Restriction map for
pFN21ASDA1612
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccII AccIII AclI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BamHI
BbrPI BcgI BciVI BclI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FauI FbaI FseI
FspI HincII HindII HpaI KspI
MfeI MflI MluI MroI MunI
MvnI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 538 GGTACC G^GTACC
AccI 1507 GTCTAC GT^[AC][GT]AC
AflII 895 CTTAAG C^TTAAG
AgeI 1330 ACCGGT A^CCGGT
Asp718I 538 GGTACC G^GTACC
AvaI 35 CCCGGG C^[CT]CG[GA]G
BalI 1211 TGGCCA TGG^CCA
BbeI 482 GGCGCC GGCGC^C
BbsI 1326 GAAGAC GAAGAC(2/6)
BfrI 895 CTTAAG C^TTAAG
BpmI 126 CTGGAG CTGGAG(16/14)
BpuAI 1326 GAAGAC GAAGAC(2/6)
BsaBI 1209 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 997 GAGGAG GAGGAG(10/8)
BsoBI 35 CCCGGG C^[CT]CG[GA]G
Bsp1407I 551 TGTACA T^GTACA
BspMI 111 GCAGGT ACCTGC(4/8)
BsrFI 1330 ACCGGT [GA]^CCGG[CT]
BsrGI 551 TGTACA T^GTACA
BsrI 618 ACTGG ACTGG(1/-1)
Cac8I 15 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 483 GCGC GCG^C
Cfr10I 1330 ACCGGT [GA]^CCGG[CT]
DraII 963 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 518 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 963 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 439 GAATTC G^AATTC
EcoRV 1156 GATATC GAT^ATC
HaeII 482 GGCGCC [GA]GCGC^[CT]
HhaI 483 GCGC GCG^C
HinP1I 483 GCGC G^CGC
KasI 482 GGCGCC G^GCGCC
KpnI 538 GGTACC GGTAC^C
MaeII 687 ACGT A^CGT
MamI 1209 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1211 TGGCCA TGG^CCA
MscI 1211 TGGCCA TGG^CCA
MspA1I 17 CAGCGG C[AC]G^C[GT]G
NarI 482 GGCGCC GG^CGCC
PflMI 1417 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1330 ACCGGT A^CCGGT
PpuMI 963 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 1462 GAAGAGC GCTCTTC(1/4)
SfaNI 448 GATGC GCATC(5/9)
SfcI 1509 CTACAG C^T[GA][CT]AG
SfoI 482 GGCGCC GGC^GCC
SmaI 35 CCCGGG CCC^GGG
SmlI 895 CTTAAG C^T[CT][GA]AG
SspBI 551 TGTACA T^GTACA
Van91I 1417 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 35 CCCGGG C^CCGGG
XmaI 35 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 138 GACGCC G[GA]^CG[CT]C
482 GGCGCC
AflIII 419 ACATGT A^C[GA][CT]GT
942 ACATGT
AlwI 195 GATCC GGATC(4/5)
806 GGATC
BanI 482 GGCGCC G^G[CT][GA]CC
538 GGTACC
BanII 22 GAGCCC G[GA]GC[CT]^C
765 GAGCCC
BbvCI 118 GCTGAGG CCTCAGC(-5/-2)
994 GCTGAGG
BfaI 1175 CTAG C^TAG
1378 CTAG
BmyI 22 GAGCCC G[AGT]GC[ACT]^C
765 GAGCCC
BsaHI 138 GACGCC G[GA]^CG[CT]C
482 GGCGCC
BsaWI 368 TCCGGT [AT]^CCGG[AT]
1330 ACCGGT
Bsp1286I 22 GAGCCC G[AGT]GC[ACT]^C
765 GAGCCC
BspHI 786 TCATGA T^CATGA
1350 TCATGA
BspLU11I 419 ACATGT A^CATGT
942 ACATGT
BsrDI 1443 CATTGC GCAATG(2/0)
1238 GCAATG
DraIII 187 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
652 CACCAGGTG
EaeI 13 CGGCCA [CT]^GGCC[GA]
1211 TGGCCA
EcoT14I 929 CCTTGG C^C[AT][AT]GG
1305 CCAAGG
HgaI 82 GACGC GACGC(5/10)
138 GACGC
Hin1I 138 GACGCC G[GA]^CG[CT]C
482 GGCGCC
HindIII 1006 AAGCTT A^AGCTT
1090 AAGCTT
MaeI 1175 CTAG C^TAG
1378 CTAG
NspI 419 ACATGT [GA]CATG^[CT]
942 ACATGT
PleI 726 GACTC GAGTC(4/5)
1524 GACTC
RcaI 786 TCATGA T^CATGA
1350 TCATGA
SexAI 506 ACCTGGT A^CC[AT]GGT
653 ACCAGGT
StyI 929 CCTTGG C^C[AT][AT]GG
1305 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AvaII BbvI BcnI BsaJI
BsiYI BslI BsmAI BsmFI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI StuI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI