Restriction map for pFN21ASDB0012
 (Linear) (Six-base) MAPSORT of: KIBB0012.seq  Check: 3382  from: 1  to: 1431



KIBB0012.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      22    1431 
   Size:         22    1409
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     692    1252    1390    1431 
   Size:        692     560     138      41

  Fragments arranged by size: 

                 692     560     138      41
 
AlwNI CAG_nnn'CTG

Cuts at:      0     451     958    1431 
   Size:        451     507     473

  Fragments arranged by size: 

                 507     473     451
 
ApaI G_GGCC'C

Cuts at:      0      63    1431 
   Size:         63    1368
 
ApoI r'AATT_y

Cuts at:      0    1024    1431 
   Size:       1024     407
 
AvaI C'yCGr_G

Cuts at:      0     516    1431 
   Size:        516     915
 
BanI G'GyrC_C

Cuts at:      0      80     438     739    1162    1431 
   Size:         80     358     301     423     269

  Fragments arranged by size: 

                 423     358     301     269      80
 
BanII G_rGCy'C

Cuts at:      0      63     193    1284    1431 
   Size:         63     130    1091     147

  Fragments arranged by size: 

                1091     147     130      63
 
BbeI G_GCGC'C

Cuts at:      0     442    1431 
   Size:        442     989
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1378    1431 
   Size:       1378      53
 
BbvCI CC'TCA_GC

Cuts at:      0     719    1094    1431 
   Size:        719     375     337

  Fragments arranged by size: 

                 719     375     337
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     660    1431 
   Size:        660     771
 
BglI GCCn_nnn'nGGC

Cuts at:      0     115     298     409    1431 
   Size:        115     183     111    1022

  Fragments arranged by size: 

                1022     183     115     111
 
Bme1580I G_kGCm'C

Cuts at:      0      63    1431 
   Size:         63    1368
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     115     361    1431 
   Size:        115     246    1070

  Fragments arranged by size: 

                1070     246     115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1122    1431 
   Size:       1122     309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     523    1431 
   Size:        301     222     908

  Fragments arranged by size: 

                 908     301     222
 
Bpu10I CC'TnA_GC

Cuts at:      0     719    1094    1431 
   Size:        719     375     337

  Fragments arranged by size: 

                 719     375     337
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241    1431 
   Size:        241    1190
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     506    1431 
   Size:        506     925
 
BsaHI Gr'CG_yC

Cuts at:      0     267     439    1431 
   Size:        267     172     992

  Fragments arranged by size: 

                 992     267     172
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     521     551     878     908    1431 
   Size:        521      30     327      30     523

  Fragments arranged by size: 

                 523     521     327      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     190     193     598     610     613     721    1111    1431 
   Size:        190       3     405      12       3     108     390     320

  Fragments arranged by size: 

                 405     390     320     190     108      12       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     413     444    1431 
   Size:        413      31     987

  Fragments arranged by size: 

                 987     413      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     316    1431 
   Size:        189     127    1115

  Fragments arranged by size: 

                1115     189     127
 
BsiEI CG_ry'CG

Cuts at:      0     510    1431 
   Size:        510     921
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     614    1431 
   Size:        614     817
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     193    1284    1431 
   Size:         63     130    1091     147

  Fragments arranged by size: 

                1091     147     130      63
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      22     101    1431 
   Size:         22      79    1330

  Fragments arranged by size: 

                1330      79      22
 
BsrBI CCG'CTC

Cuts at:      0    1309    1431 
   Size:       1309     122
 
BsrDI GCAATG_nn'

Cuts at:      0     683    1431 
   Size:        683     748
 
BsrGI T'GTAC_A

Cuts at:      0     960    1431 
   Size:        960     471
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      23     212     911    1431 
   Size:         23     189     699     520

  Fragments arranged by size: 

                 699     520     189      23
 
BtgI C'CryG_G

Cuts at:      0     479     495    1139    1253    1431 
   Size:        479      16     644     114     178

  Fragments arranged by size: 

                 644     479     178     114      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1025    1162    1431 
   Size:       1025     137     269

  Fragments arranged by size: 

                1025     269     137
 
BtsI GCAGTG_nn'

Cuts at:      0     308    1431 
   Size:        308    1123
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1208    1243    1431 
   Size:       1208      35     188

  Fragments arranged by size: 

                1208     188      35
 
EaeI y'GGCC_r

Cuts at:      0     107     194     389     476     492    1431 
   Size:        107      87     195      87      16     939

  Fragments arranged by size: 

                 939     195     107      87      87      16
 
EarI CTCTTCn'nnn_

Cuts at:      0     599     707    1003    1431 
   Size:        599     108     296     428

  Fragments arranged by size: 

                 599     428     296     108
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     114    1431 
   Size:        114    1317
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     523     692    1252    1390    1431 
   Size:        301     222     169     560     138      41

  Fragments arranged by size: 

                 560     301     222     169     138      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     500     609    1080    1431 
   Size:        500     109     471     351

  Fragments arranged by size: 

                 500     471     351     109
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     930    1431 
   Size:        930     501
 
HaeII r_GCGC'y

Cuts at:      0     442     451    1431 
   Size:        442       9     980

  Fragments arranged by size: 

                 980     442       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1041    1073    1431 
   Size:       1041      32     358

  Fragments arranged by size: 

                1041     358      32
 
KasI G'GCGC_C

Cuts at:      0     438    1431 
   Size:        438     993
 
MscI TGG'CCA

Cuts at:      0     109    1431 
   Size:        109    1322
 
MslI CAynn'nnrTG

Cuts at:      0     974    1431 
   Size:        974     457
 
MspA1I CmG'CkG

Cuts at:      0      57     162    1431 
   Size:         57     105    1269

  Fragments arranged by size: 

                1269     105      57
 
NarI GG'CG_CC

Cuts at:      0     439    1431 
   Size:        439     992
 
NcoI C'CATG_G

Cuts at:      0    1139    1431 
   Size:       1139     292
 
NdeI CA'TA_TG

Cuts at:      0     971    1431 
   Size:        971     460
 
NspI r_CATG'y

Cuts at:      0      54    1335    1431 
   Size:         54    1281      96

  Fragments arranged by size: 

                1281      96      54
 
PasI CC'CwG_GG

Cuts at:      0    1284    1431 
   Size:       1284     147
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     736    1431 
   Size:        736     695
 
PfoI T'CCnGG_A

Cuts at:      0     140    1431 
   Size:        140    1291
 
PpuMI rG'GwC_Cy

Cuts at:      0     500     609    1080    1431 
   Size:        500     109     471     351

  Fragments arranged by size: 

                 500     471     351     109
 
PspOMI G'GGCC_C

Cuts at:      0      59    1431 
   Size:         59    1372
 
SanDI GG'GwC_CC

Cuts at:      0     500    1431 
   Size:        500     931
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1003    1431 
   Size:       1003     428
 
ScaI AGT'ACT

Cuts at:      0    1265    1431 
   Size:       1265     166
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     115     409    1431 
   Size:        115     294    1022

  Fragments arranged by size: 

                1022     294     115
 
SfoI GGC'GCC

Cuts at:      0     440    1431 
   Size:        440     991
 
SmlI C'TyrA_G

Cuts at:      0     256    1431 
   Size:        256    1175
 
StyI C'CwwG_G

Cuts at:      0     735    1139    1431 
   Size:        735     404     292

  Fragments arranged by size: 

                 735     404     292
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     524    1431 
   Size:        524     907
 
TatI w'GTAC_w

Cuts at:      0     960    1263    1274    1431 
   Size:        960     303      11     157

  Fragments arranged by size: 

                 960     303     157      11
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     244     276     341     373     521     553    1431 
   Size:        244      32      65      32     148      32     878

  Fragments arranged by size: 

                 878     244     148      65      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    1431 
   Size:        471     960
 
XmnI GAAnn'nnTTC

Cuts at:      0     940    1148    1431 
   Size:        940     208     283

  Fragments arranged by size: 

                 940     283     208
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BglI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI
   BstAPI     BtgI    BtgZI     BtsI    CspCI     EaeI     EarI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI    SanDI     SapI     ScaI     SfiI     SfoI     SmlI
     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BcgI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI    BsaAI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI
    BspEI    BspHI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SbfI    SexAI     SfcI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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