(Circular) (Six-base) MAPSORT of: pFN21ASDB1384.seq Check: 8454 from: 1 to: 6751 pFN21ASDB1384 6751 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3431 3431 Size: 6751 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4561 278 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3828 4094 1937 Size: 1891 266 4594 Fragments arranged by size: 4594 1891 266 Acc65I G'GTAC_C Cuts at: 4072 4072 Size: 6751 AclI AA'CG_TT Cuts at: 1563 4878 5251 1563 Size: 3315 373 3063 Fragments arranged by size: 3315 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3276 4814 6198 6466 1632 Size: 1644 1538 1384 268 1917 Fragments arranged by size: 1917 1644 1538 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6554 AflIII A'CryG_T Cuts at: 1203 5650 6627 1203 Size: 4447 977 1327 Fragments arranged by size: 4447 1327 977 AgeI A'CCGG_T Cuts at: 5582 5582 Size: 6751 AhdI GACnn_n'nnGTC Cuts at: 5480 5480 Size: 6751 AleI CACnn'nnGTG Cuts at: 1166 2033 2670 1166 Size: 867 637 5247 Fragments arranged by size: 5247 867 637 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4014 1184 Size: 2830 3921 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2669 2701 2669 Size: 32 6719 AlwNI CAG_nnn'CTG Cuts at: 2194 3025 3160 3488 4494 6066 2194 Size: 831 135 328 1006 1572 2879 Fragments arranged by size: 2879 1572 1006 831 328 135 ApaLI G'TGCA_C Cuts at: 4808 5964 4808 Size: 1156 5595 ApoI r'AATT_y Cuts at: 1493 2698 3235 3405 3506 3613 4060 4286 4438 Size: 1205 537 170 101 107 447 226 152 Cuts at: 4438 6547 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1205 537 447 226 170 152 107 101 AseI AT'TA_AT Cuts at: 160 1032 5305 160 Size: 872 4273 1606 Fragments arranged by size: 4273 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 AvaI C'yCGr_G Cuts at: 1870 1943 4076 1870 Size: 73 2133 4545 Fragments arranged by size: 4545 2133 73 BamHI G'GATC_C Cuts at: 4081 4448 4081 Size: 367 6384 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4072 5527 618 Size: 325 217 627 2285 1455 1842 Fragments arranged by size: 2285 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3359 4070 1391 Size: 1968 711 4072 Fragments arranged by size: 4072 1968 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3064 3195 928 Size: 971 1165 131 4484 Fragments arranged by size: 4484 1165 971 131 BbvCI CC'TCA_GC Cuts at: 2358 3077 3447 3543 2358 Size: 719 370 96 5566 Fragments arranged by size: 5566 719 370 96 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4942 4976 1903 Size: 34 3005 34 3678 Fragments arranged by size: 3678 3005 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2880 4643 5853 2880 Size: 1763 1210 3778 Fragments arranged by size: 3778 1763 1210 BclI T'GATC_A Cuts at: 1548 3490 1548 Size: 1942 4809 BfrBI ATG'CAT Cuts at: 3703 3703 Size: 6751 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2912 5362 136 Size: 107 122 71 631 605 1240 2450 1525 Fragments arranged by size: 2450 1525 1240 631 605 122 107 71 BglII A'GATC_T Cuts at: 2642 6746 2642 Size: 4104 2647 BlpI GC'TnA_GC Cuts at: 4168 4168 Size: 6751 Bme1580I G_kGCm'C Cuts at: 1790 4812 5968 1790 Size: 3022 1156 2573 Fragments arranged by size: 3022 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6751 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3209 3561 4482 Size: 416 53 83 399 24 1792 352 921 Cuts at: 4482 5440 442 Size: 958 1753 Fragments arranged by size: 1792 1753 958 921 416 399 352 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2110 2308 2559 3225 5411 1239 Size: 871 198 251 666 2186 2579 Fragments arranged by size: 2579 2186 871 666 251 198 Bpu10I CC'TnA_GC Cuts at: 2358 3077 3447 3543 2358 Size: 719 370 96 5566 Fragments arranged by size: 5566 719 370 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4876 5741 6039 4876 Size: 865 298 5588 Fragments arranged by size: 5588 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5414 882 Size: 617 3915 2219 Fragments arranged by size: 3915 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4048 4542 493 Size: 711 2844 494 2702 Fragments arranged by size: 2844 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6745 6745 Size: 6751 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4558 4940 5575 275 Size: 53 83 186 936 3025 382 635 1451 Fragments arranged by size: 3025 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5184 5582 5856 6003 6414 5184 Size: 398 274 147 411 5521 Fragments arranged by size: 5521 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6526 Fragments arranged by size: 6526 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6751 BseYI C'CCAG_C Cuts at: 5954 6450 5954 Size: 496 6255 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2122 2446 1163 Size: 573 386 324 5468 Fragments arranged by size: 5468 573 386 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 4201 4962 5990 1540 Size: 275 186 33 2167 761 1028 2301 Fragments arranged by size: 2301 2167 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 4812 4897 5968 4812 Size: 85 1071 5595 Fragments arranged by size: 5595 1071 85 BsmI GAATG_Cn' Cuts at: 3920 3988 4262 4355 3920 Size: 68 274 93 6316 Fragments arranged by size: 6316 274 93 68 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3359 4070 4812 4897 5968 1391 Size: 399 1569 711 742 85 1071 2174 Fragments arranged by size: 2174 1569 1071 742 711 399 85 BspEI T'CCGG_A Cuts at: 6414 6414 Size: 6751 BspHI T'CATG_A Cuts at: 4640 6370 4640 Size: 1730 5021 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2046 2510 2770 3431 4106 844 Size: 1202 464 260 661 675 3489 Fragments arranged by size: 3489 1202 675 661 464 260 BsrBI CCG'CTC Cuts at: 1394 1823 4638 1394 Size: 429 2815 3507 Fragments arranged by size: 3507 2815 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2397 5246 5420 65 Size: 1183 1149 2849 174 1396 Fragments arranged by size: 2849 1396 1183 1149 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2040 2051 5395 5582 1700 Size: 110 230 11 3344 187 2869 Fragments arranged by size: 3344 2869 230 187 110 11 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6751 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6751 BssSI C'ACGA_G Cuts at: 4811 5823 4811 Size: 1012 5739 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 5177 BstBI TT'CG_AA Cuts at: 2007 5555 2007 Size: 3548 3203 BstEII G'GTnAC_C Cuts at: 1184 3432 1184 Size: 2248 4503 BstXI CCAn_nnnn'nTGG Cuts at: 3089 3474 3089 Size: 385 6366 BstYI r'GATC_y Cuts at: 1972 2642 4081 4448 4833 4850 6302 6388 6400 Size: 670 1439 367 385 17 1452 86 12 Cuts at: 6400 6746 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1439 670 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2028 513 Size: 552 957 6 5236 Fragments arranged by size: 5236 957 552 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6012 Fragments arranged by size: 6012 683 56 BtsI GCAGTG_nn' Cuts at: 2427 2751 3812 4267 5061 5081 2427 Size: 324 1061 455 794 20 4097 Fragments arranged by size: 4097 1061 794 455 324 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6751 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6716 DraI TTT'AAA Cuts at: 3141 4055 3141 Size: 914 5837 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5758 809 Size: 507 4442 1802 Fragments arranged by size: 4442 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2031 4198 Size: 54 1425 50 275 18 195 6 2167 Cuts at: 4198 5087 6716 8 Size: 889 1629 43 Fragments arranged by size: 2167 1629 1425 889 275 195 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2031 4198 1537 Size: 275 219 2167 4090 Fragments arranged by size: 4090 2167 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2897 4681 1353 Size: 1544 1784 3423 Fragments arranged by size: 3423 1784 1544 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5338 5712 5858 387 Size: 1064 138 3749 374 146 1280 Fragments arranged by size: 3749 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2308 2559 3225 3276 4814 5411 Size: 393 478 198 251 666 51 1538 597 Cuts at: 5411 6198 6466 1239 Size: 787 268 1524 Fragments arranged by size: 1538 1524 787 666 597 478 393 268 251 198 51 EcoO109I rG'GnC_Cy Cuts at: 3022 3366 3022 Size: 344 6407 EcoRI G'AATT_C Cuts at: 4060 4438 4060 Size: 378 6373 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3634 3634 Size: 6751 FspI TGC'GCA Cuts at: 2496 5257 2496 Size: 2761 3990 HaeII r_GCGC'y Cuts at: 1678 5898 1678 Size: 4220 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2370 2634 2666 3154 Size: 32 135 32 766 32 264 32 488 Cuts at: 3154 3186 5392 5424 5466 5498 1373 Size: 32 2206 32 42 32 2626 Fragments arranged by size: 2626 2206 766 488 264 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2581 3810 3829 4095 4341 669 Size: 988 281 643 1229 19 266 246 3079 Fragments arranged by size: 3079 1229 988 643 281 266 246 19 HindIII A'AGCT_T Cuts at: 748 2739 3842 748 Size: 1991 1103 3657 Fragments arranged by size: 3657 1991 1103 HpaI GTT'AAC Cuts at: 2581 4341 2581 Size: 1760 4991 KpnI G_GTAC'C Cuts at: 4076 4076 Size: 6751 MfeI C'AATT_G Cuts at: 3103 4350 3103 Size: 1247 5504 MluI A'CGCG_T Cuts at: 6627 6627 Size: 6751 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5865 6049 1305 Size: 4560 184 2007 Fragments arranged by size: 4560 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 10 Size: 54 1425 343 195 4734 Fragments arranged by size: 4734 1425 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 2670 2756 2772 3981 4709 5068 Size: 648 867 637 86 16 1209 728 359 Cuts at: 5068 5227 518 Size: 159 2042 Fragments arranged by size: 2042 1209 867 728 648 637 359 159 86 16 MspA1I CmG'CkG Cuts at: 1606 4167 4844 5992 6237 1606 Size: 2561 677 1148 245 2120 Fragments arranged by size: 2561 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2042 2053 2042 Size: 11 6740 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5242 Fragments arranged by size: 5242 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6751 NgoMIV G'CCGG_C Cuts at: 2040 2051 2040 Size: 11 6740 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6751 NotI GC'GGCC_GC Cuts at: 4198 4198 Size: 6751 NsiI A_TGCA'T Cuts at: 3705 3705 Size: 6751 NspI r_CATG'y Cuts at: 1132 1229 4109 5654 1132 Size: 97 2880 1545 2229 Fragments arranged by size: 2880 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2431 1348 Size: 1083 5668 PciI A'CATG_T Cuts at: 5650 5650 Size: 6751 PflMI CCAn_nnn'nTGG Cuts at: 1108 2742 1108 Size: 1634 5117 PmeI GTTT'AAAC Cuts at: 4055 4055 Size: 6751 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4820 4852 6333 6365 4820 Size: 32 1481 32 5206 Fragments arranged by size: 5206 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3022 3366 3022 Size: 344 6407 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6751 PsiI TTA'TAA Cuts at: 2153 4321 2153 Size: 2168 4583 PstI C_TGCA'G Cuts at: 830 2422 4003 4103 830 Size: 1592 1581 100 3478 Fragments arranged by size: 3478 1592 1581 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6751 SalI G'TCGA_C Cuts at: 1936 3827 4093 1936 Size: 1891 266 4594 Fragments arranged by size: 4594 1891 266 SanDI GG'GwC_CC Cuts at: 3366 3366 Size: 6751 SbfI CC_TGCA'GG Cuts at: 4103 4103 Size: 6751 ScaI AGT'ACT Cuts at: 3836 4999 6711 3836 Size: 1163 1712 3876 Fragments arranged by size: 3876 1712 1163 SexAI A'CCwGG_T Cuts at: 3085 3085 Size: 6751 SfcI C'TryA_G Cuts at: 826 1045 2418 2554 2560 3999 4099 5234 5915 Size: 219 1373 136 6 1439 100 1135 681 Cuts at: 5915 6106 826 Size: 191 1471 Fragments arranged by size: 1471 1439 1373 1135 681 219 191 136 100 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6751 SmaI CCC'GGG Cuts at: 1872 4078 1872 Size: 2206 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4855 5756 6018 820 Size: 197 926 2912 901 262 1553 Fragments arranged by size: 2912 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4048 4542 493 Size: 3555 494 2702 Fragments arranged by size: 3555 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6751 SphI G_CATG'C Cuts at: 1132 4109 1132 Size: 2977 3774 SspI AAT'ATT Cuts at: 5 52 3044 4675 5 Size: 47 2992 1631 2081 Fragments arranged by size: 2992 2081 1631 47 StuI AGG'CCT Cuts at: 1517 3668 1517 Size: 2151 4600 StyI C'CwwG_G Cuts at: 513 1065 2022 4190 513 Size: 552 957 2168 3074 Fragments arranged by size: 3074 2168 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3675 4778 4795 4948 5133 5578 325 Size: 1255 2095 1103 17 153 185 445 1498 Fragments arranged by size: 2095 1498 1255 1103 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2439 3834 4997 Size: 274 80 33 51 1443 462 1395 1163 Cuts at: 4997 6709 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1395 1163 462 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 3873 3905 6711 6743 2057 Size: 32 1784 32 2806 32 2065 Fragments arranged by size: 2806 2065 1784 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6751 XbaI T'CTAG_A Cuts at: 4087 4087 Size: 6751 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6751 XmaI C'CCGG_G Cuts at: 1870 4076 1870 Size: 2206 4545 XmnI GAAnn'nnTTC Cuts at: 4880 4880 Size: 6751 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4559 276 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI