Restriction map for
pFN21ASDB0062
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI FauI
FbaI FseI FspI HaeII HhaI
HinP1I HpaI KasI KpnI KspI
MaeII MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 1805 GCGG CCGC(-3/-1)
AcyI 58 GGCGTC G[GA]^CG[CT]C
AfaI 439 GTAC GT^AC
AlwNI 1052 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1270 CCTGAGG CC^T[ACGT]AGG
AvaI 1490 CCCGAG C^[CT]CG[GA]G
BanI 837 GGCACC G^G[CT][GA]CC
BbsI 1987 GAAGAC GAAGAC(2/6)
BciVI 1251 GGATAC GTATCC(6/5)
BpuAI 1987 GAAGAC GAAGAC(2/6)
BsaBI 760 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 58 GGCGTC G[GA]^CG[CT]C
BsaI 1889 GGTCTC GGTCTC(1/5)
BsmBI 60 CGTCTC CGTCTC(1/5)
BsmI 1476 GCATTC GAATGC(1/-1)
BsoBI 1490 CCCGAG C^[CT]CG[GA]G
BsrDI 1504 CATTGC GCAATG(2/0)
BstYI 355 GGATCT [GA]^GATC[CT]
Bsu36I 1270 CCTGAGG CC^T[ACGT]AGG
DraI 1044 TTTAAA TTT^AAA
DraII 375 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 235 CCATGG C^C[GA][CT]GG
EaeI 1021 CGGCCA [CT]^GGCC[GA]
Eco81I 1270 CCTGAGG CC^T[ACGT]AGG
EcoO109I 375 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1474 ATGCAT ATGCA^T
HapII 1879 CCGG C^CGG
HgaI 59 GCGTC GACGC(5/10)
Hin1I 58 GGCGTC G[GA]^CG[CT]C
HincII 22 GTTGAC GT[CT]^[GA]AC
HindII 22 GTTGAC GT[CT]^[GA]AC
HindIII 513 AAGCTT A^AGCTT
HpaII 1879 CCGG C^CGG
MamI 760 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 355 GGATCT [GA]^GATC[CT]
MslI 1674 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1803 CAGCGG C[AC]G^C[GT]G
MspI 1879 CCGG C^CGG
MwoI 1591 GCAGCAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 235 CCATGG C^CATGG
NdeI 520 CATATG CA^TATG
NheI 1930 GCTAGC G^CTAGC
NsiI 1474 ATGCAT ATGCA^T
NspI 1295 ACATGC [GA]CATG^[CT]
PpuMI 375 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1838 CTGCAG CTGCA^G
RsaI 439 GTAC GT^AC
SspI 817 AATATT AAT^ATT
XcmI 1119 CCAAGCTATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 355 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 355 GGATC GGATC(4/5)
1519 GGATC
AseI 366 ATTAAT AT^TAAT
451 ATTAAT
AsnI 366 ATTAAT AT^TAAT
451 ATTAAT
AvaII 376 GGTCC G^G[AT]CC
1116 GGTCC
BbvI 140 GCAGC GCAGC(8/12)
1591 GCAGC
BpmI 985 CTCCAG CTGGAG(16/14)
1301 CTGGAG
BseRI 1273 GAGGAG GAGGAG(10/8)
1514 GAGGAG
BsmFI 2005 GGGAC GGGAC(10/14)
913 GTCCC
EcoRV 961 GATATC GAT^ATC
1399 GATATC
Fnu4HI 140 GCAGC GC^[ACGT]GC
1591 GCAGC
HaeIII 122 GGCC GG^CC
1022 GGCC
ItaI 140 GCAGC GC^[ACGT]GC
1591 GCAGC
NlaIV 837 GGCACC GG[ACGT]^[ACGT]CC
1724 GGCTCC
PleI 1210 GACTC GAGTC(4/5)
1902 GAGTC
PshBI 366 ATTAAT AT^TAAT
451 ATTAAT
SexAI 310 ACCTGGT A^CC[AT]GGT
1885 ACCTGGT
TseI 140 GCAGC G^C[AT]GC
1591 GCAGC
XbaI 45 TCTAGA T^CTAGA
930 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI ApoI BfaI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT14I
FokI HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I