Restriction map for pFN21AA0068
 (Linear) (Six-base) MAPSORT of: KIAA0068.seq  Check: 8940  from: 1  to: 3759



KIAA0068.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1374    3759 
   Size:       1374    2385
 
AccI GT'mk_AC

Cuts at:      0    3410    3759 
   Size:       3410     349
 
Acc65I G'GTAC_C

Cuts at:      0    2441    3759 
   Size:       2441    1318
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541    1316    2269    2821    2959    2986    3697    3759 
   Size:        541     775     953     552     138      27     711      62

  Fragments arranged by size: 

                 953     775     711     552     541     138      62      27
            
 
AfeI AGC'GCT

Cuts at:      0    1293    3759 
   Size:       1293    2466
 
AflIII A'CryG_T

Cuts at:      0     497    2492    3759 
   Size:        497    1995    1267

  Fragments arranged by size: 

                1995    1267     497
 
AgeI A'CCGG_T

Cuts at:      0    2585    3759 
   Size:       2585    1174
 
AhdI GACnn_n'nnGTC

Cuts at:      0      30     456    3759 
   Size:         30     426    3303

  Fragments arranged by size: 

                3303     426      30
 
AleI CACnn'nnGTG

Cuts at:      0    3703    3759 
   Size:       3703      56
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2660    2851    3759 
   Size:       2660     191     908

  Fragments arranged by size: 

                2660     908     191
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1396    1428    3759 
   Size:       1396      32    2331

  Fragments arranged by size: 

                2331    1396      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     627     669    1191    1389    2058    2661    2988    3039 
   Size:        627      42     522     198     669     603     327      51

Cuts at:   3039    3759 
   Size:        720

  Fragments arranged by size: 

                 720     669     627     603     522     327     198      51
                  42
 
ApaI G_GGCC'C

Cuts at:      0    1760    3759 
   Size:       1760    1999
 
ApaLI G'TGCA_C

Cuts at:      0     999    1249    3759 
   Size:        999     250    2510

  Fragments arranged by size: 

                2510     999     250
 
ApoI r'AATT_y

Cuts at:      0     120     369     487    1780    1818    2620    3620    3759 
   Size:        120     249     118    1293      38     802    1000     139

  Fragments arranged by size: 

                1293    1000     802     249     139     120     118      38
            
 
AvaI C'yCGr_G

Cuts at:      0     269    1122    3759 
   Size:        269     853    2637

  Fragments arranged by size: 

                2637     853     269
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2757    2790    3759 
   Size:       2757      33     969

  Fragments arranged by size: 

                2757     969      33
 
BamHI G'GATC_C

Cuts at:      0    1904    1937    2668    3296    3759 
   Size:       1904      33     731     628     463

  Fragments arranged by size: 

                1904     731     628     463      33
 
BanI G'GyrC_C

Cuts at:      0     422    2441    3759 
   Size:        422    2019    1318

  Fragments arranged by size: 

                2019    1318     422
 
BanII G_rGCy'C

Cuts at:      0      92     710    1123    1580    1760    2540    3759 
   Size:         92     618     413     457     180     780    1219

  Fragments arranged by size: 

                1219     780     618     457     413     180      92
 
BbeI G_GCGC'C

Cuts at:      0     426    3759 
   Size:        426    3333
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146    1150    1552    2520    2854    2974    3759 
   Size:        146    1004     402     968     334     120     785

  Fragments arranged by size: 

                1004     968     785     402     334     146     120
 
BbvCI CC'TCA_GC

Cuts at:      0    3206    3759 
   Size:       3206     553
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     980    1014    1152    1186    2171    2205    3759 
   Size:        980      34     138      34     985      34    1554

  Fragments arranged by size: 

                1554     985     980     138      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2923    3759 
   Size:       2923     836
 
BclI T'GATC_A

Cuts at:      0     473    1370    2300    3488    3759 
   Size:        473     897     930    1188     271

  Fragments arranged by size: 

                1188     930     897     473     271
 
BfrBI ATG'CAT

Cuts at:      0     151    3357    3759 
   Size:        151    3206     402

  Fragments arranged by size: 

                3206     402     151
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1488    3759 
   Size:       1488    2271
 
BglII A'GATC_T

Cuts at:      0    2873    3759 
   Size:       2873     886
 
BlpI GC'TnA_GC

Cuts at:      0    2434    3759 
   Size:       2434    1325
 
Bme1580I G_kGCm'C

Cuts at:      0    1003    1253    1760    3700    3759 
   Size:       1003     250     507    1940      59

  Fragments arranged by size: 

                1940    1003     507     250      59
 
BmgBI CAC'GTC

Cuts at:      0     998    2526    3759 
   Size:        998    1528    1233

  Fragments arranged by size: 

                1528    1233     998
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     490     764    1569    3205    3483    3759 
   Size:        490     274     805    1636     278     276

  Fragments arranged by size: 

                1636     805     490     278     276     274
 
BmtI G_CTAG'C

Cuts at:      0    2119    3759 
   Size:       2119    1640
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     590    2919    3759 
   Size:        590    2329     840

  Fragments arranged by size: 

                2329     840     590
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      70     238     276     364     889    1647    1903 
   Size:         40      30     168      38      88     525     758     256

Cuts at:   1903    1978    2679    2938    3759 
   Size:         75     701     259     821

  Fragments arranged by size: 

                 821     758     701     525     259     256     168      88
                  75      40      38      30
 
Bpu10I CC'TnA_GC

Cuts at:      0    3206    3759 
   Size:       3206     553
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1758    1771    3759 
   Size:       1758      13    1988

  Fragments arranged by size: 

                1988    1758      13
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1954    3759 
   Size:       1954    1805
 
BsaAI yAC'GTr

Cuts at:      0     586    1221    1989    2844    3759 
   Size:        586     635     768     855     915

  Fragments arranged by size: 

                 915     855     768     635     586
 
BsaBI GATnn'nnATC

Cuts at:      0    1051    3759 
   Size:       1051    2708
 
BsaHI Gr'CG_yC

Cuts at:      0     423     766    2465    3759 
   Size:        423     343    1699    1294

  Fragments arranged by size: 

                1699    1294     423     343
 
BsaWI w'CCGG_w

Cuts at:      0    2585    2714    3299    3759 
   Size:       2585     129     585     460

  Fragments arranged by size: 

                2585     585     460     129
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     819     849    1252    1282    1396    1426    3759 
   Size:        819      30     403      30     114      30    2333

  Fragments arranged by size: 

                2333     819     403     114      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     235     273     456    1351    2260    2806    3759 
   Size:         67     168      38     183     895     909     546     953

  Fragments arranged by size: 

                 953     909     895     546     183     168      67      38
            
 
BseYI C'CCAG_C

Cuts at:      0    1590    2306    3340    3759 
   Size:       1590     716    1034     419

  Fragments arranged by size: 

                1590    1034     716     419
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    2284    2506    3052    3759 
   Size:        555    1729     222     546     707

  Fragments arranged by size: 

                1729     707     555     546     222
 
BsiHKAI G_wGCw'C

Cuts at:      0     710    1003    1253    1994    2540    3759 
   Size:        710     293     250     741     546    1219

  Fragments arranged by size: 

                1219     741     710     546     293     250
 
BsmI GAATG_Cn'

Cuts at:      0    2194    3416    3759 
   Size:       2194    1222     343

  Fragments arranged by size: 

                2194    1222     343
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1558    2233    2319    3759 
   Size:       1558     675      86    1440

  Fragments arranged by size: 

                1558    1440     675      86
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     710    1003    1123    1253    1580    1760    1994 
   Size:         92     618     293     120     130     327     180     234

Cuts at:   1994    2540    3700    3759 
   Size:        546    1160      59

  Fragments arranged by size: 

                1160     618     546     327     293     234     180     130
                 120      92      59
 
