Restriction map for
pFN21ASDA1612
(Circular) (Six-base) MAPSORT of: pFN21ASDA1612.seq Check: 1178 from: 1 to: 6355
pFN21ASDA1612 6355 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2125 2125
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3531 3698 1937
Size: 1594 167 4594
Fragments arranged by size:
4594 1594 167
Acc65I G'GTAC_C
Cuts at: 2561 3676 2561
Size: 1115 5240
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2243 2706 2718 2927 3131 3207 4418 5802
Size: 611 463 12 209 204 76 1211 1384
Cuts at: 5802 6070 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1211 611 463 268 209 204
76 12
AflII C'TTAA_G
Cuts at: 820 1017 2918 820
Size: 197 1901 4257
Fragments arranged by size:
4257 1901 197
AflIII A'CryG_T
Cuts at: 1203 2442 2965 5254 6231 1203
Size: 1239 523 2289 977 1327
Fragments arranged by size:
2289 1327 1239 977 523
AgeI A'CCGG_T
Cuts at: 3353 5186 3353
Size: 1833 4522
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 4098 5670 4098
Size: 1572 4783
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2373 2462 2868 2898 2930 3392 3554 3664
Size: 880 89 406 30 32 462 162 110
Cuts at: 3664 3890 4042 6151 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 880 462 406 226 162 152
110 89 32 30
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 3680 1870
Size: 73 115 1622 4545
Fragments arranged by size:
4545 1622 115 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2505 2561 3676 5131 618
Size: 325 217 627 718 56 1115 1455 1842
Fragments arranged by size:
1842 1455 1115 718 627 325 217 56
BanII G_rGCy'C
Cuts at: 1391 2049 2792 3674 1391
Size: 658 743 882 4072
Fragments arranged by size:
4072 882 743 658
BbeI G_GCGC'C
Cuts at: 2509 2509
Size: 6355
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3356 928
Size: 971 1457 3927
Fragments arranged by size:
3927 1457 971
BbvCI CC'TCA_GC
Cuts at: 2142 3018 2142
Size: 876 5479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4966 136
Size: 107 122 71 631 605 3294 1525
Fragments arranged by size:
3294 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 4416 5572 1790
Size: 2626 1156 2573
Fragments arranged by size:
2626 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4086 5044 442
Size: 416 53 83 399 24 2669 958 1753
Fragments arranged by size:
2669 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2170 5015 1239
Size: 931 2845 2579
Fragments arranged by size:
2845 2579 931
Bpu10I CC'TnA_GC
Cuts at: 2142 3018 3213 2142
Size: 876 195 5284
Fragments arranged by size:
5284 876 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5018 882
Size: 617 3519 2219
Fragments arranged by size:
3519 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3652 4146 493
Size: 711 2448 494 2702
Fragments arranged by size:
2702 2448 711 494
BsaBI GATnn'nnATC
Cuts at: 3236 6349 3236
Size: 3113 3242
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2162 2506 4162 4544
Size: 53 83 186 936 629 344 1656 382
Cuts at: 4544 5179 275
Size: 635 1451
Fragments arranged by size:
1656 1451 936 635 629 382 344 186
83 53
BsaWI w'CCGG_w
Cuts at: 2391 3353 4788 5186 5460 5607 6018 2391
Size: 962 1435 398 274 147 411 2728
Fragments arranged by size:
2728 1435 962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2542 2572 2740 2770 1178
Size: 30 165 30 1139 30 168 30 4763
Fragments arranged by size:
4763 1139 168 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3035 1186
Size: 1849 4506
BseYI C'CCAG_C
Cuts at: 2053 3432 5558 6054 2053
Size: 1379 2126 496 2354
Fragments arranged by size:
2354 2126 1379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5782
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4566 5594 1540
Size: 275 186 1804 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4416 4501 5572 4416
Size: 85 1071 5199
Fragments arranged by size:
5199 1071 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2792 3674 4416 4501 5572 1391
Size: 399 259 743 882 742 85 1071 2174
Fragments arranged by size:
2174 1071 882 743 742 399 259 85
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 2809 3373 4244 5974 2809
Size: 564 871 1730 3190
Fragments arranged by size:
3190 1730 871 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2125 3710 844
Size: 1281 1585 3489
Fragments arranged by size:
3489 1585 1281
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3268 3465 4850 5024 65
Size: 1183 2020 197 1385 174 1396
Fragments arranged by size:
2020 1396 1385 1183 197 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3353 4999 5186 1700
Size: 110 1543 1646 187 2869
Fragments arranged by size:
2869 1646 1543 187 110
BsrGI T'GTAC_A
Cuts at: 96 2574 96
Size: 2478 3877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 2007 5159 2007
Size: 3152 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350
Size: 1713 367 385 17 1452 86 12 346
Cuts at: 6350 1972
Size: 1977
Fragments arranged by size:
1977 1713 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5803
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 3871 4665 4685 3871
Size: 794 20 5541
Fragments arranged by size:
5541 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DraIII CAC_nnn'GTG
Cuts at: 2215 2680 2215
Size: 465 5890
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 3234 3802
Size: 54 1425 50 275 18 206 1198 568
Cuts at: 3802 4691 6320 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1198 889 568 275 206 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2338 2871 3480 4285 1353
Size: 980 5 533 609 805 3423
Fragments arranged by size:
3423 980 805 609 533 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 4942 5316 5462 387
Size: 1064 138 519 2834 374 146 1280
Fragments arranged by size:
2834 1280 1064 519 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2170 2243 2706 2718 2927 3131 3207
Size: 393 538 73 463 12 209 204 76
Cuts at: 3207 4418 5015 5802 6070 1239
Size: 1211 597 787 268 1524
Fragments arranged by size:
1524 1211 787 597 538 463 393 268
209 204 76 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2545 2545
Size: 6355
EcoO109I rG'GnC_Cy
Cuts at: 2987 2987
Size: 6355
EcoRI G'AATT_C
Cuts at: 2462 3664 4042 2462
Size: 1202 378 4775
Fragments arranged by size:
4775 1202 378
EcoRV GAT'ATC
Cuts at: 3181 3181
Size: 6355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3534 3616 3534
Size: 82 6273
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 2509 5502 1678
Size: 831 2993 2531
Fragments arranged by size:
2993 2531 831
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2378 2410 2414 2446 2740
Size: 32 135 32 806 32 4 32 294
Cuts at: 2740 2772 4996 5028 5070 5102 1373
Size: 32 2224 32 42 32 2626
Fragments arranged by size:
2626 2224 806 294 135 42 32 32
32 32 32 32 32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3029 3113 748
Size: 2281 84 3990
Fragments arranged by size:
3990 2281 84
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KasI G'GCGC_C
Cuts at: 2505 2505
Size: 6355
KpnI G_GTAC'C
Cuts at: 2565 3680 2565
Size: 1115 5240
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3120 5469 5653 1305
Size: 1815 2349 184 2007
Fragments arranged by size:
2349 2007 1815 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 10
Size: 54 1425 343 1404 3129
Fragments arranged by size:
3129 1425 1404 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2192 2441 2490 2964 4313 4672
Size: 648 1020 6 249 49 474 1349 359
Cuts at: 4672 4831 518
Size: 159 2042
Fragments arranged by size:
2042 1349 1020 648 474 359 249 159
49 6
MspA1I CmG'CkG
Cuts at: 1606 2042 3771 4448 5596 5841 1606
Size: 436 1729 677 1148 245 2120
Fragments arranged by size:
2120 1729 1148 677 436 245
NarI GG'CG_CC
Cuts at: 2506 2506
Size: 6355
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5803
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2446 2969 3713 5258 1132
Size: 97 1217 523 744 1545 2229
Fragments arranged by size:
2229 1545 1217 744 523 97
PasI CC'CwG_GG
Cuts at: 1348 2541 1348
Size: 1193 5162
PciI A'CATG_T
Cuts at: 2442 2965 5254 2442
Size: 523 2289 3543
Fragments arranged by size:
3543 2289 523
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3446 1108
Size: 2338 4017
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 5937 5969 4424
Size: 32 1481 32 4810
Fragments arranged by size:
4810 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2987 2987
Size: 6355
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2763 2795 2763
Size: 32 6323
PstI C_TGCA'G
Cuts at: 830 3707 830
Size: 2877 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SapI GCTCTTCn'nnn_
Cuts at: 3480 3480
Size: 6355
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SexAI A'CCwGG_T
Cuts at: 2529 2676 2529
Size: 147 6208
SfcI C'TryA_G
Cuts at: 826 1045 3532 3703 4838 5519 5710 826
Size: 219 2487 171 1135 681 191 1471
Fragments arranged by size:
2487 1471 1135 681 219 191 171
SfoI GGC'GCC
Cuts at: 2507 2507
Size: 6355
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 2060 3682 1872
Size: 188 1622 4545
Fragments arranged by size:
4545 1622 188
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 4459 5360 5622 820
Size: 197 926 975 1541 901 262 1553
Fragments arranged by size:
1553 1541 975 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3652 4146 493
Size: 3159 494 2702
Fragments arranged by size:
3159 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 2147 2951 3108 1517
Size: 630 804 157 4764
Fragments arranged by size:
4764 804 630 157
StyI C'CwwG_G
Cuts at: 513 1065 2952 3328 3794 513
Size: 552 1887 376 466 3074
Fragments arranged by size:
3074 1887 552 466 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2546 4382 4399 4552 4737 5182 325
Size: 1255 966 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 966 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2574 3414 4601
Size: 274 80 33 51 1443 597 840 1187
Cuts at: 4601 6313 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1187 840 597 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2542 2574 6315 6347 2542
Size: 32 3741 32 2550
Fragments arranged by size:
3741 2550 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 2058 3680 1870
Size: 188 1622 4545
Fragments arranged by size:
4545 1622 188
XmnI GAAnn'nnTTC
Cuts at: 2256 2592 3599 4484 2256
Size: 336 1007 885 4127
Fragments arranged by size:
4127 1007 885 336
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI
PspXI PvuII RsrII SacI SacII SanDI SfiI SrfI
SwaI XcmI