Restriction map for pFN21ASDB0033
 (Linear) (Six-base) MAPSORT of: KIBB0033.seq  Check: 8481  from: 1  to: 3189



KIBB0033.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2520    3189 
   Size:       2520     669
 
Acc65I G'GTAC_C

Cuts at:      0      21    3189 
   Size:         21    3168
 
AflII C'TTAA_G

Cuts at:      0    1708    3189 
   Size:       1708    1481
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     485     495     553    3189 
   Size:        485      10      58    2636

  Fragments arranged by size: 

                2636     485      58      10
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     565     597    3189 
   Size:        565      32    2592

  Fragments arranged by size: 

                2592     565      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      65     729    2208    2610    2820    3189 
   Size:         65     664    1479     402     210     369

  Fragments arranged by size: 

                1479     664     402     369     210      65
 
ApaI G_GGCC'C

Cuts at:      0    3085    3189 
   Size:       3085     104
 
ApaLI G'TGCA_C

Cuts at:      0     682    3189 
   Size:        682    2507
 
ApoI r'AATT_y

Cuts at:      0    1621    2257    3180    3189 
   Size:       1621     636     923       9

  Fragments arranged by size: 

                1621     923     636       9
 
AvaI C'yCGr_G

Cuts at:      0     490    1249    1501    2407    2723    3189 
   Size:        490     759     252     906     316     466

  Fragments arranged by size: 

                 906     759     490     466     316     252
 
BanI G'GyrC_C

Cuts at:      0      21     502     628    2766    3097    3152    3189 
   Size:         21     481     126    2138     331      55      37

  Fragments arranged by size: 

                2138     481     331     126      55      37      21
 
BanII G_rGCy'C

Cuts at:      0    1094    1102    1225    1256    1355    1409    1772    1859 
   Size:       1094       8     123      31      99      54     363      87

Cuts at:   1859    2746    2753    3085    3189 
   Size:        887       7     332     104

  Fragments arranged by size: 

                1094     887     363     332     123     104      99      87
                  54      31       8       7
 
BbvCI CC'TCA_GC

Cuts at:      0    1024    3189 
   Size:       1024    2165
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1536    3189 
   Size:       1536    1653
 
BglII A'GATC_T

Cuts at:      0     281    2141    2636    3189 
   Size:        281    1860     495     553

  Fragments arranged by size: 

                1860     553     495     281
 
BlpI GC'TnA_GC

Cuts at:      0     913    3189 
   Size:        913    2276
 
Bme1580I G_kGCm'C

Cuts at:      0     338     633     686    2038    3085    3155    3189 
   Size:        338     295      53    1352    1047      70      34

  Fragments arranged by size: 

                1352    1047     338     295      70      53      34
 
BmgBI CAC'GTC

Cuts at:      0    1215    2045    3189 
   Size:       1215     830    1144

  Fragments arranged by size: 

                1215    1144     830
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1800    2323    3189 
   Size:       1800     523     866

  Fragments arranged by size: 

                1800     866     523
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1156    3189 
   Size:       1156    2033
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     562     567     751    1132    1549    1558    2209 
   Size:        181     381       5     184     381     417       9     651

Cuts at:   2209    2253    3189 
   Size:         44     936

  Fragments arranged by size: 

                 936     651     417     381     381     184     181      44
                   9       5
 
Bpu10I CC'TnA_GC

Cuts at:      0    1024    1096    2914    3189 
   Size:       1024      72    1818     275

  Fragments arranged by size: 

                1818    1024     275      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     988    1345    3189 
   Size:        988     357    1844

  Fragments arranged by size: 

                1844     988     357
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     258     469    1708    3189 
   Size:        258     211    1239    1481

  Fragments arranged by size: 

                1481    1239     258     211
 
BsaAI yAC'GTr

Cuts at:      0    2054    2994    3189 
   Size:       2054     940     195

  Fragments arranged by size: 

                2054     940     195
 
BsaBI GATnn'nnATC

Cuts at:      0    2146    3189 
   Size:       2146    1043
 
BsaHI Gr'CG_yC

Cuts at:      0     317    1136    2855    3189 
   Size:        317     819    1719     334

  Fragments arranged by size: 

                1719     819     334     317
 
BsaWI w'CCGG_w

Cuts at:      0     569    1700    1760    2893    3189 
   Size:        569    1131      60    1133     296

  Fragments arranged by size: 

                1133    1131     569     296      60
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     491     521     565     595    1441    1471    1705    1735 
   Size:        491      30      44      30     846      30     234      30

Cuts at:   1735    2960    2990    3189 
   Size:       1225      30     199

  Fragments arranged by size: 

                1225     846     491     234     199      44      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     757     760     816     982    1012    1102    1390    1465 
   Size:        757       3      56     166      30      90     288      75

Cuts at:   1465    1501    3189 
   Size:         36    1688

  Fragments arranged by size: 

                1688     757     288     166      90      75      56      36
                  30       3
 
BseYI C'CCAG_C

Cuts at:      0     483     762    1225    3189 
   Size:        483     279     463    1964

  Fragments arranged by size: 

                1964     483     463     279
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     631    1618    3189 
   Size:        163     468     987    1571

  Fragments arranged by size: 

                1571     987     468     163
 
BsiEI CG_ry'CG

Cuts at:      0    1799    1905    2068    2787    3189 
   Size:       1799     106     163     719     402

  Fragments arranged by size: 

                1799     719     402     163     106
 
BsiHKAI G_wGCw'C

Cuts at:      0     378     686    1094    1240    1355    1772    3189 
   Size:        378     308     408     146     115     417    1417

  Fragments arranged by size: 

                1417     417     408     378     308     146     115
 
BsiWI C'GTAC_G

Cuts at:      0    1124    3189 
   Size:       1124    2065
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1127    1786    2035    2995    3189 
   Size:       1127     659     249     960     194

  Fragments arranged by size: 

                1127     960     659     249     194
 
Bsp1286I G_dGCh'C

Cuts at:      0     338     378     633     686    1094    1102    1225    1240 
   Size:        338      40     255      53     408       8     123      15

Cuts at:   1240    1256    1355    1409    1772    1859    2038    2746    2753 
   Size:         16      99      54     363      87     179     708       7

Cuts at:   2753    3085    3155    3189 
   Size:        332      70      34

  Fragments arranged by size: 

                 708     408     363     338     332     255     179     123
                  99      87      70      54      53      40      34      16
                  15       8       7
 
BspEI T'CCGG_A

Cuts at:      0     569    1700    3189 
   Size:        569    1131    1489

  Fragments arranged by size: 

                1489    1131     569
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     206     284     487     578     917    1970    2520    3189 
   Size:        206      78     203      91     339    1053     550     669

  Fragments arranged by size: 

                1053     669     550     339     206     203      91      78
            
 
BsrBI CCG'CTC

Cuts at:      0     425     702    3189 
   Size:        425     277    2487

  Fragments arranged by size: 

                2487     425     277
 
BsrDI GCAATG_nn'

Cuts at:      0     981    3189 
   Size:        981    2208
 
BsrFI r'CCGG_y

Cuts at:      0     290     515    1978    3189 
   Size:        290     225    1463    1211

  Fragments arranged by size: 

                1463    1211     290     225
 
BsrGI T'GTAC_A

Cuts at:      0    1154    2940    3189 
   Size:       1154    1786     249

  Fragments arranged by size: 

                1786    1154     249
 
BssSI C'ACGA_G

Cuts at:      0     674    1130    2493    3189 
   Size:        674     456    1363     696

  Fragments arranged by size: 

                1363     696     674     456
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2610    2820    3189 
   Size:       2610     210     369

  Fragments arranged by size: 

                2610     369     210
 
BstEII G'GTnAC_C

Cuts at:      0      99     984    1650    3189 
   Size:         99     885     666    1539

  Fragments arranged by size: 

                1539     885     666      99
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     694    1215    2045    3189 
   Size:        694     521     830    1144

  Fragments arranged by size: 

                1144     830     694     521
 
BstYI r'GATC_y

Cuts at:      0     281    1838    2141    2219    2636    3189 
   Size:        281    1557     303      78     417     553

  Fragments arranged by size: 

                1557     553     417     303     281      78
 
Bsu36I CC'TnA_GG

Cuts at:      0    2458    3189 
   Size:       2458     731
 
BtgI C'CryG_G

Cuts at:      0     104     312     353     635    1241    2624    3172    3189 
   Size:        104     208      41     282     606    1383     548      17

  Fragments arranged by size: 

                1383     606     548     282     208     104      41      17
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1059    1380    3189 
   Size:       1059     321    1809

  Fragments arranged by size: 

                1809    1059     321
 
BtsI GCAGTG_nn'

Cuts at:      0      90     376    3189 
   Size:         90     286    2813

  Fragments arranged by size: 

                2813     286      90
 
DraIII CAC_nnn'GTG

Cuts at:      0    1935    2495    3189 
   Size:       1935     560     694

  Fragments arranged by size: 

                1935     694     560
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     853    2860    3189 
   Size:        853    2007     329

  Fragments arranged by size: 

                2007     853     329
 
EaeI y'GGCC_r

Cuts at:      0     642     923    1786    1902    2065    2210    3189 
   Size:        642     281     863     116     163     145     979

  Fragments arranged by size: 

                 979     863     642     281     163     145     116
 
EagI C'GGCC_G

Cuts at:      0    1902    2065    3189 
   Size:       1902     163    1124

  Fragments arranged by size: 

                1902    1124     163
 
EarI CTCTTCn'nnn_

Cuts at:      0     758    1348    1702    2111    2266    2689    3189 
   Size:        758     590     354     409     155     423     500

  Fragments arranged by size: 

                 758     590     500     423     409     354     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     373    1248    1615    1915    3189 
   Size:        373     875     367     300    1274

  Fragments arranged by size: 

                1274     875     373     367     300
 
EcoICRI GAG'CTC

Cuts at:      0    1092    1353    1770    3189 
   Size:       1092     261     417    1419

  Fragments arranged by size: 

                1419    1092     417     261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     562     567     751    1132    1549    1558    2209 
   Size:        181     381       5     184     381     417       9     651

Cuts at:   2209    2253    3189 
   Size:         44     936

  Fragments arranged by size: 

                 936     651     417     381     381     184     181      44
                   9       5
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     802    1114    1144    2521    3189 
   Size:        802     312      30    1377     668

  Fragments arranged by size: 

                1377     802     668     312      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0      62     273     906    3081    3082    3189 
   Size:         62     211     633    2175       1     107

  Fragments arranged by size: 

                2175     633     211     107      62       1
 
EcoRI G'AATT_C

Cuts at:      0    1621    3180    3189 
   Size:       1621    1559       9

  Fragments arranged by size: 

                1621    1559       9
 
FspI TGC'GCA

Cuts at:      0     382    1972    2437    3189 
   Size:        382    1590     465     752

  Fragments arranged by size: 

                1590     752     465     382
 
FspAI rTGC'GCAy

Cuts at:      0    2437    3189 
   Size:       2437     752
 
HaeII r_GCGC'y

Cuts at:      0       7    3189 
   Size:          7    3182
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2267    2299    2366    2398    2907    2939    3189 
   Size:       2267      32      67      32     509      32     250

  Fragments arranged by size: 

                2267     509     250      67      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      70    2006    3189 
   Size:         70    1936    1183

  Fragments arranged by size: 

                1936    1183      70
 
KpnI G_GTAC'C

Cuts at:      0      25    3189 
   Size:         25    3164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1346    1934    2118    3189 
   Size:       1346     588     184    1071

  Fragments arranged by size: 

                1346    1071     588     184
 
MscI TGG'CCA

Cuts at:      0     644     925    2212    3189 
   Size:        644     281    1287     977

  Fragments arranged by size: 

                1287     977     644     281
 
MslI CAynn'nnrTG

Cuts at:      0      43     607    3189 
   Size:         43     564    2582

  Fragments arranged by size: 

                2582     564      43
 
MspA1I CmG'CkG

Cuts at:      0     726    1104    1229    1593    1830    1871    2402    2469 
   Size:        726     378     125     364     237      41     531      67

Cuts at:   2469    2610    2817    3189 
   Size:        141     207     372

  Fragments arranged by size: 

                 726     531     378     372     364     237     207     141
                 125      67      41
 
NaeI GCC'GGC

Cuts at:      0     292    1980    3189 
   Size:        292    1688    1209

  Fragments arranged by size: 

                1688    1209     292
 
NcoI C'CATG_G

Cuts at:      0     104    3189 
   Size:        104    3085
 
NdeI CA'TA_TG

Cuts at:      0     299    3189 
   Size:        299    2890
 
NgoMIV G'CCGG_C

Cuts at:      0     290    1978    3189 
   Size:        290    1688    1211

  Fragments arranged by size: 

                1688    1211     290
 
PasI CC'CwG_GG

Cuts at:      0      78    3189 
   Size:         78    3111
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     729    3189 
   Size:        729    2460
 
PfoI T'CCnGG_A

Cuts at:      0     662    1256    1526    2423    2483    3189 
   Size:        662     594     270     897      60     706

  Fragments arranged by size: 

                 897     706     662     594     270      60
 
PmlI CAC'GTG

Cuts at:      0    2054    2994    3189 
   Size:       2054     940     195

  Fragments arranged by size: 

                2054     940     195
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     565     597    3189 
   Size:        565      32    2592

  Fragments arranged by size: 

                2592     565      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     273     906    3189 
   Size:        273     633    2283

  Fragments arranged by size: 

                2283     633     273
 
PspOMI G'GGCC_C

Cuts at:      0    3081    3189 
   Size:       3081     108
 
PstI C_TGCA'G

Cuts at:      0     281     464    1337    1829    1847    2615    2735    2984 
   Size:        281     183     873     492      18     768     120     249

Cuts at:   2984    3189 
   Size:        205

  Fragments arranged by size: 

                 873     768     492     281     249     205     183     120
                  18
 
PvuII CAG'CTG

Cuts at:      0     726    1229    1830    2469    2610    2817    3189 
   Size:        726     503     601     639     141     207     372

  Fragments arranged by size: 

                 726     639     601     503     372     207     141
 
SacI G_AGCT'C

Cuts at:      0    1094    1355    1772    3189 
   Size:       1094     261     417    1417

  Fragments arranged by size: 

                1417    1094     417     261
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1348    2689    3189 
   Size:       1348    1341     500

  Fragments arranged by size: 

                1348    1341     500
 
SexAI A'CCwGG_T

Cuts at:      0     797    1931    3189 
   Size:        797    1134    1258

  Fragments arranged by size: 

                1258    1134     797
 
SfcI C'TryA_G

Cuts at:      0     277     285     460     523    1333    1656    1825    1843 
   Size:        277       8     175      63     810     323     169      18

Cuts at:   1843    2611    2731    2980    3189 
   Size:        768     120     249     209

  Fragments arranged by size: 

                 810     768     323     277     249     209     175     169
                 120      63      18       8
 
SmaI CCC'GGG

Cuts at:      0    1503    3189 
   Size:       1503    1686
 
SmlI C'TyrA_G

Cuts at:      0    1003    1360    1708    3189 
   Size:       1003     357     348    1481

  Fragments arranged by size: 

                1481    1003     357     348
 
StuI AGG'CCT

Cuts at:      0     547    2527    2737    3189 
   Size:        547    1980     210     452

  Fragments arranged by size: 

                1980     547     452     210
 
StyI C'CwwG_G

Cuts at:      0     104    1079    1145    1208    2738    3137    3189 
   Size:        104     975      66      63    1530     399      52

  Fragments arranged by size: 

                1530     975     399     104      66      63      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454     628    1819    3189 
   Size:        454     174    1191    1370

  Fragments arranged by size: 

                1370    1191     454     174
 
TatI w'GTAC_w

Cuts at:      0     599    1154    2120    2940    3189 
   Size:        599     555     966     820     249

  Fragments arranged by size: 

                 966     820     599     555     249
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     491     523    1474    1506    1705    1737    2342    2374 
   Size:        491      32     951      32     199      32     605      32

Cuts at:   2374    2479    2511    2960    2992    3189 
   Size:        105      32     449      32     197

  Fragments arranged by size: 

                 951     605     491     449     199     197     105      32
                  32      32      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1605    3189 
   Size:       1605    1584
 
XmaI C'CCGG_G

Cuts at:      0    1501    3189 
   Size:       1501    1688
 
XmnI GAAnn'nnTTC

Cuts at:      0    1280    2416    3189 
   Size:       1280    1136     773

  Fragments arranged by size: 

                1280    1136     773
 

 Enzymes that do cut:

     AarI   Acc65I    AflII     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AvaI     BanI    BanII    BbvCI    BciVI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    FspAI    HaeII    Hin4I  HindIII     KpnI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     PasI    PflMI     PfoI
     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
    SexAI     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI     BcgI     BclI    BfrBI     BglI     BmtI     BsmI    BspHI
   BssHII    BstBI  BstZ17I     ClaI    CspCI     DraI    EcoRV     FalI
     FseI   HincII     HpaI     KasI     MfeI     MluI     NarI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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