Restriction map for
pFN21ASDF0380
(Linear) (Six-base) MAPSORT of: FLJ00380.seq Check: 8391 from: 1 to: 2316
FLJ00380.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 2316
Size: 796 1520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1116 1499 2316
Size: 31 1085 383 817
Fragments arranged by size:
1085 817 383 31
AlwNI CAG_nnn'CTG
Cuts at: 0 2148 2316
Size: 2148 168
ApaI G_GGCC'C
Cuts at: 0 137 267 2316
Size: 137 130 2049
Fragments arranged by size:
2049 137 130
ApoI r'AATT_y
Cuts at: 0 1414 2316
Size: 1414 902
AvaI C'yCGr_G
Cuts at: 0 370 1138 1853 2316
Size: 370 768 715 463
Fragments arranged by size:
768 715 463 370
BamHI G'GATC_C
Cuts at: 0 1671 2316
Size: 1671 645
BanI G'GyrC_C
Cuts at: 0 282 723 1737 1976 2316
Size: 282 441 1014 239 340
Fragments arranged by size:
1014 441 340 282 239
BanII G_rGCy'C
Cuts at: 0 137 267 866 1247 1854 2316
Size: 137 130 599 381 607 462
Fragments arranged by size:
607 599 462 381 137 130
BbeI G_GCGC'C
Cuts at: 0 1980 2316
Size: 1980 336
BciVI GTATCCnnnnn_n'
Cuts at: 0 78 208 2316
Size: 78 130 2108
Fragments arranged by size:
2108 130 78
BglII A'GATC_T
Cuts at: 0 516 945 2316
Size: 516 429 1371
Fragments arranged by size:
1371 516 429
BlpI GC'TnA_GC
Cuts at: 0 1679 1954 2316
Size: 1679 275 362
Fragments arranged by size:
1679 362 275
Bme1580I G_kGCm'C
Cuts at: 0 137 267 1017 1749 1782 2316
Size: 137 130 750 732 33 534
Fragments arranged by size:
750 732 534 137 130 33
BmgBI CAC'GTC
Cuts at: 0 81 2316
Size: 81 2235
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 153 478 710 1736 1769 2198 2316
Size: 131 22 325 232 1026 33 429 118
Fragments arranged by size:
1026 429 325 232 131 118 33 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1043 1426 1431 1723 2065 2203 2316
Size: 22 1021 383 5 292 342 138 113
Fragments arranged by size:
1021 383 342 292 138 113 22 5
Bpu10I CC'TnA_GC
Cuts at: 0 1468 2316
Size: 1468 848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1768 2316
Size: 1768 548
BsaI GGTCTCn'nnnn_
Cuts at: 0 314 1019 1585 2316
Size: 314 705 566 731
Fragments arranged by size:
731 705 566 314
BsaAI yAC'GTr
Cuts at: 0 1710 2316
Size: 1710 606
BsaBI GATnn'nnATC
Cuts at: 0 526 548 2316
Size: 526 22 1768
Fragments arranged by size:
1768 526 22
BsaHI Gr'CG_yC
Cuts at: 0 1096 1977 2316
Size: 1096 881 339
Fragments arranged by size:
1096 881 339
BsaWI w'CCGG_w
Cuts at: 0 2306 2316
Size: 2306 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 884 1423 1494 1497 1921 2236 2316
Size: 238 646 539 71 3 424 315 80
Fragments arranged by size:
646 539 424 315 238 80 71 3
BseYI C'CCAG_C
Cuts at: 0 559 1192 1259 1400 1949 2316
Size: 559 633 67 141 549 367
Fragments arranged by size:
633 559 549 367 141 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1411 2316
Size: 701 710 905
Fragments arranged by size:
905 710 701
BsiHKAI G_wGCw'C
Cuts at: 0 1039 1709 1847 2316
Size: 1039 670 138 469
Fragments arranged by size:
1039 670 469 138
BsmI GAATG_Cn'
Cuts at: 0 810 2316
Size: 810 1506
Bsp1286I G_dGCh'C
Cuts at: 0 137 267 866 1017 1039 1247 1709 1749
Size: 137 130 599 151 22 208 462 40
Cuts at: 1749 1782 1847 1854 2316
Size: 33 65 7 462
Fragments arranged by size:
599 462 462 208 151 137 130 65
40 33 22 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 796 1772 1904 2072 2316
Size: 689 107 976 132 168 244
Fragments arranged by size:
976 689 244 168 132 107
BsrGI T'GTAC_A
Cuts at: 0 1007 2316
Size: 1007 1309
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1744 2316
Size: 1744 572
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1267 1749 2316
Size: 1267 482 567
Fragments arranged by size:
1267 567 482
BstYI r'GATC_y
Cuts at: 0 516 945 1599 1671 1865 2316
Size: 516 429 654 72 194 451
Fragments arranged by size:
654 516 451 429 194 72
Bsu36I CC'TnA_GG
Cuts at: 0 172 769 2316
Size: 172 597 1547
Fragments arranged by size:
1547 597 172
BtgI C'CryG_G
Cuts at: 0 974 2316
Size: 974 1342
BtsI GCAGTG_nn'
Cuts at: 0 233 851 943 2244 2316
Size: 233 618 92 1301 72
Fragments arranged by size:
1301 618 233 92 72
DraIII CAC_nnn'GTG
Cuts at: 0 1744 1777 2316
Size: 1744 33 539
Fragments arranged by size:
1744 539 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 750 2239 2316
Size: 750 1489 77
Fragments arranged by size:
1489 750 77
EaeI y'GGCC_r
Cuts at: 0 1160 1364 2264 2316
Size: 1160 204 900 52
Fragments arranged by size:
1160 900 204 52
EarI CTCTTCn'nnn_
Cuts at: 0 755 878 902 1135 1252 1500 1515 1521
Size: 755 123 24 233 117 248 15 6
Cuts at: 1521 1548 1601 2316
Size: 27 53 715
Fragments arranged by size:
755 715 248 233 123 117 53 27
24 15 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 31 1043 1116 1426 1431 1499 1723
Size: 22 9 1012 73 310 5 68 224
Cuts at: 1723 2065 2203 2316
Size: 342 138 113
Fragments arranged by size:
1012 342 310 224 138 113 73 68
22 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 1090 2152 2316
Size: 253 837 1062 164
Fragments arranged by size:
1062 837 253 164
EcoO109I rG'GnC_Cy
Cuts at: 0 133 263 264 2061 2316
Size: 133 130 1 1797 255
Fragments arranged by size:
1797 255 133 130 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 36 784 2316
Size: 36 748 1532
Fragments arranged by size:
1532 748 36
HaeII r_GCGC'y
Cuts at: 0 1980 2316
Size: 1980 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 937 969 1411 1443 1584 1616 2316
Size: 937 32 442 32 141 32 700
Fragments arranged by size:
937 700 442 141 32 32 32
KasI G'GCGC_C
Cuts at: 0 1976 2316
Size: 1976 340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 2316
Size: 1319 997
MscI TGG'CCA
Cuts at: 0 1162 1366 2316
Size: 1162 204 950
Fragments arranged by size:
1162 950 204
MslI CAynn'nnrTG
Cuts at: 0 964 979 2316
Size: 964 15 1337
Fragments arranged by size:
1337 964 15
MspA1I CmG'CkG
Cuts at: 0 1404 1953 2316
Size: 1404 549 363
Fragments arranged by size:
1404 549 363
NarI GG'CG_CC
Cuts at: 0 1977 2316
Size: 1977 339
NcoI C'CATG_G
Cuts at: 0 974 2316
Size: 974 1342
PasI CC'CwG_GG
Cuts at: 0 1101 2316
Size: 1101 1215
PflMI CCAn_nnn'nTGG
Cuts at: 0 490 2316
Size: 490 1826
PfoI T'CCnGG_A
Cuts at: 0 116 1020 1817 2316
Size: 116 904 797 499
Fragments arranged by size:
904 797 499 116
PmlI CAC'GTG
Cuts at: 0 1710 2316
Size: 1710 606
PpuMI rG'GwC_Cy
Cuts at: 0 2061 2316
Size: 2061 255
PshAI GACnn'nnGTC
Cuts at: 0 581 2316
Size: 581 1735
PspOMI G'GGCC_C
Cuts at: 0 133 263 2316
Size: 133 130 2053
Fragments arranged by size:
2053 133 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 2316
Size: 1633 32 651
Fragments arranged by size:
1633 651 32
PstI C_TGCA'G
Cuts at: 0 1114 2069 2316
Size: 1114 955 247
Fragments arranged by size:
1114 955 247
PvuII CAG'CTG
Cuts at: 0 1404 1953 2316
Size: 1404 549 363
Fragments arranged by size:
1404 549 363
SapI GCTCTTCn'nnn_
Cuts at: 0 878 1252 2316
Size: 878 374 1064
Fragments arranged by size:
1064 878 374
SfcI C'TryA_G
Cuts at: 0 473 579 1044 1110 1575 2065 2316
Size: 473 106 465 66 465 490 251
Fragments arranged by size:
490 473 465 465 251 106 66
SfoI GGC'GCC
Cuts at: 0 1978 2316
Size: 1978 338
SmlI C'TyrA_G
Cuts at: 0 370 1783 2316
Size: 370 1413 533
Fragments arranged by size:
1413 533 370
StuI AGG'CCT
Cuts at: 0 362 1396 1433 2316
Size: 362 1034 37 883
Fragments arranged by size:
1034 883 362 37
StyI C'CwwG_G
Cuts at: 0 182 974 2316
Size: 182 792 1342
Fragments arranged by size:
1342 792 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1755 1788 2316
Size: 1755 33 528
Fragments arranged by size:
1755 528 33
TatI w'GTAC_w
Cuts at: 0 1007 2316
Size: 1007 1309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 922 954 2316
Size: 922 32 1362
Fragments arranged by size:
1362 922 32
Tth111I GACn'n_nGTC
Cuts at: 0 912 2316
Size: 912 1404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2040 2161 2316
Size: 2040 121 155
Fragments arranged by size:
2040 155 121
XhoI C'TCGA_G
Cuts at: 0 370 2316
Size: 370 1946
XmnI GAAnn'nnTTC
Cuts at: 0 32 2316
Size: 32 2284
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BciVI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NcoI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI
PvuII SapI SfcI SfoI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BcgI BclI BfrBI BglI
BmtI BplI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PsiI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XmaI ZraI