size: 2229 1545 1277 1192 1098 354 285 147 138 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8464 PciI A'CATG_T Cuts at: 2502 7363 2502 Size: 4861 3603 PflMI CCAn_nnn'nTGG Cuts at: 1108 3961 1108 Size: 2853 5611 PfoI T'CCnGG_A Cuts at: 2052 2616 3242 3885 3960 4479 4920 5667 2052 Size: 564 626 643 75 519 441 747 4849 Fragments arranged by size: 4849 747 643 626 564 519 441 75 PmeI GTTT'AAAC Cuts at: 5768 5768 Size: 8464 PmlI CAC'GTG Cuts at: 3226 3226 Size: 8464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 3401 3433 6533 6565 8046 8078 2543 Size: 32 826 32 3100 32 1481 32 2929 Fragments arranged by size: 3100 2929 1481 826 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3616 3662 4733 5205 5239 3616 Size: 46 1071 472 34 6841 Fragments arranged by size: 6841 1071 472 46 34 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8464 PsiI TTA'TAA Cuts at: 4146 6034 4146 Size: 1888 6576 PspOMI G'GGCC_C Cuts at: 3761 3761 Size: 8464 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4679 4711 4679 Size: 32 8432 PstI C_TGCA'G Cuts at: 830 2679 3192 3201 3390 3459 3540 3843 4845 Size: 1849 513 9 189 69 81 303 1002 Cuts at: 4845 5816 830 Size: 971 3478 Fragments arranged by size: 3478 1849 1002 971 513 303 189 81 69 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8464 PvuII CAG'CTG Cuts at: 3202 3865 4942 3202 Size: 663 1077 6724 Fragments arranged by size: 6724 1077 663 SacI G_AGCT'C Cuts at: 2715 4545 2715 Size: 1830 6634 SalI G'TCGA_C Cuts at: 1936 5806 1936 Size: 3870 4594 SanDI GG'GwC_CC Cuts at: 3662 5205 3662 Size: 1543 6921 SapI GCTCTTCn'nnn_ Cuts at: 2703 3179 2703 Size: 476 7988 SbfI CC_TGCA'GG Cuts at: 3390 3540 5816 3390 Size: 150 2276 6038 Fragments arranged by size: 6038 2276 150 ScaI AGT'ACT Cuts at: 2897 3272 6712 8424 2897 Size: 375 3440 1712 2937 Fragments arranged by size: 3440 2937 1712 375 SexAI A'CCwGG_T Cuts at: 4043 4043 Size: 8464 SfcI C'TryA_G Cuts at: 826 1045 2675 3100 3188 3197 3386 3455 3536 Size: 219 1630 425 88 9 189 69 81 Cuts at: 3536 3839 4841 5812 6947 7628 7819 826 Size: 303 1002 971 1135 681 191 1471 Fragments arranged by size: 1630 1471 1135 1002 971 681 425 303 219 191 189 88 81 69 9 SfoI GGC'GCC Cuts at: 2429 2429 Size: 8464 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8464 SmaI CCC'GGG Cuts at: 1872 2276 5791 1872 Size: 404 3515 4545 Fragments arranged by size: 4545 3515 404 SmlI C'TyrA_G Cuts at: 820 1017 1943 3742 3755 6568 7469 7731 820 Size: 197 926 1799 13 2813 901 262 1553 Fragments arranged by size: 2813 1799 1553 926 901 262 197 13 SnaBI TAC'GTA Cuts at: 493 2591 6255 493 Size: 2098 3664 2702 Fragments arranged by size: 3664 2702 2098 SpeI A'CTAG_T Cuts at: 152 152 Size: 8464 SphI G_CATG'C Cuts at: 1132 5822 1132 Size: 4690 3774 SspI AAT'ATT Cuts at: 5 52 6388 5 Size: 47 6336 2081 Fragments arranged by size: 6336 2081 47 StuI AGG'CCT Cuts at: 1517 3384 1517 Size: 1867 6597 StyI C'CwwG_G Cuts at: 513 1065 2004 3897 3969 4677 5903 513 Size: 552 939 1893 72 708 1226 3074 Fragments arranged by size: 3074 1893 1226 939 708 552 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4325 6491 6508 6661 6846 7291 325 Size: 1255 2745 2166 17 153 185 445 1498 Fragments arranged by size: 2745 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2382 2895 Size: 274 80 33 51 1443 276 129 513 Cuts at: 2895 3030 3270 4014 6710 8422 96 Size: 135 240 744 2696 1712 138 Fragments arranged by size: 2696 1712 1443 744 513 276 274 240 138 135 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2598 2630 8424 8456 2598 Size: 32 5794 32 2606 Fragments arranged by size: 5794 2606 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8464 XbaI T'CTAG_A Cuts at: 5800 5800 Size: 8464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4393 5570 4393 Size: 1177 7287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8464 XmaI C'CCGG_G Cuts at: 1870 2274 5789 1870 Size: 404 3515 4545 Fragments arranged by size: 4545 3515 404 XmnI GAAnn'nnTTC Cuts at: 2456 3786 6593 2456 Size: 1330 2807 4327 Fragments arranged by size: 4327 2807 1330 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6272 276 Size: 53 83 186 936 4738 2468 Fragments arranged by size: 4738 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI NruI PacI PspXI RsrII SacII SfiI SrfI SwaI