Restriction map for
pFN21ASDF0201
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AlwI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MamI MfeI MflI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 71 CGCG CG^CG
AcsI 1777 AAATTT [GA]^AATT[CT]
AflIII 1895 ACGTGT A^C[GA][CT]GT
Alw44I 1876 GTGCAC G^TGCAC
ApaLI 1876 GTGCAC G^TGCAC
ApoI 1777 AAATTT [GA]^AATT[CT]
AspI 2011 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1800 TGGCCA TGG^CCA
BbsI 24 GTCTTC GAAGAC(2/6)
BcgI 1701 CGAGCTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1193 CTAG C^TAG
BpuAI 24 GTCTTC GAAGAC(2/6)
BsaWI 1784 ACCGGA [AT]^CCGG[AT]
BseRI 2076 CTCCTC GAGGAG(10/8)
BsgI 2023 GTGCAG GTGCAG(16/14)
BsmI 1172 GCATTC GAATGC(1/-1)
Bsp1407I 1910 TGTACA T^GTACA
BsrGI 1910 TGTACA T^GTACA
BstEII 1180 GGTGACC G^GT[ACGT]ACC
BstPI 1180 GGTGACC G^GT[ACGT]ACC
BstUI 71 CGCG CG^CG
BstXI 1971 CCAGGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 601 GATC ^GATC
EcoNI 1965 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1180 GGTGACC G^GT[ACGT]ACC
KspI 70 CCGCGG CCGC^GG
MaeI 1193 CTAG C^TAG
MboI 601 GATC ^GATC
MluNI 1800 TGGCCA TGG^CCA
MscI 1800 TGGCCA TGG^CCA
MvnI 71 CGCG CG^CG
NaeI 1926 GCCGGC GCC^GGC
NdeII 601 GATC ^GATC
NgoMIV 1926 GCCGGC G^CCGGC
NspI 1766 GCATGC [GA]CATG^[CT]
PflFI 2011 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1094 CTGCAG CTGCA^G
SacII 70 CCGCGG CCGC^GG
Sau3AI 601 GATC ^GATC
SfcI 1094 CTGCAG C^T[GA][CT]AG
SmlI 610 CTCAAG C^T[CT][GA]AG
SphI 1766 GCATGC GCATG^C
SspBI 1910 TGTACA T^GTACA
Tsp509I 1778 AATT ^AATT
Tth111I 2011 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AccI 829 GTAGAC GT^[AC][GT]AC
1855 GTCTAC
AcyI 75 GGCGTC G[GA]^CG[CT]C
1852 GGCGTC
BsaHI 75 GGCGTC G[GA]^CG[CT]C
1852 GGCGTC
BsmAI 746 GAGAC GTCTC(1/5)
1612 GAGAC
BspMI 1634 ACCTGC ACCTGC(4/8)
1837 ACCTGC
DrdI 15 GACACCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1397 GACTCCAGGGTC
EaeI 1800 TGGCCA [CT]^GGCC[GA]
1928 CGGCCA
HgaI 76 GCGTC GACGC(5/10)
1853 GCGTC
Hin1I 75 GGCGTC G[GA]^CG[CT]C
1852 GGCGTC
MaeIII 1181 GTGAC ^GT[ACGT]AC
1343 GTGAC
MboII 25 TCTTC GAAGA(8/7)
1832 TCTTC
NcoI 321 CCATGG C^CATGG
1248 CCATGG
Tsp45I 1181 GTGAC ^GT[GC]AC
1343 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwNI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr10I Cfr13I DdeI DraII DsaI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MnlI
MslI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau96I ScrFI
SexAI SfaNI SfiI SmaI StuI
StyI TaqI TfiI TseI TspRI
TthHB8I Van91I XcmI XmaCI XmaI