Restriction map for pFN21ASDF0002
 (Circular) (Six-base) MAPSORT of: pFN21ASDF0002.seq  Check: 2666  from: 1  to: 9205



pFN21ASDF0002  9205 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   4320    4320 
   Size:       9205
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    7015     278 
   Size:         53      83     186     936    5479    2468

  Fragments arranged by size: 

                5479    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3543    4242    6548    1937 
   Size:       1606     699    2306    4594

  Fragments arranged by size: 

                4594    2306    1606     699
 
Acc65I G'GTAC_C

Cuts at:   3322    6526    3322 
   Size:       3204    6001
 
AclI AA'CG_TT

Cuts at:   1563    7332    7705    1563 
   Size:       5769     373    3063

  Fragments arranged by size: 

                5769    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2685    2915    3065    4781    6110    7268    8652    8920 
   Size:       1053     230     150    1716    1329    1158    1384     268

Cuts at:   8920    1632 
   Size:       1917

  Fragments arranged by size: 

                1917    1716    1384    1329    1158    1053     268     230
                 150
 
AfeI AGC'GCT

Cuts at:   5515    5515 
   Size:       9205
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    9008
 
AflIII A'CryG_T

Cuts at:   1203    4635    8104    9081    1203 
   Size:       3432    3469     977    1327

  Fragments arranged by size: 

                3469    3432    1327     977
 
AgeI A'CCGG_T

Cuts at:   3562    8036    3562 
   Size:       4474    4731
 
AhdI GACnn_n'nnGTC

Cuts at:   3512    7934    3512 
   Size:       4422    4783
 
AleI CACnn'nnGTG

Cuts at:   1166    2839    3629    6105    1166 
   Size:       1673     790    2476    4266

  Fragments arranged by size: 

                4266    2476    1673     790
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2519    2577    3405    1184 
   Size:       1335      58     828    6984

  Fragments arranged by size: 

                6984    1335     828      58
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4029    4061    4029 
   Size:         32    9173
 
AlwNI CAG_nnn'CTG

Cuts at:   2163    2597    3463    5473    5722    6088    6451    6454    6948 
   Size:        434     866    2010     249     366     363       3     494

Cuts at:   6948    8520    2163 
   Size:       1572    2848

  Fragments arranged by size: 

                2848    2010    1572     866     494     434     366     363
                 249       3
 
ApaI G_GGCC'C

Cuts at:   2526    2931    3138    2526 
   Size:        405     207    8593

  Fragments arranged by size: 

                8593     405     207
 
ApaLI G'TGCA_C

Cuts at:   7262    8418    7262 
   Size:       1156    8049
 
ApoI r'AATT_y

Cuts at:   1493    4954    6514    6740    6892    9001    1493 
   Size:       3461    1560     226     152    2109    1697

  Fragments arranged by size: 

                3461    2109    1697    1560     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    7759     160 
   Size:        872    6727    1606

  Fragments arranged by size: 

                6727    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9205
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2179    2412    2639    3471    3492    5617    6530 
   Size:         73     236     233     227     832      21    2125     913

Cuts at:   6530    1870 
   Size:       4545

  Fragments arranged by size: 

                4545    2125     913     832     236     233     227      73
                  21
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   4132    4165    4132 
   Size:         33    9172
 
BamHI G'GATC_C

Cuts at:   3732    4044    5091    6535    6902    3732 
   Size:        312    1047    1444     367    6035

  Fragments arranged by size: 

                6035    1444    1047     367     312
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2174    2220    2710    3322    3609 
   Size:        325     217     627     387      46     490     612     287

Cuts at:   3609    4422    4619    4997    5053    5301    5439    5732    6526 
   Size:        813     197     378      56     248     138     293     794

Cuts at:   6526    7981     618 
   Size:       1455    1842

  Fragments arranged by size: 

                1842    1455     813     794     627     612     490     387
                 378     325     293     287     248     217     197     138
                  56      46
 
BanII G_rGCy'C

Cuts at:   1391    2526    2623    2631    2745    2931    3138    3472    3493 
   Size:       1135      97       8     114     186     207     334      21

Cuts at:   3493    3944    3972    4260    4821    5190    5207    5919    6012 
   Size:        451      28     288     561     369      17     712      93

Cuts at:   6012    6208    6524    1391 
   Size:        196     316    4072

  Fragments arranged by size: 

                4072    1135     712     561     451     369     334     316
                 288     207     196     186     114      97      93      28
                  21      17       8
 
BbeI G_GCGC'C

Cuts at:   2224    4426    4623    5305    2224 
   Size:       2202     197     682    6124

  Fragments arranged by size: 

                6124    2202     682     197
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2248    2800    4091    5874    6272     928 
   Size:        971     349     552    1291    1783     398    3861

  Fragments arranged by size: 

                3861    1783    1291     971     552     398     349
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    7396    7430    1903 
   Size:         34    5459      34    3678

  Fragments arranged by size: 

                5459    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4160    7097    8307    4160 
   Size:       2937    1210    5058

  Fragments arranged by size: 

                5058    2937    1210
 
BclI T'GATC_A

Cuts at:   1548    3693    1548 
   Size:       2145    7060
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2493    2708    2729 
   Size:        107     122      71     631     605     821     215      21

Cuts at:   2729    3020    3488    5171    5384    5868    7816     136 
   Size:        291     468    1683     213     484    1948    1525

  Fragments arranged by size: 

                1948    1683    1525     821     631     605     484     468
                 291     215     213     122     107      71      21
 
BglII A'GATC_T

Cuts at:   9200    9200 
   Size:       9205
 
BlpI GC'TnA_GC

Cuts at:   2123    4340    6622    2123 
   Size:       2217    2282    4706

  Fragments arranged by size: 

                4706    2282    2217
 
Bme1580I G_kGCm'C

Cuts at:   1790    2177    2526    2836    2931    3138    3612    4219    5735 
   Size:        387     349     310      95     207     474     607    1516

Cuts at:   5735    7266    8422    1790 
   Size:       1531    1156    2573

  Fragments arranged by size: 

                2573    1531    1516    1156     607     474     387     349
                 310     207      95
 
BmgBI CAC'GTC

Cuts at:   1318    5773    1318 
   Size:       4455    4750
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2591    3162    3473 
   Size:        416      53      83     399      24    1174     571     311

Cuts at:   3473    3842    4519    4862    5913    5980    6936    7894     442 
   Size:        369     677     343    1051      67     956     958    1753

  Fragments arranged by size: 

                1753    1174    1051     958     956     677     571     416
                 399     369     343     311      83      67      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    3014    1056 
   Size:       1958    7247
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4528    4792    6186    6421    4528 
   Size:        264    1394     235    7312

  Fragments arranged by size: 

                7312    1394     264     235
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2439    3728    3884    3984    4182    4406    4583    5859 
   Size:       1200    1289     156     100     198     224     177    1276

Cuts at:   5859    5984    6266    7865    1239 
   Size:        125     282    1599    2579

  Fragments arranged by size: 

                2579    1599    1289    1276    1200     282     224     198
                 177     156     125     100
 
Bpu10I CC'TnA_GC

Cuts at:   2622    4199    4678    2622 
   Size:       1577     479    7149

  Fragments arranged by size: 

                7149    1577     479
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   5444    7330    8195    8493    5444 
   Size:       1886     865     298    6156

  Fragments arranged by size: 

                6156    1886     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3305    3707    3860    4158    7868     882 
   Size:        617    1806     402     153     298    3710    2219

  Fragments arranged by size: 

                3710    2219    1806     617     402     298     153
 
BsaAI yAC'GTr

Cuts at:    493    1204    4636    5263    6502    6996     493 
   Size:        711    3432     627    1239     494    2702

  Fragments arranged by size: 

                3432    2702    1239     711     627     494
 
BsaBI GATnn'nnATC

Cuts at:   9199    9199 
   Size:       9205
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2221    4423    4620    5302 
   Size:         53      83     186     936     688    2202     197     682

Cuts at:   5302    7012    7394    8029     275 
   Size:       1710     382     635    1451

  Fragments arranged by size: 

                2202    1710    1451     936     688     682     635     382
                 197     186      83      53
 
BsaWI w'CCGG_w

Cuts at:   3562    3729    7638    8036    8310    8457    8868    3562 
   Size:        167    3909     398     274     147     411    3899

  Fragments arranged by size: 

                3909    3899     411     398     274     167     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3459    3489    5090    5120    5267 
   Size:         30     165      30    2056      30    1601      30     147

Cuts at:   5267    5297    5308    5338    1178 
   Size:         30      11      30    5045

  Fragments arranged by size: 

                5045    2056    1601     165     147      30      30      30
                  30      30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2135    2197    4256    4580    4583    4829    4894    5098 
   Size:        949      62    2059     324       3     246      65     204

Cuts at:   5098    5275    5417    5420    6200    1186 
   Size:        177     142       3     780    4191

  Fragments arranged by size: 

                4191    2059     949     780     324     246     204     177
                 142      65      62       3       3
 
BseYI C'CCAG_C

Cuts at:   2040    2112    3028    3130    3137    3206    3499    4804    5182 
   Size:         72     916     102       7      69     293    1305     378

Cuts at:   5182    5200    5728    5973    6145    8408    8904    2040 
   Size:         18     528     245     172    2263     496    2341

  Fragments arranged by size: 

                2341    2263    1305     916     528     496     378     293
                 245     172     102      72      69      18       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2547    4301    4348    4948    5285    5558    5852 
   Size:        573     811    1754      47     600     337     273     294

Cuts at:   5852    1163 
   Size:       4516

  Fragments arranged by size: 

                4516    1754     811     600     573     337     294     273
                  47
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    6655    7416    8444    1540 
   Size:        275     186    4654     761    1028    2301

  Fragments arranged by size: 

                4654    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2124    2157    2745    3944    3972    4260    4580    4986    5373 
   Size:         33     588    1199      28     288     320     406     387

Cuts at:   5373    5901    7266    7351    8422    2124 
   Size:        528    1365      85    1071    2907

  Fragments arranged by size: 

                2907    1365    1199    1071     588     528     406     387
                 320     288      85      33      28
 
BsmI GAATG_Cn'

Cuts at:   3426    3663    5809    6716    6809    3426 
   Size:        237    2146     907      93    5822

  Fragments arranged by size: 

                5822    2146     907     237      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2124    2157    2177    2526    2623    2631    2745 
   Size:        399     334      33      20     349      97       8     114

Cuts at:   2745    2836    2931    3138    3472    3493    3612    3944    3972 
   Size:         91      95     207     334      21     119     332      28

Cuts at:   3972    4219    4260    4580    4821    4986    5190    5207    5373 
   Size:        247      41     320     241     165     204      17     166

Cuts at:   5373    5735    5901    5919    6012    6208    6524    7266    7351 
   Size:        362     166      18      93     196     316     742      85

Cuts at:   7351    8422    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     362     349     334     334
                 332     320     316     247     241     207     204     196
                 166     166     165     119     114      97      95      93
                  91      85      41      33      28      21      20      18
                  17       8
 
BspEI T'CCGG_A

Cuts at:   8868    8868 
   Size:       9205
 
BspHI T'CATG_A

Cuts at:   7094    8824    7094 
   Size:       1730    7475
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3265    3270    4320    5421    5703    6560     844 
   Size:       2421       5    1050    1101     282     857    3489

  Fragments arranged by size: 

                3489    2421    1101    1050     857     282       5
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2071    5671    7092    1394 
   Size:        429     248    3600    1421    3507

  Fragments arranged by size: 

                3600    3507    1421     429     248
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2482    4941    6327    7700    7874      65 
   Size:       1183    1234    2459    1386    1373     174    1396

  Fragments arranged by size: 

                2459    1396    1386    1373    1234    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3014    3562    5735    5787    7849    8036    1700 
   Size:        110    1204     548    2173      52    2062     187    2869

  Fragments arranged by size: 

                2869    2173    2062    1204     548     187     110      52
            
 
BsrGI T'GTAC_A

Cuts at:     96    4104      96 
   Size:       4008    5197
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       9205
 
BssSI C'ACGA_G

Cuts at:   2607    2802    4233    7265    8277    2607 
   Size:        195    1431    3032    1012    3535

  Fragments arranged by size: 

                3535    3032    1431    1012     195
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    6454     794 
   Size:       5660    3545
 
BstBI TT'CG_AA

Cuts at:   2007    8009    2007 
   Size:       6002    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       9205
 
BstXI CCAn_nnnn'nTGG

Cuts at:   5962    5962 
   Size:       9205
 
BstYI r'GATC_y

Cuts at:   1972    3732    3787    4044    4749    5091    5981    6225    6535 
   Size:       1760      55     257     705     342     890     244     310

Cuts at:   6535    6902    7287    7304    8756    8842    8854    9200    1972 
   Size:        367     385      17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1760    1452     890     705     385     367     346
                 342     310     257     244      86      55      17      12
            
 
BstZ17I GTA'TAC

Cuts at:   3544    3544 
   Size:       9205
 
Bsu36I CC'TnA_GG

Cuts at:   2655    2754    2655 
   Size:         99    9106
 
BtgI C'CryG_G

Cuts at:    513    1065    2022    2526    2725    2986    4623    4659    5082 
   Size:        552     957     504     199     261    1637      36     423

Cuts at:   5082    6123     513 
   Size:       1041    3595

  Fragments arranged by size: 

                3595    1637    1041     957     552     504     423     261
                 199      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3943    5009    5512     487 
   Size:        683      56    2717    1066     503    4180

  Fragments arranged by size: 

                4180    2717    1066     683     503      56
 
BtsI GCAGTG_nn'

Cuts at:   2101    3311    3618    4002    4877    4986    5474    5511    5720 
   Size:       1210     307     384     875     109     488      37     209

Cuts at:   5720    6101    6721    7515    7535    2101 
   Size:        381     620     794      20    3771

  Fragments arranged by size: 

                3771    1210     875     794     620     488     384     381
                 307     209     109      37      20
 
ClaI AT'CG_AT

Cuts at:   1554    6237    1554 
   Size:       4683    4522
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    9170
 
DraI TTT'AAA

Cuts at:   6509    6509 
   Size:       9205
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    8212     809 
   Size:        507    6896    1802

  Fragments arranged by size: 

                6896    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2066    2404    3246 
   Size:         54    1425      50     275      18     236     338     842

Cuts at:   3246    3357    4026    4281    4302    5163    5193    5343    5789 
   Size:        111     669     255      21     861      30     150     446

Cuts at:   5789    6198    6411    6652    7541    9170       8 
   Size:        409     213     241     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     861     842     669     446     409
                 338     275     255     241     236     213     150     111
                  54      50      43      30      21      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    6652    1537 
   Size:        275    4840    4090

  Fragments arranged by size: 

                4840    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2958    3932    4694    4826    5673    5906    7135    1353 
   Size:       1605     974     762     132     847     233    1229    3423

  Fragments arranged by size: 

                3423    1605    1229     974     847     762     233     132
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    7792    8166    8312     387 
   Size:       1064     138    6203     374     146    1280

  Fragments arranged by size: 

                6203    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2743    3942    3970    4258    2743 
   Size:       1199      28     288    7690

  Fragments arranged by size: 

                7690    1199     288      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2439    2685    2915    3065    3728    3884    3984 
   Size:        393     807     246     230     150     663     156     100

Cuts at:   3984    4182    4406    4583    4781    5859    5984    6110    6266 
   Size:        198     224     177     198    1078     125     126     156

Cuts at:   6266    7268    7865    8652    8920    1239 
   Size:       1002     597     787     268    1524

  Fragments arranged by size: 

                1524    1078    1002     807     787     663     597     393
                 268     246     230     224     198     198     177     156
                 156     150     126     125     100
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2639    2963    4595    5699    6062    6107    2639 
   Size:        324    1632    1104     363      45    5737

  Fragments arranged by size: 

                5737    1632    1104     363     324      45
 
EcoO109I rG'GnC_Cy

Cuts at:   2309    2417    2522    2730    2847    2859    2927    2928    3134 
   Size:        108     105     208     117      12      68       1     206

Cuts at:   3134    3160    3229    3844    5280    5551    6055    6072    6085 
   Size:         26      69     615    1436     271     504      17      13

Cuts at:   6085    6494    2309 
   Size:        409    5020

  Fragments arranged by size: 

                5020    1436     615     504     409     271     208     206
                 117     108     105      69      68      26      17      13
                  12       1
 
EcoRI G'AATT_C

Cuts at:   6514    6892    6514 
   Size:        378    8827
 
FspI TGC'GCA

Cuts at:   6422    7711    6422 
   Size:       1289    7916
 
HaeII r_GCGC'y

Cuts at:   1678    2130    2224    4426    4623    5272    5305    5517    8352 
   Size:        452      94    2202     197     649      33     212    2835

Cuts at:   8352    1678 
   Size:       2531

  Fragments arranged by size: 

                2835    2531    2202     649     452     212     197      94
                  33
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2208    2240    3498    3530    3685 
   Size:         32     135      32     636      32    1258      32     155

Cuts at:   3685    3717    4056    4088    7846    7878    7920    7952    1373 
   Size:         32     339      32    3758      32      42      32    2626

  Fragments arranged by size: 

                3758    2626    1258     636     339     155     135      42
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3331    6549    6795     669 
   Size:        988     281    1393    3218     246    3079

  Fragments arranged by size: 

                3218    3079    1393     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3401    4644     748 
   Size:       2653    1243    5309

  Fragments arranged by size: 

                5309    2653    1243
 
HpaI GTT'AAC

Cuts at:   6795    6795 
   Size:       9205
 
KasI G'GCGC_C

Cuts at:   2220    4422    4619    5301    2220 
   Size:       2202     197     682    6124

  Fragments arranged by size: 

                6124    2202     682     197
 
KpnI G_GTAC'C

Cuts at:   3326    6530    3326 
   Size:       3204    6001
 
MfeI C'AATT_G

Cuts at:   6804    6804 
   Size:       9205
 
MluI A'CGCG_T

Cuts at:   9081    9081 
   Size:       9205
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2251    3832    8319    8503    1305 
   Size:        946    1581    4487     184    2007

  Fragments arranged by size: 

                4487    2007    1581     946     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2406    3248    3359    4028    4304 
   Size:         54    1425     343     574     842     111     669     276

Cuts at:   4304    5165    6200      10 
   Size:        861    1035    3015

  Fragments arranged by size: 

                3015    1425    1035     861     842     669     574     343
                 276     111      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2584    2839    3387    3629    4868    5657    6105 
   Size:        648    1418     255     548     242    1239     789     448

Cuts at:   6105    7163    7522    7681     518 
   Size:       1058     359     159    2042

  Fragments arranged by size: 

                2042    1418    1239    1058     789     648     548     448
                 359     255     242     159
 
MspA1I CmG'CkG

Cuts at:   1606    2044    2077    2116    2263    2348    2557    3130    4102 
   Size:        438      33      39     147      85     209     573     972

Cuts at:   4102    4286    4759    4834    5012    5728    5962    6187    6277 
   Size:        184     473      75     178     716     234     225      90

Cuts at:   6277    6451    6621    7298    8446    8691    1606 
   Size:        174     170     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     972     716     677     573     473     438
                 245     234     225     209     184     178     174     170
                 147      90      85      75      39      33
 
NarI GG'CG_CC

Cuts at:   2221    4423    4620    5302    2221 
   Size:       2202     197     682    6124

  Fragments arranged by size: 

                6124    2202     682     197
 
NcoI C'CATG_G

Cuts at:    513    1065    2022    2526    6123     513 
   Size:        552     957     504    3597    3595

  Fragments arranged by size: 

                3597    3595     957     552     504
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       9205
 
NheI G'CTAG_C

Cuts at:   1052    3010    1052 
   Size:       1958    7247
 
NotI GC'GGCC_GC

Cuts at:   6652    6652 
   Size:       9205
 
NspI r_CATG'y

Cuts at:   1132    1229    3992    4339    6563    8108    1132 
   Size:         97    2763     347    2224    1545    2229

  Fragments arranged by size: 

                2763    2229    2224    1545     347      97
 
PasI CC'CwG_GG

Cuts at:   1348    2519    2616    5564    5710    5749    6052    1348 
   Size:       1171      97    2948     146      39     303    4501

  Fragments arranged by size: 

                4501    2948    1171     303     146      97      39
 
PciI A'CATG_T

Cuts at:   8104    8104 
   Size:       9205
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2371    4485    4672    5773    1108 
   Size:       1263    2114     187    1101    4540

  Fragments arranged by size: 

                4540    2114    1263    1101     187
 
PfoI T'CCnGG_A

Cuts at:   2212    2958    3705    5766    2212 
   Size:        746     747    2061    5651

  Fragments arranged by size: 

                5651    2061     747     746
 
PmeI GTTT'AAAC

Cuts at:   6509    6509 
   Size:       9205
 
PmlI CAC'GTG

Cuts at:   4636    5263    4636 
   Size:        627    8578
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7274    7306    8787    8819    7274 
   Size:         32    1481      32    7660

  Fragments arranged by size: 

                7660    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2309    2417    3844    6494    2309 
   Size:        108    1427    2650    5020

  Fragments arranged by size: 

                5020    2650    1427     108
 
PshAI GACnn'nnGTC

Cuts at:   1613    4504    1613 
   Size:       2891    6314
 
PsiI TTA'TAA

Cuts at:   6775    6775 
   Size:       9205
 
PspOMI G'GGCC_C

Cuts at:   2522    2927    3134    2522 
   Size:        405     207    8593

  Fragments arranged by size: 

                8593     405     207
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   3095    3127    3095 
   Size:         32    9173
 
PstI C_TGCA'G

Cuts at:    830    2210    2556    2787    3045    3799    4599    5727    6459 
   Size:       1380     346     231     258     754     800    1128     732

Cuts at:   6459    6557     830 
   Size:         98    3478

  Fragments arranged by size: 

                3478    1380    1128     800     754     732     346     258
                 231      98
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9205
 
PvuII CAG'CTG

Cuts at:   2044    2116    2348    2557    3130    4102    4759    4834    5012 
   Size:         72     232     209     573     972     657      75     178

Cuts at:   5012    5728    5962    6187    6277    6451    2044 
   Size:        716     234     225      90     174    4798

  Fragments arranged by size: 

                4798     972     716     657     573     234     232     225
                 209     178     174      90      75      72
 
SacI G_AGCT'C

Cuts at:   2745    3944    3972    4260    2745 
   Size:       1199      28     288    7690

  Fragments arranged by size: 

                7690    1199     288      28
 
SalI G'TCGA_C

Cuts at:   1936    6547    1936 
   Size:       4611    4594
 
SanDI GG'GwC_CC

Cuts at:   2417    3844    2417 
   Size:       1427    7778
 
SapI GCTCTTCn'nnn_

Cuts at:   3932    4826    5906    3932 
   Size:        894    1080    7231

  Fragments arranged by size: 

                7231    1080     894
 
SbfI CC_TGCA'GG

Cuts at:   6557    6557 
   Size:       9205
 
ScaI AGT'ACT

Cuts at:   7453    9165    7453 
   Size:       1712    7493
 
SfcI C'TryA_G

Cuts at:    826    1045    2206    2552    2783    3041    3377    3778    3795 
   Size:        219    1161     346     231     258     336     401      17

Cuts at:   3795    3807    4595    5275    5392    5486    5723    6455    6553 
   Size:         12     788     680     117      94     237     732      98

Cuts at:   6553    7688    8369    8560     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1471    1161    1135     788     732     681     680     401
                 346     336     258     237     231     219     191     117
                  98      94      17      12
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2729    5384    2729 
   Size:       2655    6550
 
SfoI GGC'GCC

Cuts at:   2222    4424    4621    5303    2222 
   Size:       2202     197     682    6124

  Fragments arranged by size: 

                6124    2202     682     197
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9205
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       9205
 
SmaI CCC'GGG

Cuts at:   1872    2414    6532    1872 
   Size:        542    4118    4545

  Fragments arranged by size: 

                4545    4118     542
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3492    5423    7309    8210    8472     820 
   Size:        197     926    1549    1931    1886     901     262    1553

  Fragments arranged by size: 

                1931    1886    1553    1549     926     901     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    6502    6996     493 
   Size:       6009     494    2702

  Fragments arranged by size: 

                6009    2702     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9205
 
SphI G_CATG'C

Cuts at:   1132    4339    6563    1132 
   Size:       3207    2224    3774

  Fragments arranged by size: 

                3774    3207    2224
 
SspI AAT'ATT

Cuts at:      5      52    7129       5 
   Size:         47    7077    2081

  Fragments arranged by size: 

                7077    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3482    3773    5178    1517 
   Size:       1965     291    1405    5544

  Fragments arranged by size: 

                5544    1965    1405     291
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    2421    2427    2526    3627    3686    3723 
   Size:        552     957     399       6      99    1101      59      37

Cuts at:   3723    4011    5352    6123    6644     513 
   Size:        288    1341     771     521    3074

  Fragments arranged by size: 

                3074    1341    1101     957     771     552     521     399
                 288      99      59      37       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2126    2371    3020    3284    3679    4046    4382 
   Size:       1255     546     245     649     264     395     367     336

Cuts at:   4382    5095    6116    6137    7232    7249    7402    7587    8032 
   Size:        713    1021      21    1095      17     153     185     445

Cuts at:   8032     325 
   Size:       1498

  Fragments arranged by size: 

                1498    1255    1095    1021     713     649     546     445
                 395     367     336     264     245     185     153      21
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3111    4104    4531 
   Size:        274      80      33      51    1443    1134     993     427

Cuts at:   4531    5253    5682    7451    9163      96 
   Size:        722     429    1769    1712     138

  Fragments arranged by size: 

                1769    1712    1443    1134     993     722     429     427
                 274     138      80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5308    5340    9165    9197    5308 
   Size:         32    3825      32    5316

  Fragments arranged by size: 

                5316    3825      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       9205
 
XbaI T'CTAG_A

Cuts at:   6541    6541 
   Size:       9205
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2613    3352    3721    3980    6130    6253    2613 
   Size:        739     369     259    2150     123    5565

  Fragments arranged by size: 

                5565    2150     739     369     259     123
 
XhoI C'TCGA_G

Cuts at:   1943    3492    1943 
   Size:       1549    7656
 
XmaI C'CCGG_G

Cuts at:   1870    2412    6530    1870 
   Size:        542    4118    4545

  Fragments arranged by size: 

                4545    4118     542
 
XmnI GAAnn'nnTTC

Cuts at:   2031    7334    2031 
   Size:       5303    3902
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    7013     276 
   Size:         53      83     186     936    5479    2468

  Fragments arranged by size: 

                5479    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI     NheI
     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI
    PvuII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII    BbvCI    BfrBI    BsiWI    BsmBI   DraIII    EcoRV
     FalI     FseI    FspAI     NaeI   NgoMIV     NruI     NsiI     PacI
    PspXI    RsrII    SacII    SexAI     SrfI     SwaI
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