Restriction map for
pFN21ASDA1580
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AvaI AviII BbeI BbrPI
BbvCI BclI BfrI BglI BglII
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsoBI BspDI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstUI Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tth111I Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 210 TCCGGA T^CCGGA
AflIII 1273 ACATGT A^C[GA][CT]GT
AlwNI 1523 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 59 GGGCCC GGGCC^C
AseI 1705 ATTAAT AT^TAAT
AsnI 1705 ATTAAT AT^TAAT
AvrII 46 CCTAGG C^CTAGG
BalI 1601 TGGCCA TGG^CCA
BamHI 1544 GGATCC G^GATCC
BanI 571 GGTGCC G^G[CT][GA]CC
BbsI 1569 GAAGAC GAAGAC(2/6)
BcgI 1656 GCAGCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 884 GGATAC GTATCC(6/5)
BcnI 983 CCCGG CC^[GC]GG
BlnI 46 CCTAGG C^CTAGG
BpmI 1436 CTCCAG CTGGAG(16/14)
BpuAI 1569 GAAGAC GAAGAC(2/6)
BsaI 1453 GAGACC GGTCTC(1/5)
BseAI 210 TCCGGA T^CCGGA
BseRI 995 CTCCTC GAGGAG(10/8)
BsmBI 1391 CGTCTC CGTCTC(1/5)
BsmI 780 GAATGC GAATGC(1/-1)
BspEI 210 TCCGGA T^CCGGA
BspLU11I 1273 ACATGT A^CATGT
Bst1107I 433 GTATAC GTA^TAC
BstXI 1433 CCACTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 433 GTATAC GTA^TAC
CfoI 522 GCGC GCG^C
DrdI 1084 GACCTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1739 CCATGG C^C[GA][CT]GG
EaeI 1601 TGGCCA [CT]^GGCC[GA]
EcoNI 198 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 509 GAATTC G^AATTC
FauI 126 GCGGG CCCGC(4/6)
HhaI 522 GCGC GCG^C
HinP1I 522 GCGC G^CGC
HpaI 1831 GTTAAC GTT^AAC
MaeII 1228 ACGT A^CGT
MluNI 1601 TGGCCA TGG^CCA
MroI 210 TCCGGA T^CCGGA
MscI 1601 TGGCCA TGG^CCA
NciI 983 CCCGG CC^[GC]GG
NcoI 1739 CCATGG C^CATGG
PleI 1911 GACTC GAGTC(4/5)
PshAI 397 GACAATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1705 ATTAAT AT^TAAT
SapI 94 GCTCTTC GCTCTTC(1/4)
SpeI 804 ACTAGT A^CTAGT
StuI 703 AGGCCT AGG^CCT
XbaI 876 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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BlpI 933 GCTCAGC GC^T[ACGT]AGC
1111 GCTGAGC
Bpu1102I 933 GCTCAGC GC^T[ACGT]AGC
1111 GCTGAGC
BsaWI 210 TCCGGA [AT]^CCGG[AT]
1059 TCCGGT
BsmFI 756 GTCCC GGGAC(10/14)
1430 GTCCC
Bsp1407I 1268 TGTACA T^GTACA
1902 TGTACA
BsrBI 649 CCGCTC CCGCTC(-3/-3)
880 GAGCGG
BsrGI 1268 TGTACA T^GTACA
1902 TGTACA
BstYI 672 GGATCT [GA]^GATC[CT]
1544 GGATCC
CelII 933 GCTCAGC GC^T[ACGT]AGC
1111 GCTGAGC
EarI 95 CTCTTC CTCTTC(1/4)
856 CTCTTC
Ksp632I 95 CTCTTC CTCTTC(1/4)
856 CTCTTC
MflI 672 GGATCT [GA]^GATC[CT]
1544 GGATCC
MspA1I 936 CAGCTG C[AC]G^C[GT]G
1109 CAGCTG
PpuMI 42 AGGTCCT [GA]G^G[AT]CC[CT]
1428 GGGTCCC
PvuII 936 CAGCTG CAG^CTG
1109 CAGCTG
SacI 454 GAGCTC GAGCT^C
1027 GAGCTC
SalI 1444 GTCGAC G^TCGAC
1497 GTCGAC
SmlI 294 CTTGAG C^T[CT][GA]AG
594 CTTGAG
SspBI 1268 TGTACA T^GTACA
1902 TGTACA
TfiI 684 GAATC G^A[AT]TC
762 GATTC
XcmI 1438 CCAGTGGTCGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1604 CCATCACACTCATGG
XhoII 672 GGATCT [GA]^GATC[CT]
1544 GGATCC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbvI BfaI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI