(Circular) (Six-base) MAPSORT of: pFN21ASDB0059.seq Check: 8962 from: 1 to: 8140 pFN21ASDB0059 8140 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5950 278 Size: 53 83 186 936 4414 2468 Fragments arranged by size: 4414 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5094 5483 1937 Size: 3157 389 4594 Fragments arranged by size: 4594 3157 389 Acc65I G'GTAC_C Cuts at: 5461 5461 Size: 8140 AclI AA'CG_TT Cuts at: 1563 6267 6640 1563 Size: 4704 373 3063 Fragments arranged by size: 4704 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3412 3731 3941 6203 7587 7855 1632 Size: 1780 319 210 2262 1384 268 1917 Fragments arranged by size: 2262 1917 1780 1384 319 268 210 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7943 AflIII A'CryG_T Cuts at: 1203 7039 8016 1203 Size: 5836 977 1327 Fragments arranged by size: 5836 1327 977 AgeI A'CCGG_T Cuts at: 6971 6971 Size: 8140 AhdI GACnn_n'nnGTC Cuts at: 6869 6869 Size: 8140 AleI CACnn'nnGTG Cuts at: 1166 2895 3419 1166 Size: 1729 524 5887 Fragments arranged by size: 5887 1729 524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8140 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2552 2584 2388 Size: 32 132 32 7944 Fragments arranged by size: 7944 132 32 32 AlwNI CAG_nnn'CTG Cuts at: 2254 2482 2530 3307 3853 4162 4396 4409 5883 Size: 228 48 777 546 309 234 13 1474 Cuts at: 5883 7455 2254 Size: 1572 2939 Fragments arranged by size: 2939 1572 1474 777 546 309 234 228 48 13 ApaI G_GGCC'C Cuts at: 2671 4207 5357 5405 2671 Size: 1536 1150 48 5406 Fragments arranged by size: 5406 1536 1150 48 ApaLI G'TGCA_C Cuts at: 2442 6197 7353 2442 Size: 3755 1156 3229 Fragments arranged by size: 3755 3229 1156 ApoI r'AATT_y Cuts at: 1493 4007 5449 5675 5827 7936 1493 Size: 2514 1442 226 152 2109 1697 Fragments arranged by size: 2514 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 6694 160 Size: 872 5662 1606 Fragments arranged by size: 5662 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8140 AvaI C'yCGr_G Cuts at: 1870 1943 2827 2942 3694 3990 4875 5234 5274 Size: 73 884 115 752 296 885 359 40 Cuts at: 5274 5393 5465 1870 Size: 119 72 4545 Fragments arranged by size: 4545 885 884 752 359 296 119 115 73 72 40 BamHI G'GATC_C Cuts at: 5470 5837 5470 Size: 367 7773 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2938 3041 3227 3698 Size: 325 217 627 354 797 103 186 471 Cuts at: 3698 4292 5312 5426 5461 6916 618 Size: 594 1020 114 35 1455 1842 Fragments arranged by size: 1842 1455 1020 797 627 594 471 354 325 217 186 114 103 35 BanII G_rGCy'C Cuts at: 1391 2035 2671 2878 3404 4207 4420 4595 4812 Size: 644 636 207 526 803 213 175 217 Cuts at: 4812 5357 5405 5459 1391 Size: 545 48 54 4072 Fragments arranged by size: 4072 803 644 636 545 526 217 213 207 175 54 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3247 3945 4417 5286 5312 928 Size: 971 1348 698 472 869 26 3756 Fragments arranged by size: 3756 1348 971 869 698 472 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2158 2192 2261 2295 6331 6365 1903 Size: 34 221 34 69 34 4036 34 3678 Fragments arranged by size: 4036 3678 221 69 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6032 7242 6032 Size: 1210 6930 BclI T'GATC_A Cuts at: 1548 1548 Size: 8140 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 2589 2619 Size: 107 122 71 631 605 506 411 30 Cuts at: 2619 6751 136 Size: 4132 1525 Fragments arranged by size: 4132 1525 631 605 506 411 122 107 71 30 BglII A'GATC_T Cuts at: 3945 8135 3945 Size: 4190 3950 BlpI GC'TnA_GC Cuts at: 2519 2858 5557 2519 Size: 339 2699 5102 Fragments arranged by size: 5102 2699 339 Bme1580I G_kGCm'C Cuts at: 1790 2446 2624 2671 2943 3046 3232 3701 4207 Size: 656 178 47 272 103 186 469 506 Cuts at: 4207 4297 4883 5133 5250 5315 5357 5405 6201 Size: 90 586 250 117 65 42 48 796 Cuts at: 6201 7357 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 796 656 586 506 469 272 250 186 178 117 103 90 65 48 47 42 BmgBI CAC'GTC Cuts at: 1318 2134 1318 Size: 816 7324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3124 5078 5245 Size: 416 53 83 399 24 1707 1954 167 Cuts at: 5245 5341 5871 6829 442 Size: 96 530 958 1753 Fragments arranged by size: 1954 1753 1707 958 530 416 399 167 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5180 1056 Size: 4124 4016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4267 5002 4267 Size: 735 7405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2336 2504 2892 3329 3512 3740 3797 Size: 1068 29 168 388 437 183 228 57 Cuts at: 3797 3843 4103 4376 4691 4790 4997 6800 1239 Size: 46 260 273 315 99 207 1803 2579 Fragments arranged by size: 2579 1803 1068 437 388 315 273 260 228 207 183 168 99 57 46 29 Bpu10I CC'TnA_GC Cuts at: 3659 4319 4779 3659 Size: 660 460 7020 Fragments arranged by size: 7020 660 460 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6265 7130 7428 6265 Size: 865 298 6977 Fragments arranged by size: 6977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3469 4337 6803 882 Size: 617 1970 868 2466 2219 Fragments arranged by size: 2466 2219 1970 868 617 BsaAI yAC'GTr Cuts at: 493 1204 5437 5931 493 Size: 711 4233 494 2702 Fragments arranged by size: 4233 2702 711 494 BsaBI GATnn'nnATC Cuts at: 5412 8134 5412 Size: 2722 5418 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2581 3741 5947 6329 Size: 53 83 186 936 1048 1160 2206 382 Cuts at: 6329 6964 275 Size: 635 1451 Fragments arranged by size: 2206 1451 1160 1048 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3621 6573 6971 7245 7392 7803 3621 Size: 2952 398 274 147 411 3958 Fragments arranged by size: 3958 2952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2372 2388 2402 2418 3429 Size: 30 165 30 969 16 14 16 1011 Cuts at: 3429 3459 1178 Size: 30 5859 Fragments arranged by size: 5859 1011 969 165 30 30 30 16 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2333 2648 4994 5315 1186 Size: 1147 315 2346 321 4011 Fragments arranged by size: 4011 2346 1147 321 315 BseYI C'CCAG_C Cuts at: 3073 3119 3566 3890 3897 4114 4119 4155 4270 Size: 46 447 324 7 217 5 36 115 Cuts at: 4270 4467 4811 5162 5398 7343 7839 3073 Size: 197 344 351 236 1945 496 3374 Fragments arranged by size: 3374 1945 496 447 351 344 324 236 217 197 115 46 36 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2180 2868 4169 4395 1163 Size: 573 444 688 1301 226 4908 Fragments arranged by size: 4908 1301 688 573 444 226 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5590 6351 7379 1540 Size: 275 186 3589 761 1028 2301 Fragments arranged by size: 3589 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2245 2446 2520 3426 6201 6286 7357 2245 Size: 201 74 906 2775 85 1071 3028 Fragments arranged by size: 3028 2775 1071 906 201 85 74 BsmI GAATG_Cn' Cuts at: 4252 5651 5744 4252 Size: 1399 93 6648 Fragments arranged by size: 6648 1399 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2480 3732 4961 2480 Size: 1252 1229 5659 Fragments arranged by size: 5659 1252 1229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2245 2446 2520 2624 2671 2878 Size: 399 245 210 201 74 104 47 207 Cuts at: 2878 2943 3046 3232 3404 3426 3701 4207 4297 Size: 65 103 186 172 22 275 506 90 Cuts at: 4297 4420 4595 4812 4883 5133 5250 5315 5357 Size: 123 175 217 71 250 117 65 42 Cuts at: 5357 5405 5459 6201 6286 7357 1391 Size: 48 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 506 399 275 250 245 217 210 207 201 186 175 172 123 117 104 103 90 85 74 71 65 65 54 48 47 42 22 BspEI T'CCGG_A Cuts at: 7803 7803 Size: 8140 BspHI T'CATG_A Cuts at: 4621 6029 7759 4621 Size: 1408 1730 5002 Fragments arranged by size: 5002 1730 1408 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3399 4110 4651 5495 844 Size: 2555 711 541 844 3489 Fragments arranged by size: 3489 2555 844 711 541 BsrBI CCG'CTC Cuts at: 1394 1823 4522 4801 6027 1394 Size: 429 2699 279 1226 3507 Fragments arranged by size: 3507 2699 1226 429 279 BsrDI GCAATG_nn' Cuts at: 65 1248 3555 4446 6635 6809 65 Size: 1183 2307 891 2189 174 1396 Fragments arranged by size: 2307 2189 1396 1183 891 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2199 2766 6784 6971 1700 Size: 110 389 567 4018 187 2869 Fragments arranged by size: 4018 2869 567 389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8140 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8140 BssSI C'ACGA_G Cuts at: 4413 6200 7212 4413 Size: 1787 1012 5341 Fragments arranged by size: 5341 1787 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3227 4396 794 Size: 2433 1169 4538 Fragments arranged by size: 4538 2433 1169 BstBI TT'CG_AA Cuts at: 2007 6944 2007 Size: 4937 3203 BstEII G'GTnAC_C Cuts at: 1184 4093 4300 1184 Size: 2909 207 5024 Fragments arranged by size: 5024 2909 207 BstXI CCAn_nnnn'nTGG Cuts at: 2420 2935 4030 4133 4354 4894 5113 2420 Size: 515 1095 103 221 540 219 5447 Fragments arranged by size: 5447 1095 540 515 221 219 103 BstYI r'GATC_y Cuts at: 1972 2478 3123 3909 3945 5470 5837 6222 6239 Size: 506 645 786 36 1525 367 385 17 Cuts at: 6239 7691 7777 7789 8135 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1525 1452 786 645 506 385 367 346 86 36 17 12 BstZ17I GTA'TAC Cuts at: 5095 5095 Size: 8140 Bsu36I CC'TnA_GG Cuts at: 3536 5321 3536 Size: 1785 6355 BtgI C'CryG_G Cuts at: 513 1065 2022 3265 4023 4347 5106 513 Size: 552 957 1243 758 324 759 3547 Fragments arranged by size: 3547 1243 957 759 758 552 324 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7401 Fragments arranged by size: 7401 683 56 BtsI GCAGTG_nn' Cuts at: 3228 3426 4181 5180 5656 6450 6470 3228 Size: 198 755 999 476 794 20 4898 Fragments arranged by size: 4898 999 794 755 476 198 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8105 DraI TTT'AAA Cuts at: 5444 5444 Size: 8140 DraIII CAC_nnn'GTG Cuts at: 3705 4061 3705 Size: 356 7784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3539 7147 809 Size: 507 2223 3608 1802 Fragments arranged by size: 3608 2223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2532 3023 Size: 54 1425 50 275 18 195 507 491 Cuts at: 3023 3514 3844 4374 4458 4826 4884 5587 6476 Size: 491 330 530 84 368 58 703 889 Cuts at: 6476 8105 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 703 530 507 491 491 368 330 275 195 84 58 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5587 1537 Size: 275 3775 4090 Fragments arranged by size: 4090 3775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2180 2265 2300 2847 3063 3431 4280 Size: 755 72 85 35 547 216 368 849 Cuts at: 4280 4481 6070 1353 Size: 201 1589 3423 Fragments arranged by size: 3423 1589 849 755 547 368 216 201 85 72 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6727 7101 7247 387 Size: 1064 138 5138 374 146 1280 Fragments arranged by size: 5138 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2336 2504 2892 3329 3412 3512 Size: 393 675 29 168 388 437 83 100 Cuts at: 3512 3731 3740 3797 3843 3941 4103 4376 4691 Size: 219 9 57 46 98 162 273 315 Cuts at: 4691 4790 4997 6203 6800 7587 7855 1239 Size: 99 207 1206 597 787 268 1524 Fragments arranged by size: 1524 1206 787 675 597 437 393 388 315 273 268 219 207 168 162 100 99 98 83 57 46 29 9 EcoNI CCTnn'n_nnAGG Cuts at: 2915 2915 Size: 8140 EcoO109I rG'GnC_Cy Cuts at: 2055 2251 2417 2527 2667 2668 3190 4204 4685 Size: 196 166 110 140 1 522 1014 481 Cuts at: 4685 5353 5402 2055 Size: 668 49 4793 Fragments arranged by size: 4793 1014 668 522 481 196 166 140 110 49 1 EcoRI G'AATT_C Cuts at: 4007 5449 5827 4007 Size: 1442 378 6320 Fragments arranged by size: 6320 1442 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3794 3794 Size: 8140 FspI TGC'GCA Cuts at: 2150 2915 6646 2150 Size: 765 3731 3644 Fragments arranged by size: 3731 3644 765 HaeII r_GCGC'y Cuts at: 1678 2928 2968 3064 7287 1678 Size: 1250 40 96 4223 2531 Fragments arranged by size: 4223 2531 1250 96 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2328 2360 3115 3147 3429 Size: 32 135 32 756 32 755 32 282 Cuts at: 3429 3461 4407 4439 6781 6813 6855 6887 1373 Size: 32 946 32 2342 32 42 32 2626 Fragments arranged by size: 2626 2342 946 756 755 282 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5484 5730 669 Size: 988 281 3546 246 3079 Fragments arranged by size: 3546 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3776 748 Size: 3028 5112 HpaI GTT'AAC Cuts at: 5730 5730 Size: 8140 KpnI G_GTAC'C Cuts at: 5465 5465 Size: 8140 MfeI C'AATT_G Cuts at: 5739 5739 Size: 8140 MluI A'CGCG_T Cuts at: 8016 8016 Size: 8140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 4036 4587 7254 7438 1305 Size: 1224 1507 551 2667 184 2007 Fragments arranged by size: 2667 2007 1507 1224 551 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 3025 3846 4376 4460 Size: 54 1425 343 702 491 821 530 84 Cuts at: 4460 4828 10 Size: 368 3322 Fragments arranged by size: 3322 1425 821 702 530 491 368 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2451 2895 3419 3720 3791 4028 4734 Size: 648 1285 444 524 301 71 237 706 Cuts at: 4734 5030 5111 6098 6457 6616 518 Size: 296 81 987 359 159 2042 Fragments arranged by size: 2042 1285 987 706 648 524 444 359 301 296 237 159 81 71 MspA1I CmG'CkG Cuts at: 1606 2627 2737 3901 3967 4905 4991 5556 6233 Size: 1021 110 1164 66 938 86 565 677 Cuts at: 6233 7381 7626 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1164 1148 1021 938 677 565 245 110 86 66 NcoI C'CATG_G Cuts at: 513 1065 2022 3265 4023 5106 513 Size: 552 957 1243 758 1083 3547 Fragments arranged by size: 3547 1243 1083 957 758 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8140 NheI G'CTAG_C Cuts at: 1052 5176 1052 Size: 4124 4016 NotI GC'GGCC_GC Cuts at: 5587 5587 Size: 8140 NspI r_CATG'y Cuts at: 1132 1229 4819 4946 5498 7043 1132 Size: 97 3590 127 552 1545 2229 Fragments arranged by size: 3590 2229 1545 552 127 97 PasI CC'CwG_GG Cuts at: 1348 4588 1348 Size: 3240 4900 PciI A'CATG_T Cuts at: 7039 7039 Size: 8140 PflMI CCAn_nnn'nTGG Cuts at: 1108 2254 2392 2482 2530 4076 4162 1108 Size: 1146 138 90 48 1546 86 5086 Fragments arranged by size: 5086 1546 1146 138 90 86 48 PfoI T'CCnGG_A Cuts at: 2060 2481 2538 3756 4328 2060 Size: 421 57 1218 572 5872 Fragments arranged by size: 5872 1218 572 421 57 PmeI GTTT'AAAC Cuts at: 5444 5444 Size: 8140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2388 2420 6209 6241 7722 7754 2388 Size: 32 3789 32 1481 32 2774 Fragments arranged by size: 3789 2774 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2527 3190 2527 Size: 663 7477 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8140 PsiI TTA'TAA Cuts at: 5710 5710 Size: 8140 PspOMI G'GGCC_C Cuts at: 2667 4203 5353 5401 2667 Size: 1536 1150 48 5406 Fragments arranged by size: 5406 1536 1150 48 PstI C_TGCA'G Cuts at: 830 3966 5492 830 Size: 3136 1526 3478 Fragments arranged by size: 3478 3136 1526 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8140 PvuII CAG'CTG Cuts at: 2627 2737 3967 4905 4991 2627 Size: 110 1230 938 86 5776 Fragments arranged by size: 5776 1230 938 110 86 SalI G'TCGA_C Cuts at: 1936 5482 1936 Size: 3546 4594 SapI GCTCTTCn'nnn_ Cuts at: 2108 2265 2847 3063 3431 2108 Size: 157 582 216 368 6817 Fragments arranged by size: 6817 582 368 216 157 SbfI CC_TGCA'GG Cuts at: 5492 5492 Size: 8140 ScaI AGT'ACT Cuts at: 4985 6388 8100 4985 Size: 1403 1712 5025 Fragments arranged by size: 5025 1712 1403 SfcI C'TryA_G Cuts at: 826 1045 2332 3574 3805 3962 4915 5488 6623 Size: 219 1287 1242 231 157 953 573 1135 Cuts at: 6623 7304 7495 826 Size: 681 191 1471 Fragments arranged by size: 1471 1287 1242 1135 953 681 573 231 219 191 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8140 SmaI CCC'GGG Cuts at: 1872 3992 4877 5467 1872 Size: 2120 885 590 4545 Fragments arranged by size: 4545 2120 885 590 SmlI C'TyrA_G Cuts at: 820 1017 1943 6244 7145 7407 820 Size: 197 926 4301 901 262 1553 Fragments arranged by size: 4301 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5437 5931 493 Size: 4944 494 2702 Fragments arranged by size: 4944 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8140 SphI G_CATG'C Cuts at: 1132 5498 1132 Size: 4366 3774 SspI AAT'ATT Cuts at: 5 52 6064 5 Size: 47 6012 2081 Fragments arranged by size: 6012 2081 47 StuI AGG'CCT Cuts at: 1517 2292 3692 1517 Size: 775 1400 5965 Fragments arranged by size: 5965 1400 775 StyI C'CwwG_G Cuts at: 513 1065 2022 2048 2587 3265 4023 4567 5106 Size: 552 957 26 539 678 758 544 539 Cuts at: 5106 5579 513 Size: 473 3074 Fragments arranged by size: 3074 957 758 678 552 544 539 539 473 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2228 3029 3215 3511 3741 3911 Size: 1255 549 99 801 186 296 230 170 Cuts at: 3911 5298 6167 6184 6337 6522 6967 325 Size: 1387 869 17 153 185 445 1498 Fragments arranged by size: 1498 1387 1255 869 801 549 445 296 230 186 185 170 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3093 4983 5155 Size: 274 80 33 51 1443 1116 1890 172 Cuts at: 5155 6386 8098 96 Size: 1231 1712 138 Fragments arranged by size: 1890 1712 1443 1231 1116 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2372 2404 2557 2589 5355 5387 8100 8132 2372 Size: 32 153 32 2766 32 2713 32 2380 Fragments arranged by size: 2766 2713 2380 153 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3728 3799 1316 Size: 2412 71 5657 Fragments arranged by size: 5657 2412 71 XbaI T'CTAG_A Cuts at: 5476 5476 Size: 8140 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2600 4453 5113 2600 Size: 1853 660 5627 Fragments arranged by size: 5627 1853 660 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8140 XmaI C'CCGG_G Cuts at: 1870 3990 4875 5465 1870 Size: 2120 885 590 4545 Fragments arranged by size: 4545 2120 885 590 XmnI GAAnn'nnTTC Cuts at: 3254 6269 3254 Size: 3015 5125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5948 276 Size: 53 83 186 936 4414 2468 Fragments arranged by size: 4414 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI