Restriction map for
pFN21ASDF0002
(Linear) (Six-base) MAPSORT of: FLJ00002.seq Check: 5719 from: 1 to: 4476
FLJ00002.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2297 4476
Size: 2297 2179
AccI GT'mk_AC
Cuts at: 0 1520 2219 4476
Size: 1520 699 2257
Fragments arranged by size:
2257 1520 699
Acc65I G'GTAC_C
Cuts at: 0 1299 4476
Size: 1299 3177
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 662 892 1042 2758 4087 4476
Size: 662 230 150 1716 1329 389
Fragments arranged by size:
1716 1329 662 389 230 150
AfeI AGC'GCT
Cuts at: 0 3492 4476
Size: 3492 984
AflIII A'CryG_T
Cuts at: 0 2612 4476
Size: 2612 1864
AgeI A'CCGG_T
Cuts at: 0 1539 4476
Size: 1539 2937
AhdI GACnn_n'nnGTC
Cuts at: 0 1489 4476
Size: 1489 2987
AleI CACnn'nnGTG
Cuts at: 0 816 1606 4082 4476
Size: 816 790 2476 394
Fragments arranged by size:
2476 816 790 394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 496 554 1382 4476
Size: 496 58 828 3094
Fragments arranged by size:
3094 828 496 58
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2006 2038 4476
Size: 2006 32 2438
Fragments arranged by size:
2438 2006 32
AlwNI CAG_nnn'CTG
Cuts at: 0 140 574 1440 3450 3699 4065 4428 4431
Size: 140 434 866 2010 249 366 363 3
Cuts at: 4431 4476
Size: 45
Fragments arranged by size:
2010 866 434 366 363 249 140 45
3
ApaI G_GGCC'C
Cuts at: 0 503 908 1115 4476
Size: 503 405 207 3361
Fragments arranged by size:
3361 503 405 207
ApoI r'AATT_y
Cuts at: 0 2931 4476
Size: 2931 1545
AvaI C'yCGr_G
Cuts at: 0 156 389 616 1448 1469 3594 4476
Size: 156 233 227 832 21 2125 882
Fragments arranged by size:
2125 882 832 233 227 156 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2109 2142 4476
Size: 2109 33 2334
Fragments arranged by size:
2334 2109 33
BamHI G'GATC_C
Cuts at: 0 1709 2021 3068 4476
Size: 1709 312 1047 1408
Fragments arranged by size:
1709 1408 1047 312
BanI G'GyrC_C
Cuts at: 0 151 197 687 1299 1586 2399 2596 2974
Size: 151 46 490 612 287 813 197 378
Cuts at: 2974 3030 3278 3416 3709 4476
Size: 56 248 138 293 767
Fragments arranged by size:
813 767 612 490 378 293 287 248
197 151 138 56 46
BanII G_rGCy'C
Cuts at: 0 503 600 608 722 908 1115 1449 1470
Size: 503 97 8 114 186 207 334 21
Cuts at: 1470 1921 1949 2237 2798 3167 3184 3896 3989
Size: 451 28 288 561 369 17 712 93
Cuts at: 3989 4185 4476
Size: 196 291
Fragments arranged by size:
712 561 503 451 369 334 291 288
207 196 186 114 97 93 28 21
17 8
BbeI G_GCGC'C
Cuts at: 0 201 2403 2600 3282 4476
Size: 201 2202 197 682 1194
Fragments arranged by size:
2202 1194 682 201 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 225 777 2068 3851 4249 4476
Size: 225 552 1291 1783 398 227
Fragments arranged by size:
1783 1291 552 398 227 225
BciVI GTATCCnnnnn_n'
Cuts at: 0 2137 4476
Size: 2137 2339
BclI T'GATC_A
Cuts at: 0 1670 4476
Size: 1670 2806
BglI GCCn_nnn'nGGC
Cuts at: 0 470 685 706 997 1465 3148 3361 3845
Size: 470 215 21 291 468 1683 213 484
Cuts at: 3845 4476
Size: 631
Fragments arranged by size:
1683 631 484 470 468 291 215 213
21
BlpI GC'TnA_GC
Cuts at: 0 100 2317 4476
Size: 100 2217 2159
Fragments arranged by size:
2217 2159 100
Bme1580I G_kGCm'C
Cuts at: 0 154 503 813 908 1115 1589 2196 3712
Size: 154 349 310 95 207 474 607 1516
Cuts at: 3712 4476
Size: 764
Fragments arranged by size:
1516 764 607 474 349 310 207 154
95
BmgBI CAC'GTC
Cuts at: 0 3750 4476
Size: 3750 726
BmrI ACTGGGnnnn_n'
Cuts at: 0 568 1139 1450 1819 2496 2839 3890 3957
Size: 568 571 311 369 677 343 1051 67
Cuts at: 3957 4476
Size: 519
Fragments arranged by size:
1051 677 571 568 519 369 343 311
67
BmtI G_CTAG'C
Cuts at: 0 991 4476
Size: 991 3485
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2505 2769 4163 4398 4476
Size: 2505 264 1394 235 78
Fragments arranged by size:
2505 1394 264 235 78
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1705 1861 1961 2159 2383 2560 3836
Size: 416 1289 156 100 198 224 177 1276
Cuts at: 3836 3961 4243 4476
Size: 125 282 233
Fragments arranged by size:
1289 1276 416 282 233 224 198 177
156 125 100
Bpu10I CC'TnA_GC
Cuts at: 0 599 2176 2655 4476
Size: 599 1577 479 1821
Fragments arranged by size:
1821 1577 599 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3421 4476
Size: 3421 1055
BsaI GGTCTCn'nnnn_
Cuts at: 0 1282 1684 1837 2135 4476
Size: 1282 402 153 298 2341
Fragments arranged by size:
2341 1282 402 298 153
BsaAI yAC'GTr
Cuts at: 0 2613 3240 4476
Size: 2613 627 1236
Fragments arranged by size:
2613 1236 627
BsaHI Gr'CG_yC
Cuts at: 0 198 2400 2597 3279 4476
Size: 198 2202 197 682 1197
Fragments arranged by size:
2202 1197 682 198 197
BsaWI w'CCGG_w
Cuts at: 0 1539 1706 4476
Size: 1539 167 2770
Fragments arranged by size:
2770 1539 167
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1436 1466 3067 3097 3244 3274 3285 3315
Size: 1436 30 1601 30 147 30 11 30
Cuts at: 3315 4476
Size: 1161
Fragments arranged by size:
1601 1436 1161 147 30 30 30 30
11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 174 2233 2557 2560 2806 2871 3075
Size: 112 62 2059 324 3 246 65 204
Cuts at: 3075 3252 3394 3397 4177 4476
Size: 177 142 3 780 299
Fragments arranged by size:
2059 780 324 299 246 204 177 142
112 65 62 3 3
BseYI C'CCAG_C
Cuts at: 0 17 89 1005 1107 1114 1183 1476 2781
Size: 17 72 916 102 7 69 293 1305
Cuts at: 2781 3159 3177 3705 3950 4122 4476
Size: 378 18 528 245 172 354
Fragments arranged by size:
1305 916 528 378 354 293 245 172
102 72 69 18 17 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 2278 2325 2925 3262 3535 3829 4476
Size: 524 1754 47 600 337 273 294 647
Fragments arranged by size:
1754 647 600 524 337 294 273 47
BsiHKAI G_wGCw'C
Cuts at: 0 101 134 722 1921 1949 2237 2557 2963
Size: 101 33 588 1199 28 288 320 406
Cuts at: 2963 3350 3878 4476
Size: 387 528 598
Fragments arranged by size:
1199 598 588 528 406 387 320 288
101 33 28
BsmI GAATG_Cn'
Cuts at: 0 1403 1640 3786 4476
Size: 1403 237 2146 690
Fragments arranged by size:
2146 1403 690 237
Bsp1286I G_dGCh'C
Cuts at: 0 101 134 154 503 600 608 722 813
Size: 101 33 20 349 97 8 114 91
Cuts at: 813 908 1115 1449 1470 1589 1921 1949 2196
Size: 95 207 334 21 119 332 28 247
Cuts at: 2196 2237 2557 2798 2963 3167 3184 3350 3712
Size: 41 320 241 165 204 17 166 362
Cuts at: 3712 3878 3896 3989 4185 4476
Size: 166 18 93 196 291
Fragments arranged by size:
362 349 334 332 320 291 247 241
207 204 196 166 166 165 119 114
101 97 95 93 91 41 33 28
21 20 18 17 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1242 1247 2297 3398 3680 4476
Size: 1242 5 1050 1101 282 796
Fragments arranged by size:
1242 1101 1050 796 282 5
BsrBI CCG'CTC
Cuts at: 0 48 3648 4476
Size: 48 3600 828
Fragments arranged by size:
3600 828 48
BsrDI GCAATG_nn'
Cuts at: 0 459 2918 4304 4476
Size: 459 2459 1386 172
Fragments arranged by size:
2459 1386 459 172
BsrFI r'CCGG_y
Cuts at: 0 991 1539 3712 3764 4476
Size: 991 548 2173 52 712
Fragments arranged by size:
2173 991 712 548 52
BsrGI T'GTAC_A
Cuts at: 0 2081 4476
Size: 2081 2395
BssSI C'ACGA_G
Cuts at: 0 584 779 2210 4476
Size: 584 195 1431 2266
Fragments arranged by size:
2266 1431 584 195
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4431 4476
Size: 4431 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3939 4476
Size: 3939 537
BstYI r'GATC_y
Cuts at: 0 1709 1764 2021 2726 3068 3958 4202 4476
Size: 1709 55 257 705 342 890 244 274
Fragments arranged by size:
1709 890 705 342 274 257 244 55
BstZ17I GTA'TAC
Cuts at: 0 1521 4476
Size: 1521 2955
Bsu36I CC'TnA_GG
Cuts at: 0 632 731 4476
Size: 632 99 3745
Fragments arranged by size:
3745 632 99
BtgI C'CryG_G
Cuts at: 0 503 702 963 2600 2636 3059 4100 4476
Size: 503 199 261 1637 36 423 1041 376
Fragments arranged by size:
1637 1041 503 423 376 261 199 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1920 2986 3489 4476
Size: 1920 1066 503 987
Fragments arranged by size:
1920 1066 987 503
BtsI GCAGTG_nn'
Cuts at: 0 78 1288 1595 1979 2854 2963 3451 3488
Size: 78 1210 307 384 875 109 488 37
Cuts at: 3488 3697 4078 4476
Size: 209 381 398
Fragments arranged by size:
1210 875 488 398 384 381 307 209
109 78 37
ClaI AT'CG_AT
Cuts at: 0 4214 4476
Size: 4214 262
EaeI y'GGCC_r
Cuts at: 0 43 381 1223 1334 2003 2258 2279 3140
Size: 43 338 842 111 669 255 21 861
Cuts at: 3140 3170 3320 3766 4175 4388 4476
Size: 30 150 446 409 213 88
Fragments arranged by size:
861 842 669 446 409 338 255 213
150 111 88 43 30 21
EarI CTCTTCn'nnn_
Cuts at: 0 935 1909 2671 2803 3650 3883 4476
Size: 935 974 762 132 847 233 593
Fragments arranged by size:
974 935 847 762 593 233 132
EcoICRI GAG'CTC
Cuts at: 0 720 1919 1947 2235 4476
Size: 720 1199 28 288 2241
Fragments arranged by size:
2241 1199 720 288 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 662 892 1042 1705 1861 1961 2159
Size: 416 246 230 150 663 156 100 198
Cuts at: 2159 2383 2560 2758 3836 3961 4087 4243 4476
Size: 224 177 198 1078 125 126 156 233
Fragments arranged by size:
1078 663 416 246 233 230 224 198
198 177 156 156 150 126 125 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 616 940 2572 3676 4039 4084 4476
Size: 616 324 1632 1104 363 45 392
Fragments arranged by size:
1632 1104 616 392 363 324 45
EcoO109I rG'GnC_Cy
Cuts at: 0 286 394 499 707 824 836 904 905
Size: 286 108 105 208 117 12 68 1
Cuts at: 905 1111 1137 1206 1821 3257 3528 4032 4049
Size: 206 26 69 615 1436 271 504 17
Cuts at: 4049 4062 4471 4476
Size: 13 409 5
Fragments arranged by size:
1436 615 504 409 286 271 208 206
117 108 105 69 68 26 17 13
12 5 1
FspI TGC'GCA
Cuts at: 0 4399 4476
Size: 4399 77
HaeII r_GCGC'y
Cuts at: 0 107 201 2403 2600 3249 3282 3494 4476
Size: 107 94 2202 197 649 33 212 982
Fragments arranged by size:
2202 982 649 212 197 107 94 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 1475 1507 1662 1694 2033 2065
Size: 185 32 1258 32 155 32 339 32
Cuts at: 2065 4476
Size: 2411
Fragments arranged by size:
2411 1258 339 185 155 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1308 4476
Size: 1308 3168
HindIII A'AGCT_T
Cuts at: 0 1378 2621 4476
Size: 1378 1243 1855
Fragments arranged by size:
1855 1378 1243
KasI G'GCGC_C
Cuts at: 0 197 2399 2596 3278 4476
Size: 197 2202 197 682 1198
Fragments arranged by size:
2202 1198 682 197 197
KpnI G_GTAC'C
Cuts at: 0 1303 4476
Size: 1303 3173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1809 4476
Size: 228 1581 2667
Fragments arranged by size:
2667 1581 228
MscI TGG'CCA
Cuts at: 0 383 1225 1336 2005 2281 3142 4177 4476
Size: 383 842 111 669 276 861 1035 299
Fragments arranged by size:
1035 861 842 669 383 299 276 111
MslI CAynn'nnrTG
Cuts at: 0 561 816 1364 1606 2845 3634 4082 4476
Size: 561 255 548 242 1239 789 448 394
Fragments arranged by size:
1239 789 561 548 448 394 255 242
MspA1I CmG'CkG
Cuts at: 0 21 54 93 240 325 534 1107 2079
Size: 21 33 39 147 85 209 573 972
Cuts at: 2079 2263 2736 2811 2989 3705 3939 4164 4254
Size: 184 473 75 178 716 234 225 90
Cuts at: 4254 4428 4476
Size: 174 48
Fragments arranged by size:
972 716 573 473 234 225 209 184
178 174 147 90 85 75 48 39
33 21
NarI GG'CG_CC
Cuts at: 0 198 2400 2597 3279 4476
Size: 198 2202 197 682 1197
Fragments arranged by size:
2202 1197 682 198 197
NcoI C'CATG_G
Cuts at: 0 503 4100 4476
Size: 503 3597 376
Fragments arranged by size:
3597 503 376
NheI G'CTAG_C
Cuts at: 0 987 4476
Size: 987 3489
NspI r_CATG'y
Cuts at: 0 1969 2316 4476
Size: 1969 347 2160
Fragments arranged by size:
2160 1969 347
PasI CC'CwG_GG
Cuts at: 0 496 593 3541 3687 3726 4029 4476
Size: 496 97 2948 146 39 303 447
Fragments arranged by size:
2948 496 447 303 146 97 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 2462 2649 3750 4476
Size: 348 2114 187 1101 726
Fragments arranged by size:
2114 1101 726 348 187
PfoI T'CCnGG_A
Cuts at: 0 189 935 1682 3743 4476
Size: 189 746 747 2061 733
Fragments arranged by size:
2061 747 746 733 189
PmlI CAC'GTG
Cuts at: 0 2613 3240 4476
Size: 2613 627 1236
Fragments arranged by size:
2613 1236 627
PpuMI rG'GwC_Cy
Cuts at: 0 286 394 1821 4471 4476
Size: 286 108 1427 2650 5
Fragments arranged by size:
2650 1427 286 108 5
PshAI GACnn'nnGTC
Cuts at: 0 2481 4476
Size: 2481 1995
PspOMI G'GGCC_C
Cuts at: 0 499 904 1111 4476
Size: 499 405 207 3365
Fragments arranged by size:
3365 499 405 207
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1072 1104 4476
Size: 1072 32 3372
Fragments arranged by size:
3372 1072 32
PstI C_TGCA'G
Cuts at: 0 187 533 764 1022 1776 2576 3704 4436
Size: 187 346 231 258 754 800 1128 732
Cuts at: 4436 4476
Size: 40
Fragments arranged by size:
1128 800 754 732 346 258 231 187
40
PvuII CAG'CTG
Cuts at: 0 21 93 325 534 1107 2079 2736 2811
Size: 21 72 232 209 573 972 657 75
Cuts at: 2811 2989 3705 3939 4164 4254 4428 4476
Size: 178 716 234 225 90 174 48
Fragments arranged by size:
972 716 657 573 234 232 225 209
178 174 90 75 72 48 21
SacI G_AGCT'C
Cuts at: 0 722 1921 1949 2237 4476
Size: 722 1199 28 288 2239
Fragments arranged by size:
2239 1199 722 288 28
SanDI GG'GwC_CC
Cuts at: 0 394 1821 4476
Size: 394 1427 2655
Fragments arranged by size:
2655 1427 394
SapI GCTCTTCn'nnn_
Cuts at: 0 1909 2803 3883 4476
Size: 1909 894 1080 593
Fragments arranged by size:
1909 1080 894 593
SfcI C'TryA_G
Cuts at: 0 183 529 760 1018 1354 1755 1772 1784
Size: 183 346 231 258 336 401 17 12
Cuts at: 1784 2572 3252 3369 3463 3700 4432 4476
Size: 788 680 117 94 237 732 44
Fragments arranged by size:
788 732 680 401 346 336 258 237
231 183 117 94 44 17 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 706 3361 4476
Size: 706 2655 1115
Fragments arranged by size:
2655 1115 706
SfoI GGC'GCC
Cuts at: 0 199 2401 2598 3280 4476
Size: 199 2202 197 682 1196
Fragments arranged by size:
2202 1196 682 199 197
SmaI CCC'GGG
Cuts at: 0 391 4476
Size: 391 4085
SmlI C'TyrA_G
Cuts at: 0 1469 3400 4476
Size: 1469 1931 1076
Fragments arranged by size:
1931 1469 1076
SphI G_CATG'C
Cuts at: 0 2316 4476
Size: 2316 2160
StuI AGG'CCT
Cuts at: 0 1459 1750 3155 4476
Size: 1459 291 1405 1321
Fragments arranged by size:
1459 1405 1321 291
StyI C'CwwG_G
Cuts at: 0 398 404 503 1604 1663 1700 1988 3329
Size: 398 6 99 1101 59 37 288 1341
Cuts at: 3329 4100 4476
Size: 771 376
Fragments arranged by size:
1341 1101 771 398 376 288 99 59
37 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 103 348 997 1261 1656 2023 2359 3072
Size: 103 245 649 264 395 367 336 713
Cuts at: 3072 4093 4114 4476
Size: 1021 21 362
Fragments arranged by size:
1021 713 649 395 367 362 336 264
245 103 21
TatI w'GTAC_w
Cuts at: 0 1088 2081 2508 3230 3659 4476
Size: 1088 993 427 722 429 817
Fragments arranged by size:
1088 993 817 722 429 427
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3285 3317 4476
Size: 3285 32 1159
Fragments arranged by size:
3285 1159 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 590 1329 1698 1957 4107 4230 4476
Size: 590 739 369 259 2150 123 246
Fragments arranged by size:
2150 739 590 369 259 246 123
XhoI C'TCGA_G
Cuts at: 0 1469 4476
Size: 1469 3007
XmaI C'CCGG_G
Cuts at: 0 389 4476
Size: 389 4087
XmnI GAAnn'nnTTC
Cuts at: 0 8 4476
Size: 8 4468
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI
PstI PvuII SacI SanDI SapI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII ApaLI AscI AseI AsiSI AvrII
BbvCI BcgI BfrBI BglII BsaBI BsiEI BsiWI BsmBI
BspEI BspHI BssHII BstBI BstEII CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FalI FseI FspAI
HpaI MfeI MluI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PpiI PsiI PspXI PvuI
RsrII SacII SalI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI ZraI