Restriction map for
pFN21ASDB0059
(Linear) (Six-base) MAPSORT of: KIBB0059.seq Check: 1657 from: 1 to: 3411
KIBB0059.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AccI GT'mk_AC
Cuts at: 0 3071 3411
Size: 3071 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1708 1918 3411
Size: 1389 319 210 1493
Fragments arranged by size:
1493 1389 319 210
AleI CACnn'nnGTG
Cuts at: 0 872 1396 3411
Size: 872 524 2015
Fragments arranged by size:
2015 872 524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 365 397 529 561 3411
Size: 365 32 132 32 2850
Fragments arranged by size:
2850 365 132 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 231 459 507 1284 1830 2139 2373 2386
Size: 231 228 48 777 546 309 234 13
Cuts at: 2386 3411
Size: 1025
Fragments arranged by size:
1025 777 546 309 234 231 228 48
13
ApaI G_GGCC'C
Cuts at: 0 648 2184 3334 3382 3411
Size: 648 1536 1150 48 29
Fragments arranged by size:
1536 1150 648 48 29
ApaLI G'TGCA_C
Cuts at: 0 419 3411
Size: 419 2992
ApoI r'AATT_y
Cuts at: 0 1984 3411
Size: 1984 1427
AvaI C'yCGr_G
Cuts at: 0 804 919 1671 1967 2852 3211 3251 3370
Size: 804 115 752 296 885 359 40 119
Cuts at: 3370 3411
Size: 41
Fragments arranged by size:
885 804 752 359 296 119 115 41
40
BanI G'GyrC_C
Cuts at: 0 118 915 1018 1204 1675 2269 3289 3403
Size: 118 797 103 186 471 594 1020 114
Cuts at: 3403 3411
Size: 8
Fragments arranged by size:
1020 797 594 471 186 118 114 103
8
BanII G_rGCy'C
Cuts at: 0 12 648 855 1381 2184 2397 2572 2789
Size: 12 636 207 526 803 213 175 217
Cuts at: 2789 3334 3382 3411
Size: 545 48 29
Fragments arranged by size:
803 636 545 526 217 213 207 175
48 29 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 1224 1922 2394 3263 3289 3411
Size: 1224 698 472 869 26 122
Fragments arranged by size:
1224 869 698 472 122 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 135 169 238 272 3411
Size: 135 34 69 34 3139
Fragments arranged by size:
3139 135 69 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 155 566 596 3411
Size: 155 411 30 2815
Fragments arranged by size:
2815 411 155 30
BglII A'GATC_T
Cuts at: 0 1922 3411
Size: 1922 1489
BlpI GC'TnA_GC
Cuts at: 0 496 835 3411
Size: 496 339 2576
Fragments arranged by size:
2576 496 339
Bme1580I G_kGCm'C
Cuts at: 0 423 601 648 920 1023 1209 1678 2184
Size: 423 178 47 272 103 186 469 506
Cuts at: 2184 2274 2860 3110 3227 3292 3334 3382 3411
Size: 90 586 250 117 65 42 48 29
Fragments arranged by size:
586 506 469 423 272 250 186 178
117 103 90 65 48 47 42 29
BmgBI CAC'GTC
Cuts at: 0 111 3411
Size: 111 3300
BmrI ACTGGGnnnn_n'
Cuts at: 0 1101 3055 3222 3318 3411
Size: 1101 1954 167 96 93
Fragments arranged by size:
1954 1101 167 96 93
BmtI G_CTAG'C
Cuts at: 0 3157 3411
Size: 3157 254
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2244 2979 3411
Size: 2244 735 432
Fragments arranged by size:
2244 735 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 313 481 869 1306 1489 1717 1774
Size: 284 29 168 388 437 183 228 57
Cuts at: 1774 1820 2080 2353 2668 2767 2974 3411
Size: 46 260 273 315 99 207 437
Fragments arranged by size:
437 437 388 315 284 273 260 228
207 183 168 99 57 46 29
Bpu10I CC'TnA_GC
Cuts at: 0 1636 2296 2756 3411
Size: 1636 660 460 655
Fragments arranged by size:
1636 660 655 460
BsaI GGTCTCn'nnnn_
Cuts at: 0 1446 2314 3411
Size: 1446 868 1097
Fragments arranged by size:
1446 1097 868
BsaBI GATnn'nnATC
Cuts at: 0 3389 3411
Size: 3389 22
BsaHI Gr'CG_yC
Cuts at: 0 558 1718 3411
Size: 558 1160 1693
Fragments arranged by size:
1693 1160 558
BsaWI w'CCGG_w
Cuts at: 0 1598 3411
Size: 1598 1813
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 365 379 395 1406 1436 3411
Size: 349 16 14 16 1011 30 1975
Fragments arranged by size:
1975 1011 349 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 625 2971 3292 3411
Size: 310 315 2346 321 119
Fragments arranged by size:
2346 321 315 310 119
BseYI C'CCAG_C
Cuts at: 0 1050 1096 1543 1867 1874 2091 2096 2132
Size: 1050 46 447 324 7 217 5 36
Cuts at: 2132 2247 2444 2788 3139 3375 3411
Size: 115 197 344 351 236 36
Fragments arranged by size:
1050 447 351 344 324 236 217 197
115 46 36 36 7 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 845 2146 2372 3411
Size: 157 688 1301 226 1039
Fragments arranged by size:
1301 1039 688 226 157
BsiHKAI G_wGCw'C
Cuts at: 0 222 423 497 1403 3411
Size: 222 201 74 906 2008
Fragments arranged by size:
2008 906 222 201 74
BsmI GAATG_Cn'
Cuts at: 0 2229 3411
Size: 2229 1182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 1709 2938 3411
Size: 457 1252 1229 473
Fragments arranged by size:
1252 1229 473 457
Bsp1286I G_dGCh'C
Cuts at: 0 12 222 423 497 601 648 855 920
Size: 12 210 201 74 104 47 207 65
Cuts at: 920 1023 1209 1381 1403 1678 2184 2274 2397
Size: 103 186 172 22 275 506 90 123
Cuts at: 2397 2572 2789 2860 3110 3227 3292 3334 3382
Size: 175 217 71 250 117 65 42 48
Cuts at: 3382 3411
Size: 29
Fragments arranged by size:
506 275 250 217 210 207 201 186
175 172 123 117 104 103 90 74
71 65 65 48 47 42 29 22
12
BspHI T'CATG_A
Cuts at: 0 2598 3411
Size: 2598 813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1376 2087 2628 3411
Size: 1376 711 541 783
Fragments arranged by size:
1376 783 711 541
BsrBI CCG'CTC
Cuts at: 0 2499 2778 3411
Size: 2499 279 633
Fragments arranged by size:
2499 633 279
BsrDI GCAATG_nn'
Cuts at: 0 1532 2423 3411
Size: 1532 891 988
Fragments arranged by size:
1532 988 891
BsrFI r'CCGG_y
Cuts at: 0 176 743 3411
Size: 176 567 2668
Fragments arranged by size:
2668 567 176
BssSI C'ACGA_G
Cuts at: 0 2390 3411
Size: 2390 1021
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1204 2373 3411
Size: 1204 1169 1038
Fragments arranged by size:
1204 1169 1038
BstEII G'GTnAC_C
Cuts at: 0 2070 2277 3411
Size: 2070 207 1134
Fragments arranged by size:
2070 1134 207
BstXI CCAn_nnnn'nTGG
Cuts at: 0 397 912 2007 2110 2331 2871 3090 3411
Size: 397 515 1095 103 221 540 219 321
Fragments arranged by size:
1095 540 515 397 321 221 219 103
BstYI r'GATC_y
Cuts at: 0 455 1100 1886 1922 3411
Size: 455 645 786 36 1489
Fragments arranged by size:
1489 786 645 455 36
BstZ17I GTA'TAC
Cuts at: 0 3072 3411
Size: 3072 339
Bsu36I CC'TnA_GG
Cuts at: 0 1513 3298 3411
Size: 1513 1785 113
Fragments arranged by size:
1785 1513 113
BtgI C'CryG_G
Cuts at: 0 1242 2000 2324 3083 3411
Size: 1242 758 324 759 328
Fragments arranged by size:
1242 759 758 328 324
BtsI GCAGTG_nn'
Cuts at: 0 1205 1403 2158 3157 3411
Size: 1205 198 755 999 254
Fragments arranged by size:
1205 999 755 254 198
DraIII CAC_nnn'GTG
Cuts at: 0 1682 2038 3411
Size: 1682 356 1373
Fragments arranged by size:
1682 1373 356
DrdI GACnn_nn'nnGTC
Cuts at: 0 1516 3411
Size: 1516 1895
EaeI y'GGCC_r
Cuts at: 0 2 509 1000 1491 1821 2351 2435 2803
Size: 2 507 491 491 330 530 84 368
Cuts at: 2803 2861 3411
Size: 58 550
Fragments arranged by size:
550 530 507 491 491 368 330 84
58 2
EarI CTCTTCn'nnn_
Cuts at: 0 85 157 242 277 824 1040 1408 2257
Size: 85 72 85 35 547 216 368 849
Cuts at: 2257 2458 3411
Size: 201 953
Fragments arranged by size:
953 849 547 368 216 201 85 85
72 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 313 481 869 1306 1389 1489 1708
Size: 284 29 168 388 437 83 100 219
Cuts at: 1708 1717 1774 1820 1918 2080 2353 2668 2767
Size: 9 57 46 98 162 273 315 99
Cuts at: 2767 2974 3411
Size: 207 437
Fragments arranged by size:
437 437 388 315 284 273 219 207
168 162 100 99 98 83 57 46
29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 892 3411
Size: 892 2519
EcoO109I rG'GnC_Cy
Cuts at: 0 32 228 394 504 644 645 1167 2181
Size: 32 196 166 110 140 1 522 1014
Cuts at: 2181 2662 3330 3379 3411
Size: 481 668 49 32
Fragments arranged by size:
1014 668 522 481 196 166 140 110
49 32 32 1
EcoRI G'AATT_C
Cuts at: 0 1984 3411
Size: 1984 1427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1771 3411
Size: 1771 1640
FspI TGC'GCA
Cuts at: 0 127 892 3411
Size: 127 765 2519
Fragments arranged by size:
2519 765 127
HaeII r_GCGC'y
Cuts at: 0 905 945 1041 3411
Size: 905 40 96 2370
Fragments arranged by size:
2370 905 96 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 305 337 1092 1124 1406 1438 2384 2416
Size: 305 32 755 32 282 32 946 32
Cuts at: 2416 3411
Size: 995
Fragments arranged by size:
995 946 755 305 282 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1753 3411
Size: 1753 1658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 2013 2564 3411
Size: 506 1507 551 847
Fragments arranged by size:
1507 847 551 506
MscI TGG'CCA
Cuts at: 0 511 1002 1823 2353 2437 2805 3411
Size: 511 491 821 530 84 368 606
Fragments arranged by size:
821 606 530 511 491 368 84
MslI CAynn'nnrTG
Cuts at: 0 428 872 1396 1697 1768 2005 2711 3007
Size: 428 444 524 301 71 237 706 296
Cuts at: 3007 3088 3411
Size: 81 323
Fragments arranged by size:
706 524 444 428 323 301 296 237
81 71
MspA1I CmG'CkG
Cuts at: 0 604 714 1878 1944 2882 2968 3411
Size: 604 110 1164 66 938 86 443
Fragments arranged by size:
1164 938 604 443 110 86 66
NcoI C'CATG_G
Cuts at: 0 1242 2000 3083 3411
Size: 1242 758 1083 328
Fragments arranged by size:
1242 1083 758 328
NheI G'CTAG_C
Cuts at: 0 3153 3411
Size: 3153 258
NspI r_CATG'y
Cuts at: 0 2796 2923 3411
Size: 2796 127 488
Fragments arranged by size:
2796 488 127
PasI CC'CwG_GG
Cuts at: 0 2565 3411
Size: 2565 846
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 369 459 507 2053 2139 3411
Size: 231 138 90 48 1546 86 1272
Fragments arranged by size:
1546 1272 231 138 90 86 48
PfoI T'CCnGG_A
Cuts at: 0 37 458 515 1733 2305 3411
Size: 37 421 57 1218 572 1106
Fragments arranged by size:
1218 1106 572 421 57 37
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 365 397 3411
Size: 365 32 3014
Fragments arranged by size:
3014 365 32
PpuMI rG'GwC_Cy
Cuts at: 0 504 1167 3411
Size: 504 663 2244
Fragments arranged by size:
2244 663 504
PspOMI G'GGCC_C
Cuts at: 0 644 2180 3330 3378 3411
Size: 644 1536 1150 48 33
Fragments arranged by size:
1536 1150 644 48 33
PstI C_TGCA'G
Cuts at: 0 1943 3411
Size: 1943 1468
PvuII CAG'CTG
Cuts at: 0 604 714 1944 2882 2968 3411
Size: 604 110 1230 938 86 443
Fragments arranged by size:
1230 938 604 443 110 86
SapI GCTCTTCn'nnn_
Cuts at: 0 85 242 824 1040 1408 3411
Size: 85 157 582 216 368 2003
Fragments arranged by size:
2003 582 368 216 157 85
ScaI AGT'ACT
Cuts at: 0 2962 3411
Size: 2962 449
SfcI C'TryA_G
Cuts at: 0 309 1551 1782 1939 2892 3411
Size: 309 1242 231 157 953 519
Fragments arranged by size:
1242 953 519 309 231 157
SmaI CCC'GGG
Cuts at: 0 1969 2854 3411
Size: 1969 885 557
Fragments arranged by size:
1969 885 557
StuI AGG'CCT
Cuts at: 0 269 1669 3411
Size: 269 1400 1742
Fragments arranged by size:
1742 1400 269
StyI C'CwwG_G
Cuts at: 0 25 564 1242 2000 2544 3083 3411
Size: 25 539 678 758 544 539 328
Fragments arranged by size:
758 678 544 539 539 328 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 205 1006 1192 1488 1718 1888 3275
Size: 106 99 801 186 296 230 170 1387
Cuts at: 3275 3411
Size: 136
Fragments arranged by size:
1387 801 296 230 186 170 136 106
99
TatI w'GTAC_w
Cuts at: 0 1070 2960 3132 3411
Size: 1070 1890 172 279
Fragments arranged by size:
1890 1070 279 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 349 381 534 566 3332 3364 3411
Size: 349 32 153 32 2766 32 47
Fragments arranged by size:
2766 349 153 47 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1705 1776 3411
Size: 1705 71 1635
Fragments arranged by size:
1705 1635 71
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 577 2430 3090 3411
Size: 577 1853 660 321
Fragments arranged by size:
1853 660 577 321
XmaI C'CCGG_G
Cuts at: 0 1967 2852 3411
Size: 1967 885 559
Fragments arranged by size:
1967 885 559
XmnI GAAnn'nnTTC
Cuts at: 0 1231 3411
Size: 1231 2180
Enzymes that do cut:
AccI AcuI AleI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbsI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NheI NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SapI ScaI SfcI
SmaI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BciVI BclI BfrBI BpuEI BsaAI BsiEI
BsiWI BspEI BsrGI BssHII BstBI BtgZI ClaI CspCI
DraI EagI EciI EcoICRI EcoRV FseI FspAI HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI