(Circular) (Six-base) MAPSORT of: pFN21ASDB0030.seq Check: 6636 from: 1 to: 9547 pFN21ASDB0030 9547 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5590 5590 Size: 9547 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4958 7357 278 Size: 53 83 186 936 3422 2399 2468 Fragments arranged by size: 3422 2468 2399 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2283 5897 5975 6314 6890 1937 Size: 346 3614 78 339 576 4594 Fragments arranged by size: 4594 3614 576 346 339 78 Acc65I G'GTAC_C Cuts at: 5431 6868 5431 Size: 1437 8110 AclI AA'CG_TT Cuts at: 1563 3874 7674 8047 1563 Size: 2311 3800 373 3063 Fragments arranged by size: 3800 3063 2311 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 3165 3366 4703 4787 5584 5762 6288 Size: 1158 375 201 1337 84 797 178 526 Cuts at: 6288 6416 7610 8994 9262 1632 Size: 128 1194 1384 268 1917 Fragments arranged by size: 1917 1384 1337 1194 1158 797 526 375 268 201 178 128 84 AflII C'TTAA_G Cuts at: 820 1017 3286 4814 820 Size: 197 2269 1528 5553 Fragments arranged by size: 5553 2269 1528 197 AflIII A'CryG_T Cuts at: 1203 4494 5458 6048 6471 8446 9423 1203 Size: 3291 964 590 423 1975 977 1327 Fragments arranged by size: 3291 1975 1327 977 964 590 423 AgeI A'CCGG_T Cuts at: 2745 3043 8378 2745 Size: 298 5335 3914 Fragments arranged by size: 5335 3914 298 AhdI GACnn_n'nnGTC Cuts at: 8276 8276 Size: 9547 AleI CACnn'nnGTG Cuts at: 1166 2513 3560 1166 Size: 1347 1047 7153 Fragments arranged by size: 7153 1347 1047 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3746 5030 5851 1184 Size: 2562 1284 821 4880 Fragments arranged by size: 4880 2562 1284 821 AlwNI CAG_nnn'CTG Cuts at: 2255 3056 3572 3716 4378 4550 5378 5848 5944 Size: 801 516 144 662 172 828 470 96 Cuts at: 5944 7290 8862 2255 Size: 1346 1572 2940 Fragments arranged by size: 2940 1572 1346 828 801 662 516 470 172 144 96 ApaI G_GGCC'C Cuts at: 2212 2644 4092 2212 Size: 432 1448 7667 Fragments arranged by size: 7667 1448 432 ApaLI G'TGCA_C Cuts at: 7604 8760 7604 Size: 1156 8391 ApoI r'AATT_y Cuts at: 1493 2453 2586 3114 3535 3783 6257 6856 7082 Size: 960 133 528 421 248 2474 599 226 Cuts at: 7082 7234 9343 1493 Size: 152 2109 1697 Fragments arranged by size: 2474 2109 1697 960 599 528 421 248 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 8101 160 Size: 872 7069 1606 Fragments arranged by size: 7069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9547 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2218 2235 3809 4704 6872 1870 Size: 73 152 123 17 1574 895 2168 4545 Fragments arranged by size: 4545 2168 1574 895 152 123 73 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2384 2417 2384 Size: 33 9514 BamHI G'GATC_C Cuts at: 2294 4000 6877 7244 2294 Size: 1706 2877 367 4597 Fragments arranged by size: 4597 2877 1706 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 3340 5014 5431 6100 Size: 325 217 627 684 869 1674 417 669 Cuts at: 6100 6868 8323 618 Size: 768 1455 1842 Fragments arranged by size: 1842 1674 1455 869 768 684 669 627 417 325 217 BanII G_rGCy'C Cuts at: 1391 2212 2422 2644 3265 4092 4470 5738 6487 Size: 821 210 222 621 827 378 1268 749 Cuts at: 6487 6866 1391 Size: 379 4072 Fragments arranged by size: 4072 1268 827 821 749 621 379 378 222 210 BbeI G_GCGC'C Cuts at: 2475 3344 2475 Size: 869 8678 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3783 4958 928 Size: 971 1884 1175 5517 Fragments arranged by size: 5517 1884 1175 971 BbvCI CC'TCA_GC Cuts at: 6021 6021 Size: 9547 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6556 6590 7738 7772 1903 Size: 34 4619 34 1148 34 3678 Fragments arranged by size: 4619 3678 1148 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6475 7439 8649 6475 Size: 964 1210 7373 Fragments arranged by size: 7373 1210 964 BclI T'GATC_A Cuts at: 1548 1548 Size: 9547 BfrBI ATG'CAT Cuts at: 4792 4792 Size: 9547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4126 5650 6629 Size: 107 122 71 631 605 2454 1524 979 Cuts at: 6629 8158 136 Size: 1529 1525 Fragments arranged by size: 2454 1529 1525 1524 979 631 605 122 107 71 BglII A'GATC_T Cuts at: 2322 3148 9542 2322 Size: 826 6394 2327 Fragments arranged by size: 6394 2327 826 BlpI GC'TnA_GC Cuts at: 6245 6753 6964 6245 Size: 508 211 8828 Fragments arranged by size: 8828 508 211 Bme1580I G_kGCm'C Cuts at: 1790 2212 2644 3222 3735 4092 4527 6105 6355 Size: 422 432 578 513 357 435 1578 250 Cuts at: 6355 7608 8764 1790 Size: 1253 1156 2573 Fragments arranged by size: 2573 1578 1253 1156 578 513 435 432 422 357 250 BmgBI CAC'GTC Cuts at: 1318 3740 3958 4987 5459 6703 1318 Size: 2422 218 1029 472 1244 4162 Fragments arranged by size: 4162 2422 1244 1029 472 218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2522 2846 2973 Size: 416 53 83 399 24 1105 324 127 Cuts at: 2973 3287 5723 7278 8236 442 Size: 314 2436 1555 958 1753 Fragments arranged by size: 2436 1753 1555 1105 958 416 399 324 314 127 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5670 1056 Size: 4614 4933 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5869 5869 Size: 9547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 2970 3062 3351 5133 5325 5526 5924 Size: 1469 262 92 289 1782 192 201 398 Cuts at: 5924 6194 6404 6611 8207 1239 Size: 270 210 207 1596 2579 Fragments arranged by size: 2579 1782 1596 1469 398 289 270 262 210 207 201 192 92 Bpu10I CC'TnA_GC Cuts at: 3399 4076 5344 6021 3399 Size: 677 1268 677 6925 Fragments arranged by size: 6925 1268 677 677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2679 3543 4859 5500 7672 8537 8835 2679 Size: 864 1316 641 2172 865 298 3391 Fragments arranged by size: 3391 2172 1316 865 864 641 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2187 3621 3752 5502 5511 6136 8210 Size: 617 688 1434 131 1750 9 625 2074 Cuts at: 8210 882 Size: 2219 Fragments arranged by size: 2219 2074 1750 1434 688 625 617 131 9 BsaAI yAC'GTr Cuts at: 493 1204 6049 6844 7338 493 Size: 711 4845 795 494 2702 Fragments arranged by size: 4845 2702 795 711 494 BsaBI GATnn'nnATC Cuts at: 4278 9541 4278 Size: 5263 4284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2472 2711 3341 4678 Size: 53 83 186 936 939 239 630 1337 Cuts at: 4678 4955 7354 7736 8371 275 Size: 277 2399 382 635 1451 Fragments arranged by size: 2399 1451 1337 939 936 635 630 382 277 239 186 83 53 BsaWI w'CCGG_w Cuts at: 2745 3043 7980 8378 8652 8799 9210 2745 Size: 298 4937 398 274 147 411 3082 Fragments arranged by size: 4937 3082 411 398 298 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 2618 2648 1178 Size: 30 165 30 918 30 267 30 8077 Fragments arranged by size: 8077 918 