Restriction map for
pFN21AA0066
(Linear) (Six-base) MAPSORT of: KIAA0066.seq Check: 7744 from: 1 to: 2943
KIAA0066.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2902 2943
Size: 2902 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 1796 1985 2390 2654 2943
Size: 1598 198 189 405 264 289
Fragments arranged by size:
1598 405 289 264 198 189
AgeI A'CCGG_T
Cuts at: 0 2647 2943
Size: 2647 296
AleI CACnn'nnGTG
Cuts at: 0 1184 2830 2943
Size: 1184 1646 113
Fragments arranged by size:
1646 1184 113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2173 2848 2943
Size: 2173 675 95
Fragments arranged by size:
2173 675 95
ApoI r'AATT_y
Cuts at: 0 191 503 988 998 1447 1750 1836 2812
Size: 191 312 485 10 449 303 86 976
Cuts at: 2812 2943
Size: 131
Fragments arranged by size:
976 485 449 312 303 191 131 86
10
AvaI C'yCGr_G
Cuts at: 0 2111 2943
Size: 2111 832
BamHI G'GATC_C
Cuts at: 0 906 2943
Size: 906 2037
BanI G'GyrC_C
Cuts at: 0 486 808 1668 2333 2914 2943
Size: 486 322 860 665 581 29
Fragments arranged by size:
860 665 581 486 322 29
BanII G_rGCy'C
Cuts at: 0 20 2004 2538 2755 2943
Size: 20 1984 534 217 188
Fragments arranged by size:
1984 534 217 188 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1325 2480 2943
Size: 1325 1155 463
Fragments arranged by size:
1325 1155 463
BbvCI CC'TCA_GC
Cuts at: 0 734 2867 2943
Size: 734 2133 76
Fragments arranged by size:
2133 734 76
BclI T'GATC_A
Cuts at: 0 1397 2943
Size: 1397 1546
BglI GCCn_nnn'nGGC
Cuts at: 0 2707 2943
Size: 2707 236
BglII A'GATC_T
Cuts at: 0 1518 1944 2595 2943
Size: 1518 426 651 348
Fragments arranged by size:
1518 651 426 348
BlpI GC'TnA_GC
Cuts at: 0 2228 2943
Size: 2228 715
Bme1580I G_kGCm'C
Cuts at: 0 2181 2943
Size: 2181 762
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1918 2943
Size: 1918 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 728 1752 2644 2943
Size: 656 72 1024 892 299
Fragments arranged by size:
1024 892 656 299 72
Bpu10I CC'TnA_GC
Cuts at: 0 734 2867 2943
Size: 734 2133 76
Fragments arranged by size:
2133 734 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2353 2943
Size: 2353 590
BsaI GGTCTCn'nnnn_
Cuts at: 0 1652 2943
Size: 1652 1291
BsaAI yAC'GTr
Cuts at: 0 373 1588 2943
Size: 373 1215 1355
Fragments arranged by size:
1355 1215 373
BsaBI GATnn'nnATC
Cuts at: 0 905 2943
Size: 905 2038
BsaWI w'CCGG_w
Cuts at: 0 2647 2943
Size: 2647 296
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 758 788 2943
Size: 758 30 2155
Fragments arranged by size:
2155 758 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 782 1207 2143 2596 2746 2943
Size: 782 425 936 453 150 197
Fragments arranged by size:
936 782 453 425 197 150
BseYI C'CCAG_C
Cuts at: 0 312 2943
Size: 312 2631
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1334 2278 2672 2943
Size: 383 951 944 394 271
Fragments arranged by size:
951 944 394 383 271
BsiHKAI G_wGCw'C
Cuts at: 0 345 2016 2538 2943
Size: 345 1671 522 405
Fragments arranged by size:
1671 522 405 345
BsmI GAATG_Cn'
Cuts at: 0 1765 2703 2943
Size: 1765 938 240
Fragments arranged by size:
1765 938 240
Bsp1286I G_dGCh'C
Cuts at: 0 20 345 2004 2016 2181 2538 2755 2943
Size: 20 325 1659 12 165 357 217 188
Fragments arranged by size:
1659 357 325 217 188 165 20 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2325 2902 2943
Size: 2325 577 41
Fragments arranged by size:
2325 577 41
BsrDI GCAATG_nn'
Cuts at: 0 345 664 2157 2525 2943
Size: 345 319 1493 368 418
Fragments arranged by size:
1493 418 368 345 319
BsrFI r'CCGG_y
Cuts at: 0 1107 2647 2943
Size: 1107 1540 296
Fragments arranged by size:
1540 1107 296
BsrGI T'GTAC_A
Cuts at: 0 237 2876 2943
Size: 237 2639 67
Fragments arranged by size:
2639 237 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 2326 2943
Size: 309 2017 617
Fragments arranged by size:
2017 617 309
BstBI TT'CG_AA
Cuts at: 0 569 942 2943
Size: 569 373 2001
Fragments arranged by size:
2001 569 373
BstYI r'GATC_y
Cuts at: 0 819 906 1518 1944 2595 2943
Size: 819 87 612 426 651 348
Fragments arranged by size:
819 651 612 426 348 87
Bsu36I CC'TnA_GG
Cuts at: 0 1823 2943
Size: 1823 1120
BtgI C'CryG_G
Cuts at: 0 1410 2943
Size: 1410 1533
BtsI GCAGTG_nn'
Cuts at: 0 53 1154 1879 2943
Size: 53 1101 725 1064
Fragments arranged by size:
1101 1064 725 53
DraI TTT'AAA
Cuts at: 0 996 2943
Size: 996 1947
DraIII CAC_nnn'GTG
Cuts at: 0 406 2943
Size: 406 2537
EaeI y'GGCC_r
Cuts at: 0 2805 2943
Size: 2805 138
EarI CTCTTCn'nnn_
Cuts at: 0 170 1949 2120 2652 2737 2943
Size: 170 1779 171 532 85 206
Fragments arranged by size:
1779 532 206 171 170 85
EcoICRI GAG'CTC
Cuts at: 0 2536 2943
Size: 2536 407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 728 1598 1752 1796 1985 2390 2644
Size: 656 72 870 154 44 189 405 254
Cuts at: 2644 2654 2943
Size: 10 289
Fragments arranged by size:
870 656 405 289 254 189 154 72
44 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1441 2943
Size: 1441 1502
EcoO109I rG'GnC_Cy
Cuts at: 0 547 1819 2943
Size: 547 1272 1124
Fragments arranged by size:
1272 1124 547
EcoRI G'AATT_C
Cuts at: 0 2812 2943
Size: 2812 131
EcoRV GAT'ATC
Cuts at: 0 630 2943
Size: 630 2313
FspI TGC'GCA
Cuts at: 0 2824 2943
Size: 2824 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 636 668 1096 1128 1929 1961
Size: 7 32 597 32 428 32 801 32
Cuts at: 1961 2016 2035 2048 2067 2943
Size: 55 19 13 19 876
Fragments arranged by size:
876 801 597 428 55 32 32 32
32 19 19 13 7
HincII GTy'rAC
Cuts at: 0 1019 2943
Size: 1019 1924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 2943
Size: 756 2187
MslI CAynn'nnrTG
Cuts at: 0 1184 2830 2943
Size: 1184 1646 113
Fragments arranged by size:
1646 1184 113
MspA1I CmG'CkG
Cuts at: 0 2362 2871 2943
Size: 2362 509 72
Fragments arranged by size:
2362 509 72
NaeI GCC'GGC
Cuts at: 0 1109 2943
Size: 1109 1834
NcoI C'CATG_G
Cuts at: 0 1410 2943
Size: 1410 1533
NgoMIV G'CCGG_C
Cuts at: 0 1107 2943
Size: 1107 1836
NspI r_CATG'y
Cuts at: 0 2022 2668 2943
Size: 2022 646 275
Fragments arranged by size:
2022 646 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 1273 2943
Size: 1273 1670
PfoI T'CCnGG_A
Cuts at: 0 1494 1729 2943
Size: 1494 235 1214
Fragments arranged by size:
1494 1214 235
PmlI CAC'GTG
Cuts at: 0 1588 2943
Size: 1588 1355
PpuMI rG'GwC_Cy
Cuts at: 0 547 1819 2943
Size: 547 1272 1124
Fragments arranged by size:
1272 1124 547
PstI C_TGCA'G
Cuts at: 0 2322 2715 2943
Size: 2322 393 228
Fragments arranged by size:
2322 393 228
PvuII CAG'CTG
Cuts at: 0 2362 2943
Size: 2362 581
SacI G_AGCT'C
Cuts at: 0 2538 2943
Size: 2538 405
SapI GCTCTTCn'nnn_
Cuts at: 0 1949 2943
Size: 1949 994
SfcI C'TryA_G
Cuts at: 0 1549 2318 2711 2943
Size: 1549 769 393 232
Fragments arranged by size:
1549 769 393 232
SgrAI Cr'CCGG_yG
Cuts at: 0 2647 2943
Size: 2647 296
SmaI CCC'GGG
Cuts at: 0 2113 2943
Size: 2113 830
SmlI C'TyrA_G
Cuts at: 0 2368 2943
Size: 2368 575
SphI G_CATG'C
Cuts at: 0 2022 2943
Size: 2022 921
StyI C'CwwG_G
Cuts at: 0 464 1410 2943
Size: 464 946 1533
Fragments arranged by size:
1533 946 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 2528 2943
Size: 880 1648 415
Fragments arranged by size:
1648 880 415
TatI w'GTAC_w
Cuts at: 0 237 602 702 2364 2876 2943
Size: 237 365 100 1662 512 67
Fragments arranged by size:
1662 512 365 237 100 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2865 2897 2943
Size: 2865 32 46
Fragments arranged by size:
2865 46 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2201 2943
Size: 2201 742
XmaI C'CCGG_G
Cuts at: 0 2111 2943
Size: 2111 832
XmnI GAAnn'nnTTC
Cuts at: 0 575 1281 1451 2495 2943
Size: 575 706 170 1044 448
Fragments arranged by size:
1044 706 575 448 170
Enzymes that do cut:
AarI AcuI AgeI AleI AlfI ApoI AvaI BamHI
BanI BanII BbsI BbvCI BclI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI Hin4I HincII MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SapI SfcI SgrAI
SmaI SmlI SphI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BfrBI BmgBI BmrI BmtI
BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstEII BstXI BstZ17I BtgZI ClaI CspCI DrdI
EagI EciI FalI FseI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XhoI ZraI