BspEI T'CCGG_A

Cuts at:      0    3299    3759 
   Size:       3299     460
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1374    2996    3071    3245    3759 
   Size:       1374    1622      75     174     514

  Fragments arranged by size: 

                1622    1374     514     174      75
 
BsrBI CCG'CTC

Cuts at:      0     574    1622    3759 
   Size:        574    1048    2137

  Fragments arranged by size: 

                2137    1048     574
 
BsrDI GCAATG_nn'

Cuts at:      0    1706    3351    3759 
   Size:       1706    1645     408

  Fragments arranged by size: 

                1706    1645     408
 
BsrFI r'CCGG_y

Cuts at:      0    2438    2585    2753    3759 
   Size:       2438     147     168    1006

  Fragments arranged by size: 

                2438    1006     168     147
 
BsrGI T'GTAC_A

Cuts at:      0     248    2009    3759 
   Size:        248    1761    1750

  Fragments arranged by size: 

                1761    1750     248
 
BssHII G'CGCG_C

Cuts at:      0    1090    1214    3759 
   Size:       1090     124    2545

  Fragments arranged by size: 

                2545    1090     124
 
BssSI C'ACGA_G

Cuts at:      0    2893    3433    3759 
   Size:       2893     540     326

  Fragments arranged by size: 

                2893     540     326
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1191    3759 
   Size:       1191    2568
 
BstEII G'GTnAC_C

Cuts at:      0    1467    3759 
   Size:       1467    2292
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     639    1767    3759 
   Size:        639    1128    1992

  Fragments arranged by size: 

                1992    1128     639
 
BstYI r'GATC_y

Cuts at:      0     597    1052    1904    1937    2668    2873    3296    3759 
   Size:        597     455     852      33     731     205     423     463

  Fragments arranged by size: 

                 852     731     597     463     455     423     205      33
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     350    1474    3759 
   Size:        350    1124    2285

  Fragments arranged by size: 

                2285    1124     350
 
BtgI C'CryG_G

Cuts at:      0     332    1652    1892    2780    3422    3759 
   Size:        332    1320     240     888     642     337

  Fragments arranged by size: 

                1320     888     642     337     332     240
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     150    3204    3759 
   Size:        150    3054     555

  Fragments arranged by size: 

                3054     555     150
 
BtsI GCAGTG_nn'

Cuts at:      0     543    1213    1447    1846    3457    3759 
   Size:        543     670     234     399    1611     302

  Fragments arranged by size: 

                1611     670     543     399     302     234
 
ClaI AT'CG_AT

Cuts at:      0    1975    3759 
   Size:       1975    1784
 
DraIII CAC_nnn'GTG

Cuts at:      0    2493    3759 
   Size:       2493    1266
 
EaeI y'GGCC_r

Cuts at:      0     641    3759 
   Size:        641    3118
 
EarI CTCTTCn'nnn_

Cuts at:      0     698    1174    1292    3019    3759 
   Size:        698     476     118    1727     740

  Fragments arranged by size: 

                1727     740     698     476     118
 
EcoICRI GAG'CTC

Cuts at:      0     708    2538    3759 
   Size:        708    1830    1221

  Fragments arranged by size: 

                1830    1221     708
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      70     238     276     364     541     889    1316 
   Size:         40      30     168      38      88     177     348     427

Cuts at:   1316    1647    1903    1978    2269    2679    2821    2938    2959 
   Size:        331     256      75     291     410     142     117      21

Cuts at:   2959    2986    3697    3759 
   Size:         27     711      62

  Fragments arranged by size: 

                 711     427     410     348     331     291     256     177
                 168     142     117      88      75      62      40      38
                  30      27      21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      49    1354    2508    2992    3759 
   Size:         49    1305    1154     484     767

  Fragments arranged by size: 

                1305    1154     767     484      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1188    1611    1657    1756    2728    3200    3234    3759 
   Size:       1188     423      46      99     972     472      34     525

  Fragments arranged by size: 

                1188     972     525     472     423      99      46      34
            
 
EcoRI G'AATT_C

Cuts at:      0    3620    3759 
   Size:       3620     139
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1641    1752    3759 
   Size:       1641     111    2007

  Fragments arranged by size: 

                2007    1641     111
 
HaeII r_GCGC'y

Cuts at:      0     426     968    1183    1295    2024    2503    3255    3759 
   Size:        426     542     215     112     729     479     752     504

  Fragments arranged by size: 

                 752     729     542     504     479     426     215     112
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     302     334     442     474     589     621 
   Size:         16      32     254      32     108      32     115      32

Cuts at:    621    1252    1284    2195    2227    2362    2394    3759 
   Size:        631      32     911      32     135      32    1365

  Fragments arranged by size: 

                1365     911     631     254     135     115     108      32
                  32      32      32      32      32      32      16
 
HindIII A'AGCT_T

Cuts at:      0    1243    3759 
   Size:       1243    2516
 
KasI G'GCGC_C

Cuts at:      0     422    3759 
   Size:        422    3337
 
KpnI G_GTAC'C

Cuts at:      0    2445    3759 
   Size:       2445    1314
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     830    1295    2384    3759 
   Size:         59     771     465    1089    1375

  Fragments arranged by size: 

                1375    1089     771     465      59
 
MscI TGG'CCA

Cuts at:      0     643    3759 
   Size:        643    3116
 
MslI CAynn'nnrTG

Cuts at:      0     496    1921    3358    3421    3703    3759 
   Size:        496    1425    1437      63     282      56

  Fragments arranged by size: 

                1437    1425     496     282      63      56
 
MspA1I CmG'CkG

Cuts at:      0    1197    1485    1860    2937    3168    3513    3759 
   Size:       1197     288     375    1077     231     345     246

  Fragments arranged by size: 

                1197    1077     375     345     288     246     231
 
NaeI GCC'GGC

Cuts at:      0    2755    3759 
   Size:       2755    1004
 
NarI GG'CG_CC

Cuts at:      0     423    3759 
   Size:        423    3336
 
NcoI C'CATG_G

Cuts at:      0    1892    3759 
   Size:       1892    1867
 
NgoMIV G'CCGG_C

Cuts at:      0    2753    3759 
   Size:       2753    1006
 
NheI G'CTAG_C

Cuts at:      0    2115    3759 
   Size:       2115    1644
 
NsiI A_TGCA'T

Cuts at:      0     153    3359    3759 
   Size:        153    3206     400

  Fragments arranged by size: 

                3206     400     153
 
NspI r_CATG'y

Cuts at:      0     501     786     933    1071    2263    3361    3715    3759 
   Size:        501     285     147     138    1192    1098     354      44

  Fragments arranged by size: 

                1192    1098     501     354     285     147     138      44
            
 
PciI A'CATG_T

Cuts at:      0     497    3759 
   Size:        497    3262
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1956    3759 
   Size:       1956    1803
 
PfoI T'CCnGG_A

Cuts at:      0      47     611    1237    1880    1955    2474    2915    3662 
   Size:         47     564     626     643      75     519     441     747

Cuts at:   3662    3759 
   Size:         97

  Fragments arranged by size: 

                 747     643     626     564     519     441      97      75
                  47
 
PmlI CAC'GTG

Cuts at:      0    1221    3759 
   Size:       1221    2538
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     538     570    1396    1428    3759 
   Size:        538      32     826      32    2331

  Fragments arranged by size: 

                2331     826     538      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1611    1657    2728    3200    3234    3759 
   Size:       1611      46    1071     472      34     525

  Fragments arranged by size: 

                1611    1071     525     472      46      34
 
PsiI TTA'TAA

Cuts at:      0    2141    3759 
   Size:       2141    1618
 
PspOMI G'GGCC_C

Cuts at:      0    1756    3759 
   Size:       1756    2003
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2674    2706    3759 
   Size:       2674      32    1053

  Fragments arranged by size: 

                2674    1053      32
 
PstI C_TGCA'G

Cuts at:      0     674    1187    1196    1385    1454    1535    1838    2840 
   Size:        674     513       9     189      69      81     303    1002

Cuts at:   2840    3759 
   Size:        919

  Fragments arranged by size: 

                1002     919     674     513     303     189      81      69
                   9
 
PvuII CAG'CTG

Cuts at:      0    1197    1860    2937    3759 
   Size:       1197     663    1077     822

  Fragments arranged by size: 

                1197    1077     822     663
 
SacI G_AGCT'C

Cuts at:      0     710    2540    3759 
   Size:        710    1830    1219

  Fragments arranged by size: 

                1830    1219     710
 
SanDI GG'GwC_CC

Cuts at:      0    1657    3200    3759 
   Size:       1657    1543     559

  Fragments arranged by size: 

                1657    1543     559
 
SapI GCTCTTCn'nnn_

Cuts at:      0     698    1174    3759 
   Size:        698     476    2585

  Fragments arranged by size: 

                2585     698     476
 
SbfI CC_TGCA'GG

Cuts at:      0    1385    1535    3759 
   Size:       1385     150    2224

  Fragments arranged by size: 

                2224    1385     150
 
ScaI AGT'ACT

Cuts at:      0     892    1267    3759 
   Size:        892     375    2492

  Fragments arranged by size: 

                2492     892     375
 
SexAI A'CCwGG_T

Cuts at:      0    2038    3759 
   Size:       2038    1721
 
SfcI C'TryA_G

Cuts at:      0     670    1095    1183    1192    1381    1450    1531    1834 
   Size:        670     425      88       9     189      69      81     303

Cuts at:   1834    2836    3759 
   Size:       1002     923

  Fragments arranged by size: 

                1002     923     670     425     303     189      88      81
                  69       9
 
SfoI GGC'GCC

Cuts at:      0     424    3759 
   Size:        424    3335
 
SmaI CCC'GGG

Cuts at:      0     271    3759 
   Size:        271    3488
 
SmlI C'TyrA_G

Cuts at:      0    1737    1750    3759 
   Size:       1737      13    2009

  Fragments arranged by size: 

                2009    1737      13
 
SnaBI TAC'GTA

Cuts at:      0     586    3759 
   Size:        586    3173
 
StuI AGG'CCT

Cuts at:      0    1379    3759 
   Size:       1379    2380
 
StyI C'CwwG_G

Cuts at:      0    1892    1964    2672    3759 
   Size:       1892      72     708    1087

  Fragments arranged by size: 

                1892    1087     708      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2320    3759 
   Size:       2320    1439
 
TatI w'GTAC_w

Cuts at:      0     248     377     890    1025    1265    2009    3759 
   Size:        248     129     513     135     240     744    1750

  Fragments arranged by size: 

                1750     744     513     248     240     135     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     593     625    3759 
   Size:        593      32    3134

  Fragments arranged by size: 

                3134     593      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2388    3565    3759 
   Size:       2388    1177     194

  Fragments arranged by size: 

                2388    1177     194
 
XmaI C'CCGG_G

Cuts at:      0     269    3759 
   Size:        269    3490
 
XmnI GAAnn'nnTTC

Cuts at:      0     451    1781    3759 
   Size:        451    1330    1978

  Fragments arranged by size: 

                1978    1330     451
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI   DraIII     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I  HindIII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NsiI     NspI     PciI    PflMI     PfoI
     PmlI     PpiI    PpuMI     PsiI   PspOMI     PsrI     PstI    PvuII
     SacI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SmaI     SmlI    SnaBI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AscI     AseI    AsiSI    AvrII    BsiEI
    BsiWI    BspHI    BstBI  BstZ17I    CspCI     DraI     DrdI     EagI
     EciI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     MfeI
     MluI     NdeI     NotI     NruI     PacI     PasI     PmeI    PshAI
    PspXI     PvuI    RsrII    SacII     SalI     SfiI     SgfI    SgrAI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     ZraI
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