(Circular) (Six-base) MAPSORT of: pFN21ASDA1580.seq Check: 6480 from: 1 to: 6649 pFN21ASDA1580 6649 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3783 3783 Size: 6649 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4459 278 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2457 3468 3521 3992 1937 Size: 520 1011 53 471 4594 Fragments arranged by size: 4594 1011 520 471 53 Acc65I G'GTAC_C Cuts at: 3970 3970 Size: 6649 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2492 2804 3141 3318 4712 6096 6364 1632 Size: 860 312 337 177 1394 1384 268 1917 Fragments arranged by size: 1917 1394 1384 860 337 312 268 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 3296 5548 6525 1203 Size: 2093 2252 977 1327 Fragments arranged by size: 2252 2093 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 5378 5378 Size: 6649 AleI CACnn'nnGTG Cuts at: 1166 3461 3485 1166 Size: 2295 24 4330 Fragments arranged by size: 4330 2295 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6649 AlwNI CAG_nnn'CTG Cuts at: 3551 4392 5964 3551 Size: 841 1572 4236 Fragments arranged by size: 4236 1572 841 ApaI G_GGCC'C Cuts at: 2086 2086 Size: 6649 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 2532 3256 3865 3958 4184 4336 6445 1493 Size: 1039 724 609 93 226 152 2109 1697 Fragments arranged by size: 2109 1697 1039 724 609 226 152 93 AseI AT'TA_AT Cuts at: 160 1032 3729 5203 160 Size: 872 2697 1474 1606 Fragments arranged by size: 2697 1606 1474 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 3974 1870 Size: 73 2031 4545 Fragments arranged by size: 4545 2031 73 AvrII C'CTAG_G Cuts at: 2069 2069 Size: 6649 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3156 3189 3156 Size: 33 6616 BamHI G'GATC_C Cuts at: 3567 3979 4346 3567 Size: 412 367 5870 Fragments arranged by size: 5870 412 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 3970 5425 618 Size: 325 217 627 807 1376 1455 1842 Fragments arranged by size: 1842 1455 1376 807 627 325 217 BanII G_rGCy'C Cuts at: 1391 2086 2155 2481 3054 3099 3968 1391 Size: 695 69 326 573 45 869 4072 Fragments arranged by size: 4072 869 695 573 326 69 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3599 928 Size: 971 1700 3978 Fragments arranged by size: 3978 1700 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3668 3702 4840 4874 1903 Size: 34 1731 34 1138 34 3678 Fragments arranged by size: 3678 1731 1138 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2901 4541 5751 2901 Size: 1640 1210 3799 Fragments arranged by size: 3799 1640 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5260 136 Size: 107 122 71 631 605 3588 1525 Fragments arranged by size: 3588 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6644 6644 Size: 6649 BlpI GC'TnA_GC Cuts at: 2957 3135 4066 2957 Size: 178 931 5540 Fragments arranged by size: 5540 931 178 Bme1580I G_kGCm'C Cuts at: 1790 2086 3007 4710 5866 1790 Size: 296 921 1703 1156 2573 Fragments arranged by size: 2573 1703 1156 921 296 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6649 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3481 3539 4380 Size: 416 53 83 399 24 2064 58 841 Cuts at: 4380 5338 442 Size: 958 1753 Fragments arranged by size: 2064 1753 958 841 416 399 83 58 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3444 5309 1239 Size: 2205 1865 2579 Fragments arranged by size: 2579 2205 1865 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2338 2638 4774 5639 5937 2338 Size: 300 2136 865 298 3050 Fragments arranged by size: 3050 2136 865 300 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3470 5312 882 Size: 617 1971 1842 2219 Fragments arranged by size: 2219 1971 1842 617 BsaAI yAC'GTr Cuts at: 493 1204 3946 4440 493 Size: 711 2742 494 2702 Fragments arranged by size: 2742 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6643 6643 Size: 6649 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4456 4838 5473 275 Size: 53 83 186 936 2923 382 635 1451 Fragments arranged by size: 2923 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2233 3082 5082 5480 5754 5901 6312 2233 Size: 849 2000 398 274 147 411 2570 Fragments arranged by size: 2570 2000 849 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2439 2468 2469 2498 3352 Size: 30 165 30 1036 29 1 29 854 Cuts at: 3352 3382 1178 Size: 30 4445 Fragments arranged by size: 4445 1036 854 165 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3009 1186 Size: 1823 4826 BseYI C'CCAG_C Cuts at: 5852 6348 5852 Size: 496 6153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6076 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4099 4860 5888 1540 Size: 275 186 2098 761 1028 2301 Fragments arranged by size: 2301 2098 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2481 3054 3240 3515 3804 4710 4795 5866 Size: 304 573 186 275 289 906 85 1071 Cuts at: 5866 2177 Size: 2960 Fragments arranged by size: 2960 1071 906 573 304 289 275 186 85 BsmI GAATG_Cn' Cuts at: 2809 4160 4253 2809 Size: 1351 93 5205 Fragments arranged by size: 5205 1351 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3420 3420 Size: 6649 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2155 2177 2481 3007 3054 3099 Size: 399 296 69 22 304 526 47 45 Cuts at: 3099 3240 3515 3804 3968 4710 4795 5866 1391 Size: 141 275 289 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 304 296 289 275 164 141 85 69 47 45 22 BspEI T'CCGG_A Cuts at: 2233 6312 2233 Size: 4079 2570 BspHI T'CATG_A Cuts at: 2872 3201 3588 4538 6268 2872 Size: 329 387 950 1730 3253 Fragments arranged by size: 3253 1730 950 387 329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2168 2229 3783 4004 844 Size: 1324 61 1554 221 3489 Fragments arranged by size: 3489 1554 1324 221 61 BsrBI CCG'CTC Cuts at: 1394 1823 2674 2905 4536 1394 Size: 429 851 231 1631 3507 Fragments arranged by size: 3507 1631 851 429 231 BsrDI GCAATG_nn' Cuts at: 65 1248 5144 5318 65 Size: 1183 3896 174 1396 Fragments arranged by size: 3896 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5293 5480 1700 Size: 110 3483 187 2869 Fragments arranged by size: 3483 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3291 3925 96 Size: 3195 634 2820 Fragments arranged by size: 3195 2820 634 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 4709 5721 4709 Size: 1012 5637 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6649 BstBI TT'CG_AA Cuts at: 2007 5453 2007 Size: 3446 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6649 BstXI CCAn_nnnn'nTGG Cuts at: 3463 3463 Size: 6649 BstYI r'GATC_y Cuts at: 1972 2695 3567 3979 4346 4731 4748 6200 6286 Size: 723 872 412 367 385 17 1452 86 Cuts at: 6286 6298 6644 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 872 723 412 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2458 2458 Size: 6649 BtgI C'CryG_G Cuts at: 513 1065 3762 513 Size: 552 2697 3400 Fragments arranged by size: 3400 2697 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5910 Fragments arranged by size: 5910 683 56 BtsI GCAGTG_nn' Cuts at: 3651 4165 4959 4979 3651 Size: 514 794 20 5321 Fragments arranged by size: 5321 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2747 2782 529 Size: 35 2183 35 4396 Fragments arranged by size: 4396 2183 35 35 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3113 5656 809 Size: 507 1797 2543 1802 Fragments arranged by size: 2543 1802 1797 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3624 4096 4985 Size: 54 1425 50 275 18 1794 472 889 Cuts at: 4985 6614 8 Size: 1629 43 Fragments arranged by size: 1794 1629 1425 889 472 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2124 2885 4579 1353 Size: 771 761 1694 3423 Fragments arranged by size: 3423 1694 771 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5236 5610 5756 387 Size: 1064 138 3647 374 146 1280 Fragments arranged by size: 3647 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2479 3052 2479 Size: 573 6076 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2804 3141 3318 3444 4712 5309 Size: 393 860 312 337 177 126 1268 597 Cuts at: 5309 6096 6364 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1268 860 787 597 393 337 312 268 177 126 EcoNI CCTnn'n_nnAGG Cuts at: 2225 2225 Size: 6649 EcoO109I rG'GnC_Cy Cuts at: 2066 2082 2083 3452 2066 Size: 16 1 1369 5263 Fragments arranged by size: 5263 1369 16 1 EcoRI G'AATT_C Cuts at: 2532 3958 4336 2532 Size: 1426 378 4845 Fragments arranged by size: 4845 1426 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 2529 2056 Size: 473 6176 FspI TGC'GCA Cuts at: 5155 5155 Size: 6649 HaeII r_GCGC'y Cuts at: 1678 5796 1678 Size: 4118 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2228 2260 3481 Size: 32 135 32 457 32 167 32 1221 Cuts at: 3481 3513 5290 5322 5364 5396 1373 Size: 32 1777 32 42 32 2626 Fragments arranged by size: 2626 1777 1221 457 167 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3469 3522 3856 3993 4239 669 Size: 988 281 1531 53 334 137 246 3079 Fragments arranged by size: 3079 1531 988 334 281 246 137 53 HindIII A'AGCT_T Cuts at: 748 748 Size: 6649 HpaI GTT'AAC Cuts at: 3856 4239 3856 Size: 383 6266 KpnI G_GTAC'C Cuts at: 3974 3974 Size: 6649 MfeI C'AATT_G Cuts at: 4248 4248 Size: 6649 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2637 5763 5947 1305 Size: 1332 3126 184 2007 Fragments arranged by size: 3126 2007 1332 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3626 10 Size: 54 1425 343 1794 3033 Fragments arranged by size: 3033 1794 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 3299 3461 3485 3545 3635 3761 Size: 648 1131 1002 162 24 60 90 126 Cuts at: 3761 4607 4966 5125 518 Size: 846 359 159 2042 Fragments arranged by size: 2042 1131 1002 846 648 359 162 159 126 90 60 24 MspA1I CmG'CkG Cuts at: 1606 2961 3134 4065 4742 5890 6135 1606 Size: 1355 173 931 677 1148 245 2120 Fragments arranged by size: 2120 1355 1148 931 677 245 173 NcoI C'CATG_G Cuts at: 513 1065 3762 513 Size: 552 2697 3400 Fragments arranged by size: 3400 2697 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 3075 3292 3300 4007 5552 1132 Size: 97 1846 217 8 707 1545 2229 Fragments arranged by size: 2229 1846 1545 707 217 97 8 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6649 PciI A'CATG_T Cuts at: 3296 5548 3296 Size: 2252 4397 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6649 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2066 3452 2066 Size: 1386 5263 PshAI GACnn'nnGTC Cuts at: 1613 2424 1613 Size: 811 5838 PsiI TTA'TAA Cuts at: 4219 4219 Size: 6649 PspOMI G'GGCC_C Cuts at: 2082 2082 Size: 6649 PstI C_TGCA'G Cuts at: 830 2183 3106 3358 3646 4001 830 Size: 1353 923 252 288 355 3478 Fragments arranged by size: 3478 1353 923 355 288 252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 PvuII CAG'CTG Cuts at: 2961 3134 2961 Size: 173 6476 SacI G_AGCT'C Cuts at: 2481 3054 2481 Size: 573 6076 SalI G'TCGA_C Cuts at: 1936 3467 3520 3991 1936 Size: 1531 53 471 4594 Fragments arranged by size: 4594 1531 471 53 SanDI GG'GwC_CC Cuts at: 3452 3452 Size: 6649 SapI GCTCTTCn'nnn_ Cuts at: 2124 2124 Size: 6649 SbfI CC_TGCA'GG Cuts at: 4001 4001 Size: 6649 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SfcI C'TryA_G Cuts at: 826 1045 2179 2336 3102 3354 3642 3997 5132 Size: 219 1134 157 766 252 288 355 1135 Cuts at: 5132 5813 6004 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1134 766 681 355 288 252 219 191 157 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3976 1872 Size: 2104 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2317 2617 4753 5654 5916 820 Size: 197 926 374 300 2136 901 262 1553 Fragments arranged by size: 2136 1553 926 901 374 300 262 197 SnaBI TAC'GTA Cuts at: 493 3946 4440 493 Size: 3453 494 2702 Fragments arranged by size: 3453 2702 494 SpeI A'CTAG_T Cuts at: 152 2827 152 Size: 2675 3974 SphI G_CATG'C Cuts at: 1132 4007 1132 Size: 2875 3774 SspI AAT'ATT Cuts at: 5 52 4573 5 Size: 47 4521 2081 Fragments arranged by size: 4521 2081 47 StuI AGG'CCT Cuts at: 1517 2728 1517 Size: 1211 5438 StyI C'CwwG_G Cuts at: 513 1065 2069 2927 3762 4088 513 Size: 552 1004 858 835 326 3074 Fragments arranged by size: 3074 1004 858 835 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3603 3775 4676 4693 4846 5031 5476 Size: 1255 2023 172 901 17 153 185 445 Cuts at: 5476 325 Size: 1498 Fragments arranged by size: 2023 1498 1255 901 445 185 172 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3291 3925 4895 Size: 274 80 33 51 1443 1314 634 970 Cuts at: 4895 6607 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1314 970 634 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6609 6641 6609 Size: 32 6617 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6649 XbaI T'CTAG_A Cuts at: 2899 3985 2899 Size: 1086 5563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3468 3634 3468 Size: 166 6483 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3974 1870 Size: 2104 4545 XmnI GAAnn'nnTTC Cuts at: 2306 2536 3065 3533 4778 2306 Size: 230 529 468 1245 4177 Fragments arranged by size: 4177 1245 529 468 230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4457 276 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI