Restriction map for pFN21AB9403
 (Linear) (Six-base) MAPSORT of: KIBB9403.seq  Check: 9505  from: 1  to: 3816



KIBB9403.orf

 With 182 enzymes: SgfI * 

                        March 31, 2008 15:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3777    3816 
   Size:       3777      39
 
AatII G_ACGT'C

Cuts at:      0    2078    3816 
   Size:       2078    1738
 
Acc65I G'GTAC_C

Cuts at:      0    1232    3032    3608    3816 
   Size:       1232    1800     576     208

  Fragments arranged by size: 

                1800    1232     576     208
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     768     886    1534    1996    2062    2356    2383    2469 
   Size:        768     118     648     462      66     294      27      86

Cuts at:   2469    2698    3816 
   Size:        229    1118

  Fragments arranged by size: 

                1118     768     648     462     294     229     118      86
                  66      27
 
AfeI AGC'GCT

Cuts at:      0     719    1929    3553    3816 
   Size:        719    1210    1624     263

  Fragments arranged by size: 

                1624    1210     719     263
 
AleI CACnn'nnGTG

Cuts at:      0    2857    3816 
   Size:       2857     959
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     646    1684    3816 
   Size:        646    1038    2132

  Fragments arranged by size: 

                2132    1038     646
 
AlwNI CAG_nnn'CTG

Cuts at:      0     849    2373    3042    3393    3808    3816 
   Size:        849    1524     669     351     415       8

  Fragments arranged by size: 

                1524     849     669     415     351       8
 
ApaI G_GGCC'C

Cuts at:      0    1323    3022    3816 
   Size:       1323    1699     794

  Fragments arranged by size: 

                1699    1323     794
 
ApaLI G'TGCA_C

Cuts at:      0     700    3816 
   Size:        700    3116
 
ApoI r'AATT_y

Cuts at:      0     286    2489    3816 
   Size:        286    2203    1327

  Fragments arranged by size: 

                2203    1327     286
 
AvaI C'yCGr_G

Cuts at:      0     141     616    1440    3816 
   Size:        141     475     824    2376

  Fragments arranged by size: 

                2376     824     475     141
 
AvrII C'CTAG_G

Cuts at:      0    3006    3816 
   Size:       3006     810
 
BanI G'GyrC_C

Cuts at:      0     202    1232    1552    1677    2529    3032    3608    3816 
   Size:        202    1030     320     125     852     503     576     208

  Fragments arranged by size: 

                1030     852     576     503     320     208     202     125
            
 
BanII G_rGCy'C

Cuts at:      0     134     748    1323    1450    2142    2291    3022    3059 
   Size:        134     614     575     127     692     149     731      37

Cuts at:   3059    3816 
   Size:        757

  Fragments arranged by size: 

                 757     731     692     614     575     149     134     127
                  37
 
BbeI G_GCGC'C

Cuts at:      0     206    3816 
   Size:        206    3610
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66    1336    1734    2132    3816 
   Size:         66    1270     398     398    1684

  Fragments arranged by size: 

                1684    1270     398     398      66
 
BbvCI CC'TCA_GC

Cuts at:      0    2692    2980    3328    3816 
   Size:       2692     288     348     488

  Fragments arranged by size: 

                2692     488     348     288
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     127    1093    1127    3621    3655    3816 
   Size:         93      34     966      34    2494      34     161

  Fragments arranged by size: 

                2494     966     161      93      34      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0      65    1145    3766    3816 
   Size:         65    1080    2621      50

  Fragments arranged by size: 

                2621    1080      65      50
 
BglII A'GATC_T

Cuts at:      0    2430    3348    3816 
   Size:       2430     918     468

  Fragments arranged by size: 

                2430     918     468
 
BlpI GC'TnA_GC

Cuts at:      0    2285    3816 
   Size:       2285    1531
 
Bme1580I G_kGCm'C

Cuts at:      0     704    1323    1682    1848    3022    3816 
   Size:        704     619     359     166    1174     794

  Fragments arranged by size: 

                1174     794     704     619     359     166
 
BmgBI CAC'GTC

Cuts at:      0    3071    3816 
   Size:       3071     745
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1320    1928    2591    3295    3816 
   Size:       1320     608     663     704     521

  Fragments arranged by size: 

                1320     704     663     608     521
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2305    3816 
   Size:       2305    1511
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     415    1075    1954    2008    2098    2197    2277 
   Size:        257     158     660     879      54      90      99      80

Cuts at:   2277    2650    3064    3253    3365    3502    3816 
   Size:        373     414     189     112     137     314

  Fragments arranged by size: 

                 879     660     414     373     314     257     189     158
                 137     112      99      90      80      54
 
Bpu10I CC'TnA_GC

Cuts at:      0      35    2692    2966    2980    3328    3548    3816 
   Size:         35    2657     274      14     348     220     268

  Fragments arranged by size: 

                2657     348     274     268     220      35      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2011    2854    3473    3610    3816 
   Size:       2011     843     619     137     206

  Fragments arranged by size: 

                2011     843     619     206     137
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     452    1091    2204    2891    3130    3816 
   Size:        452     639    1113     687     239     686

  Fragments arranged by size: 

                1113     687     686     639     452     239
 
BsaAI yAC'GTr

Cuts at:      0     642    2664    3558    3816 
   Size:        642    2022     894     258

  Fragments arranged by size: 

                2022     894     642     258
 
BsaHI Gr'CG_yC

Cuts at:      0     203    2075    3816 
   Size:        203    1872    1741

  Fragments arranged by size: 

                1872    1741     203
 
BsaWI w'CCGG_w

Cuts at:      0    1070    1794    2514    3116    3816 
   Size:       1070     724     720     602     700

  Fragments arranged by size: 

                1070     724     720     700     602
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1873    1903    2932    2962    3084    3114    3169    3199 
   Size:       1873      30    1029      30     122      30      55      30

Cuts at:   3199    3816 
   Size:        617

  Fragments arranged by size: 

                1873    1029     617     122      55      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     196     211    1120    1440    1818    1821    3238    3445 
   Size:        196      15     909     320     378       3    1417     207

Cuts at:   3445    3607    3816 
   Size:        162     209

  Fragments arranged by size: 

                1417     909     378     320     209     207     196     162
                  15       3
 
BseYI C'CCAG_C

Cuts at:      0     176     376     634    1824    2219    2648    2687    2907 
   Size:        176     200     258    1190     395     429      39     220

Cuts at:   2907    2996    3583    3773    3816 
   Size:         89     587     190      43

  Fragments arranged by size: 

                1190     587     429     395     258     220     200     190
                 176      89      43      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     496     824    3239    3472    3816 
   Size:         84     412     328    2415     233     344

  Fragments arranged by size: 

                2415     412     344     328     233      84
 
BsiEI CG_ry'CG

Cuts at:      0      17     859    2950    3337    3816 
   Size:         17     842    2091     387     479

  Fragments arranged by size: 

                2091     842     479     387      17
 
BsiHKAI G_wGCw'C

Cuts at:      0     704    1310    1429    2291    3816 
   Size:        704     606     119     862    1525

  Fragments arranged by size: 

                1525     862     704     606     119
 
BsmI GAATG_Cn'

Cuts at:      0     241    1190    3816 
   Size:        241     949    2626

  Fragments arranged by size: 

                2626     949     241
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     704     748    1310    1323    1429    1450    1682 
   Size:        134     570      44     562      13     106      21     232

Cuts at:   1682    1848    2142    2291    3022    3059    3816 
   Size:        166     294     149     731      37     757

  Fragments arranged by size: 

                 757     731     570     562     294     232     166     149
                 134     106      44      37      21      13
 
BspEI T'CCGG_A

Cuts at:      0    2514    3116    3816 
   Size:       2514     602     700

  Fragments arranged by size: 

                2514     700     602
 
BspHI T'CATG_A

Cuts at:      0     930    3816 
   Size:        930    2886
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     173    1478    3020    3392    3777    3816 
   Size:        173    1305    1542     372     385      39

  Fragments arranged by size: 

                1542    1305     385     372     173      39
 
BsrBI CCG'CTC

Cuts at:      0     158     891    1685    3339    3816 
   Size:        158     733     794    1654     477

  Fragments arranged by size: 

                1654     794     733     477     158
 
BsrDI GCAATG_nn'

Cuts at:      0    3095    3816 
   Size:       3095     721
 
BsrFI r'CCGG_y

Cuts at:      0    3332    3816 
   Size:       3332     484
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2253    2373    3459    3816 
   Size:       2253     120    1086     357

  Fragments arranged by size: 

                2253    1086     357     120
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     448    1378    2310    3816 
   Size:        448     930     932    1506

  Fragments arranged by size: 

                1506     932     930     448
 
BstYI r'GATC_y

Cuts at:      0    1998    2430    3348    3567    3626    3816 
   Size:       1998     432     918     219      59     190

  Fragments arranged by size: 

                1998     918     432     219     190      59
 
Bsu36I CC'TnA_GG

Cuts at:      0     662    3675    3816 
   Size:        662    3013     141

  Fragments arranged by size: 

                3013     662     141
 
BtgI C'CryG_G

Cuts at:      0     171     371     491    1901    3816 
   Size:        171     200     120    1410    1915

  Fragments arranged by size: 

                1915    1410     200     171     120
 
BtsI GCAGTG_nn'

Cuts at:      0    2453    2920    3809    3816 
   Size:       2453     467     889       7

  Fragments arranged by size: 

                2453     889     467       7
 
DraIII CAC_nnn'GTG

Cuts at:      0    1559    3816 
   Size:       1559    2257
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2074    3816 
   Size:       2074    1742
 
EaeI y'GGCC_r

Cuts at:      0      66     939     981    1380    1756    3334    3816 
   Size:         66     873      42     399     376    1578     482

  Fragments arranged by size: 

                1578     873     482     399     376      66      42
 
EagI C'GGCC_G

Cuts at:      0    3334    3816 
   Size:       3334     482
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     448     862    1421    1523    2385    2647    3569 
   Size:        296     152     414     559     102     862     262     922

Cuts at:   3569    3816 
   Size:        247

  Fragments arranged by size: 

                 922     862     559     414     296     262     247     152
                 102
 
EcoICRI GAG'CTC

Cuts at:      0    2289    3816 
   Size:       2289    1527
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     415     768     886    1075    1534    1954    1996 
   Size:        257     158     353     118     189     459     420      42

Cuts at:   1996    2008    2062    2098    2197    2277    2356    2383    2469 
   Size:         12      54      36      99      80      79      27      86

Cuts at:   2469    2650    2698    3064    3253    3365    3502    3816 
   Size:        181      48     366     189     112     137     314

  Fragments arranged by size: 

                 459     420     366     353     314     257     189     189
                 181     158     137     118     112      99      86      80
                  79      54      48      42      36      27      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1724    2026    3816 
   Size:       1724     302    1790

  Fragments arranged by size: 

                1790    1724     302
 
EcoO109I rG'GnC_Cy

Cuts at:      0     773     949    1047    1319    1375    1729    2019    2109 
   Size:        773     176      98     272      56     354     290      90

Cuts at:   2109    2265    2768    2865    3019    3258    3816 
   Size:        156     503      97     154     239     558

  Fragments arranged by size: 

                 773     558     503     354     290     272     239     176
                 156     154      98      97      90      56
 
EcoRI G'AATT_C

Cuts at:      0     286    2489    3816 
   Size:        286    2203    1327

  Fragments arranged by size: 

                2203    1327     286
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    3168    3189    3816 
   Size:       3168      21     627

  Fragments arranged by size: 

                3168     627      21
 
FspI TGC'GCA

Cuts at:      0    1493    3816 
   Size:       1493    2323
 
HaeII r_GCGC'y

Cuts at:      0     206     721    1536    1587    1618    1931    3183    3555 
   Size:        206     515     815      51      31     313    1252     372

Cuts at:   3555    3816 
   Size:        261

  Fragments arranged by size: 

                1252     815     515     372     313     261     206      51
                  31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     401     433     707     739     954     986 
   Size:         28      32     341      32     274      32     215      32

Cuts at:    986    1350    1382    1873    1905    2932    2964    3340    3372 
   Size:        364      32     491      32    1027      32     376      32

Cuts at:   3372    3523    3555    3816 
   Size:        151      32     261

  Fragments arranged by size: 

                1027     491     376     364     341     274     261     215
                 151      32      32      32      32      32      32      32
                  32      32      28
 
HincII GTy'rAC

Cuts at:      0     138     999    3816 
   Size:        138     861    2817

  Fragments arranged by size: 

                2817     861     138
 
KasI G'GCGC_C

Cuts at:      0     202    3816 
   Size:        202    3614
 
KpnI G_GTAC'C

Cuts at:      0    1236    3036    3612    3816 
   Size:       1236    1800     576     204

  Fragments arranged by size: 

                1800    1236     576     204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2321    3455    3816 
   Size:       2321    1134     361

  Fragments arranged by size: 

                2321    1134     361
 
MscI TGG'CCA

Cuts at:      0     983    1382    3816 
   Size:        983     399    2434

  Fragments arranged by size: 

                2434     983     399
 
MslI CAynn'nnrTG

Cuts at:      0     709     935    1547    1693    2308    2857    3816 
   Size:        709     226     612     146     615     549     959

  Fragments arranged by size: 

                 959     709     615     612     549     226     146
 
MspA1I CmG'CkG

Cuts at:      0      98     173     742    1239    1974    2361    2652    2691 
   Size:         98      75     569     497     735     387     291      39

Cuts at:   2691    2984    3231    3717    3816 
   Size:        293     247     486      99

  Fragments arranged by size: 

                 735     569     497     486     387     293     291     247
                  99      98      75      39
 
NaeI GCC'GGC

Cuts at:      0    3334    3816 
   Size:       3334     482
 
NarI GG'CG_CC

Cuts at:      0     203    3816 
   Size:        203    3613
 
NcoI C'CATG_G

Cuts at:      0    1901    3816 
   Size:       1901    1915
 
NdeI CA'TA_TG

Cuts at:      0     317    1200    3816 
   Size:        317     883    2616

  Fragments arranged by size: 

                2616     883     317
 
NgoMIV G'CCGG_C

Cuts at:      0    3332    3816 
   Size:       3332     484
 
NspI r_CATG'y

Cuts at:      0    1671    3657    3816 
   Size:       1671    1986     159

  Fragments arranged by size: 

                1986    1671     159
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2121    2226    3816 
   Size:       2121     105    1590

  Fragments arranged by size: 

                2121    1590     105
 
PfoI T'CCnGG_A

Cuts at:      0    2114    2405    3816 
   Size:       2114     291    1411

  Fragments arranged by size: 

                2114    1411     291
 
PpuMI rG'GwC_Cy

Cuts at:      0     773     949    1047    1375    1729    2019    2109    2265 
   Size:        773     176      98     328     354     290      90     156

Cuts at:   2265    3816 
   Size:       1551

  Fragments arranged by size: 

                1551     773     354     328     290     176     156      98
                  90
 
PspOMI G'GGCC_C

Cuts at:      0    1319    3018    3816 
   Size:       1319    1699     798

  Fragments arranged by size: 

                1699    1319     798
 
PstI C_TGCA'G

Cuts at:      0    2261    2378    2460    3389    3602    3722    3816 
   Size:       2261     117      82     929     213     120      94

  Fragments arranged by size: 

                2261     929     213     120     117      94      82
 
PvuI CG_AT'CG

Cuts at:      0     859    3816 
   Size:        859    2957
 
PvuII CAG'CTG

Cuts at:      0    1974    2361    2652    2691    3231    3717    3816 
   Size:       1974     387     291      39     540     486      99

  Fragments arranged by size: 

                1974     540     486     387     291      99      39
 
SacI G_AGCT'C

Cuts at:      0    2291    3816 
   Size:       2291    1525
 
SacII CC_GC'GG

Cuts at:      0     174    3816 
   Size:        174    3642
 
SanDI GG'GwC_CC

Cuts at:      0     949    1047    3816 
   Size:        949      98    2769

  Fragments arranged by size: 

                2769     949      98
 
SapI GCTCTTCn'nnn_

Cuts at:      0     296    1523    2385    3569    3816 
   Size:        296    1227     862    1184     247

  Fragments arranged by size: 

                1227    1184     862     296     247
 
SbfI CC_TGCA'GG

Cuts at:      0    2378    3816 
   Size:       2378    1438
 
SfcI C'TryA_G

Cuts at:      0    2257    2374    2456    2787    3385    3598    3664    3718 
   Size:       2257     117      82     331     598     213      66      54

Cuts at:   3718    3816 
   Size:         98

  Fragments arranged by size: 

                2257     598     331     213     117      98      82      66
                  54
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1145    3766    3816 
   Size:       1145    2621      50

  Fragments arranged by size: 

                2621    1145      50
 
SfoI GGC'GCC

Cuts at:      0     204    3816 
   Size:        204    3612
 
SmlI C'TyrA_G

Cuts at:      0    2026    2869    3488    3589    3816 
   Size:       2026     843     619     101     227

  Fragments arranged by size: 

                2026     843     619     227     101
 
SphI G_CATG'C

Cuts at:      0    1671    3816 
   Size:       1671    2145
 
SspI AAT'ATT

Cuts at:      0     685    3816 
   Size:        685    3131
 
StuI AGG'CCT

Cuts at:      0    2462    3769    3816 
   Size:       2462    1307      47

  Fragments arranged by size: 

                2462    1307      47
 
StyI C'CwwG_G

Cuts at:      0    1349    1626    1735    1837    1901    3006    3173    3291 
   Size:       1349     277     109     102      64    1105     167     118

Cuts at:   3291    3816 
   Size:        525

  Fragments arranged by size: 

                1349    1105     525     277     167     118     109     102
                  64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     811    1514    1898    2696    3597    3816 
   Size:        811     703     384     798     901     219

  Fragments arranged by size: 

                 901     811     798     703     384     219
 
TatI w'GTAC_w

Cuts at:      0    3185    3816 
   Size:       3185     631
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     907     939    3084    3116    3816 
   Size:        907      32    2145      32     700

  Fragments arranged by size: 

                2145     907     700      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     994    2107    2269    3816 
   Size:        994    1113     162    1547

  Fragments arranged by size: 

                1547    1113     994     162
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     489    1899    3816 
   Size:        489    1410    1917

  Fragments arranged by size: 

                1917    1410     489
 
XmnI GAAnn'nnTTC

Cuts at:      0     290    3816 
   Size:        290    3526
 
ZraI GAC'GTC

Cuts at:      0    2076    3816 
   Size:       2076    1740
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     BtsI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
    PflMI     PfoI    PpuMI   PspOMI     PstI     PvuI    PvuII     SacI
    SacII    SanDI     SapI     SbfI     SfcI     SfiI     SfoI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII   AflIII     AgeI     AhdI     AloI     AscI
     AseI    AsiSI     BaeI    BamHI    BciVI     BclI    BfrBI     BmtI
    BsaBI    BsiWI    BsmBI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI     DraI     EciI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI    RsrII     SalI     ScaI    SexAI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI     XbaI     XhoI
     XmaI
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