(Circular) (Six-base) MAPSORT of: pFN21ASDB0238.seq Check: 4951 from: 1 to: 8500 pFN21ASDB0238 8500 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3903 4989 3903 Size: 1086 7414 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6310 278 Size: 53 83 186 936 4774 2468 Fragments arranged by size: 4774 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5843 1937 Size: 3906 4594 Acc65I G'GTAC_C Cuts at: 4986 5821 4986 Size: 835 7665 AclI AA'CG_TT Cuts at: 1563 6627 7000 1563 Size: 5064 373 3063 Fragments arranged by size: 5064 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3413 3578 4738 4985 5140 6563 7947 8215 Size: 1781 165 1160 247 155 1423 1384 268 Cuts at: 8215 1632 Size: 1917 Fragments arranged by size: 1917 1781 1423 1384 1160 268 247 165 155 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8303 AflIII A'CryG_T Cuts at: 1203 2043 4353 7399 8376 1203 Size: 840 2310 3046 977 1327 Fragments arranged by size: 3046 2310 1327 977 840 AgeI A'CCGG_T Cuts at: 7331 7331 Size: 8500 AhdI GACnn_n'nnGTC Cuts at: 7229 7229 Size: 8500 AleI CACnn'nnGTG Cuts at: 1166 3221 4364 5501 1166 Size: 2055 1143 1137 4165 Fragments arranged by size: 4165 2055 1143 1137 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2567 1184 Size: 1383 7117 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3429 3461 5291 5323 5660 5678 5692 5710 3429 Size: 32 1830 32 337 18 14 18 6219 Fragments arranged by size: 6219 1830 337 32 32 18 18 14 AlwNI CAG_nnn'CTG Cuts at: 2070 2920 3403 6243 7815 2070 Size: 850 483 2840 1572 2755 Fragments arranged by size: 2840 2755 1572 850 483 ApaI G_GGCC'C Cuts at: 3594 4308 4737 5024 3594 Size: 714 429 287 7070 Fragments arranged by size: 7070 714 429 287 ApaLI G'TGCA_C Cuts at: 6557 7713 6557 Size: 1156 7344 ApoI r'AATT_y Cuts at: 1493 3974 5809 6035 6187 8296 1493 Size: 2481 1835 226 152 2109 1697 Fragments arranged by size: 2481 2109 1835 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7054 160 Size: 872 6022 1606 Fragments arranged by size: 6022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 AvaI C'yCGr_G Cuts at: 1870 1943 2090 4112 4784 5825 1870 Size: 73 147 2022 672 1041 4545 Fragments arranged by size: 4545 2022 1041 672 147 73 BamHI G'GATC_C Cuts at: 3132 5830 6197 3132 Size: 2698 367 5435 Fragments arranged by size: 5435 2698 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2151 2384 2423 2666 3182 Size: 325 217 627 364 233 39 243 516 Cuts at: 3182 3744 3865 3994 4284 4639 4986 5313 5821 Size: 562 121 129 290 355 347 327 508 Cuts at: 5821 7276 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 562 516 508 364 355 347 327 325 290 243 233 217 129 121 39 BanII G_rGCy'C Cuts at: 1391 3258 3594 3857 4122 4308 4422 4737 5024 Size: 1867 336 263 265 186 114 315 287 Cuts at: 5024 5243 5819 1391 Size: 219 576 4072 Fragments arranged by size: 4072 1867 576 336 315 287 265 263 219 186 114 BbeI G_GCGC'C Cuts at: 2155 3186 3748 3998 4288 2155 Size: 1031 562 250 290 6367 Fragments arranged by size: 6367 1031 562 290 250 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2155 3945 5336 928 Size: 971 256 1790 1391 4092 Fragments arranged by size: 4092 1790 1391 971 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3355 3389 3586 3620 6691 6725 1903 Size: 34 1418 34 197 34 3071 34 3678 Fragments arranged by size: 3678 3071 1418 197 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 6392 7602 4556 Size: 1836 1210 5454 Fragments arranged by size: 5454 1836 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2592 2862 4857 Size: 107 122 71 631 605 920 270 1995 Cuts at: 4857 7111 136 Size: 2254 1525 Fragments arranged by size: 2254 1995 1525 920 631 605 270 122 107 71 BglII A'GATC_T Cuts at: 2547 8495 2547 Size: 5948 2552 BlpI GC'TnA_GC Cuts at: 5917 5917 Size: 8500 Bme1580I G_kGCm'C Cuts at: 1790 2387 2428 2986 3390 3594 3835 4308 4371 Size: 597 41 558 404 204 241 473 63 Cuts at: 4371 4644 4737 5024 6561 7717 1790 Size: 273 93 287 1537 1156 2573 Fragments arranged by size: 2573 1537 1156 597 558 473 404 287 273 241 204 93 63 41 BmgBI CAC'GTC Cuts at: 1318 4356 1318 Size: 3038 5462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 2984 3204 Size: 416 53 83 399 24 1436 131 220 Cuts at: 3204 3384 6231 7189 442 Size: 180 2847 958 1753 Fragments arranged by size: 2847 1753 1436 958 416 399 220 180 131 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5376 5376 Size: 8500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2749 2828 3139 3467 3686 3946 4464 4600 Size: 1510 79 311 328 219 260 518 136 Cuts at: 4600 4721 4820 5579 7160 1239 Size: 121 99 759 1581 2579 Fragments arranged by size: 2579 1581 1510 759 518 328 311 260 219 136 121 99 79 Bpu10I CC'TnA_GC Cuts at: 3194 4470 5752 3194 Size: 1276 1282 5942 Fragments arranged by size: 5942 1282 1276 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 4037 4483 6625 7490 7788 2171 Size: 1866 446 2142 865 298 2883 Fragments arranged by size: 2883 2142 1866 865 446 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3471 3690 4135 4196 5528 7163 882 Size: 617 1972 219 445 61 1332 1635 2219 Fragments arranged by size: 2219 1972 1635 1332 617 445 219 61 BsaAI yAC'GTr Cuts at: 493 1204 2044 3636 4224 4876 5797 6291 493 Size: 711 840 1592 588 652 921 494 2702 Fragments arranged by size: 2702 1592 921 840 711 652 588 494 BsaBI GATnn'nnATC Cuts at: 8494 8494 Size: 8500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2152 3183 3745 3995 Size: 53 83 186 936 619 1031 562 250 Cuts at: 3995 4285 6307 6689 7324 275 Size: 290 2022 382 635 1451 Fragments arranged by size: 2022 1451 1031 936 635 619 562 382 290 250 186 83 53 BsaWI w'CCGG_w Cuts at: 2523 3168 3497 4236 6933 7331 7605 7752 8163 Size: 645 329 739 2697 398 274 147 411 Cuts at: 8163 2523 Size: 2860 Fragments arranged by size: 2860 2697 739 645 411 398 329 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2285 2315 3369 3399 4793 Size: 30 165 30 882 30 1054 30 1394 Cuts at: 4793 4823 4865 4895 5132 5162 5225 5255 5291 Size: 30 42 30 237 30 63 30 36 Cuts at: 5291 5321 1178 Size: 30 4357 Fragments arranged by size: 4357 1394 1054 882 237 165 63 42 36 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2048 2948 3158 5414 5588 1186 Size: 859 3 900 210 2256 174 4098 Fragments arranged by size: 4098 2256 900 859 210 174 3 BseYI C'CCAG_C Cuts at: 2266 2380 3668 4121 7703 8199 2266 Size: 114 1288 453 3582 496 2567 Fragments arranged by size: 3582 2567 1288 496 453 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2532 3014 3224 3875 4298 4727 Size: 573 501 295 482 210 651 423 429 Cuts at: 4727 1163 Size: 4936 Fragments arranged by size: 4936 651 573 501 482 429 423 295 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2881 3694 4526 5522 5950 6711 Size: 275 186 880 813 832 996 428 761 Cuts at: 6711 7739 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 996 880 832 813 761 428 275 186 BsiHKAI G_wGCw'C Cuts at: 2619 4866 4881 5243 6561 6646 7717 2619 Size: 2247 15 362 1318 85 1071 3402 Fragments arranged by size: 3402 2247 1318 1071 362 85 15 BsiWI C'GTAC_G Cuts at: 5038 5038 Size: 8500 BsmI GAATG_Cn' Cuts at: 3471 6011 6104 3471 Size: 2540 93 5867 Fragments arranged by size: 5867 2540 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5128 5128 Size: 8500 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2387 2428 2619 2986 3258 3390 3594 Size: 399 597 41 191 367 272 132 204 Cuts at: 3594 3835 3857 4122 4308 4371 4422 4644 4737 Size: 241 22 265 186 63 51 222 93 Cuts at: 4737 4866 4881 5024 5243 5819 6561 6646 7717 Size: 129 15 143 219 576 742 85 1071 Cuts at: 7717 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 597 576 399 367 272 265 241 222 219 204 191 186 143 132 129 93 85 63 51 41 22 15 BspEI T'CCGG_A Cuts at: 2523 8163 2523 Size: 5640 2860 BspHI T'CATG_A Cuts at: 6389 8119 6389 Size: 1730 6770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3897 3903 4989 5268 5855 844 Size: 3053 6 1086 279 587 3489 Fragments arranged by size: 3489 3053 1086 587 279 6 BsrBI CCG'CTC Cuts at: 1394 1823 2572 4234 6387 1394 Size: 429 749 1662 2153 3507 Fragments arranged by size: 3507 2153 1662 749 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5749 6995 7169 65 Size: 1183 4501 1246 174 1396 Fragments arranged by size: 4501 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3246 3862 4525 4868 7144 7331 1700 Size: 110 1436 616 663 343 2276 187 2869 Fragments arranged by size: 2869 2276 1436 663 616 343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8500 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8500 BssSI C'ACGA_G Cuts at: 4299 6560 7572 4299 Size: 2261 1012 5227 Fragments arranged by size: 5227 2261 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2070 794 Size: 1276 7224 BstBI TT'CG_AA Cuts at: 2007 7304 2007 Size: 5297 3203 BstEII G'GTnAC_C Cuts at: 1184 3112 3223 3625 1184 Size: 1928 111 402 6059 Fragments arranged by size: 6059 1928 402 111 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3053 3710 4213 5711 2029 Size: 1024 657 503 1498 4818 Fragments arranged by size: 4818 1498 1024 657 503 BstYI r'GATC_y Cuts at: 1972 2085 2403 2526 2547 3132 3303 5070 5283 Size: 113 318 123 21 585 171 1767 213 Cuts at: 5283 5830 6197 6582 6599 8051 8137 8149 8495 Size: 547 367 385 17 1452 86 12 346 Cuts at: 8495 1972 Size: 1977 Fragments arranged by size: 1977 1767 1452 585 547 385 367 346 318 213 171 123 113 86 21 17 12 Bsu36I CC'TnA_GG Cuts at: 4152 4290 4152 Size: 138 8362 BtgI C'CryG_G Cuts at: 513 1065 2022 2147 2457 3006 3869 4558 5781 Size: 552 957 125 310 549 863 689 1223 Cuts at: 5781 513 Size: 3232 Fragments arranged by size: 3232 1223 957 863 689 552 549 310 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2800 5586 487 Size: 683 56 1574 2786 3401 Fragments arranged by size: 3401 2786 1574 683 56 BtsI GCAGTG_nn' Cuts at: 2986 3348 4286 5258 5638 6016 6810 6830 2986 Size: 362 938 972 380 378 794 20 4656 Fragments arranged by size: 4656 972 938 794 380 378 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8465 DraI TTT'AAA Cuts at: 5804 5804 Size: 8500 DraIII CAC_nnn'GTG Cuts at: 2113 2779 5253 2113 Size: 666 2474 5360 Fragments arranged by size: 5360 2474 666 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2705 3086 7507 809 Size: 507 1389 381 4421 1802 Fragments arranged by size: 4421 1802 1389 507 381 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2415 2435 2961 Size: 54 1425 50 275 18 585 20 526 Cuts at: 2961 3150 3264 3798 4523 4777 5646 5701 5947 Size: 189 114 534 725 254 869 55 246 Cuts at: 5947 6836 8465 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 869 725 585 534 526 275 254 246 189 114 55 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 4523 5947 1537 Size: 275 2711 1424 4090 Fragments arranged by size: 4090 2711 1424 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2256 2756 5184 6430 1353 Size: 903 500 2428 1246 3423 Fragments arranged by size: 3423 2428 1246 903 500 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7087 7461 7607 387 Size: 1064 138 5498 374 146 1280 Fragments arranged by size: 5498 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5241 5241 Size: 8500 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2749 2828 3139 3413 3467 3578 3686 Size: 393 1117 79 311 274 54 111 108 Cuts at: 3686 3946 4464 4600 4721 4738 4820 4985 5140 Size: 260 518 136 121 17 82 165 155 Cuts at: 5140 5579 6563 7160 7947 8215 1239 Size: 439 984 597 787 268 1524 Fragments arranged by size: 1524 1117 984 787 597 518 439 393 311 274 268 260 165 155 136 121 111 108 82 79 54 17 EcoNI CCTnn'n_nnAGG Cuts at: 4538 4898 4538 Size: 360 8140 EcoO109I rG'GnC_Cy Cuts at: 3099 3462 3590 3591 4304 4305 4733 4936 5020 Size: 363 128 1 713 1 428 203 84 Cuts at: 5020 5021 5131 5257 5553 3099 Size: 1 110 126 296 6046 Fragments arranged by size: 6046 713 428 363 296 203 128 126 110 84 1 1 1 EcoRI G'AATT_C Cuts at: 5809 6187 5809 Size: 378 8122 FspI TGC'GCA Cuts at: 7006 7006 Size: 8500 HaeII r_GCGC'y Cuts at: 1678 2036 2155 3186 3383 3748 3998 4288 7647 Size: 358 119 1031 197 365 250 290 3359 Cuts at: 7647 1678 Size: 2531 Fragments arranged by size: 3359 2531 1031 365 358 290 250 197 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 4793 4825 5537 Size: 32 135 32 1046 32 2143 32 712 Cuts at: 5537 5569 7141 7173 7215 7247 1373 Size: 32 1572 32 42 32 2626 Fragments arranged by size: 2626 2143 1572 1046 712 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4396 5844 6090 669 Size: 988 281 2458 1448 246 3079 Fragments arranged by size: 3079 2458 1448 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8500 HpaI GTT'AAC Cuts at: 6090 6090 Size: 8500 KasI G'GCGC_C Cuts at: 2151 3182 3744 3994 4284 2151 Size: 1031 562 250 290 6367 Fragments arranged by size: 6367 1031 562 290 250 KpnI G_GTAC'C Cuts at: 4990 5825 4990 Size: 835 7665 MfeI C'AATT_G Cuts at: 6099 6099 Size: 8500 MluI A'CGCG_T Cuts at: 8376 8376 Size: 8500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 3909 4374 7614 7798 1305 Size: 1344 1260 465 3240 184 2007 Fragments arranged by size: 3240 2007 1344 1260 465 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2437 3152 3266 3800 4779 Size: 54 1425 343 605 715 114 534 979 Cuts at: 4779 5703 10 Size: 924 2807 Fragments arranged by size: 2807 1425 979 924 715 605 534 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2390 3051 3221 4211 4364 5501 Size: 648 861 363 661 170 990 153 1137 Cuts at: 5501 6458 6817 6976 518 Size: 957 359 159 2042 Fragments arranged by size: 2042 1137 990 957 861 661 648 363 359 170 159 153 MspA1I CmG'CkG Cuts at: 1606 2226 2361 2917 2955 3292 3792 4560 5102 Size: 620 135 556 38 337 500 768 542 Cuts at: 5102 5916 6593 7741 7986 1606 Size: 814 677 1148 245 2120 Fragments arranged by size: 2120 1148 814 768 677 620 556 542 500 337 245 135 38 NarI GG'CG_CC Cuts at: 2152 3183 3745 3995 4285 2152 Size: 1031 562 250 290 6367 Fragments arranged by size: 6367 1031 562 290 250 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6991 Fragments arranged by size: 6991 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8500 NotI GC'GGCC_GC Cuts at: 5947 5947 Size: 8500 NspI r_CATG'y Cuts at: 1132 1229 2993 5858 7403 1132 Size: 97 1764 2865 1545 2229 Fragments arranged by size: 2865 2229 1764 1545 97 PasI CC'CwG_GG Cuts at: 1348 3466 3857 5626 1348 Size: 2118 391 1769 4222 Fragments arranged by size: 4222 2118 1769 391 PciI A'CATG_T Cuts at: 7399 7399 Size: 8500 PflMI CCAn_nnn'nTGG Cuts at: 1108 2116 2779 2847 3763 4636 4894 1108 Size: 1008 663 68 916 873 258 4714 Fragments arranged by size: 4714 1008 916 873 663 258 68 PfoI T'CCnGG_A Cuts at: 2805 3702 3923 2805 Size: 897 221 7382 Fragments arranged by size: 7382 897 221 PmeI GTTT'AAAC Cuts at: 5804 5804 Size: 8500 PmlI CAC'GTG Cuts at: 3636 4224 3636 Size: 588 7912 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5291 5323 5372 5404 6569 6601 8082 Size: 32 1234 32 49 32 1165 32 1481 Cuts at: 8082 8114 4025 Size: 32 4411 Fragments arranged by size: 4411 1481 1234 1165 49 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3462 4936 5131 5257 5553 3462 Size: 1474 195 126 296 6409 Fragments arranged by size: 6409 1474 296 195 126 PshAI GACnn'nnGTC Cuts at: 1613 4142 1613 Size: 2529 5971 PsiI TTA'TAA Cuts at: 6070 6070 Size: 8500 PspOMI G'GGCC_C Cuts at: 3590 4304 4733 5020 3590 Size: 714 429 287 7070 Fragments arranged by size: 7070 714 429 287 PstI C_TGCA'G Cuts at: 830 2066 2916 3937 5265 5852 830 Size: 1236 850 1021 1328 587 3478 Fragments arranged by size: 3478 1328 1236 1021 850 587 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8500 PvuII CAG'CTG Cuts at: 2917 3292 3792 2917 Size: 375 500 7625 Fragments arranged by size: 7625 500 375 SacI G_AGCT'C Cuts at: 5243 5243 Size: 8500 SacII CC_GC'GG Cuts at: 4561 4561 Size: 8500 SalI G'TCGA_C Cuts at: 1936 5842 1936 Size: 3906 4594 SanDI GG'GwC_CC Cuts at: 3462 4936 5131 3462 Size: 1474 195 6831 Fragments arranged by size: 6831 1474 195 SapI GCTCTTCn'nnn_ Cuts at: 2256 2756 2256 Size: 500 8000 SbfI CC_TGCA'GG Cuts at: 5852 5852 Size: 8500 ScaI AGT'ACT Cuts at: 3728 6748 8460 3728 Size: 3020 1712 3768 Fragments arranged by size: 3768 3020 1712 SexAI A'CCwGG_T Cuts at: 2775 2775 Size: 8500 SfcI C'TryA_G Cuts at: 826 1045 2062 2912 3769 3787 3933 4684 5113 Size: 219 1017 850 857 18 146 751 429 Cuts at: 5113 5261 5848 6983 7664 7855 826 Size: 148 587 1135 681 191 1471 Fragments arranged by size: 1471 1135 1017 857 850 751 681 587 429 219 191 148 146 18 SfoI GGC'GCC Cuts at: 2153 3184 3746 3996 4286 2153 Size: 1031 562 250 290 6367 Fragments arranged by size: 6367 1031 562 290 250 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8500 SmaI CCC'GGG Cuts at: 1872 4114 5827 1872 Size: 2242 1713 4545 Fragments arranged by size: 4545 2242 1713 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4052 4498 6604 7505 7767 Size: 197 926 243 1866 446 2106 901 262 Cuts at: 7767 820 Size: 1553 Fragments arranged by size: 2106 1866 1553 926 901 446 262 243 197 SnaBI TAC'GTA Cuts at: 493 5797 6291 493 Size: 5304 494 2702 Fragments arranged by size: 5304 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8500 SphI G_CATG'C Cuts at: 1132 2993 5858 1132 Size: 1861 2865 3774 Fragments arranged by size: 3774 2865 1861 SspI AAT'ATT Cuts at: 5 52 6424 5 Size: 47 6372 2081 Fragments arranged by size: 6372 2081 47 StuI AGG'CCT Cuts at: 1517 3371 3644 4712 5582 1517 Size: 1854 273 1068 870 4435 Fragments arranged by size: 4435 1854 1068 870 273 StyI C'CwwG_G Cuts at: 513 1065 2022 2256 5136 5181 5939 513 Size: 552 957 234 2880 45 758 3074 Fragments arranged by size: 3074 2880 957 758 552 234 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 4979 5513 5536 6527 6544 6697 Size: 1255 678 2721 534 23 991 17 153 Cuts at: 6697 6882 7327 325 Size: 185 445 1498 Fragments arranged by size: 2721 1498 1255 991 678 534 445 185 153 23 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3726 5547 5706 Size: 274 80 33 51 1443 1749 1821 159 Cuts at: 5706 6746 8458 96 Size: 1040 1712 138 Fragments arranged by size: 1821 1749 1712 1443 1040 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2097 2129 2285 2317 3163 3195 3369 3401 4554 Size: 32 156 32 846 32 174 32 1153 Cuts at: 4554 4586 5132 5164 8460 8492 2097 Size: 32 546 32 3296 32 2105 Fragments arranged by size: 3296 2105 1153 846 546 174 156 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2882 1316 Size: 1566 6934 XbaI T'CTAG_A Cuts at: 5836 5836 Size: 8500 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2263 2377 2773 3114 3217 3463 3808 2263 Size: 114 396 341 103 246 345 6955 Fragments arranged by size: 6955 396 345 341 246 114 103 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8500 XmaI C'CCGG_G Cuts at: 1870 4112 5825 1870 Size: 2242 1713 4545 Fragments arranged by size: 4545 2242 1713 XmnI GAAnn'nnTTC Cuts at: 6629 6629 Size: 8500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6308 276 Size: 53 83 186 936 4774 2468 Fragments arranged by size: 4774 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SfiI SrfI SwaI