267 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2639 2988 3177 3323 3419 3495 3598 3878 Size: 1453 349 189 146 96 76 103 280 Cuts at: 3878 5485 5529 5630 5774 5777 6341 6380 6836 Size: 1607 44 101 144 3 564 39 456 Cuts at: 6836 6839 1186 Size: 3 3894 Fragments arranged by size: 3894 1607 1453 564 456 349 280 189 146 144 103 101 96 76 44 39 3 3 BseYI C'CCAG_C Cuts at: 2211 2269 2332 2814 3193 3394 4250 5319 5386 Size: 58 63 482 379 201 856 1069 67 Cuts at: 5386 5640 5963 8750 9246 2211 Size: 254 323 2787 496 2512 Fragments arranged by size: 2787 2512 1069 856 496 482 379 323 254 201 67 63 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 2261 2268 2429 2516 5123 5486 Size: 573 511 14 7 161 87 2607 363 Cuts at: 5486 5861 5930 1163 Size: 375 69 4780 Fragments arranged by size: 4780 2607 573 511 375 363 161 87 69 14 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3883 6997 7758 8786 1540 Size: 275 186 1882 3114 761 1028 2301 Fragments arranged by size: 3114 2301 1882 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5094 7608 7693 8764 5094 Size: 2514 85 1071 5877 Fragments arranged by size: 5877 2514 1071 85 BsmI GAATG_Cn' Cuts at: 6287 7058 7151 6287 Size: 771 93 8683 Fragments arranged by size: 8683 771 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2702 3580 2702 Size: 878 8669 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2212 2422 2644 3222 3265 3735 4092 Size: 399 422 210 222 578 43 470 357 Cuts at: 4092 4470 4527 5094 5738 6105 6355 6487 6866 Size: 378 57 567 644 367 250 132 379 Cuts at: 6866 7608 7693 8764 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 644 578 567 470 422 399 379 378 367 357 250 222 210 132 85 57 43 BspEI T'CCGG_A Cuts at: 9210 9210 Size: 9547 BspHI T'CATG_A Cuts at: 3118 6489 7436 9166 3118 Size: 3371 947 1730 3499 Fragments arranged by size: 3499 3371 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5590 6672 6902 844 Size: 4746 1082 230 3489 Fragments arranged by size: 4746 3489 1082 230 BsrBI CCG'CTC Cuts at: 1394 1823 5530 5620 7434 1394 Size: 429 3707 90 1814 3507 Fragments arranged by size: 3707 3507 1814 429 90 BsrDI GCAATG_nn' Cuts at: 65 1248 2763 3222 4188 5916 8042 8216 65 Size: 1183 1515 459 966 1728 2126 174 1396 Fragments arranged by size: 2126 1728 1515 1396 1183 966 459 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 2745 2772 3043 4129 5559 8191 Size: 110 300 635 27 271 1086 1430 2632 Cuts at: 8191 8378 1700 Size: 187 2869 Fragments arranged by size: 2869 2632 1430 1086 635 300 271 187 110 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9547 BssSI C'ACGA_G Cuts at: 5922 7607 8619 5922 Size: 1685 1012 6850 Fragments arranged by size: 6850 1685 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2255 2765 4987 5944 5953 794 Size: 1461 510 2222 957 9 4388 Fragments arranged by size: 4388 2222 1461 957 510 9 BstBI TT'CG_AA Cuts at: 2007 8351 2007 Size: 6344 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9547 BstXI CCAn_nnnn'nTGG Cuts at: 2515 3094 3562 6281 2515 Size: 579 468 2719 5781 Fragments arranged by size: 5781 2719 579 468 BstYI r'GATC_y Cuts at: 1972 2294 2322 2593 3148 4000 4993 5055 6177 Size: 322 28 271 555 852 993 62 1122 Cuts at: 6177 6877 7244 7629 7646 9098 9184 9196 9542 Size: 700 367 385 17 1452 86 12 346 Cuts at: 9542 1972 Size: 1977 Fragments arranged by size: 1977 1452 1122 993 852 700 555 385 367 346 322 271 86 62 28 17 12 BstZ17I GTA'TAC Cuts at: 6315 6315 Size: 9547 Bsu36I CC'TnA_GG Cuts at: 3243 3744 5646 3243 Size: 501 1902 7144 Fragments arranged by size: 7144 1902 501 BtgI C'CryG_G Cuts at: 513 1065 4084 4123 5167 5551 5689 6105 513 Size: 552 3019 39 1044 384 138 416 3955 Fragments arranged by size: 3955 3019 1044 552 416 384 138 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8808 Fragments arranged by size: 8808 683 56 BtsI GCAGTG_nn' Cuts at: 2230 2646 3730 3986 5131 5917 6555 7063 7857 Size: 416 1084 256 1145 786 638 508 794 Cuts at: 7857 7877 2230 Size: 20 3900 Fragments arranged by size: 3900 1145 1084 794 786 638 508 416 256 20 ClaI AT'CG_AT Cuts at: 1554 4579 5277 1554 Size: 3025 698 5824 Fragments arranged by size: 5824 3025 698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2365 2400 529 Size: 35 1801 35 7676 Fragments arranged by size: 7676 1801 35 35 DraI TTT'AAA Cuts at: 6851 6851 Size: 9547 DraIII CAC_nnn'GTG Cuts at: 3109 3109 Size: 9547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4802 8554 809 Size: 507 3486 3752 1802 Fragments arranged by size: 3752 3486 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2735 2774 3992 Size: 54 1425 50 275 18 905 39 1218 Cuts at: 3992 4063 4401 5115 6343 6994 7883 9512 8 Size: 71 338 714 1228 651 889 1629 43 Fragments arranged by size: 1629 1425 1228 1218 905 889 714 651 338 275 71 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 6994 1537 Size: 275 5182 4090 Fragments arranged by size: 5182 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3423 4683 4692 4824 5873 7477 1353 Size: 2070 1260 9 132 1049 1604 3423 Fragments arranged by size: 3423 2070 1604 1260 1049 132 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8134 8508 8654 387 Size: 1064 138 6545 374 146 1280 Fragments arranged by size: 6545 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2708 2790 2970 3062 3165 3351 3366 Size: 393 1076 82 180 92 103 186 15 Cuts at: 3366 4703 4787 5133 5325 5526 5584 5762 5924 Size: 1337 84 346 192 201 58 178 162 Cuts at: 5924 6194 6288 6404 6416 6611 7610 8207 8994 Size: 270 94 116 12 195 999 597 787 Cuts at: 8994 9262 1239 Size: 268 1524 Fragments arranged by size: 1524 1337 1076 999 787 597 393 346 270 268 201 195 192 186 180 178 162 116 103 94 92 84 82 58 15 12 EcoNI CCTnn'n_nnAGG Cuts at: 3690 5675 6323 3690 Size: 1985 648 6914 Fragments arranged by size: 6914 1985 648 EcoO109I rG'GnC_Cy Cuts at: 2048 2346 2460 2640 4089 4142 4163 4318 5036 Size: 298 114 180 1449 53 21 155 718 Cuts at: 5036 5153 6206 6631 6745 2048 Size: 117 1053 425 114 4850 Fragments arranged by size: 4850 1449 1053 718 425 298 180 155 117 114 114 53 21 EcoRI G'AATT_C Cuts at: 2586 6856 7234 2586 Size: 4270 378 4899 Fragments arranged by size: 4899 4270 378 EcoRV GAT'ATC Cuts at: 2617 2617 Size: 9547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3050 4853 5614 6260 3050 Size: 1803 761 646 6337 Fragments arranged by size: 6337 1803 761 646 FspI TGC'GCA Cuts at: 5657 8053 5657 Size: 2396 7151 HaeII r_GCGC'y Cuts at: 1678 2111 2475 3344 4153 4517 4726 6617 8694 Size: 433 364 869 809 364 209 1891 2077 Cuts at: 8694 1678 Size: 2531 Fragments arranged by size: 2531 2077 1891 869 809 433 364 364 209 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 2732 2764 3152 Size: 32 135 32 1046 32 82 32 388 Cuts at: 3152 3184 4817 4849 5079 5111 5354 5386 5762 Size: 32 1633 32 230 32 243 32 376 Cuts at: 5762 5794 6812 6844 8188 8220 8262 8294 1373 Size: 32 1018 32 1344 32 42 32 2626 Fragments arranged by size: 2626 1633 1344 1046 1018 388 376 243 230 135 82 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4776 6891 7137 669 Size: 988 281 2838 2115 246 3079 Fragments arranged by size: 3079 2838 2115 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9547 HpaI GTT'AAC Cuts at: 4776 7137 4776 Size: 2361 7186 KasI G'GCGC_C Cuts at: 2471 3340 2471 Size: 869 8678 KpnI G_GTAC'C Cuts at: 5435 6872 5435 Size: 1437 8110 MfeI C'AATT_G Cuts at: 5008 7146 5008 Size: 2138 7409 MluI A'CGCG_T Cuts at: 9423 9423 Size: 9547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2858 3254 3604 6312 6492 8661 8845 1305 Size: 1553 396 350 2708 180 2169 184 2007 Fragments arranged by size: 2708 2169 2007 1553 396 350 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 4403 5117 6345 10 Size: 54 1425 343 2571 714 1228 3212 Fragments arranged by size: 3212 2571 1425 1228 714 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3560 3951 4797 5099 5811 7505 Size: 648 1347 1047 391 846 302 712 1694 Cuts at: 7505 7864 8023 518 Size: 359 159 2042 Fragments arranged by size: 2042 1694 1347 1047 846 712 648 391 359 302 159 MspA1I CmG'CkG Cuts at: 1606 2273 3278 4378 4399 4503 5337 5941 5953 Size: 667 1005 1100 21 104 834 604 12 Cuts at: 5953 6382 6418 6752 6963 7640 8788 9033 1606 Size: 429 36 334 211 677 1148 245 2120 Fragments arranged by size: 2120 1148 1100 1005 834 677 667 604 429 334 245 211 104 36 21 12 NaeI GCC'GGC Cuts at: 4131 4131 Size: 9547 NarI GG'CG_CC Cuts at: 2472 3341 2472 Size: 869 8678 NcoI C'CATG_G Cuts at: 513 1065 6105 513 Size: 552 5040 3955 Fragments arranged by size: 5040 3955 552 NdeI CA'TA_TG Cuts at: 387 5931 387 Size: 5544 4003 NgoMIV G'CCGG_C Cuts at: 4129 4129 Size: 9547 NheI G'CTAG_C Cuts at: 1052 5666 1052 Size: 4614 4933 NotI GC'GGCC_GC Cuts at: 6994 6994 Size: 9547 NsiI A_TGCA'T Cuts at: 4794 4794 Size: 9547 NspI r_CATG'y Cuts at: 1132 1229 4498 6905 8450 1132 Size: 97 3269 2407 1545 2229 Fragments arranged by size: 3269 2407 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2341 6319 1348 Size: 993 3978 4576 Fragments arranged by size: 4576 3978 993 PciI A'CATG_T Cuts at: 4494 8446 4494 Size: 3952 5595 PflMI CCAn_nnn'nTGG Cuts at: 1108 4100 1108 Size: 2992 6555 PmeI GTTT'AAAC Cuts at: 6851 6851 Size: 9547 PmlI CAC'GTG Cuts at: 6049 6049 Size: 9547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7616 7648 9129 9161 7616 Size: 32 1481 32 8002 Fragments arranged by size: 8002 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2460 4142 4163 4318 5036 6206 6745 2460 Size: 1682 21 155 718 1170 539 5262 Fragments arranged by size: 5262 1682 1170 718 539 155 21 PshAI GACnn'nnGTC Cuts at: 1613 5133 6497 1613 Size: 3520 1364 4663 Fragments arranged by size: 4663 3520 1364 PsiI TTA'TAA Cuts at: 7117 7117 Size: 9547 PspOMI G'GGCC_C Cuts at: 2208 2640 4088 2208 Size: 432 1448 7667 Fragments arranged by size: 7667 1448 432 PstI C_TGCA'G Cuts at: 830 2190 3694 4983 6090 6579 6899 830 Size: 1360 1504 1289 1107 489 320 3478 Fragments arranged by size: 3478 1504 1360 1289 1107 489 320 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9547 PvuII CAG'CTG Cuts at: 2273 3278 4378 4399 5941 5953 6382 2273 Size: 1005 1100 21 1542 12 429 5438 Fragments arranged by size: 5438 1542 1100 1005 429 21 12 SalI G'TCGA_C Cuts at: 1936 6889 1936 Size: 4953 4594 SanDI GG'GwC_CC Cuts at: 4142 4163 5036 4142 Size: 21 873 8653 Fragments arranged by size: 8653 873 21 SapI GCTCTTCn'nnn_ Cuts at: 4683 5873 4683 Size: 1190 8357 SbfI CC_TGCA'GG Cuts at: 3694 6899 3694 Size: 3205 6342 ScaI AGT'ACT Cuts at: 5398 5987 7795 9507 5398 Size: 589 1808 1712 5438 Fragments arranged by size: 5438 1808 1712 589 SfcI C'TryA_G Cuts at: 826 1045 2186 2448 2931 3690 4979 6086 6575 Size: 219 1141 262 483 759 1289 1107 489 Cuts at: 6575 6608 6724 6895 8030 8711 8902 826 Size: 33 116 171 1135 681 191 1471 Fragments arranged by size: 1471 1289 1141 1135 1107 759 681 489 483 262 219 191 171 116 33 SfoI GGC'GCC Cuts at: 2473 3342 2473 Size: 869 8678 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9547 SgrAI Cr'CCGG_yG Cuts at: 1700 2110 1700 Size: 410 9137 SmaI CCC'GGG Cuts at: 1872 2097 3811 6874 1872 Size: 225 1714 3063 4545 Fragments arranged by size: 4545 3063 1714 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2694 3286 3558 4814 4838 5479 Size: 197 926 751 592 272 1256 24 641 Cuts at: 5479 7651 8552 8814 820 Size: 2172 901 262 1553 Fragments arranged by size: 2172 1553 1256 926 901 751 641 592 272 262 197 24 SnaBI TAC'GTA Cuts at: 493 6844 7338 493 Size: 6351 494 2702 Fragments arranged by size: 6351 2702 494 SpeI A'CTAG_T Cuts at: 152 5393 152 Size: 5241 4306 SphI G_CATG'C Cuts at: 1132 6905 1132 Size: 5773 3774 SspI AAT'ATT Cuts at: 5 52 7471 5 Size: 47 7419 2081 Fragments arranged by size: 7419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9547 StyI C'CwwG_G Cuts at: 513 1065 2488 2602 3318 4060 4094 5118 5781 Size: 552 1423 114 716 742 34 1024 663 Cuts at: 5781 5992 6105 6986 513 Size: 211 113 881 3074 Fragments arranged by size: 3074 1423 1024 881 742 716 663 552 211 114 113 34 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2674 4223 5002 5526 6094 7574 7591 Size: 1255 1094 1549 779 524 568 1480 17 Cuts at: 7591 7744 7929 8374 325 Size: 153 185 445 1498 Fragments arranged by size: 1549 1498 1480 1255 1094 779 568 524 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5396 5985 7793 Size: 274 80 33 51 1443 3419 589 1808 Cuts at: 7793 9505 96 Size: 1712 138 Fragments arranged by size: 3419 1808 1712 1443 589 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9507 9539 9507 Size: 32 9515 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9547 XbaI T'CTAG_A Cuts at: 6883 6883 Size: 9547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4477 4903 4477 Size: 426 9121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9547 XmaI C'CCGG_G Cuts at: 1870 2095 3809 6872 1870 Size: 225 1714 3063 4545 Fragments arranged by size: 4545 3063 1714 225 XmnI GAAnn'nnTTC Cuts at: 5408 7676 5408 Size: 2268 7279 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4956 7355 276 Size: 53 83 186 936 3422 2399 2468 Fragments arranged by size: 3422 2468 2399 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BsiWI EcoICRI FseI FspAI NruI PacI PfoI PspXI PsrI RsrII SacI SacII SexAI SfiI SrfI SwaI