(Circular) (Six-base) MAPSORT of: pFN21ASDB0012.seq Check: 7195 from: 1 to: 6160 pFN21ASDB0012 6160 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2045 2045 Size: 6160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2715 3275 3413 4223 5607 5875 1632 Size: 1083 560 138 810 1384 268 1917 Fragments arranged by size: 1917 1384 1083 810 560 268 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2474 2981 3903 5475 2474 Size: 507 922 1572 3159 Fragments arranged by size: 3159 1572 922 507 ApaI G_GGCC'C Cuts at: 2086 2086 Size: 6160 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 3047 3469 3695 3847 5956 1493 Size: 1554 422 226 152 2109 1697 Fragments arranged by size: 2109 1697 1554 422 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 2539 3485 1870 Size: 73 596 946 4545 Fragments arranged by size: 4545 946 596 73 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2103 2461 2762 3185 3481 Size: 325 217 627 316 358 301 423 296 Cuts at: 3481 4936 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 423 358 325 316 301 296 217 BanII G_rGCy'C Cuts at: 1391 2086 2216 3307 3479 1391 Size: 695 130 1091 172 4072 Fragments arranged by size: 4072 1091 695 172 130 BbeI G_GCGC'C Cuts at: 2465 2465 Size: 6160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3401 928 Size: 971 1502 3687 Fragments arranged by size: 3687 1502 971 BbvCI CC'TCA_GC Cuts at: 2742 3117 2742 Size: 375 5785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2683 4052 5262 2683 Size: 1369 1210 3581 Fragments arranged by size: 3581 1369 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 2321 2432 Size: 107 122 71 631 605 466 183 111 Cuts at: 2432 4771 136 Size: 2339 1525 Fragments arranged by size: 2339 1525 631 605 466 183 122 111 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2086 4221 5377 1790 Size: 296 2135 1156 2573 Fragments arranged by size: 2573 2135 1156 296 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2138 2384 3891 Size: 416 53 83 399 24 721 246 1507 Cuts at: 3891 4849 442 Size: 958 1753 Fragments arranged by size: 1753 1507 958 721 416 399 246 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3145 3145 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2546 4820 1239 Size: 1085 222 2274 2579 Fragments arranged by size: 2579 2274 1085 222 Bpu10I CC'TnA_GC Cuts at: 2742 3117 2742 Size: 375 5785 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 4285 5150 5448 2264 Size: 2021 865 298 2976 Fragments arranged by size: 2976 2021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2529 4823 882 Size: 617 1030 2294 2219 Fragments arranged by size: 2294 2219 1030 617 BsaAI yAC'GTr Cuts at: 493 1204 3457 3951 493 Size: 711 2253 494 2702 Fragments arranged by size: 2702 2253 711 494 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2290 2462 3967 4349 Size: 53 83 186 936 757 172 1505 382 Cuts at: 4349 4984 275 Size: 635 1451 Fragments arranged by size: 1505 1451 936 757 635 382 186 172 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2544 2574 2901 2931 1178 Size: 30 165 30 1141 30 327 30 4407 Fragments arranged by size: 4407 1141 327 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2213 2216 2621 2633 2636 2744 3134 1186 Size: 1027 3 405 12 3 108 390 4212 Fragments arranged by size: 4212 1027 405 390 108 12 3 3 BseYI C'CCAG_C Cuts at: 2436 2467 5363 5859 2436 Size: 31 2896 496 2737 Fragments arranged by size: 2896 2737 496 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2212 2339 1163 Size: 573 476 127 4984 Fragments arranged by size: 4984 573 476 127 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2533 3610 4371 5399 1540 Size: 275 186 532 1077 761 1028 2301 Fragments arranged by size: 2301 1077 1028 761 532 275 186 BsiHKAI G_wGCw'C Cuts at: 4221 4306 5377 4221 Size: 85 1071 5004 Fragments arranged by size: 5004 1071 85 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 BsmBI CGTCTCn'nnnn_ Cuts at: 2637 2637 Size: 6160 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2216 3307 3479 4221 4306 5377 Size: 399 296 130 1091 172 742 85 1071 Cuts at: 5377 1391 Size: 2174 Fragments arranged by size: 2174 1091 1071 742 399 296 172 130 85 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2045 2124 3515 844 Size: 1201 79 1391 3489 Fragments arranged by size: 3489 1391 1201 79 BsrBI CCG'CTC Cuts at: 1394 1823 3332 4047 1394 Size: 429 1509 715 3507 Fragments arranged by size: 3507 1509 715 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2706 4655 4829 65 Size: 1183 1458 1949 174 1396 Fragments arranged by size: 1949 1458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4804 4991 1700 Size: 110 2994 187 2869 Fragments arranged by size: 2994 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2983 96 Size: 2887 3273 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 2046 2235 2934 794 Size: 1252 189 699 4020 Fragments arranged by size: 4020 1252 699 189 BstBI TT'CG_AA Cuts at: 2007 4964 2007 Size: 2957 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstYI r'GATC_y Cuts at: 1972 3490 3857 4242 4259 5711 5797 5809 6155 Size: 1518 367 385 17 1452 86 12 346 Cuts at: 6155 1972 Size: 1977 Fragments arranged by size: 1977 1518 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2502 2518 3162 3276 513 Size: 552 957 480 16 644 114 3397 Fragments arranged by size: 3397 957 644 552 480 114 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3048 3185 487 Size: 683 56 1822 137 3462 Fragments arranged by size: 3462 1822 683 137 56 BtsI GCAGTG_nn' Cuts at: 2331 3676 4470 4490 2331 Size: 1345 794 20 4001 Fragments arranged by size: 4001 1345 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3231 3266 529 Size: 35 2667 35 3423 Fragments arranged by size: 3423 2667 35 35 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2130 2217 2412 Size: 54 1425 50 275 18 300 87 195 Cuts at: 2412 2499 2515 3607 4496 6125 8 Size: 87 16 1092 889 1629 43 Fragments arranged by size: 1629 1425 1092 889 300 275 195 87 87 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2730 3026 4090 1353 Size: 1269 108 296 1064 3423 Fragments arranged by size: 3423 1269 1064 296 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2137 4747 5121 5267 387 Size: 1064 138 548 2610 374 146 1280 Fragments arranged by size: 2610 1280 1064 548 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2546 2715 3275 3413 4223 4820 Size: 393 692 222 169 560 138 810 597 Cuts at: 4820 5607 5875 1239 Size: 787 268 1524 Fragments arranged by size: 1524 810 787 692 597 560 393 268 222 169 138 EcoO109I rG'GnC_Cy Cuts at: 2523 2632 3103 2523 Size: 109 471 5580 Fragments arranged by size: 5580 471 109 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 6160 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2465 2474 5307 1678 Size: 787 9 2833 2531 Fragments arranged by size: 2833 2531 787 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3064 3096 4801 4833 4875 Size: 32 135 32 1492 32 1705 32 42 Cuts at: 4875 4907 1373 Size: 32 2626 Fragments arranged by size: 2626 1705 1492 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6160 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 2461 2461 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 10 Size: 54 1425 343 300 4038 Fragments arranged by size: 4038 1425 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2997 4118 4477 4636 518 Size: 648 1831 1121 359 159 2042 Fragments arranged by size: 2042 1831 1121 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2185 3576 4253 5401 5646 1606 Size: 474 105 1391 677 1148 245 2120 Fragments arranged by size: 2120 1391 1148 677 474 245 105 NarI GG'CG_CC Cuts at: 2462 2462 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2022 3162 513 Size: 552 957 1140 3511 Fragments arranged by size: 3511 1140 957 552 NdeI CA'TA_TG Cuts at: 387 2994 387 Size: 2607 3553 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 2077 3358 3518 5063 1132 Size: 97 848 1281 160 1545 2229 Fragments arranged by size: 2229 1545 1281 848 160 97 PasI CC'CwG_GG Cuts at: 1348 3307 1348 Size: 1959 4201 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 2759 1108 Size: 1651 4509 PfoI T'CCnGG_A Cuts at: 2163 2163 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2523 2632 3103 2523 Size: 109 471 5580 Fragments arranged by size: 5580 471 109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PspOMI G'GGCC_C Cuts at: 2082 2082 Size: 6160 PstI C_TGCA'G Cuts at: 830 3512 830 Size: 2682 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SanDI GG'GwC_CC Cuts at: 2523 2523 Size: 6160 SapI GCTCTTCn'nnn_ Cuts at: 3026 3026 Size: 6160 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 3288 4408 6120 3288 Size: 1120 1712 3328 Fragments arranged by size: 3328 1712 1120 SfcI C'TryA_G Cuts at: 826 1045 3508 4643 5324 5515 826 Size: 219 2463 1135 681 191 1471 Fragments arranged by size: 2463 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2138 2432 2138 Size: 294 5866 SfoI GGC'GCC Cuts at: 2463 2463 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 4264 5165 5427 820 Size: 197 926 336 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 3457 3951 493 Size: 2964 494 2702 Fragments arranged by size: 2964 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6160 StyI C'CwwG_G Cuts at: 513 1065 2022 2758 3162 3599 513 Size: 552 957 736 404 437 3074 Fragments arranged by size: 3074 957 736 552 437 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2547 4187 4204 4357 4542 4987 325 Size: 1255 967 1640 17 153 185 445 1498 Fragments arranged by size: 1640 1498 1255 967 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2983 3286 3297 Size: 274 80 33 51 1443 1006 303 11 Cuts at: 3297 4406 6118 96 Size: 1109 1712 138 Fragments arranged by size: 1712 1443 1109 1006 303 274 138 80 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 2364 2396 2544 2576 6120 6152 2267 Size: 32 65 32 148 32 3544 32 2275 Fragments arranged by size: 3544 2275 148 65 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2494 Size: 6160 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 2963 3171 4289 2963 Size: 208 1118 4834 Fragments arranged by size: 4834 1118 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SexAI SrfI SwaI