Restriction map for pFN21ASDA1540
 (Circular) (Six-base) MAPSORT of: pFN21ASDA1540.seq  Check: 9329  from: 1  to: 5998



pFN21ASDA1540  5998 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3044    3044 
   Size:       5998
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3808     278 
   Size:         53      83     186     936    2272    2468

  Fragments arranged by size: 

                2468    2272     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3341    1937 
   Size:       1404    4594
 
Acc65I G'GTAC_C

Cuts at:   3319    3319 
   Size:       5998
 
AclI AA'CG_TT

Cuts at:   1563    4125    4498    1563 
   Size:       2562     373    3063

  Fragments arranged by size: 

                3063    2562     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3061    3148    4061    5445    5713    1632 
   Size:       1429      87     913    1384     268    1917

  Fragments arranged by size: 

                1917    1429    1384     913     268      87
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5801
 
AflIII A'CryG_T

Cuts at:   1203    4897    5874    1203 
   Size:       3694     977    1327

  Fragments arranged by size: 

                3694    1327     977
 
AgeI A'CCGG_T

Cuts at:   4829    4829 
   Size:       5998
 
AhdI GACnn_n'nnGTC

Cuts at:   4727    4727 
   Size:       5998
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5998
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2880    1184 
   Size:       1696    4302
 
AlwNI CAG_nnn'CTG

Cuts at:   3036    3741    5313    3036 
   Size:        705    1572    3721

  Fragments arranged by size: 

                3721    1572     705
 
ApaLI G'TGCA_C

Cuts at:   4055    5211    4055 
   Size:       1156    4842
 
ApoI r'AATT_y

Cuts at:   1493    3307    3533    3685    5794    1493 
   Size:       1814     226     152    2109    1697

  Fragments arranged by size: 

                2109    1814    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4552     160 
   Size:        872    3520    1606

  Fragments arranged by size: 

                3520    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5998
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3323    1870 
   Size:         73    1380    4545

  Fragments arranged by size: 

                4545    1380      73
 
BamHI G'GATC_C

Cuts at:   3328    3695    3328 
   Size:        367    5631
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3319    4774     618 
   Size:        325     217     627    1532    1455    1842

  Fragments arranged by size: 

                1842    1532    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2079    3205    3317    1391 
   Size:        688    1126     112    4072

  Fragments arranged by size: 

                4072    1126     688     112
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2263    2894    3257     928 
   Size:        971     364     631     363    3669

  Fragments arranged by size: 

                3669     971     631     364     363
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4189    4223    1903 
   Size:         34    2252      34    3678

  Fragments arranged by size: 

                3678    2252      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3890    5100    3890 
   Size:       1210    4788
 
BclI T'GATC_A

Cuts at:   1548    2379    1548 
   Size:        831    5167
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4609     136 
   Size:        107     122      71     631     605    2937    1525

  Fragments arranged by size: 

                2937    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5993    5993 
   Size:       5998
 
BlpI GC'TnA_GC

Cuts at:   3415    3415 
   Size:       5998
 
Bme1580I G_kGCm'C

Cuts at:   1790    2130    4059    5215    1790 
   Size:        340    1929    1156    2573

  Fragments arranged by size: 

                2573    1929    1156     340
 
BmgBI CAC'GTC

Cuts at:   1318    2491    1318 
   Size:       1173    4825
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2467    3146    3729 
   Size:        416      53      83     399      24    1050     679     583

Cuts at:   3729    4687     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1050     958     679     583     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5998
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3224    4658    1239 
   Size:       1985    1434    2579

  Fragments arranged by size: 

                2579    1985    1434
 
Bpu10I CC'TnA_GC

Cuts at:   2709    2709 
   Size:       5998
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4123    4988    5286    4123 
   Size:        865     298    4835

  Fragments arranged by size: 

                4835     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4661     882 
   Size:        617    3162    2219

  Fragments arranged by size: 

                3162    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3295    3789     493 
   Size:        711    2091     494    2702

  Fragments arranged by size: 

                2702    2091     711     494
 
BsaBI GATnn'nnATC

Cuts at:   2567    5992    2567 
   Size:       3425    2573
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3805    4187    4822     275 
   Size:         53      83     186     936    2272     382     635    1451

  Fragments arranged by size: 

                2272    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4431    4829    5103    5250    5661    4431 
   Size:        398     274     147     411    4768

  Fragments arranged by size: 

                4768     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2153    2183    1178 
   Size:         30     165      30     750      30    4993

  Fragments arranged by size: 

                4993     750     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2042    2768    2951    1186 
   Size:        856     726     183    4233

  Fragments arranged by size: 

                4233     856     726     183
 
BseYI C'CCAG_C

Cuts at:   5201    5697    5201 
   Size:        496    5502
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2471    1163 
   Size:        573     735    4690

  Fragments arranged by size: 

                4690     735     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3448    4209    5237    1540 
   Size:        275     186    1447     761    1028    2301

  Fragments arranged by size: 

                2301    1447    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4059    4144    5215    4059 
   Size:         85    1071    4842

  Fragments arranged by size: 

                4842    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2331    3509    3602    2331 
   Size:       1178      93    4727

  Fragments arranged by size: 

                4727    1178      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2497    2497 
   Size:       5998
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2079    2130    3205    3317    4059    4144    5215 
   Size:        399     289      51    1075     112     742      85    1071

Cuts at:   5215    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1075    1071     742     399     289     112      85
                  51
 
BspEI T'CCGG_A

Cuts at:   5661    5661 
   Size:       5998
 
BspHI T'CATG_A

Cuts at:   3887    5617    3887 
   Size:       1730    4268
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3044    3353     844 
   Size:       2200     309    3489

  Fragments arranged by size: 

                3489    2200     309
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3885    1394 
   Size:        429    2062    3507

  Fragments arranged by size: 

                3507    2062     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2514    4493    4667      65 
   Size:       1183    1266    1979     174    1396

  Fragments arranged by size: 

                1979    1396    1266    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4642    4829    1700 
   Size:        110    2832     187    2869

  Fragments arranged by size: 

                2869    2832     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5998
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5998
 
BssSI C'ACGA_G

Cuts at:   2081    2802    4058    5070    2081 
   Size:        721    1256    1012    3009

  Fragments arranged by size: 

                3009    1256    1012     721
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3036     794 
   Size:       2242    3756
 
BstBI TT'CG_AA

Cuts at:   2007    4802    2007 
   Size:       2795    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5998
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2521    2521 
   Size:       5998
 
BstYI r'GATC_y

Cuts at:   1972    2562    3328    3695    4080    4097    5549    5635    5647 
   Size:        590     766     367     385      17    1452      86      12

Cuts at:   5647    5993    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     766     590     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3129    3129 
   Size:       5998
 
BtgI C'CryG_G

Cuts at:    513    1065    2022    2226    2607     513 
   Size:        552     957     204     381    3904

  Fragments arranged by size: 

                3904     957     552     381     204
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2575    2856     487 
   Size:        683      56    1349     281    3629

  Fragments arranged by size: 

                3629    1349     683     281      56
 
BtsI GCAGTG_nn'

Cuts at:   2483    3514    4308    4328    2483 
   Size:       1031     794      20    4153

  Fragments arranged by size: 

                4153    1031     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5998
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5963
 
DraI TTT'AAA

Cuts at:   3302    3302 
   Size:       5998
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3259    5005     809 
   Size:        507    1943    1746    1802

  Fragments arranged by size: 

                1943    1802    1746     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2121    2334    2745 
   Size:         54    1425      50     275      18     291     213     411

Cuts at:   2745    2808    3193    3445    4334    5963       8 
   Size:         63     385     252     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     411     385     291     275     252
                 213      63      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3445    1537 
   Size:        275    1633    4090

  Fragments arranged by size: 

                4090    1633     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2351    2729    3928    1353 
   Size:        998     378    1199    3423

  Fragments arranged by size: 

                3423    1199     998     378
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4585    4959    5105     387 
   Size:       1064     138    2996     374     146    1280

  Fragments arranged by size: 

                2996    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3061    3148    3224    4061    4658    5445    5713 
   Size:        393    1429      87      76     837     597     787     268

Cuts at:   5713    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1429     837     787     597     393     268      87
                  76
 
EcoRI G'AATT_C

Cuts at:   3307    3685    3307 
   Size:        378    5620
 
FspI TGC'GCA

Cuts at:   4504    4504 
   Size:       5998
 
HaeII r_GCGC'y

Cuts at:   1678    2203    2211    5145    1678 
   Size:        525       8    2934    2531

  Fragments arranged by size: 

                2934    2531     525       8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3094    3126    3248    3280    4639 
   Size:         32     135      32    1522      32     122      32    1359

Cuts at:   4639    4671    4713    4745    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1522    1359     135     122      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2356    3342    3588     669 
   Size:        988     281     418     986     246    3079

  Fragments arranged by size: 

                3079     988     986     418     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5998
 
HpaI GTT'AAC

Cuts at:   3588    3588 
   Size:       5998
 
KpnI G_GTAC'C

Cuts at:   3323    3323 
   Size:       5998
 
MfeI C'AATT_G

Cuts at:   3597    3597 
   Size:       5998
 
MluI A'CGCG_T

Cuts at:   5874    5874 
   Size:       5998
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2196    5112    5296    1305 
   Size:        891    2916     184    2007

  Fragments arranged by size: 

                2916    2007     891     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2747    2810    3195      10 
   Size:         54    1425     343     915      63     385    2813

  Fragments arranged by size: 

                2813    1425     915     385     343      63      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2519    2789    2897    3956    4315    4474     518 
   Size:        648    1353     270     108    1059     359     159    2042

  Fragments arranged by size: 

                2042    1353    1059     648     359     270     159     108
            
 
MspA1I CmG'CkG

Cuts at:   1606    2717    3414    4091    5239    5484    1606 
   Size:       1111     697     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1111     697     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2022    2607     513 
   Size:        552     957     585    3904

  Fragments arranged by size: 

                3904     957     585     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5998
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5998
 
NotI GC'GGCC_GC

Cuts at:   3445    3445 
   Size:       5998
 
NspI r_CATG'y

Cuts at:   1132    1229    2452    2636    3109    3356    4901    1132 
   Size:         97    1223     184     473     247    1545    2229

  Fragments arranged by size: 

                2229    1545    1223     473     247     184      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5998
 
PciI A'CATG_T

Cuts at:   4897    4897 
   Size:       5998
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5998
 
PmeI GTTT'AAAC

Cuts at:   3302    3302 
   Size:       5998
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4067    4099    5580    5612    4067 
   Size:         32    1481      32    4453

  Fragments arranged by size: 

                4453    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5998
 
PsiI TTA'TAA

Cuts at:   3568    3568 
   Size:       5998
 
PstI C_TGCA'G

Cuts at:    830    3350     830 
   Size:       2520    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5998
 
PvuII CAG'CTG

Cuts at:   2717    2717 
   Size:       5998
 
SalI G'TCGA_C

Cuts at:   1936    3340    1936 
   Size:       1404    4594
 
SbfI CC_TGCA'GG

Cuts at:   3350    3350 
   Size:       5998
 
ScaI AGT'ACT

Cuts at:   2477    2861    4246    5958    2477 
   Size:        384    1385    1712    2517

  Fragments arranged by size: 

                2517    1712    1385     384
 
SexAI A'CCwGG_T

Cuts at:   2532    2532 
   Size:       5998
 
SfcI C'TryA_G

Cuts at:    826    1045    3346    4481    5162    5353     826 
   Size:        219    2301    1135     681     191    1471

  Fragments arranged by size: 

                2301    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5998
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5998
 
SmaI CCC'GGG

Cuts at:   1872    3325    1872 
   Size:       1453    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4102    5003    5265     820 
   Size:        197     926    2159     901     262    1553

  Fragments arranged by size: 

                2159    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3295    3789     493 
   Size:       2802     494    2702

  Fragments arranged by size: 

                2802    2702     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5998
 
SphI G_CATG'C

Cuts at:   1132    3356    1132 
   Size:       2224    3774
 
SspI AAT'ATT

Cuts at:      5      52    3922       5 
   Size:         47    3870    2081

  Fragments arranged by size: 

                3870    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5998
 
StyI C'CwwG_G

Cuts at:    513    1065    2022    2058    2607    3437     513 
   Size:        552     957      36     549     830    3074

  Fragments arranged by size: 

                3074     957     830     552     549      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4025    4042    4195    4380    4825     325 
   Size:       1255    2445      17     153     185     445    1498

  Fragments arranged by size: 

                2445    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2475    2859    4244 
   Size:        274      80      33      51    1443     498     384    1385

Cuts at:   4244    5956      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1385     498     384     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5958    5990    5958 
   Size:         32    5966
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5998
 
XbaI T'CTAG_A

Cuts at:   3334    3334 
   Size:       5998
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3242    3242 
   Size:       5998
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5998
 
XmaI C'CCGG_G

Cuts at:   1870    3323    1870 
   Size:       1453    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4127    4127 
   Size:       5998
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3806     276 
   Size:         53      83     186     936    2272    2468

  Fragments arranged by size: 

                2468    2272     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI  BstZ17I   DraIII  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI
     SrfI     SwaI
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