Restriction map for
pFN21ASDB3800
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII AccIII AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AvaI AviII
BbeI BbrPI BbvCI BcnI BfrI
BglI BsaHI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI BstBI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI FauI FseI FspI
Hin1I KasI KspI MluI MroI
MspA1I NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 3045 GTATAC GT^[AC][GT]AC
AccII 3462 CGCG CG^CG
AclI 3514 AACGTT AA^CGTT
AflIII 3786 ACGTGT A^C[GA][CT]GT
ApaI 3921 GGGCCC GGGCC^C
AspI 2278 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 592 CCTAGG C^CTAGG
BalI 3834 TGGCCA TGG^CCA
BamHI 333 GGATCC G^GATCC
BbsI 28 GAAGAC GAAGAC(2/6)
BciVI 3334 GGATAC GTATCC(6/5)
BlnI 592 CCTAGG C^CTAGG
BlpI 2152 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2152 GCTCAGC GC^T[ACGT]AGC
BpuAI 28 GAAGAC GAAGAC(2/6)
BsaAI 3785 TACGTG [CT]AC^GT[GA]
BsaI 455 GAGACC GGTCTC(1/5)
BseRI 667 GAGGAG GAGGAG(10/8)
BsgI 2692 GTGCAG GTGCAG(16/14)
Bsp1407I 3219 TGTACA T^GTACA
BspHI 3011 TCATGA T^CATGA
BspMI 1027 GCAGGT ACCTGC(4/8)
BsrFI 3885 GCCGGT [GA]^CCGG[CT]
BsrGI 3219 TGTACA T^GTACA
Bst1107I 3045 GTATAC GTA^TAC
BstEII 1309 GGTGACC G^GT[ACGT]ACC
BstPI 1309 GGTGACC G^GT[ACGT]ACC
BstUI 3462 CGCG CG^CG
BstZ17I 3045 GTATAC GTA^TAC
CelII 2152 GCTCAGC GC^T[ACGT]AGC
Cfr10I 3885 GCCGGT [GA]^CCGG[CT]
DraIII 2686 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3834 TGGCCA [CT]^GGCC[GA]
EcoO65I 1309 GGTGACC G^GT[ACGT]ACC
EcoRV 2674 GATATC GAT^ATC
HgaI 3460 GACGC GACGC(5/10)
HindIII 3716 AAGCTT A^AGCTT
HpaI 370 GTTAAC GTT^AAC
MfeI 3663 CAATTG C^AATTG
MluNI 3834 TGGCCA TGG^CCA
MscI 3834 TGGCCA TGG^CCA
MunI 3663 CAATTG C^AATTG
MvnI 3462 CGCG CG^CG
PacI 37 TTAATTAA TTAAT^TAA
PflFI 2278 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 566 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3514 AACGTT AA^CGTT
RcaI 3011 TCATGA T^CATGA
ScaI 2832 AGTACT AGT^ACT
SphI 2604 GCATGC GCATG^C
SspBI 3219 TGTACA T^GTACA
SspI 1246 AATATT AAT^ATT
StuI 2072 AGGCCT AGG^CCT
Tth111I 2278 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1032 TCTAGA T^CTAGA
XcmI 510 CCAGGGAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 1782 GGTACC G^GTACC
2112 GGTACC
AciI 3457 GCGG CCGC(-3/-1)
3463 GCGG
AocI 976 CCTGAGG CC^T[ACGT]AGG
2075 CCTCAGG
Asp718I 1782 GGTACC G^GTACC
2112 GGTACC
AvaII 567 GGACC G^G[AT]CC
3375 GGTCC
BcgI 2694 GCAGCTTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3521 GCAAGGGTGTCG
BclI 63 TGATCA T^GATCA
270 TGATCA
BsaBI 1702 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATTCTTATC
BsmBI 2317 GAGACG CGTCTC(1/5)
2995 GAGACG
BsmFI 2739 GGGAC GGGAC(10/14)
3780 GGGAC
BsrDI 2377 GCAATG GCAATG(2/0)
3830 GCAATG
BstAPI 3172 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3411 GCAGGCACTGC
BstXI 2102 CCACTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3350 CCAGTATTGTGG
Bsu36I 976 CCTGAGG CC^T[ACGT]AGG
2075 CCTCAGG
CfoI 3314 GCGC GCG^C
3875 GCGC
EarI 3327 CTCTTC CTCTTC(1/4)
2452 GAAGAG
Eco81I 976 CCTGAGG CC^T[ACGT]AGG
2075 CCTCAGG
EcoRI 315 GAATTC G^AATTC
847 GAATTC
EcoT22I 154 ATGCAT ATGCA^T
2602 ATGCAT
FbaI 63 TGATCA T^GATCA
270 TGATCA
HaeII 3313 GGCGCT [GA]GCGC^[CT]
3874 AGCGCC
HhaI 3314 GCGC GCG^C
3875 GCGC
HinP1I 3314 GCGC G^CGC
3875 GCGC
HincII 370 GTTAAC GT[CT]^[GA]AC
903 GTTGAC
HindII 370 GTTAAC GT[CT]^[GA]AC
903 GTTGAC
KpnI 1782 GGTACC GGTAC^C
2112 GGTACC
Ksp632I 3327 CTCTTC CTCTTC(1/4)
2452 GAAGAG
MaeII 3515 ACGT A^CGT
3786 ACGT
MamI 1702 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2862 GATTCTTATC
NsiI 154 ATGCAT ATGCA^T
2602 ATGCAT
PflMI 2983 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3492 CCAGTGGGTGG
SacI 2081 GAGCTC GAGCT^C
2088 GAGCTC
TaqI 1619 TCGA T^CGA
2940 TCGA
TthHB8I 1619 TCGA T^CGA
2940 TCGA
Van91I 2983 CCATTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3492 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI BanI BanII
BbvI BfaI BglII BmyI BpmI
BsaJI BsaWI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI