(Circular) (Six-base) MAPSORT of: pFN21ASDA1852.seq Check: 8729 from: 1 to: 7105 pFN21ASDA1852 7105 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2322 2322 Size: 7105 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3245 4915 278 Size: 53 83 186 936 1709 1670 2468 Fragments arranged by size: 2468 1709 1670 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4448 1937 Size: 2511 4594 Acc65I G'GTAC_C Cuts at: 4426 4426 Size: 7105 AclI AA'CG_TT Cuts at: 1563 5232 5605 1563 Size: 3669 373 3063 Fragments arranged by size: 3669 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3400 3736 3988 5168 6552 6820 1632 Size: 1768 336 252 1180 1384 268 1917 Fragments arranged by size: 1917 1768 1384 1180 336 268 252 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6908 AflIII A'CryG_T Cuts at: 1203 6004 6981 1203 Size: 4801 977 1327 Fragments arranged by size: 4801 1327 977 AgeI A'CCGG_T Cuts at: 5936 5936 Size: 7105 AhdI GACnn_n'nnGTC Cuts at: 2307 5834 2307 Size: 3527 3578 AleI CACnn'nnGTG Cuts at: 1166 4047 1166 Size: 2881 4224 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7105 AlwNI CAG_nnn'CTG Cuts at: 4848 6420 4848 Size: 1572 5533 ApaLI G'TGCA_C Cuts at: 5162 6318 5162 Size: 1156 5949 ApoI r'AATT_y Cuts at: 1493 2654 2949 3424 3450 4038 4264 4414 4640 Size: 1161 295 475 26 588 226 150 226 Cuts at: 4640 4792 6901 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1161 588 475 295 226 226 152 150 26 AseI AT'TA_AT Cuts at: 160 1032 5659 160 Size: 872 4627 1606 Fragments arranged by size: 4627 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 AvaI C'yCGr_G Cuts at: 1870 1943 4430 1870 Size: 73 2487 4545 Fragments arranged by size: 4545 2487 73 BamHI G'GATC_C Cuts at: 4435 4802 4435 Size: 367 6738 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2054 4426 5881 618 Size: 325 217 627 267 2372 1455 1842 Fragments arranged by size: 2372 1842 1455 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2113 2181 3011 3260 3596 3764 4424 1391 Size: 722 68 830 249 336 168 660 4072 Fragments arranged by size: 4072 830 722 660 336 249 168 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2608 928 Size: 971 709 5425 Fragments arranged by size: 5425 971 709 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5296 5330 1903 Size: 34 3359 34 3678 Fragments arranged by size: 3678 3359 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2136 4997 6207 2136 Size: 2861 1210 3034 Fragments arranged by size: 3034 2861 1210 BclI T'GATC_A Cuts at: 1548 2938 1548 Size: 1390 5715 BfrBI ATG'CAT Cuts at: 3622 4294 3622 Size: 672 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 5716 136 Size: 107 122 71 631 605 436 3608 1525 Fragments arranged by size: 3608 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 7100 7100 Size: 7105 BlpI GC'TnA_GC Cuts at: 3343 3847 4522 3343 Size: 504 675 5926 Fragments arranged by size: 5926 675 504 Bme1580I G_kGCm'C Cuts at: 1790 2059 5166 6322 1790 Size: 269 3107 1156 2573 Fragments arranged by size: 3107 2573 1156 269 BmgBI CAC'GTC Cuts at: 1318 2221 1318 Size: 903 6202 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2184 4836 5794 Size: 416 53 83 399 24 767 2652 958 Cuts at: 5794 442 Size: 1753 Fragments arranged by size: 2652 1753 958 767 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 2733 2984 2371 Size: 362 251 6492 Fragments arranged by size: 6492 362 251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2318 2521 2895 3274 3312 3610 3648 Size: 935 144 203 374 379 38 298 38 Cuts at: 3648 3732 3778 3984 4152 4236 5765 1239 Size: 84 46 206 168 84 1529 2579 Fragments arranged by size: 2579 1529 935 379 374 298 206 203 168 144 84 84 46 38 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5230 6095 6393 5230 Size: 865 298 5942 Fragments arranged by size: 5942 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 5768 882 Size: 617 795 3474 2219 Fragments arranged by size: 3474 2219 795 617 BsaAI yAC'GTr Cuts at: 493 1204 2140 4402 4896 493 Size: 711 936 2262 494 2702 Fragments arranged by size: 2702 2262 936 711 494 BsaBI GATnn'nnATC Cuts at: 7099 7099 Size: 7105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3242 4912 5294 5929 Size: 53 83 186 936 1709 1670 382 635 Cuts at: 5929 275 Size: 1451 Fragments arranged by size: 1709 1670 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 5538 5936 6210 6357 6768 4116 Size: 1422 398 274 147 411 4453 Fragments arranged by size: 4453 1422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6880 Fragments arranged by size: 6880 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2179 1186 Size: 985 8 6112 Fragments arranged by size: 6112 985 8 BseYI C'CCAG_C Cuts at: 2182 2458 6308 6804 2182 Size: 276 3850 496 2483 Fragments arranged by size: 3850 2483 496 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6532 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4555 5316 6344 1540 Size: 275 186 2554 761 1028 2301 Fragments arranged by size: 2554 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3260 3596 3764 5166 5251 6322 3260 Size: 336 168 1402 85 1071 4043 Fragments arranged by size: 4043 1402 1071 336 168 85 BsmI GAATG_Cn' Cuts at: 2990 3624 3833 3986 4296 4616 4709 2990 Size: 634 209 153 310 320 93 5386 Fragments arranged by size: 5386 634 320 310 209 153 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2113 2181 3011 3260 3596 3764 Size: 399 269 54 68 830 249 336 168 Cuts at: 3764 4424 5166 5251 6322 1391 Size: 660 742 85 1071 2174 Fragments arranged by size: 2174 1071 830 742 660 399 336 269 249 168 85 68 54 BspEI T'CCGG_A Cuts at: 6768 6768 Size: 7105 BspHI T'CATG_A Cuts at: 4994 6724 4994 Size: 1730 5375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 4460 844 Size: 1478 2138 3489 Fragments arranged by size: 3489 2138 1478 BsrBI CCG'CTC Cuts at: 1394 1823 4992 1394 Size: 429 3169 3507 Fragments arranged by size: 3507 3169 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4107 5600 5774 65 Size: 1183 2859 1493 174 1396 Fragments arranged by size: 2859 1493 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5749 5936 1700 Size: 110 3939 187 2869 Fragments arranged by size: 3939 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3439 4195 96 Size: 3343 756 3006 Fragments arranged by size: 3343 3006 756 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7105 BssSI C'ACGA_G Cuts at: 5165 6177 5165 Size: 1012 6093 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7105 BstBI TT'CG_AA Cuts at: 2007 5909 2007 Size: 3902 3203 BstEII G'GTnAC_C Cuts at: 1184 2309 2797 1184 Size: 1125 488 5492 Fragments arranged by size: 5492 1125 488 BstYI r'GATC_y Cuts at: 1972 4082 4435 4802 5187 5204 6656 6742 6754 Size: 2110 353 367 385 17 1452 86 12 Cuts at: 6754 7100 1972 Size: 346 1977 Fragments arranged by size: 2110 1977 1452 385 367 353 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2346 Size: 7105 BtgI C'CryG_G Cuts at: 513 1065 2022 2039 2096 2226 2381 2491 513 Size: 552 957 17 57 130 155 110 5127 Fragments arranged by size: 5127 957 552 155 130 110 57 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6366 Fragments arranged by size: 6366 683 56 BtsI GCAGTG_nn' Cuts at: 3204 4327 4621 5415 5435 3204 Size: 1123 294 794 20 4874 Fragments arranged by size: 4874 1123 794 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7105 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2228 2263 3217 3252 3973 4008 529 Size: 35 1664 35 954 35 721 35 3626 Fragments arranged by size: 3626 1664 954 721 35 35 35 35 DraI TTT'AAA Cuts at: 3643 4409 3643 Size: 766 6339 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6112 809 Size: 507 4796 1802 Fragments arranged by size: 4796 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2223 4552 5441 Size: 54 1425 50 275 18 393 2329 889 Cuts at: 5441 7070 8 Size: 1629 43 Fragments arranged by size: 2329 1629 1425 889 393 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4552 1537 Size: 275 2740 4090 Fragments arranged by size: 4090 2740 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2238 2370 4380 5035 1353 Size: 885 132 2010 655 3423 Fragments arranged by size: 3423 2010 885 655 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5692 6066 6212 387 Size: 1064 138 4103 374 146 1280 Fragments arranged by size: 4103 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3258 3594 3762 3258 Size: 336 168 6601 Fragments arranged by size: 6601 336 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2318 2521 2895 3274 3312 3400 Size: 393 542 144 203 374 379 38 88 Cuts at: 3400 3610 3648 3732 3736 3778 3984 3988 4152 Size: 210 38 84 4 42 206 4 164 Cuts at: 4152 4236 5168 5765 6552 6820 1239 Size: 84 932 597 787 268 1524 Fragments arranged by size: 1524 932 787 597 542 393 379 374 268 210 206 203 164 144 88 84 84 42 38 38 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2156 Size: 7105 EcoO109I rG'GnC_Cy Cuts at: 2274 2274 Size: 7105 EcoRI G'AATT_C Cuts at: 2654 2949 3424 3450 4038 4264 4414 4792 2654 Size: 295 475 26 588 226 150 378 4967 Fragments arranged by size: 4967 588 475 378 295 226 150 26 FspI TGC'GCA Cuts at: 5611 5611 Size: 7105 HaeII r_GCGC'y Cuts at: 1678 6252 1678 Size: 4574 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2293 2325 2369 2401 3583 Size: 32 135 32 721 32 44 32 1182 Cuts at: 3583 3615 3751 3783 4169 4201 5746 5778 5820 Size: 32 136 32 386 32 1545 32 42 Cuts at: 5820 5852 1373 Size: 32 2626 Fragments arranged by size: 2626 1545 1182 721 386 136 135 44 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 4449 4695 669 Size: 988 281 154 2357 246 3079 Fragments arranged by size: 3079 2357 988 281 246 154 HindIII A'AGCT_T Cuts at: 748 3663 748 Size: 2915 4190 HpaI GTT'AAC Cuts at: 4695 4695 Size: 7105 KpnI G_GTAC'C Cuts at: 4430 4430 Size: 7105 MfeI C'AATT_G Cuts at: 4704 4704 Size: 7105 MluI A'CGCG_T Cuts at: 6981 6981 Size: 7105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2343 2484 2741 3022 6219 6403 1305 Size: 1038 141 257 281 3197 184 2007 Fragments arranged by size: 3197 2007 1038 281 257 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5283 Fragments arranged by size: 5283 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2934 3727 4047 4147 5063 5422 5581 Size: 648 1768 793 320 100 916 359 159 Cuts at: 5581 518 Size: 2042 Fragments arranged by size: 2042 1768 916 793 648 359 320 159 100 MspA1I CmG'CkG Cuts at: 1606 2087 2257 3420 3588 3756 4521 5198 6346 Size: 481 170 1163 168 168 765 677 1148 Cuts at: 6346 6591 1606 Size: 245 2120 Fragments arranged by size: 2120 1163 1148 765 677 481 245 170 168 168 NcoI C'CATG_G Cuts at: 513 1065 2022 2096 2491 513 Size: 552 957 74 395 5127 Fragments arranged by size: 5127 957 552 395 74 NdeI CA'TA_TG Cuts at: 387 3612 387 Size: 3225 3880 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7105 NotI GC'GGCC_GC Cuts at: 4552 4552 Size: 7105 NsiI A_TGCA'T Cuts at: 3624 4296 3624 Size: 672 6433 NspI r_CATG'y Cuts at: 1132 1229 2403 2753 4463 6008 1132 Size: 97 1174 350 1710 1545 2229 Fragments arranged by size: 2229 1710 1545 1174 350 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7105 PciI A'CATG_T Cuts at: 6004 6004 Size: 7105 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7105 PmeI GTTT'AAAC Cuts at: 4409 4409 Size: 7105 PmlI CAC'GTG Cuts at: 2140 2140 Size: 7105 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5174 5206 6687 6719 5174 Size: 32 1481 32 5560 Fragments arranged by size: 5560 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2274 2274 Size: 7105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7105 PsiI TTA'TAA Cuts at: 4675 4675 Size: 7105 PstI C_TGCA'G Cuts at: 830 3211 4457 830 Size: 2381 1246 3478 Fragments arranged by size: 3478 2381 1246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7105 PvuII CAG'CTG Cuts at: 2087 2257 3420 3588 3756 2087 Size: 170 1163 168 168 5436 Fragments arranged by size: 5436 1163 170 168 168 SacI G_AGCT'C Cuts at: 3260 3596 3764 3260 Size: 336 168 6601 Fragments arranged by size: 6601 336 168 SalI G'TCGA_C Cuts at: 1936 4447 1936 Size: 2511 4594 SapI GCTCTTCn'nnn_ Cuts at: 2370 2370 Size: 7105 SbfI CC_TGCA'GG Cuts at: 4457 4457 Size: 7105 ScaI AGT'ACT Cuts at: 2634 5353 7065 2634 Size: 2719 1712 2674 Fragments arranged by size: 2719 2674 1712 SfcI C'TryA_G Cuts at: 826 1045 3123 3207 3919 4093 4453 5588 6269 Size: 219 2078 84 712 174 360 1135 681 Cuts at: 6269 6460 826 Size: 191 1471 Fragments arranged by size: 2078 1471 1135 712 681 360 219 191 174 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7105 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7105 SmaI CCC'GGG Cuts at: 1872 4432 1872 Size: 2560 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5209 6110 6372 820 Size: 197 926 3266 901 262 1553 Fragments arranged by size: 3266 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4402 4896 493 Size: 3909 494 2702 Fragments arranged by size: 3909 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7105 SphI G_CATG'C Cuts at: 1132 4463 1132 Size: 3331 3774 SspI AAT'ATT Cuts at: 5 52 5029 5 Size: 47 4977 2081 Fragments arranged by size: 4977 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7105 StyI C'CwwG_G Cuts at: 513 1065 2022 2096 2491 2528 3029 4544 513 Size: 552 957 74 395 37 501 1515 3074 Fragments arranged by size: 3074 1515 957 552 501 395 74 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 2416 3042 5132 5149 5302 5487 Size: 1255 672 164 626 2090 17 153 185 Cuts at: 5487 5932 325 Size: 445 1498 Fragments arranged by size: 2090 1498 1255 672 626 445 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2594 2632 2894 Size: 274 80 33 51 1443 617 38 262 Cuts at: 2894 3439 3563 3731 4195 5351 7063 96 Size: 545 124 168 464 1156 1712 138 Fragments arranged by size: 1712 1443 1156 617 545 464 274 262 168 138 124 80 51 38 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7065 7097 7065 Size: 32 7073 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7105 XbaI T'CTAG_A Cuts at: 4441 4441 Size: 7105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2103 2103 Size: 7105 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7105 XmaI C'CCGG_G Cuts at: 1870 4430 1870 Size: 2560 4545 XmnI GAAnn'nnTTC Cuts at: 3497 3581 3706 5234 3497 Size: 84 125 1528 5368 Fragments arranged by size: 5368 1528 125 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3243 4913 276 Size: 53 83 186 936 1709 1670 2468 Fragments arranged by size: 2468 1709 1670 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI