Restriction map for
pFN21ASDF0002
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII AccIII AclI AflII Alw44I
ApaLI AscI AseI AsnI AspI
AvrII BbvCI BcgI BfrI BglII
BlnI BsaBI BseAI BsiEI BsiWI
BsmBI BspEI BspHI BspLU11I BssHII
BstBI BstEII BstPI CpoI DraI
DraIII DrdI EagI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1299 GGTACC G^GTACC
AcsI 2931 AAATTC [GA]^AATT[CT]
AflIII 2612 ACGTGT A^C[GA][CT]GT
AgeI 1539 ACCGGT A^CCGGT
AhdI 1484 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3490 AGCGCT AGC^GCT
ApoI 2931 AAATTC [GA]^AATT[CT]
Asp700I 4 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1299 GGTACC G^GTACC
AspEI 1484 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4397 TGCGCA TGC^GCA
BciVI 2126 GTATCC GTATCC(6/5)
BclI 1670 TGATCA T^GATCA
Bsp1407I 2081 TGTACA T^GTACA
BspDI 4213 ATCGAT AT^CGAT
BsrGI 2081 TGTACA T^GTACA
Bst1107I 1519 GTATAC GTA^TAC
BstAPI 4425 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3932 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1519 GTATAC GTA^TAC
ClaI 4213 ATCGAT AT^CGAT
Eam1105I 1484 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3490 AGCGCT AGC^GCT
FbaI 1670 TGATCA T^GATCA
FspI 4397 TGCGCA TGC^GCA
HincII 1306 GTCAAC GT[CT]^[GA]AC
HindII 1306 GTCAAC GT[CT]^[GA]AC
KpnI 1299 GGTACC GGTAC^C
NheI 987 GCTAGC G^CTAGC
PaeR7I 1469 CTCGAG C^TCGAG
PinAI 1539 ACCGGT A^CCGGT
PshAI 2477 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 389 CCCGGG CCC^GGG
SphI 2312 GCATGC GCATG^C
SspBI 2081 TGTACA T^GTACA
Tsp509I 2932 AATT ^AATT
XhoI 1469 CTCGAG C^TCGAG
XmaCI 389 CCCGGG C^CCGGG
XmaI 389 CCCGGG C^CCGGG
XmnI 4 GAACGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1519 GTATAC GT^[AC][GT]AC
2218 GTCTAC
AocI 631 CCTGAGG CC^T[ACGT]AGG
730 CCTCAGG
BbrPI 2611 CACGTG CAC^GTG
3238 CACGTG
BlpI 99 GCTCAGC GC^T[ACGT]AGC
2316 GCTGAGC
Bpu1102I 99 GCTCAGC GC^T[ACGT]AGC
2316 GCTGAGC
BsaAI 2611 CACGTG [CT]AC^GT[GA]
3238 CACGTG
BsaWI 1539 ACCGGT [AT]^CCGG[AT]
1706 ACCGGA
BsrBI 46 CCGCTC CCGCTC(-3/-3)
3646 GAGCGG
Bsu36I 631 CCTGAGG CC^T[ACGT]AGG
730 CCTCAGG
CelII 99 GCTCAGC GC^T[ACGT]AGC
2316 GCTGAGC
Eco81I 631 CCTGAGG CC^T[ACGT]AGG
730 CCTCAGG
HgaI 2374 GACGC GACGC(5/10)
3641 GCGTC
HindIII 1378 AAGCTT A^AGCTT
2621 AAGCTT
MseI 1175 TTAA T^TAA
1376 TTAA
NcoI 503 CCATGG C^CATGG
4100 CCATGG
NspI 1965 GCATGT [GA]CATG^[CT]
2312 GCATGC
PmaCI 2611 CACGTG CAC^GTG
3238 CACGTG
PmlI 2611 CACGTG CAC^GTG
3238 CACGTG
SfiI 699 GGCCCGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3354 GGCCACAGGGGCC
SmlI 1469 CTCGAG C^T[CT][GA]AG
3400 CTTGAG
Tru9I 1175 TTAA T^TAA
1376 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII