Restriction map for
pFN21AA0066
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbeI BcgI BciVI
BfrI BlnI BsaHI BseAI BsiEI
BsiWI BsmBI BsmFI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FauI FseI HaeII Hin1I HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
NarI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 2837 CGCG CG^CG
AgeI 2647 ACCGGT A^CCGGT
AocI 1822 CCTAAGG CC^T[ACGT]AGG
AvaI 2111 CCCGGG C^[CT]CG[GA]G
AviII 2822 TGCGCA TGC^GCA
BamHI 906 GGATCC G^GATCC
BbrPI 1586 CACGTG CAC^GTG
BclI 1397 TGATCA T^GATCA
BglI 2701 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2227 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2227 GCTAAGC GC^T[ACGT]AGC
BsaBI 901 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1658 GAGACC GGTCTC(1/5)
BsaWI 2647 ACCGGT [AT]^CCGG[AT]
BsoBI 2111 CCCGGG C^[CT]CG[GA]G
BstUI 2837 CGCG CG^CG
Bsu36I 1822 CCTAAGG CC^T[ACGT]AGG
CelII 2227 GCTAAGC GC^T[ACGT]AGC
DraI 994 TTTAAA TTT^AAA
DraIII 401 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1410 CCATGG C^C[GA][CT]GG
EaeI 2805 TGGCCG [CT]^GGCC[GA]
Eco81I 1822 CCTAAGG CC^T[ACGT]AGG
EcoNI 1437 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2812 GAATTC G^AATTC
EcoRV 628 GATATC GAT^ATC
FbaI 1397 TGATCA T^GATCA
FspI 2822 TGCGCA TGC^GCA
HgaI 409 GACGC GACGC(5/10)
HincII 1017 GTCAAC GT[CT]^[GA]AC
HindII 1017 GTCAAC GT[CT]^[GA]AC
MamI 901 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2837 CGCG CG^CG
NaeI 1107 GCCGGC GCC^GGC
NcoI 1410 CCATGG C^CATGG
NgoMIV 1107 GCCGGC G^CCGGC
PflMI 1267 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2647 ACCGGT A^CCGGT
PmaCI 1586 CACGTG CAC^GTG
PmlI 1586 CACGTG CAC^GTG
PvuII 2360 CAGCTG CAG^CTG
SacI 2534 GAGCTC GAGCT^C
SapI 1954 GAAGAGC GCTCTTC(1/4)
SgrAI 2646 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2111 CCCGGG CCC^GGG
SmlI 2368 CTCAAG C^T[CT][GA]AG
SphI 2018 GCATGC GCATG^C
Van91I 1267 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2194 CCAAGCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2111 CCCGGG C^CCGGG
XmaI 2111 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BbsI 1318 GAAGAC GAAGAC(2/6)
2473 GAAGAC
BbvCI 733 CCTCAGC CCTCAGC(-5/-2)
2866 CCTCAGC
BpuAI 1318 GAAGAC GAAGAC(2/6)
2473 GAAGAC
BsaAI 371 TACGTG [CT]AC^GT[GA]
1586 CACGTG
BsmI 1759 GAATGC GAATGC(1/-1)
2697 GAATGC
Bsp1407I 237 TGTACA T^GTACA
2876 TGTACA
BspMI 2316 ACCTGC ACCTGC(4/8)
2911 GCAGGT
BsrFI 1107 GCCGGC [GA]^CCGG[CT]
2647 ACCGGT
BsrGI 237 TGTACA T^GTACA
2876 TGTACA
BstAPI 303 GCAAGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2320 GCAGACCTTGC
BstBI 568 TTCGAA TT^CGAA
941 TTCGAA
CfoI 2823 GCGC GCG^C
2838 GCGC
Cfr10I 1107 GCCGGC [GA]^CCGG[CT]
2647 ACCGGT
DraII 546 AGGTCCT [GA]G^G[ACGT]CC[CT]
1818 AGGACCT
EcoO109I 546 AGGTCCT [GA]G^G[ACGT]CC[CT]
1818 AGGACCT
EcoT14I 464 CCTTGG C^C[AT][AT]GG
1410 CCATGG
HhaI 2823 GCGC GCG^C
2838 GCGC
HinP1I 2823 GCGC G^CGC
2838 GCGC
MaeII 372 ACGT A^CGT
1587 ACGT
MslI 1180 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2826 CACCACTGTG
MspA1I 2360 CAGCTG C[AC]G^C[GT]G
2869 CAGCGG
NspI 2018 GCATGC [GA]CATG^[CT]
2664 ACATGC
NspV 568 TTCGAA TT^CGAA
941 TTCGAA
PpuMI 546 AGGTCCT [GA]G^G[AT]CC[CT]
1818 AGGACCT
PstI 2318 CTGCAG CTGCA^G
2711 CTGCAG
SfuI 568 TTCGAA TT^CGAA
941 TTCGAA
SspBI 237 TGTACA T^GTACA
2876 TGTACA
StyI 464 CCTTGG C^C[AT][AT]GG
1410 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI