(Circular) (Six-base) MAPSORT of: pFN21ASDF0002.seq Check: 2666 from: 1 to: 9205 pFN21ASDF0002 9205 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4320 4320 Size: 9205 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7015 278 Size: 53 83 186 936 5479 2468 Fragments arranged by size: 5479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3543 4242 6548 1937 Size: 1606 699 2306 4594 Fragments arranged by size: 4594 2306 1606 699 Acc65I G'GTAC_C Cuts at: 3322 6526 3322 Size: 3204 6001 AclI AA'CG_TT Cuts at: 1563 7332 7705 1563 Size: 5769 373 3063 Fragments arranged by size: 5769 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2685 2915 3065 4781 6110 7268 8652 8920 Size: 1053 230 150 1716 1329 1158 1384 268 Cuts at: 8920 1632 Size: 1917 Fragments arranged by size: 1917 1716 1384 1329 1158 1053 268 230 150 AfeI AGC'GCT Cuts at: 5515 5515 Size: 9205 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9008 AflIII A'CryG_T Cuts at: 1203 4635 8104 9081 1203 Size: 3432 3469 977 1327 Fragments arranged by size: 3469 3432 1327 977 AgeI A'CCGG_T Cuts at: 3562 8036 3562 Size: 4474 4731 AhdI GACnn_n'nnGTC Cuts at: 3512 7934 3512 Size: 4422 4783 AleI CACnn'nnGTG Cuts at: 1166 2839 3629 6105 1166 Size: 1673 790 2476 4266 Fragments arranged by size: 4266 2476 1673 790 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2519 2577 3405 1184 Size: 1335 58 828 6984 Fragments arranged by size: 6984 1335 828 58 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4029 4061 4029 Size: 32 9173 AlwNI CAG_nnn'CTG Cuts at: 2163 2597 3463 5473 5722 6088 6451 6454 6948 Size: 434 866 2010 249 366 363 3 494 Cuts at: 6948 8520 2163 Size: 1572 2848 Fragments arranged by size: 2848 2010 1572 866 494 434 366 363 249 3 ApaI G_GGCC'C Cuts at: 2526 2931 3138 2526 Size: 405 207 8593 Fragments arranged by size: 8593 405 207 ApaLI G'TGCA_C Cuts at: 7262 8418 7262 Size: 1156 8049 ApoI r'AATT_y Cuts at: 1493 4954 6514 6740 6892 9001 1493 Size: 3461 1560 226 152 2109 1697 Fragments arranged by size: 3461 2109 1697 1560 226 152 AseI AT'TA_AT Cuts at: 160 1032 7759 160 Size: 872 6727 1606 Fragments arranged by size: 6727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9205 AvaI C'yCGr_G Cuts at: 1870 1943 2179 2412 2639 3471 3492 5617 6530 Size: 73 236 233 227 832 21 2125 913 Cuts at: 6530 1870 Size: 4545 Fragments arranged by size: 4545 2125 913 832 236 233 227 73 21 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4132 4165 4132 Size: 33 9172 BamHI G'GATC_C Cuts at: 3732 4044 5091 6535 6902 3732 Size: 312 1047 1444 367 6035 Fragments arranged by size: 6035 1444 1047 367 312 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2174 2220 2710 3322 3609 Size: 325 217 627 387 46 490 612 287 Cuts at: 3609 4422 4619 4997 5053 5301 5439 5732 6526 Size: 813 197 378 56 248 138 293 794 Cuts at: 6526 7981 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 813 794 627 612 490 387 378 325 293 287 248 217 197 138 56 46 BanII G_rGCy'C Cuts at: 1391 2526 2623 2631 2745 2931 3138 3472 3493 Size: 1135 97 8 114 186 207 334 21 Cuts at: 3493 3944 3972 4260 4821 5190 5207 5919 6012 Size: 451 28 288 561 369 17 712 93 Cuts at: 6012 6208 6524 1391 Size: 196 316 4072 Fragments arranged by size: 4072 1135 712 561 451 369 334 316 288 207 196 186 114 97 93 28 21 17 8 BbeI G_GCGC'C Cuts at: 2224 4426 4623 5305 2224 Size: 2202 197 682 6124 Fragments arranged by size: 6124 2202 682 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2248 2800 4091 5874 6272 928 Size: 971 349 552 1291 1783 398 3861 Fragments arranged by size: 3861 1783 1291 971 552 398 349 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7396 7430 1903 Size: 34 5459 34 3678 Fragments arranged by size: 5459 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 7097 8307 4160 Size: 2937 1210 5058 Fragments arranged by size: 5058 2937 1210 BclI T'GATC_A Cuts at: 1548 3693 1548 Size: 2145 7060 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2493 2708 2729 Size: 107 122 71 631 605 821 215 21 Cuts at: 2729 3020 3488 5171 5384 5868 7816 136 Size: 291 468 1683 213 484 1948 1525 Fragments arranged by size: 1948 1683 1525 821 631 605 484 468 291 215 213 122 107 71 21 BglII A'GATC_T Cuts at: 9200 9200 Size: 9205 BlpI GC'TnA_GC Cuts at: 2123 4340 6622 2123 Size: 2217 2282 4706 Fragments arranged by size: 4706 2282 2217 Bme1580I G_kGCm'C Cuts at: 1790 2177 2526 2836 2931 3138 3612 4219 5735 Size: 387 349 310 95 207 474 607 1516 Cuts at: 5735 7266 8422 1790 Size: 1531 1156 2573 Fragments arranged by size: 2573 1531 1516 1156 607 474 387 349 310 207 95 BmgBI CAC'GTC Cuts at: 1318 5773 1318 Size: 4455 4750 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2591 3162 3473 Size: 416 53 83 399 24 1174 571 311 Cuts at: 3473 3842 4519 4862 5913 5980 6936 7894 442 Size: 369 677 343 1051 67 956 958 1753 Fragments arranged by size: 1753 1174 1051 958 956 677 571 416 399 369 343 311 83 67 53 24 BmtI G_CTAG'C Cuts at: 1056 3014 1056 Size: 1958 7247 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4528 4792 6186 6421 4528 Size: 264 1394 235 7312 Fragments arranged by size: 7312 1394 264 235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2439 3728 3884 3984 4182 4406 4583 5859 Size: 1200 1289 156 100 198 224 177 1276 Cuts at: 5859 5984 6266 7865 1239 Size: 125 282 1599 2579 Fragments arranged by size: 2579 1599 1289 1276 1200 282 224 198 177 156 125 100 Bpu10I CC'TnA_GC Cuts at: 2622 4199 4678 2622 Size: 1577 479 7149 Fragments arranged by size: 7149 1577 479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5444 7330 8195 8493 5444 Size: 1886 865 298 6156 Fragments arranged by size: 6156 1886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3305 3707 3860 4158 7868 882 Size: 617 1806 402 153 298 3710 2219 Fragments arranged by size: 3710 2219 1806 617 402 298 153 BsaAI yAC'GTr Cuts at: 493 1204 4636 5263 6502 6996 493 Size: 711 3432 627 1239 494 2702 Fragments arranged by size: 3432 2702 1239 711 627 494 BsaBI GATnn'nnATC Cuts at: 9199 9199 Size: 9205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2221 4423 4620 5302 Size: 53 83 186 936 688 2202 197 682 Cuts at: 5302 7012 7394 8029 275 Size: 1710 382 635 1451 Fragments arranged by size: 2202 1710 1451 936 688 682 635 382 197 186 83 53 BsaWI w'CCGG_w Cuts at: 3562 3729 7638 8036 8310 8457 8868 3562 Size: 167 3909 398 274 147 411 3899 Fragments arranged by size: 3909 3899 411 398 274 167 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3459 3489 5090 5120 5267 Size: 30 165 30 2056 30 1601 30 147 Cuts at: 5267 5297 5308 5338 1178 Size: 30 11 30 5045 Fragments arranged by size: 5045 2056 1601 165 147 30 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2197 4256 4580 4583 4829 4894 5098 Size: 949 62 2059 324 3 246 65 204 Cuts at: 5098 5275 5417 5420 6200 1186 Size: 177 142 3 780 4191 Fragments arranged by size: 4191 2059 949 780 324 246 204 177 142 65 62 3 3 BseYI C'CCAG_C Cuts at: 2040 2112 3028 3130 3137 3206 3499 4804 5182 Size: 72 916 102 7 69 293 1305 378 Cuts at: 5182 5200 5728 5973 6145 8408 8904 2040 Size: 18 528 245 172 2263 496 2341 Fragments arranged by size: 2341 2263 1305 916 528 496 378 293 245 172 102 72 69 18 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2547 4301 4348 4948 5285 5558 5852 Size: 573 811 1754 47 600 337 273 294 Cuts at: 5852 1163 Size: 4516 Fragments arranged by size: 4516 1754 811 600 573 337 294 273 47 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6655 7416 8444 1540 Size: 275 186 4654 761 1028 2301 Fragments arranged by size: 4654 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2157 2745 3944 3972 4260 4580 4986 5373 Size: 33 588 1199 28 288 320 406 387 Cuts at: 5373 5901 7266 7351 8422 2124 Size: 528 1365 85 1071 2907 Fragments arranged by size: 2907 1365 1199 1071 588 528 406 387 320 288 85 33 28 BsmI GAATG_Cn' Cuts at: 3426 3663 5809 6716 6809 3426 Size: 237 2146 907 93 5822 Fragments arranged by size: 5822 2146 907 237 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2157 2177 2526 2623 2631 2745 Size: 399 334 33 20 349 97 8 114 Cuts at: 2745 2836 2931 3138 3472 3493 3612 3944 3972 Size: 91 95 207 334 21 119 332 28 Cuts at: 3972 4219 4260 4580 4821 4986 5190 5207 5373 Size: 247 41 320 241 165 204 17 166 Cuts at: 5373 5735 5901 5919 6012 6208 6524 7266 7351 Size: 362 166 18 93 196 316 742 85 Cuts at: 7351 8422 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 362 349 334 334 332 320 316 247 241 207 204 196 166 166 165 119 114 97 95 93 91 85 41 33 28 21 20 18 17 8 BspEI T'CCGG_A Cuts at: 8868 8868 Size: 9205 BspHI T'CATG_A Cuts at: 7094 8824 7094 Size: 1730 7475 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3265 3270 4320 5421 5703 6560 844 Size: 2421 5 1050 1101 282 857 3489 Fragments arranged by size: 3489 2421 1101 1050 857 282 5 BsrBI CCG'CTC Cuts at: 1394 1823 2071 5671 7092 1394 Size: 429 248 3600 1421 3507 Fragments arranged by size: 3600 3507 1421 429 248 BsrDI GCAATG_nn' Cuts at: 65 1248 2482 4941 6327 7700 7874 65 Size: 1183 1234 2459 1386 1373 174 1396 Fragments arranged by size: 2459 1396 1386 1373 1234 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3014 3562 5735 5787 7849 8036 1700 Size: 110 1204 548 2173 52 2062 187 2869 Fragments arranged by size: 2869 2173 2062 1204 548 187 110 52 BsrGI T'GTAC_A Cuts at: 96 4104 96 Size: 4008 5197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9205 BssSI C'ACGA_G Cuts at: 2607 2802 4233 7265 8277 2607 Size: 195 1431 3032 1012 3535 Fragments arranged by size: 3535 3032 1431 1012 195 BstAPI GCAn_nnn'nTGC Cuts at: 794 6454 794 Size: 5660 3545 BstBI TT'CG_AA Cuts at: 2007 8009 2007 Size: 6002 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9205 BstXI CCAn_nnnn'nTGG Cuts at: 5962 5962 Size: 9205 BstYI r'GATC_y Cuts at: 1972 3732 3787 4044 4749 5091 5981 6225 6535 Size: 1760 55 257 705 342 890 244 310 Cuts at: 6535 6902 7287 7304 8756 8842 8854 9200 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1760 1452 890 705 385 367 346 342 310 257 244 86 55 17 12 BstZ17I GTA'TAC Cuts at: 3544 3544 Size: 9205 Bsu36I CC'TnA_GG Cuts at: 2655 2754 2655 Size: 99 9106 BtgI C'CryG_G Cuts at: 513 1065 2022 2526 2725 2986 4623 4659 5082 Size: 552 957 504 199 261 1637 36 423 Cuts at: 5082 6123 513 Size: 1041 3595 Fragments arranged by size: 3595 1637 1041 957 552 504 423 261 199 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3943 5009 5512 487 Size: 683 56 2717 1066 503 4180 Fragments arranged by size: 4180 2717 1066 683 503 56 BtsI GCAGTG_nn' Cuts at: 2101 3311 3618 4002 4877 4986 5474 5511 5720 Size: 1210 307 384 875 109 488 37 209 Cuts at: 5720 6101 6721 7515 7535 2101 Size: 381 620 794 20 3771 Fragments arranged by size: 3771 1210 875 794 620 488 384 381 307 209 109 37 20 ClaI AT'CG_AT Cuts at: 1554 6237 1554 Size: 4683 4522 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9170 DraI TTT'AAA Cuts at: 6509 6509 Size: 9205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8212 809 Size: 507 6896 1802 Fragments arranged by size: 6896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2404 3246 Size: 54 1425 50 275 18 236 338 842 Cuts at: 3246 3357 4026 4281 4302 5163 5193 5343 5789 Size: 111 669 255 21 861 30 150 446 Cuts at: 5789 6198 6411 6652 7541 9170 8 Size: 409 213 241 889 1629 43 Fragments arranged by size: 1629 1425 889 861 842 669 446 409 338 275 255 241 236 213 150 111 54 50 43 30 21 18 EagI C'GGCC_G Cuts at: 1537 1812 6652 1537 Size: 275 4840 4090 Fragments arranged by size: 4840 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2958 3932 4694 4826 5673 5906 7135 1353 Size: 1605 974 762 132 847 233 1229 3423 Fragments arranged by size: 3423 1605 1229 974 847 762 233 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7792 8166 8312 387 Size: 1064 138 6203 374 146 1280 Fragments arranged by size: 6203 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2743 3942 3970 4258 2743 Size: 1199 28 288 7690 Fragments arranged by size: 7690 1199 288 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2439 2685 2915 3065 3728 3884 3984 Size: 393 807 246 230 150 663 156 100 Cuts at: 3984 4182 4406 4583 4781 5859 5984 6110 6266 Size: 198 224 177 198 1078 125 126 156 Cuts at: 6266 7268 7865 8652 8920 1239 Size: 1002 597 787 268 1524 Fragments arranged by size: 1524 1078 1002 807 787 663 597 393 268 246 230 224 198 198 177 156 156 150 126 125 100 EcoNI CCTnn'n_nnAGG Cuts at: 2639 2963 4595 5699 6062 6107 2639 Size: 324 1632 1104 363 45 5737 Fragments arranged by size: 5737 1632 1104 363 324 45 EcoO109I rG'GnC_Cy Cuts at: 2309 2417 2522 2730 2847 2859 2927 2928 3134 Size: 108 105 208 117 12 68 1 206 Cuts at: 3134 3160 3229 3844 5280 5551 6055 6072 6085 Size: 26 69 615 1436 271 504 17 13 Cuts at: 6085 6494 2309 Size: 409 5020 Fragments arranged by size: 5020 1436 615 504 409 271 208 206 117 108 105 69 68 26 17 13 12 1 EcoRI G'AATT_C Cuts at: 6514 6892 6514 Size: 378 8827 FspI TGC'GCA Cuts at: 6422 7711 6422 Size: 1289 7916 HaeII r_GCGC'y Cuts at: 1678 2130 2224 4426 4623 5272 5305 5517 8352 Size: 452 94 2202 197 649 33 212 2835 Cuts at: 8352 1678 Size: 2531 Fragments arranged by size: 2835 2531 2202 649 452 212 197 94 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2208 2240 3498 3530 3685 Size: 32 135 32 636 32 1258 32 155 Cuts at: 3685 3717 4056 4088 7846 7878 7920 7952 1373 Size: 32 339 32 3758 32 42 32 2626 Fragments arranged by size: 3758 2626 1258 636 339 155 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3331 6549 6795 669 Size: 988 281 1393 3218 246 3079 Fragments arranged by size: 3218 3079 1393 988 281 246 HindIII A'AGCT_T Cuts at: 748 3401 4644 748 Size: 2653 1243 5309 Fragments arranged by size: 5309 2653 1243 HpaI GTT'AAC Cuts at: 6795 6795 Size: 9205 KasI G'GCGC_C Cuts at: 2220 4422 4619 5301 2220 Size: 2202 197 682 6124 Fragments arranged by size: 6124 2202 682 197 KpnI G_GTAC'C Cuts at: 3326 6530 3326 Size: 3204 6001 MfeI C'AATT_G Cuts at: 6804 6804 Size: 9205 MluI A'CGCG_T Cuts at: 9081 9081 Size: 9205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2251 3832 8319 8503 1305 Size: 946 1581 4487 184 2007 Fragments arranged by size: 4487 2007 1581 946 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2406 3248 3359 4028 4304 Size: 54 1425 343 574 842 111 669 276 Cuts at: 4304 5165 6200 10 Size: 861 1035 3015 Fragments arranged by size: 3015 1425 1035 861 842 669 574 343 276 111 54 MslI CAynn'nnrTG Cuts at: 518 1166 2584 2839 3387 3629 4868 5657 6105 Size: 648 1418 255 548 242 1239 789 448 Cuts at: 6105 7163 7522 7681 518 Size: 1058 359 159 2042 Fragments arranged by size: 2042 1418 1239 1058 789 648 548 448 359 255 242 159 MspA1I CmG'CkG Cuts at: 1606 2044 2077 2116 2263 2348 2557 3130 4102 Size: 438 33 39 147 85 209 573 972 Cuts at: 4102 4286 4759 4834 5012 5728 5962 6187 6277 Size: 184 473 75 178 716 234 225 90 Cuts at: 6277 6451 6621 7298 8446 8691 1606 Size: 174 170 677 1148 245 2120 Fragments arranged by size: 2120 1148 972 716 677 573 473 438 245 234 225 209 184 178 174 170 147 90 85 75 39 33 NarI GG'CG_CC Cuts at: 2221 4423 4620 5302 2221 Size: 2202 197 682 6124 Fragments arranged by size: 6124 2202 682 197 NcoI C'CATG_G Cuts at: 513 1065 2022 2526 6123 513 Size: 552 957 504 3597 3595 Fragments arranged by size: 3597 3595 957 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 9205 NheI G'CTAG_C Cuts at: 1052 3010 1052 Size: 1958 7247 NotI GC'GGCC_GC Cuts at: 6652 6652 Size: 9205 NspI r_CATG'y Cuts at: 1132 1229 3992 4339 6563 8108 1132 Size: 97 2763 347 2224 1545 2229 Fragments arranged by size: 2763 2229 2224 1545 347 97 PasI CC'CwG_GG Cuts at: 1348 2519 2616 5564 5710 5749 6052 1348 Size: 1171 97 2948 146 39 303 4501 Fragments arranged by size: 4501 2948 1171 303 146 97 39 PciI A'CATG_T Cuts at: 8104 8104 Size: 9205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2371 4485 4672 5773 1108 Size: 1263 2114 187 1101 4540 Fragments arranged by size: 4540 2114 1263 1101 187 PfoI T'CCnGG_A Cuts at: 2212 2958 3705 5766 2212 Size: 746 747 2061 5651 Fragments arranged by size: 5651 2061 747 746 PmeI GTTT'AAAC Cuts at: 6509 6509 Size: 9205 PmlI CAC'GTG Cuts at: 4636 5263 4636 Size: 627 8578 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7274 7306 8787 8819 7274 Size: 32 1481 32 7660 Fragments arranged by size: 7660 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2309 2417 3844 6494 2309 Size: 108 1427 2650 5020 Fragments arranged by size: 5020 2650 1427 108 PshAI GACnn'nnGTC Cuts at: 1613 4504 1613 Size: 2891 6314 PsiI TTA'TAA Cuts at: 6775 6775 Size: 9205 PspOMI G'GGCC_C Cuts at: 2522 2927 3134 2522 Size: 405 207 8593 Fragments arranged by size: 8593 405 207 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3095 3127 3095 Size: 32 9173 PstI C_TGCA'G Cuts at: 830 2210 2556 2787 3045 3799 4599 5727 6459 Size: 1380 346 231 258 754 800 1128 732 Cuts at: 6459 6557 830 Size: 98 3478 Fragments arranged by size: 3478 1380 1128 800 754 732 346 258 231 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9205 PvuII CAG'CTG Cuts at: 2044 2116 2348 2557 3130 4102 4759 4834 5012 Size: 72 232 209 573 972 657 75 178 Cuts at: 5012 5728 5962 6187 6277 6451 2044 Size: 716 234 225 90 174 4798 Fragments arranged by size: 4798 972 716 657 573 234 232 225 209 178 174 90 75 72 SacI G_AGCT'C Cuts at: 2745 3944 3972 4260 2745 Size: 1199 28 288 7690 Fragments arranged by size: 7690 1199 288 28 SalI G'TCGA_C Cuts at: 1936 6547 1936 Size: 4611 4594 SanDI GG'GwC_CC Cuts at: 2417 3844 2417 Size: 1427 7778 SapI GCTCTTCn'nnn_ Cuts at: 3932 4826 5906 3932 Size: 894 1080 7231 Fragments arranged by size: 7231 1080 894 SbfI CC_TGCA'GG Cuts at: 6557 6557 Size: 9205 ScaI AGT'ACT Cuts at: 7453 9165 7453 Size: 1712 7493 SfcI C'TryA_G Cuts at: 826 1045 2206 2552 2783 3041 3377 3778 3795 Size: 219 1161 346 231 258 336 401 17 Cuts at: 3795 3807 4595 5275 5392 5486 5723 6455 6553 Size: 12 788 680 117 94 237 732 98 Cuts at: 6553 7688 8369 8560 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1161 1135 788 732 681 680 401 346 336 258 237 231 219 191 117 98 94 17 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2729 5384 2729 Size: 2655 6550 SfoI GGC'GCC Cuts at: 2222 4424 4621 5303 2222 Size: 2202 197 682 6124 Fragments arranged by size: 6124 2202 682 197 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9205 SmaI CCC'GGG Cuts at: 1872 2414 6532 1872 Size: 542 4118 4545 Fragments arranged by size: 4545 4118 542 SmlI C'TyrA_G Cuts at: 820 1017 1943 3492 5423 7309 8210 8472 820 Size: 197 926 1549 1931 1886 901 262 1553 Fragments arranged by size: 1931 1886 1553 1549 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6502 6996 493 Size: 6009 494 2702 Fragments arranged by size: 6009 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9205 SphI G_CATG'C Cuts at: 1132 4339 6563 1132 Size: 3207 2224 3774 Fragments arranged by size: 3774 3207 2224 SspI AAT'ATT Cuts at: 5 52 7129 5 Size: 47 7077 2081 Fragments arranged by size: 7077 2081 47 StuI AGG'CCT Cuts at: 1517 3482 3773 5178 1517 Size: 1965 291 1405 5544 Fragments arranged by size: 5544 1965 1405 291 StyI C'CwwG_G Cuts at: 513 1065 2022 2421 2427 2526 3627 3686 3723 Size: 552 957 399 6 99 1101 59 37 Cuts at: 3723 4011 5352 6123 6644 513 Size: 288 1341 771 521 3074 Fragments arranged by size: 3074 1341 1101 957 771 552 521 399 288 99 59 37 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 2371 3020 3284 3679 4046 4382 Size: 1255 546 245 649 264 395 367 336 Cuts at: 4382 5095 6116 6137 7232 7249 7402 7587 8032 Size: 713 1021 21 1095 17 153 185 445 Cuts at: 8032 325 Size: 1498 Fragments arranged by size: 1498 1255 1095 1021 713 649 546 445 395 367 336 264 245 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3111 4104 4531 Size: 274 80 33 51 1443 1134 993 427 Cuts at: 4531 5253 5682 7451 9163 96 Size: 722 429 1769 1712 138 Fragments arranged by size: 1769 1712 1443 1134 993 722 429 427 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5308 5340 9165 9197 5308 Size: 32 3825 32 5316 Fragments arranged by size: 5316 3825 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9205 XbaI T'CTAG_A Cuts at: 6541 6541 Size: 9205 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2613 3352 3721 3980 6130 6253 2613 Size: 739 369 259 2150 123 5565 Fragments arranged by size: 5565 2150 739 369 259 123 XhoI C'TCGA_G Cuts at: 1943 3492 1943 Size: 1549 7656 XmaI C'CCGG_G Cuts at: 1870 2412 6530 1870 Size: 542 4118 4545 Fragments arranged by size: 4545 4118 542 XmnI GAAnn'nnTTC Cuts at: 2031 7334 2031 Size: 5303 3902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7013 276 Size: 53 83 186 936 5479 2468 Fragments arranged by size: 5479 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbvCI BfrBI BsiWI BsmBI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacII SexAI SrfI SwaI