(Circular) (Six-base) MAPSORT of: pFN21ASDA1810.seq Check: 6103 from: 1 to: 6424 pFN21ASDA1810 6424 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2861 2861 Size: 6424 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4234 278 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3767 1937 Size: 1830 4594 Acc65I G'GTAC_C Cuts at: 3745 3745 Size: 6424 AclI AA'CG_TT Cuts at: 1563 2260 4551 4924 1563 Size: 697 2291 373 3063 Fragments arranged by size: 3063 2291 697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2302 3465 4487 5871 6139 1632 Size: 670 1163 1022 1384 268 1917 Fragments arranged by size: 1917 1384 1163 1022 670 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6227 AflIII A'CryG_T Cuts at: 1203 5323 6300 1203 Size: 4120 977 1327 Fragments arranged by size: 4120 1327 977 AgeI A'CCGG_T Cuts at: 5255 5255 Size: 6424 AhdI GACnn_n'nnGTC Cuts at: 5153 5153 Size: 6424 AleI CACnn'nnGTG Cuts at: 1166 2036 2120 1166 Size: 870 84 5470 Fragments arranged by size: 5470 870 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6424 AlwNI CAG_nnn'CTG Cuts at: 3143 3179 3191 3209 3364 3385 3593 3617 3697 Size: 36 12 18 155 21 208 24 80 Cuts at: 3697 4167 5739 3143 Size: 470 1572 3828 Fragments arranged by size: 3828 1572 470 208 155 80 36 24 21 18 12 ApaI G_GGCC'C Cuts at: 3038 3465 3038 Size: 427 5997 ApaLI G'TGCA_C Cuts at: 2579 4481 5637 2579 Size: 1902 1156 3366 Fragments arranged by size: 3366 1902 1156 ApoI r'AATT_y Cuts at: 1493 3733 3959 4111 6220 1493 Size: 2240 226 152 2109 1697 Fragments arranged by size: 2240 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4978 160 Size: 872 3946 1606 Fragments arranged by size: 3946 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 AvaI C'yCGr_G Cuts at: 1870 1943 3749 1870 Size: 73 1806 4545 Fragments arranged by size: 4545 1806 73 BamHI G'GATC_C Cuts at: 3754 4121 3754 Size: 367 6057 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2771 3146 3681 3745 5200 Size: 325 217 627 984 375 535 64 1455 Cuts at: 5200 618 Size: 1842 Fragments arranged by size: 1842 1455 984 627 535 375 325 217 64 BanII G_rGCy'C Cuts at: 1391 2721 3038 3340 3465 3660 3675 3743 1391 Size: 1330 317 302 125 195 15 68 4072 Fragments arranged by size: 4072 1330 317 302 195 125 68 15 BbeI G_GCGC'C Cuts at: 3685 3685 Size: 6424 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2176 928 Size: 971 277 5176 Fragments arranged by size: 5176 971 277 BbvCI CC'TCA_GC Cuts at: 2382 2961 2382 Size: 579 5845 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4615 4649 1903 Size: 34 2678 34 3678 Fragments arranged by size: 3678 2678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2706 4316 5526 2706 Size: 1610 1210 3604 Fragments arranged by size: 3604 1610 1210 BclI T'GATC_A Cuts at: 1548 2571 1548 Size: 1023 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 3206 3469 Size: 107 122 71 631 605 385 1149 263 Cuts at: 3469 5035 136 Size: 1566 1525 Fragments arranged by size: 1566 1525 1149 631 605 385 263 122 107 71 BglII A'GATC_T Cuts at: 6419 6419 Size: 6424 BlpI GC'TnA_GC Cuts at: 3841 3841 Size: 6424 Bme1580I G_kGCm'C Cuts at: 1790 2583 3038 3297 3465 3632 4485 5641 1790 Size: 793 455 259 168 167 853 1156 2573 Fragments arranged by size: 2573 1156 853 793 455 259 168 167 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6424 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 4155 5113 Size: 416 53 83 399 24 1694 1044 958 Cuts at: 5113 442 Size: 1753 Fragments arranged by size: 1753 1694 1044 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2705 2988 3362 3386 3594 3719 5084 Size: 1211 255 283 374 24 208 125 1365 Cuts at: 5084 1239 Size: 2579 Fragments arranged by size: 2579 1365 1211 374 283 255 208 125 24 Bpu10I CC'TnA_GC Cuts at: 2382 2961 3067 2382 Size: 579 106 5739 Fragments arranged by size: 5739 579 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4549 5414 5712 4549 Size: 865 298 5261 Fragments arranged by size: 5261 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5087 882 Size: 617 3588 2219 Fragments arranged by size: 3588 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3721 4215 493 Size: 711 2517 494 2702 Fragments arranged by size: 2702 2517 711 494 BsaBI GATnn'nnATC Cuts at: 6418 6418 Size: 6424 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3411 3682 4231 4613 Size: 53 83 186 936 1878 271 549 382 Cuts at: 4613 5248 275 Size: 635 1451 Fragments arranged by size: 1878 1451 936 635 549 382 271 186 83 53 BsaWI w'CCGG_w Cuts at: 4857 5255 5529 5676 6087 4857 Size: 398 274 147 411 5194 Fragments arranged by size: 5194 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3063 3093 1178 Size: 30 165 30 1660 30 4509 Fragments arranged by size: 4509 1660 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2729 3075 3228 3341 3512 3520 3650 1186 Size: 1543 346 153 113 171 8 130 3960 Fragments arranged by size: 3960 1543 346 171 153 130 113 8 BseYI C'CCAG_C Cuts at: 2144 3062 3311 5627 6123 2144 Size: 918 249 2316 496 2445 Fragments arranged by size: 2445 2316 918 496 249 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 3120 1163 Size: 573 699 685 4467 Fragments arranged by size: 4467 699 685 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3874 4635 5663 1540 Size: 275 186 1873 761 1028 2301 Fragments arranged by size: 2301 1873 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2250 2427 2583 2721 3340 3675 4485 4570 5641 Size: 177 156 138 619 335 810 85 1071 Cuts at: 5641 2250 Size: 3033 Fragments arranged by size: 3033 1071 810 619 335 177 156 138 85 BsmI GAATG_Cn' Cuts at: 3935 4028 3935 Size: 93 6331 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2427 2583 2721 3038 3297 3340 Size: 399 460 177 156 138 317 259 43 Cuts at: 3340 3465 3632 3660 3675 3743 4485 4570 5641 Size: 125 167 28 15 68 742 85 1071 Cuts at: 5641 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 460 399 317 259 177 167 156 138 125 85 68 43 28 15 BspEI T'CCGG_A Cuts at: 6087 6087 Size: 6424 BspHI T'CATG_A Cuts at: 2559 2568 4313 6043 2559 Size: 9 1745 1730 2940 Fragments arranged by size: 2940 1745 1730 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2852 2861 3779 844 Size: 2008 9 918 3489 Fragments arranged by size: 3489 2008 918 9 BsrBI CCG'CTC Cuts at: 1394 1823 3355 4311 1394 Size: 429 1532 956 3507 Fragments arranged by size: 3507 1532 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2733 4919 5093 65 Size: 1183 1485 2186 174 1396 Fragments arranged by size: 2186 1485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3678 5068 5255 1700 Size: 110 1868 1390 187 2869 Fragments arranged by size: 2869 1868 1390 187 110 BsrGI T'GTAC_A Cuts at: 96 2160 2499 2547 96 Size: 2064 339 48 3973 Fragments arranged by size: 3973 2064 339 48 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6424 BssSI C'ACGA_G Cuts at: 4484 5496 4484 Size: 1012 5412 BstAPI GCAn_nnn'nTGC Cuts at: 794 2748 3385 794 Size: 1954 637 3833 Fragments arranged by size: 3833 1954 637 BstBI TT'CG_AA Cuts at: 2007 5228 2007 Size: 3221 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6424 BstXI CCAn_nnnn'nTGG Cuts at: 2617 2617 Size: 6424 BstYI r'GATC_y Cuts at: 1972 3754 4121 4506 4523 5975 6061 6073 6419 Size: 1782 367 385 17 1452 86 12 346 Cuts at: 6419 1972 Size: 1977 Fragments arranged by size: 1977 1782 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3645 3645 Size: 6424 BtgI C'CryG_G Cuts at: 513 1065 2457 3030 513 Size: 552 1392 573 3907 Fragments arranged by size: 3907 1392 573 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2862 487 Size: 683 56 1636 4049 Fragments arranged by size: 4049 1636 683 56 BtsI GCAGTG_nn' Cuts at: 2043 3401 3940 4734 4754 2043 Size: 1358 539 794 20 3713 Fragments arranged by size: 3713 1358 794 539 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6424 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2937 2972 3496 3531 529 Size: 35 2373 35 524 35 3422 Fragments arranged by size: 3422 2373 524 35 35 35 DraI TTT'AAA Cuts at: 3728 3728 Size: 6424 DraIII CAC_nnn'GTG Cuts at: 2458 2458 Size: 6424 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5431 809 Size: 507 4115 1802 Fragments arranged by size: 4115 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2619 3871 Size: 54 1425 50 275 18 219 570 1252 Cuts at: 3871 4760 6389 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1252 889 570 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3871 1537 Size: 275 2059 4090 Fragments arranged by size: 4090 2059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2345 2352 2360 2751 4354 1353 Size: 992 7 8 391 1603 3423 Fragments arranged by size: 3423 1603 992 391 8 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3665 5011 5385 5531 387 Size: 1064 138 2076 1346 374 146 1280 Fragments arranged by size: 2076 1346 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2719 3338 3673 2719 Size: 619 335 5470 Fragments arranged by size: 5470 619 335 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2302 2450 2705 2988 3362 3386 3465 Size: 393 670 148 255 283 374 24 79 Cuts at: 3465 3594 3719 4487 5084 5871 6139 1239 Size: 129 125 768 597 787 268 1524 Fragments arranged by size: 1524 787 768 670 597 393 374 283 268 255 148 129 125 79 24 EcoO109I rG'GnC_Cy Cuts at: 2983 3035 3461 3462 2983 Size: 52 426 1 5945 Fragments arranged by size: 5945 426 52 1 EcoRI G'AATT_C Cuts at: 3733 4111 3733 Size: 378 6046 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 6424 FspI TGC'GCA Cuts at: 4930 4930 Size: 6424 HaeII r_GCGC'y Cuts at: 1678 2063 3685 5571 1678 Size: 385 1622 1886 2531 Fragments arranged by size: 2531 1886 1622 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5065 5097 5139 5171 1373 Size: 32 135 32 3493 32 42 32 2626 Fragments arranged by size: 3493 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3487 3768 4014 669 Size: 988 281 1549 281 246 3079 Fragments arranged by size: 3079 1549 988 281 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6424 HpaI GTT'AAC Cuts at: 4014 4014 Size: 6424 KasI G'GCGC_C Cuts at: 3681 3681 Size: 6424 KpnI G_GTAC'C Cuts at: 3749 3749 Size: 6424 MfeI C'AATT_G Cuts at: 4023 4023 Size: 6424 MluI A'CGCG_T Cuts at: 6300 6300 Size: 6424 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5538 5722 1305 Size: 4233 184 2007 Fragments arranged by size: 4233 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2621 10 Size: 54 1425 343 789 3813 Fragments arranged by size: 3813 1425 789 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2120 2411 4382 4741 4900 518 Size: 648 870 84 291 1971 359 159 2042 Fragments arranged by size: 2042 1971 870 648 359 291 159 84 MspA1I CmG'CkG Cuts at: 1606 2811 2833 3140 3840 4517 5665 5910 1606 Size: 1205 22 307 700 677 1148 245 2120 Fragments arranged by size: 2120 1205 1148 700 677 307 245 22 NaeI GCC'GGC Cuts at: 3680 3680 Size: 6424 NarI GG'CG_CC Cuts at: 3682 3682 Size: 6424 NcoI C'CATG_G Cuts at: 513 1065 3030 513 Size: 552 1965 3907 Fragments arranged by size: 3907 1965 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6424 NgoMIV G'CCGG_C Cuts at: 3678 3678 Size: 6424 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6424 NotI GC'GGCC_GC Cuts at: 3871 3871 Size: 6424 NspI r_CATG'y Cuts at: 1132 1229 2506 3013 3782 5327 1132 Size: 97 1277 507 769 1545 2229 Fragments arranged by size: 2229 1545 1277 769 507 97 PasI CC'CwG_GG Cuts at: 1348 2481 2980 1348 Size: 1133 499 4792 Fragments arranged by size: 4792 1133 499 PciI A'CATG_T Cuts at: 5323 5323 Size: 6424 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 2728 3191 3209 1108 Size: 1269 351 463 18 4323 Fragments arranged by size: 4323 1269 463 351 18 PmeI GTTT'AAAC Cuts at: 3728 3728 Size: 6424 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4493 4525 6006 6038 4493 Size: 32 1481 32 4879 Fragments arranged by size: 4879 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6424 PsiI TTA'TAA Cuts at: 3994 3994 Size: 6424 PspOMI G'GGCC_C Cuts at: 3034 3461 3034 Size: 427 5997 PstI C_TGCA'G Cuts at: 830 3381 3426 3693 3776 830 Size: 2551 45 267 83 3478 Fragments arranged by size: 3478 2551 267 83 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6424 PvuII CAG'CTG Cuts at: 2811 3140 2811 Size: 329 6095 SacI G_AGCT'C Cuts at: 2721 3340 3675 2721 Size: 619 335 5470 Fragments arranged by size: 5470 619 335 SalI G'TCGA_C Cuts at: 1936 3766 1936 Size: 1830 4594 SbfI CC_TGCA'GG Cuts at: 3776 3776 Size: 6424 ScaI AGT'ACT Cuts at: 4672 6384 4672 Size: 1712 4712 SfcI C'TryA_G Cuts at: 826 1045 3377 3422 3510 3689 3772 4907 5588 Size: 219 2332 45 88 179 83 1135 681 Cuts at: 5588 5779 826 Size: 191 1471 Fragments arranged by size: 2332 1471 1135 681 219 191 179 88 83 45 SfiI GGCCn_nnn'nGGCC Cuts at: 3469 3469 Size: 6424 SfoI GGC'GCC Cuts at: 3683 3683 Size: 6424 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6424 SgrAI Cr'CCGG_yG Cuts at: 1700 3678 1700 Size: 1978 4446 SmaI CCC'GGG Cuts at: 1872 3751 1872 Size: 1879 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4528 5429 5691 820 Size: 197 926 2585 901 262 1553 Fragments arranged by size: 2585 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3721 4215 493 Size: 3228 494 2702 Fragments arranged by size: 3228 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6424 SphI G_CATG'C Cuts at: 1132 3782 1132 Size: 2650 3774 SspI AAT'ATT Cuts at: 5 52 4348 5 Size: 47 4296 2081 Fragments arranged by size: 4296 2081 47 StuI AGG'CCT Cuts at: 1517 2171 3607 1517 Size: 654 1436 4334 Fragments arranged by size: 4334 1436 654 StyI C'CwwG_G Cuts at: 513 1065 3030 3456 3863 513 Size: 552 1965 426 407 3074 Fragments arranged by size: 3074 1965 552 426 407 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4451 4468 4621 4806 5251 325 Size: 1255 2871 17 153 185 445 1498 Fragments arranged by size: 2871 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2160 2499 2547 Size: 274 80 33 51 1443 183 339 48 Cuts at: 2547 4670 6382 96 Size: 2123 1712 138 Fragments arranged by size: 2123 1712 1443 339 274 183 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2680 2712 3063 3095 6384 6416 2680 Size: 32 351 32 3289 32 2688 Fragments arranged by size: 3289 2688 351 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2134 2735 1316 Size: 818 601 5005 Fragments arranged by size: 5005 818 601 XbaI T'CTAG_A Cuts at: 3760 3760 Size: 6424 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 2857 2146 Size: 711 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6424 XmaI C'CCGG_G Cuts at: 1870 3749 1870 Size: 1879 4545 XmnI GAAnn'nnTTC Cuts at: 4553 4553 Size: 6424 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4232 276 Size: 53 83 186 936 2698 2468 Fragments arranged by size: 2698 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI