Restriction map for
pFN21ASDB0185
(Linear) (Six-base) MAPSORT of: KIBB0185.seq Check: 5685 from: 1 to: 4938
KIBB0185.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1167 4787 4916 4938
Size: 1167 3620 129 22
Fragments arranged by size:
3620 1167 129 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1062 1086 1744 1772 1930 2584 2838
Size: 889 173 24 658 28 158 654 254
Cuts at: 2838 2895 3796 4938
Size: 57 901 1142
Fragments arranged by size:
1142 901 889 658 654 254 173 158
57 28 24
AfeI AGC'GCT
Cuts at: 0 2193 4938
Size: 2193 2745
AflIII A'CryG_T
Cuts at: 0 488 3566 4938
Size: 488 3078 1372
Fragments arranged by size:
3078 1372 488
AhdI GACnn_n'nnGTC
Cuts at: 0 4446 4938
Size: 4446 492
AleI CACnn'nnGTG
Cuts at: 0 914 1529 1972 4938
Size: 914 615 443 2966
Fragments arranged by size:
2966 914 615 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 922 932 1990 4235 4938
Size: 922 10 1058 2245 703
Fragments arranged by size:
2245 1058 922 703 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3553 3585 4874 4906 4938
Size: 3553 32 1289 32 32
Fragments arranged by size:
3553 1289 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 192 711 902 1182 1770 2426 2616 2769
Size: 192 519 191 280 588 656 190 153
Cuts at: 2769 2980 3248 4938
Size: 211 268 1690
Fragments arranged by size:
1690 656 588 519 280 268 211 192
191 190 153
ApaLI G'TGCA_C
Cuts at: 0 4566 4938
Size: 4566 372
ApoI r'AATT_y
Cuts at: 0 1613 4938
Size: 1613 3325
AscI GG'CGCG_CC
Cuts at: 0 2415 4938
Size: 2415 2523
AvaI C'yCGr_G
Cuts at: 0 171 1354 1902 2743 3726 4505 4938
Size: 171 1183 548 841 983 779 433
Fragments arranged by size:
1183 983 841 779 548 433 171
BamHI G'GATC_C
Cuts at: 0 110 4938
Size: 110 4828
BanI G'GyrC_C
Cuts at: 0 1531 2089 2619 2891 4005 4475 4938
Size: 1531 558 530 272 1114 470 463
Fragments arranged by size:
1531 1114 558 530 470 463 272
BanII G_rGCy'C
Cuts at: 0 434 896 1898 2484 2528 2675 2816 3983
Size: 434 462 1002 586 44 147 141 1167
Cuts at: 3983 4506 4938
Size: 523 432
Fragments arranged by size:
1167 1002 586 523 462 434 432 147
141 44
BbeI G_GCGC'C
Cuts at: 0 4479 4938
Size: 4479 459
BbsI GAAGACnn'nnnn_
Cuts at: 0 393 1236 1349 1546 2144 2322 4096 4881
Size: 393 843 113 197 598 178 1774 785
Cuts at: 4881 4938
Size: 57
Fragments arranged by size:
1774 843 785 598 393 197 178 113
57
BbvCI CC'TCA_GC
Cuts at: 0 1268 2692 2801 4795 4938
Size: 1268 1424 109 1994 143
Fragments arranged by size:
1994 1424 1268 143 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2706 2740 3316 3350 4386 4420 4938
Size: 2706 34 576 34 1036 34 518
Fragments arranged by size:
2706 1036 576 518 34 34 34
BclI T'GATC_A
Cuts at: 0 305 4938
Size: 305 4633
BglI GCCn_nnn'nGGC
Cuts at: 0 2032 2650 3079 3202 3754 4070 4938
Size: 2032 618 429 123 552 316 868
Fragments arranged by size:
2032 868 618 552 429 316 123
BglII A'GATC_T
Cuts at: 0 4871 4938
Size: 4871 67
BlpI GC'TnA_GC
Cuts at: 0 805 895 4393 4938
Size: 805 90 3498 545
Fragments arranged by size:
3498 805 545 90
Bme1580I G_kGCm'C
Cuts at: 0 155 300 1660 1750 2171 2732 4010 4570
Size: 155 145 1360 90 421 561 1278 560
Cuts at: 4570 4938
Size: 368
Fragments arranged by size:
1360 1278 561 560 421 368 155 145
90
BmgBI CAC'GTC
Cuts at: 0 1738 3330 4938
Size: 1738 1592 1608
Fragments arranged by size:
1738 1608 1592
BmrI ACTGGGnnnn_n'
Cuts at: 0 2436 2531 3287 4938
Size: 2436 95 756 1651
Fragments arranged by size:
2436 1651 756 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1917 4938
Size: 1917 3021
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1807 1905 2203 2284 2857 2957 3738
Size: 933 874 98 298 81 573 100 781
Cuts at: 3738 4357 4717 4843 4938
Size: 619 360 126 95
Fragments arranged by size:
933 874 781 619 573 360 298 126
100 98 95 81
Bpu10I CC'TnA_GC
Cuts at: 0 10 1268 1819 2692 2801 4286 4795 4894
Size: 10 1258 551 873 109 1485 509 99
Cuts at: 4894 4938
Size: 44
Fragments arranged by size:
1485 1258 873 551 509 109 99 44
10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2059 4612 4938
Size: 2059 2553 326
Fragments arranged by size:
2553 2059 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 1124 1469 1573 2009 2254 3051 3793 4938
Size: 1124 345 104 436 245 797 742 1145
Fragments arranged by size:
1145 1124 797 742 436 345 245 104
BsaAI yAC'GTr
Cuts at: 0 2166 3237 3567 4938
Size: 2166 1071 330 1371
Fragments arranged by size:
2166 1371 1071 330
BsaBI GATnn'nnATC
Cuts at: 0 115 1417 4938
Size: 115 1302 3521
Fragments arranged by size:
3521 1302 115
BsaHI Gr'CG_yC
Cuts at: 0 1913 4476 4938
Size: 1913 2563 462
Fragments arranged by size:
2563 1913 462
BsaWI w'CCGG_w
Cuts at: 0 1637 3842 4938
Size: 1637 2205 1096
Fragments arranged by size:
2205 1637 1096
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 198 228 1780 1810 1903 1933 2035 2065
Size: 198 30 1552 30 93 30 102 30
Cuts at: 2065 3221 3251 3262 3292 3634 3664 4015 4045
Size: 1156 30 11 30 342 30 351 30
Cuts at: 4045 4843 4873 4938
Size: 798 30 65
Fragments arranged by size:
1552 1156 798 351 342 198 102 93
65 30 30 30 30 30 30 30
30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 19 937 2506 2509 2655 2854 3642
Size: 16 3 918 1569 3 146 199 788
Cuts at: 3642 3861 4135 4564 4714 4938
Size: 219 274 429 150 224
Fragments arranged by size:
1569 918 788 429 274 224 219 199
150 146 16 3 3
BseYI C'CCAG_C
Cuts at: 0 1182 1518 1718 2010 2147 2194 2283 2753
Size: 1182 336 200 292 137 47 89 470
Cuts at: 2753 2757 3059 3392 3525 3937 4068 4619 4799
Size: 4 302 333 133 412 131 551 180
Cuts at: 4799 4938
Size: 139
Fragments arranged by size:
1182 551 470 412 336 333 302 292
200 180 139 137 133 131 89 47
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 868 892 1756 2518 3220 3235 3630
Size: 661 207 24 864 762 702 15 395
Cuts at: 3630 4063 4609 4690 4938
Size: 433 546 81 248
Fragments arranged by size:
864 762 702 661 546 433 395 248
207 81 24 15
BsiEI CG_ry'CG
Cuts at: 0 1469 2936 3738 4938
Size: 1469 1467 802 1200
Fragments arranged by size:
1469 1467 1200 802
BsiHKAI G_wGCw'C
Cuts at: 0 11 410 1040 1211 1703 2233 2675 2828
Size: 11 399 630 171 492 530 442 153
Cuts at: 2828 3983 4399 4570 4757 4938
Size: 1155 416 171 187 181
Fragments arranged by size:
1155 630 530 492 442 416 399 187
181 171 171 153 11
BsmI GAATG_Cn'
Cuts at: 0 3151 4938
Size: 3151 1787
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3625 4938
Size: 3625 1313
Bsp1286I G_dGCh'C
Cuts at: 0 11 155 300 410 434 896 1040 1211
Size: 11 144 145 110 24 462 144 171
Cuts at: 1211 1660 1703 1750 1898 2171 2233 2484 2528
Size: 449 43 47 148 273 62 251 44
Cuts at: 2528 2675 2732 2816 2828 3983 4010 4399 4506
Size: 147 57 84 12 1155 27 389 107
Cuts at: 4506 4570 4757 4938
Size: 64 187 181
Fragments arranged by size:
1155 462 449 389 273 251 187 181
171 148 147 145 144 144 110 107
84 64 62 57 47 44 43 27
24 12 11
BspEI T'CCGG_A
Cuts at: 0 1637 3842 4938
Size: 1637 2205 1096
Fragments arranged by size:
2205 1637 1096
BspHI T'CATG_A
Cuts at: 0 965 4938
Size: 965 3973
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1167 2015 2638 3143 3815 4787 4916 4938
Size: 1167 848 623 505 672 972 129 22
Fragments arranged by size:
1167 972 848 672 623 505 129 22
BsrBI CCG'CTC
Cuts at: 0 1823 2889 4938
Size: 1823 1066 2049
Fragments arranged by size:
2049 1823 1066
BsrDI GCAATG_nn'
Cuts at: 0 94 830 2288 3122 4938
Size: 94 736 1458 834 1816
Fragments arranged by size:
1816 1458 834 736 94
BsrFI r'CCGG_y
Cuts at: 0 2092 3737 4399 4938
Size: 2092 1645 662 539
Fragments arranged by size:
2092 1645 662 539
BssHII G'CGCG_C
Cuts at: 0 2415 4938
Size: 2415 2523
BssSI C'ACGA_G
Cuts at: 0 766 2407 3456 4499 4938
Size: 766 1641 1049 1043 439
Fragments arranged by size:
1641 1049 1043 766 439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1770 3833 4005 4938
Size: 1770 2063 172 933
Fragments arranged by size:
2063 1770 933 172
BstEII G'GTnAC_C
Cuts at: 0 717 1221 2830 3507 4938
Size: 717 504 1609 677 1431
Fragments arranged by size:
1609 1431 717 677 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 387 2985 3558 4356 4458 4938
Size: 387 2598 573 798 102 480
Fragments arranged by size:
2598 798 573 480 387 102
BstYI r'GATC_y
Cuts at: 0 110 1634 1938 4871 4938
Size: 110 1524 304 2933 67
Fragments arranged by size:
2933 1524 304 110 67
Bsu36I CC'TnA_GG
Cuts at: 0 3762 3893 4938
Size: 3762 131 1045
Fragments arranged by size:
3762 1045 131
BtgI C'CryG_G
Cuts at: 0 395 2318 2456 3146 3752 4019 4466 4493
Size: 395 1923 138 690 606 267 447 27
Cuts at: 4493 4938
Size: 445
Fragments arranged by size:
1923 690 606 447 445 395 267 138
27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 936 1839 1848 2007 3474 4389 4938
Size: 735 201 903 9 159 1467 915 549
Fragments arranged by size:
1467 915 903 735 549 201 159 9
BtsI GCAGTG_nn'
Cuts at: 0 2056 2124 2535 2791 2978 3446 4938
Size: 2056 68 411 256 187 468 1492
Fragments arranged by size:
2056 1492 468 411 256 187 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2430 2465 4938
Size: 2430 35 2473
Fragments arranged by size:
2473 2430 35
DraIII CAC_nnn'GTG
Cuts at: 0 2166 4938
Size: 2166 2772
EaeI y'GGCC_r
Cuts at: 0 389 635 770 1466 2033 2651 2987 3158
Size: 389 246 135 696 567 618 336 171
Cuts at: 3158 3239 3351 3443 3735 3755 4016 4577 4938
Size: 81 112 92 292 20 261 561 361
Fragments arranged by size:
696 618 567 561 389 361 336 292
261 246 171 135 112 92 81 20
EagI C'GGCC_G
Cuts at: 0 1466 3735 4938
Size: 1466 2269 1203
Fragments arranged by size:
2269 1466 1203
EarI CTCTTCn'nnn_
Cuts at: 0 91 946 982 1636 2680 3145 3793 4938
Size: 91 855 36 654 1044 465 648 1145
Fragments arranged by size:
1145 1044 855 654 648 465 91 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 206 617 3867 4938
Size: 206 411 3250 1071
Fragments arranged by size:
3250 1071 411 206
EcoICRI GAG'CTC
Cuts at: 0 2673 3981 4938
Size: 2673 1308 957
Fragments arranged by size:
2673 1308 957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 933 1062 1086 1744 1772 1807 1905
Size: 889 44 129 24 658 28 35 98
Cuts at: 1905 1930 2203 2284 2584 2838 2857 2895 2957
Size: 25 273 81 300 254 19 38 62
Cuts at: 2957 3738 3796 4357 4717 4843 4938
Size: 781 58 561 360 126 95
Fragments arranged by size:
889 781 658 561 360 300 273 254
129 126 98 95 81 62 58 44
38 35 28 25 24 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 218 1432 1495 2488 2566 3456 4938
Size: 218 1214 63 993 78 890 1482
Fragments arranged by size:
1482 1214 993 890 218 78 63
EcoO109I rG'GnC_Cy
Cuts at: 0 63 1398 1728 2060 2613 2747 3270 3920
Size: 63 1335 330 332 553 134 523 650
Cuts at: 3920 4301 4326 4560 4938
Size: 381 25 234 378
Fragments arranged by size:
1335 650 553 523 381 378 332 330
234 134 63 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2350 4938
Size: 2350 2588
FspI TGC'GCA
Cuts at: 0 292 883 3520 4938
Size: 292 591 2637 1418
Fragments arranged by size:
2637 1418 591 292
FspAI rTGC'GCAy
Cuts at: 0 883 3520 4938
Size: 883 2637 1418
Fragments arranged by size:
2637 1418 883
HaeII r_GCGC'y
Cuts at: 0 25 2195 3074 3725 4293 4479 4696 4938
Size: 25 2170 879 651 568 186 217 242
Fragments arranged by size:
2170 879 651 568 242 217 186 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 358 390 1903 1935 2006 2038 2471 2503
Size: 358 32 1513 32 71 32 433 32
Cuts at: 2503 3262 3294 4015 4047 4432 4464 4843 4875
Size: 759 32 721 32 385 32 379 32
Cuts at: 4875 4938
Size: 63
Fragments arranged by size:
1513 759 721 433 385 379 358 71
63 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1149 2940 4938
Size: 1149 1791 1998
Fragments arranged by size:
1998 1791 1149
KasI G'GCGC_C
Cuts at: 0 4475 4938
Size: 4475 463
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 2492 3074 4158 4938
Size: 122 2370 582 1084 780
Fragments arranged by size:
2370 1084 780 582 122
MscI TGG'CCA
Cuts at: 0 772 2035 2653 3160 3241 4579 4938
Size: 772 1263 618 507 81 1338 359
Fragments arranged by size:
1338 1263 772 618 507 359 81
MslI CAynn'nnrTG
Cuts at: 0 316 680 883 914 1529 1972 2317 3514
Size: 316 364 203 31 615 443 345 1197
Cuts at: 3514 3877 4456 4585 4938
Size: 363 579 129 353
Fragments arranged by size:
1197 615 579 443 364 363 353 345
316 203 129 31
MspA1I CmG'CkG
Cuts at: 0 18 441 699 786 902 1604 1722 1950
Size: 18 423 258 87 116 702 118 228
Cuts at: 1950 2151 2506 2582 2757 2769 3529 4002 4194
Size: 201 355 76 175 12 760 473 192
Cuts at: 4194 4646 4799 4938
Size: 452 153 139
Fragments arranged by size:
760 702 473 452 423 355 258 228
201 192 175 153 139 118 116 87
76 18 12
NaeI GCC'GGC
Cuts at: 0 2094 4938
Size: 2094 2844
NarI GG'CG_CC
Cuts at: 0 4476 4938
Size: 4476 462
NcoI C'CATG_G
Cuts at: 0 2318 2456 3146 3752 4938
Size: 2318 138 690 606 1186
Fragments arranged by size:
2318 1186 690 606 138
NgoMIV G'CCGG_C
Cuts at: 0 2092 4938
Size: 2092 2846
NspI r_CATG'y
Cuts at: 0 315 492 649 882 3214 4348 4938
Size: 315 177 157 233 2332 1134 590
Fragments arranged by size:
2332 1134 590 315 233 177 157
PasI CC'CwG_GG
Cuts at: 0 1249 4938
Size: 1249 3689
PciI A'CATG_T
Cuts at: 0 488 4938
Size: 488 4450
PflMI CCAn_nnn'nTGG
Cuts at: 0 1626 1992 2426 3248 3594 4545 4764 4938
Size: 1626 366 434 822 346 951 219 174
Fragments arranged by size:
1626 951 822 434 366 346 219 174
PfoI T'CCnGG_A
Cuts at: 0 77 185 620 1921 4436 4742 4938
Size: 77 108 435 1301 2515 306 196
Fragments arranged by size:
2515 1301 435 306 196 108 77
PmlI CAC'GTG
Cuts at: 0 2166 3237 3567 4938
Size: 2166 1071 330 1371
Fragments arranged by size:
2166 1371 1071 330
PpuMI rG'GwC_Cy
Cuts at: 0 63 1398 2060 2613 2747 3270 3920 4938
Size: 63 1335 662 553 134 523 650 1018
Fragments arranged by size:
1335 1018 662 650 553 523 134 63
PshAI GACnn'nnGTC
Cuts at: 0 2632 4938
Size: 2632 2306
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 730 762 4938
Size: 730 32 4176
Fragments arranged by size:
4176 730 32
PstI C_TGCA'G
Cuts at: 0 53 2051 2399 2798 3080 4913 4938
Size: 53 1998 348 399 282 1833 25
Fragments arranged by size:
1998 1833 399 348 282 53 25
PvuII CAG'CTG
Cuts at: 0 18 699 786 902 1722 2151 2582 2757
Size: 18 681 87 116 820 429 431 175
Cuts at: 2757 2769 3529 4799 4938
Size: 12 760 1270 139
Fragments arranged by size:
1270 820 760 681 431 429 175 139
116 87 18 12
SacI G_AGCT'C
Cuts at: 0 2675 3983 4938
Size: 2675 1308 955
Fragments arranged by size:
2675 1308 955
SapI GCTCTTCn'nnn_
Cuts at: 0 982 1636 2680 3145 3793 4938
Size: 982 654 1044 465 648 1145
Fragments arranged by size:
1145 1044 982 654 648 465
SbfI CC_TGCA'GG
Cuts at: 0 3080 4938
Size: 3080 1858
ScaI AGT'ACT
Cuts at: 0 1378 4938
Size: 1378 3560
SexAI A'CCwGG_T
Cuts at: 0 1225 1400 4938
Size: 1225 175 3538
Fragments arranged by size:
3538 1225 175
SfcI C'TryA_G
Cuts at: 0 49 2047 2395 2794 3076 4909 4938
Size: 49 1998 348 399 282 1833 29
Fragments arranged by size:
1998 1833 399 348 282 49 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2650 3202 3754 4938
Size: 2650 552 552 1184
Fragments arranged by size:
2650 1184 552 552
SfoI GGC'GCC
Cuts at: 0 4477 4938
Size: 4477 461
SmaI CCC'GGG
Cuts at: 0 173 2745 3728 4938
Size: 173 2572 983 1210
Fragments arranged by size:
2572 1210 983 173
SmlI C'TyrA_G
Cuts at: 0 2074 4627 4938
Size: 2074 2553 311
Fragments arranged by size:
2553 2074 311
SphI G_CATG'C
Cuts at: 0 649 4938
Size: 649 4289
StuI AGG'CCT
Cuts at: 0 3322 4711 4938
Size: 3322 1389 227
Fragments arranged by size:
3322 1389 227
StyI C'CwwG_G
Cuts at: 0 577 1315 1433 1874 2036 2318 2456 2702
Size: 577 738 118 441 162 282 138 246
Cuts at: 2702 3086 3146 3752 3986 4664 4758 4832 4938
Size: 384 60 606 234 678 94 74 106
Fragments arranged by size:
738 678 606 577 441 384 282 246
234 162 138 118 106 94 74 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1999 4938
Size: 1999 2939
TatI w'GTAC_w
Cuts at: 0 875 1376 4938
Size: 875 501 3562
Fragments arranged by size:
3562 875 501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 529 561 1780 1812 2035 2067
Size: 198 32 299 32 1219 32 223 32
Cuts at: 2067 3221 3253 4356 4388 4938
Size: 1154 32 1103 32 550
Fragments arranged by size:
1219 1154 1103 550 299 223 198 32
32 32 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 227 1905 1989 2178 2316 3153 3501 3591
Size: 227 1678 84 189 138 837 348 90
Cuts at: 3591 4938
Size: 1347
Fragments arranged by size:
1678 1347 837 348 227 189 138 90
84
XmaI C'CCGG_G
Cuts at: 0 171 2743 3726 4938
Size: 171 2572 983 1212
Fragments arranged by size:
2572 1212 983 171
XmnI GAAnn'nnTTC
Cuts at: 0 3115 3427 4810 4938
Size: 3115 312 1383 128
Fragments arranged by size:
3115 1383 312 128
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AscI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PsrI PstI
PvuII SacI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI ApaI AseI
AsiSI AvrII BaeI BciVI BfrBI BmtI BsiWI BsrGI
BstBI BstZ17I ClaI DraI DrdI EcoRI EcoRV FseI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PsiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI