(Circular) (Six-base) MAPSORT of: pFN21ASDF0201.seq Check: 5549 from: 1 to: 6838 pFN21ASDF0201 6838 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3666 3869 3666 Size: 203 6635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4648 278 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2853 3879 4181 1937 Size: 916 1026 302 4594 Fragments arranged by size: 4594 1026 916 302 Acc65I G'GTAC_C Cuts at: 4159 4159 Size: 6838 AclI AA'CG_TT Cuts at: 1563 4965 5338 1563 Size: 3402 373 3063 Fragments arranged by size: 3402 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 3488 4901 6285 6553 1632 Size: 635 1221 1413 1384 268 1917 Fragments arranged by size: 1917 1413 1384 1221 635 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6641 AflIII A'CryG_T Cuts at: 1203 3918 5737 6714 1203 Size: 2715 1819 977 1327 Fragments arranged by size: 2715 1819 1327 977 AgeI A'CCGG_T Cuts at: 5669 5669 Size: 6838 AhdI GACnn_n'nnGTC Cuts at: 5567 5567 Size: 6838 AleI CACnn'nnGTG Cuts at: 1166 3758 3917 1166 Size: 2592 159 4087 Fragments arranged by size: 4087 2592 159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2706 1184 Size: 1522 5316 AlwNI CAG_nnn'CTG Cuts at: 2212 2825 3170 3848 4581 6153 2212 Size: 613 345 678 733 1572 2897 Fragments arranged by size: 2897 1572 733 678 613 345 ApaI G_GGCC'C Cuts at: 2370 2519 2571 2598 2616 2677 2764 2834 2903 Size: 149 52 27 18 61 87 70 69 Cuts at: 2903 2939 3008 3080 3144 3271 3297 3450 3495 Size: 36 69 72 64 127 26 153 45 Cuts at: 3495 3537 3546 3581 3600 2370 Size: 42 9 35 19 5608 Fragments arranged by size: 5608 153 149 127 87 72 70 69 69 64 61 52 45 42 36 35 27 26 19 18 9 ApaLI G'TGCA_C Cuts at: 3899 4895 6051 3899 Size: 996 1156 4686 Fragments arranged by size: 4686 1156 996 ApoI r'AATT_y Cuts at: 1493 3800 4147 4373 4525 6634 1493 Size: 2307 347 226 152 2109 1697 Fragments arranged by size: 2307 2109 1697 347 226 152 AseI AT'TA_AT Cuts at: 160 1032 5392 160 Size: 872 4360 1606 Fragments arranged by size: 4360 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 AvaI C'yCGr_G Cuts at: 1870 1943 2276 2283 2512 2660 2931 3593 3601 Size: 73 333 7 229 148 271 662 8 Cuts at: 3601 3784 4163 1870 Size: 183 379 4545 Fragments arranged by size: 4545 662 379 333 271 229 183 148 73 8 7 BamHI G'GATC_C Cuts at: 4168 4535 4168 Size: 367 6471 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2549 2560 2690 3614 3680 Size: 325 217 627 762 11 130 924 66 Cuts at: 3680 3911 4159 5614 618 Size: 231 248 1455 1842 Fragments arranged by size: 1842 1455 924 762 627 325 248 231 217 130 66 11 BanII G_rGCy'C Cuts at: 1391 2259 2370 2412 2519 2571 2598 2616 2677 Size: 868 111 42 107 52 27 18 61 Cuts at: 2677 2764 2834 2903 2939 3008 3080 3144 3271 Size: 87 70 69 36 69 72 64 127 Cuts at: 3271 3297 3352 3378 3450 3495 3537 3546 3581 Size: 26 55 26 72 45 42 9 35 Cuts at: 3581 3600 4157 1391 Size: 19 557 4072 Fragments arranged by size: 4072 868 557 127 111 107 87 72 72 70 69 69 64 61 55 52 45 42 42 36 35 27 26 26 19 18 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2040 928 Size: 971 141 5726 Fragments arranged by size: 5726 971 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3713 3747 5029 5063 1903 Size: 34 1776 34 1282 34 3678 Fragments arranged by size: 3678 1776 1282 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4730 5940 4730 Size: 1210 5628 BclI T'GATC_A Cuts at: 1548 1548 Size: 6838 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 2304 2434 Size: 107 122 71 631 605 451 181 130 Cuts at: 2434 2514 2523 2706 2961 3148 3462 3541 3595 Size: 80 9 183 255 187 314 79 54 Cuts at: 3595 3623 3746 3831 3870 5449 136 Size: 28 123 85 39 1579 1525 Fragments arranged by size: 1579 1525 631 605 451 314 255 187 183 181 130 123 122 107 85 80 79 71 54 39 28 9 BglII A'GATC_T Cuts at: 6833 6833 Size: 6838 BlpI GC'TnA_GC Cuts at: 4255 4255 Size: 6838 Bme1580I G_kGCm'C Cuts at: 1790 2370 2519 2554 2571 2598 2616 2677 2695 Size: 580 149 35 17 27 18 61 18 Cuts at: 2695 2764 2834 2903 2939 3008 3080 3144 3271 Size: 69 70 69 36 69 72 64 127 Cuts at: 3271 3297 3450 3495 3537 3546 3581 3600 3619 Size: 26 153 45 42 9 35 19 19 Cuts at: 3619 3903 3914 4899 6055 1790 Size: 284 11 985 1156 2573 Fragments arranged by size: 2573 1156 985 580 284 153 149 127 72 70 69 69 69 64 61 45 42 36 35 35 27 26 19 19 18 18 17 11 9 BmgBI CAC'GTC Cuts at: 1318 3137 3904 1318 Size: 1819 767 4252 Fragments arranged by size: 4252 1819 767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 3300 4569 Size: 416 53 83 399 24 718 1165 1269 Cuts at: 4569 5527 442 Size: 958 1753 Fragments arranged by size: 1753 1269 1165 958 718 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6838 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2814 2868 3192 3348 3407 3519 4001 5498 Size: 1575 54 324 156 59 112 482 1497 Cuts at: 5498 1239 Size: 2579 Fragments arranged by size: 2579 1575 1497 482 324 156 112 59 54 Bpu10I CC'TnA_GC Cuts at: 2728 2728 Size: 6838 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2618 4963 5828 6126 2618 Size: 2345 865 298 3330 Fragments arranged by size: 3330 2345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5501 882 Size: 617 4002 2219 Fragments arranged by size: 4002 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4135 4629 493 Size: 711 2931 494 2702 Fragments arranged by size: 2931 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6832 6832 Size: 6838 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2099 3876 4645 5027 Size: 53 83 186 936 566 1777 769 382 Cuts at: 5027 5662 275 Size: 635 1451 Fragments arranged by size: 1777 1451 936 769 635 566 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3807 5271 5669 5943 6090 6501 3807 Size: 1464 398 274 147 411 4144 Fragments arranged by size: 4144 1464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3565 3595 4082 4112 1178 Size: 30 165 30 2162 30 487 30 3904 Fragments arranged by size: 3904 2162 487 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4090 1186 Size: 2904 3934 BseYI C'CCAG_C Cuts at: 2026 2080 2363 3007 3011 3227 3530 3688 3718 Size: 54 283 644 4 216 303 158 30 Cuts at: 3718 3841 6041 6537 2026 Size: 123 2200 496 2327 Fragments arranged by size: 2327 2200 644 496 303 283 216 158 123 54 30 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4067 1163 Size: 573 2331 3934 Fragments arranged by size: 3934 2331 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4288 5049 6077 1540 Size: 275 186 2287 761 1028 2301 Fragments arranged by size: 2301 2287 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3765 3903 4017 4899 4984 6055 3765 Size: 138 114 882 85 1071 4548 Fragments arranged by size: 4548 1071 882 138 114 85 BsmI GAATG_Cn' Cuts at: 3195 4349 4442 3195 Size: 1154 93 5591 Fragments arranged by size: 5591 1154 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2370 2412 2519 2554 2571 2598 Size: 399 469 111 42 107 35 17 27 Cuts at: 2598 2616 2677 2695 2764 2834 2903 2939 3008 Size: 18 61 18 69 70 69 36 69 Cuts at: 3008 3080 3144 3271 3297 3352 3378 3450 3495 Size: 72 64 127 26 55 26 72 45 Cuts at: 3495 3537 3546 3581 3600 3619 3765 3903 3914 Size: 42 9 35 19 19 146 138 11 Cuts at: 3914 4017 4157 4899 4984 6055 1391 Size: 103 140 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 469 399 146 140 138 127 111 107 103 85 72 72 70 69 69 69 64 61 55 45 42 42 36 35 35 27 26 26 19 19 18 18 17 11 9 BspEI T'CCGG_A Cuts at: 6501 6501 Size: 6838 BspHI T'CATG_A Cuts at: 4727 6457 4727 Size: 1730 5108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3666 3869 4193 844 Size: 2822 203 324 3489 Fragments arranged by size: 3489 2822 324 203 BsrBI CCG'CTC Cuts at: 1394 1823 4725 1394 Size: 429 2902 3507 Fragments arranged by size: 3507 2902 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5333 5507 65 Size: 1183 4085 174 1396 Fragments arranged by size: 4085 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2117 3745 3949 5482 5669 1700 Size: 110 307 1628 204 1533 187 2869 Fragments arranged by size: 2869 1628 1533 307 204 187 110 BsrGI T'GTAC_A Cuts at: 96 3933 96 Size: 3837 3001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6838 BssSI C'ACGA_G Cuts at: 4898 5910 4898 Size: 1012 5826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6838 BstBI TT'CG_AA Cuts at: 2007 5642 2007 Size: 3635 3203 BstEII G'GTnAC_C Cuts at: 1184 3203 1184 Size: 2019 4819 BstXI CCAn_nnnn'nTGG Cuts at: 4001 4001 Size: 6838 BstYI r'GATC_y Cuts at: 1972 4168 4535 4920 4937 6389 6475 6487 6833 Size: 2196 367 385 17 1452 86 12 346 Cuts at: 6833 1972 Size: 1977 Fragments arranged by size: 2196 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2521 3153 3395 3406 2521 Size: 632 242 11 5953 Fragments arranged by size: 5953 632 242 11 BtgI C'CryG_G Cuts at: 513 1065 2093 2344 3271 3537 4083 513 Size: 552 1028 251 927 266 546 3268 Fragments arranged by size: 3268 1028 927 552 546 266 251 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3628 487 Size: 683 56 2402 3697 Fragments arranged by size: 3697 2402 683 56 BtsI GCAGTG_nn' Cuts at: 3753 4354 5148 5168 3753 Size: 601 794 20 5423 Fragments arranged by size: 5423 794 601 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6838 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6803 DraI TTT'AAA Cuts at: 4142 4142 Size: 6838 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2044 3426 5845 809 Size: 507 728 1382 2419 1802 Fragments arranged by size: 2419 1802 1382 728 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3823 3951 4285 Size: 54 1425 50 275 18 1993 128 334 Cuts at: 4285 5174 6803 8 Size: 889 1629 43 Fragments arranged by size: 1993 1629 1425 889 334 275 128 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4285 1537 Size: 275 2473 4090 Fragments arranged by size: 4090 2473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4768 1353 Size: 3415 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5425 5799 5945 387 Size: 1064 138 3836 374 146 1280 Fragments arranged by size: 3836 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2814 2868 3192 3348 3407 3488 Size: 393 635 547 54 324 156 59 81 Cuts at: 3488 3519 4001 4901 5498 6285 6553 1239 Size: 31 482 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 635 597 547 482 393 324 268 156 81 59 54 31 EcoNI CCTnn'n_nnAGG Cuts at: 3992 3992 Size: 6838 EcoO109I rG'GnC_Cy Cuts at: 2171 2288 2367 2479 2489 2498 2516 2567 2568 Size: 117 79 112 10 9 18 51 1 Cuts at: 2568 2594 2595 2613 2673 2674 2760 2761 2804 Size: 26 1 18 60 1 86 1 43 Cuts at: 2804 2840 2899 2928 2935 2936 2954 2987 3004 Size: 36 59 29 7 1 18 33 17 Cuts at: 3004 3016 3076 3095 3141 3167 3176 3212 3241 Size: 12 60 19 46 26 9 36 29 Cuts at: 3241 3267 3401 3447 3491 3492 3542 3543 3553 Size: 26 134 46 44 1 50 1 10 Cuts at: 3553 3578 3587 3597 3605 3624 2171 Size: 25 9 10 8 19 5385 Fragments arranged by size: 5385 134 117 112 86 79 60 60 59 51 50 46 46 44 43 36 36 33 29 29 26 26 26 25 19 19 18 18 18 17 12 10 10 10 9 9 9 8 7 1 1 1 1 1 1 1 EcoRI G'AATT_C Cuts at: 4147 4525 4147 Size: 378 6460 FspI TGC'GCA Cuts at: 5344 5344 Size: 6838 HaeII r_GCGC'y Cuts at: 1678 5985 1678 Size: 4307 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3983 4015 4104 4136 5479 Size: 32 135 32 2411 32 89 32 1343 Cuts at: 5479 5511 5553 5585 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2411 1343 135 89 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4182 4428 669 Size: 988 281 2244 246 3079 Fragments arranged by size: 3079 2244 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6838 HpaI GTT'AAC Cuts at: 4428 4428 Size: 6838 KpnI G_GTAC'C Cuts at: 4163 4163 Size: 6838 MfeI C'AATT_G Cuts at: 4437 4437 Size: 6838 MluI A'CGCG_T Cuts at: 6714 6714 Size: 6838 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4038 5952 6136 1305 Size: 2733 1914 184 2007 Fragments arranged by size: 2733 2007 1914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3825 10 Size: 54 1425 343 1993 3023 Fragments arranged by size: 3023 1993 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3758 3794 3917 4796 5155 5314 518 Size: 648 2592 36 123 879 359 159 2042 Fragments arranged by size: 2592 2042 879 648 359 159 123 36 MspA1I CmG'CkG Cuts at: 1606 2095 2230 2723 3011 3434 3831 4021 4254 Size: 489 135 493 288 423 397 190 233 Cuts at: 4254 4931 6079 6324 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 493 489 423 397 288 245 233 190 135 NaeI GCC'GGC Cuts at: 3951 3951 Size: 6838 NcoI C'CATG_G Cuts at: 513 1065 2344 3271 513 Size: 552 1279 927 4080 Fragments arranged by size: 4080 1279 927 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6838 NgoMIV G'CCGG_C Cuts at: 3949 3949 Size: 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6838 NotI GC'GGCC_GC Cuts at: 4285 4285 Size: 6838 NspI r_CATG'y Cuts at: 1132 1229 3793 4196 5741 1132 Size: 97 2564 403 1545 2229 Fragments arranged by size: 2564 2229 1545 403 97 PasI CC'CwG_GG Cuts at: 1348 2267 2405 2599 2678 2808 2870 2942 3146 Size: 919 138 194 79 130 62 72 204 Cuts at: 3146 3352 3380 3548 3620 1348 Size: 206 28 168 72 4566 Fragments arranged by size: 4566 919 206 204 194 168 138 130 79 72 72 62 28 PciI A'CATG_T Cuts at: 5737 5737 Size: 6838 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 3713 3848 3921 1108 Size: 1104 1501 135 73 4025 Fragments arranged by size: 4025 1501 1104 135 73 PfoI T'CCnGG_A Cuts at: 2381 2791 2845 2381 Size: 410 54 6374 Fragments arranged by size: 6374 410 54 PmeI GTTT'AAAC Cuts at: 4142 4142 Size: 6838 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4907 4939 6420 6452 4907 Size: 32 1481 32 5293 Fragments arranged by size: 5293 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2171 2288 2489 2498 2840 2928 3016 3095 3176 Size: 117 201 9 342 88 88 79 81 Cuts at: 3176 3241 3401 3553 3605 2171 Size: 65 160 152 52 5404 Fragments arranged by size: 5404 342 201 160 152 117 88 88 81 79 65 52 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6838 PsiI TTA'TAA Cuts at: 4408 4408 Size: 6838 PspOMI G'GGCC_C Cuts at: 2366 2515 2567 2594 2612 2673 2760 2830 2899 Size: 149 52 27 18 61 87 70 69 Cuts at: 2899 2935 3004 3076 3140 3267 3293 3446 3491 Size: 36 69 72 64 127 26 153 45 Cuts at: 3491 3533 3542 3577 3596 2366 Size: 42 9 35 19 5608 Fragments arranged by size: 5608 153 149 127 87 72 70 69 69 64 61 52 45 42 36 35 27 26 19 18 9 PstI C_TGCA'G Cuts at: 830 3121 4190 830 Size: 2291 1069 3478 Fragments arranged by size: 3478 2291 1069 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6838 PvuII CAG'CTG Cuts at: 2723 3011 3434 4021 2723 Size: 288 423 587 5540 Fragments arranged by size: 5540 587 423 288 SacII CC_GC'GG Cuts at: 2096 2096 Size: 6838 SalI G'TCGA_C Cuts at: 1936 4180 1936 Size: 2244 4594 SanDI GG'GwC_CC Cuts at: 2288 2489 2928 3016 3095 3241 3401 3553 3605 Size: 201 439 88 79 146 160 152 52 Cuts at: 3605 2288 Size: 5521 Fragments arranged by size: 5521 439 201 160 152 146 88 79 52 SbfI CC_TGCA'GG Cuts at: 4190 4190 Size: 6838 ScaI AGT'ACT Cuts at: 5086 6798 5086 Size: 1712 5126 SexAI A'CCwGG_T Cuts at: 2545 2737 4035 2545 Size: 192 1298 5348 Fragments arranged by size: 5348 1298 192 SfcI C'TryA_G Cuts at: 826 1045 3117 4186 5321 6002 6193 826 Size: 219 2072 1069 1135 681 191 1471 Fragments arranged by size: 2072 1471 1135 1069 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2514 2706 3148 3462 3541 3595 2514 Size: 192 442 314 79 54 5757 Fragments arranged by size: 5757 442 314 192 79 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6838 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6838 SmaI CCC'GGG Cuts at: 1872 2278 2514 2662 2933 3595 3603 4165 1872 Size: 406 236 148 271 662 8 562 4545 Fragments arranged by size: 4545 662 562 406 271 236 148 8 SmlI C'TyrA_G Cuts at: 820 1017 1943 2633 4942 5843 6105 820 Size: 197 926 690 2309 901 262 1553 Fragments arranged by size: 2309 1553 926 901 690 262 197 SnaBI TAC'GTA Cuts at: 493 4135 4629 493 Size: 3642 494 2702 Fragments arranged by size: 3642 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6838 SphI G_CATG'C Cuts at: 1132 3793 4196 1132 Size: 2661 403 3774 Fragments arranged by size: 3774 2661 403 SrfI GCCC'GGGC Cuts at: 2278 2662 2278 Size: 384 6454 SspI AAT'ATT Cuts at: 5 52 4762 5 Size: 47 4710 2081 Fragments arranged by size: 4710 2081 47 StuI AGG'CCT Cuts at: 1517 2631 3456 3483 1517 Size: 1114 825 27 4872 Fragments arranged by size: 4872 1114 825 27 StyI C'CwwG_G Cuts at: 513 1065 2344 2554 2991 3271 3297 3451 4277 Size: 552 1279 210 437 280 26 154 826 Cuts at: 4277 513 Size: 3074 Fragments arranged by size: 3074 1279 826 552 437 280 210 154 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3913 4033 4865 4882 5035 5220 5665 Size: 1255 2333 120 832 17 153 185 445 Cuts at: 5665 325 Size: 1498 Fragments arranged by size: 2333 1498 1255 832 445 185 153 120 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 3933 3972 Size: 274 80 33 51 1443 171 1785 39 Cuts at: 3972 4089 5084 6796 96 Size: 117 995 1712 138 Fragments arranged by size: 1785 1712 1443 995 274 171 138 117 80 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3324 3356 3565 3597 6798 6830 3324 Size: 32 209 32 3201 32 3332 Fragments arranged by size: 3332 3201 209 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4037 1316 Size: 2721 4117 XbaI T'CTAG_A Cuts at: 4174 4174 Size: 6838 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2351 2606 3215 3278 3431 3670 3922 4001 2351 Size: 255 609 63 153 239 252 79 5188 Fragments arranged by size: 5188 609 255 252 239 153 79 63 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6838 XmaI C'CCGG_G Cuts at: 1870 2276 2512 2660 2931 3593 3601 4163 1870 Size: 406 236 148 271 662 8 562 4545 Fragments arranged by size: 4545 662 562 406 271 236 148 8 XmnI GAAnn'nnTTC Cuts at: 4967 4967 Size: 6838 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4646 276 Size: 53 83 186 936 3112 2468 Fragments arranged by size: 3112 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfoI SwaI