(Circular) (Six-base) MAPSORT of: pFN21ASDA1540.seq Check: 9329 from: 1 to: 5998 pFN21ASDA1540 5998 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3044 3044 Size: 5998 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3808 278 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3341 1937 Size: 1404 4594 Acc65I G'GTAC_C Cuts at: 3319 3319 Size: 5998 AclI AA'CG_TT Cuts at: 1563 4125 4498 1563 Size: 2562 373 3063 Fragments arranged by size: 3063 2562 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3061 3148 4061 5445 5713 1632 Size: 1429 87 913 1384 268 1917 Fragments arranged by size: 1917 1429 1384 913 268 87 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5801 AflIII A'CryG_T Cuts at: 1203 4897 5874 1203 Size: 3694 977 1327 Fragments arranged by size: 3694 1327 977 AgeI A'CCGG_T Cuts at: 4829 4829 Size: 5998 AhdI GACnn_n'nnGTC Cuts at: 4727 4727 Size: 5998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2880 1184 Size: 1696 4302 AlwNI CAG_nnn'CTG Cuts at: 3036 3741 5313 3036 Size: 705 1572 3721 Fragments arranged by size: 3721 1572 705 ApaLI G'TGCA_C Cuts at: 4055 5211 4055 Size: 1156 4842 ApoI r'AATT_y Cuts at: 1493 3307 3533 3685 5794 1493 Size: 1814 226 152 2109 1697 Fragments arranged by size: 2109 1814 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4552 160 Size: 872 3520 1606 Fragments arranged by size: 3520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 AvaI C'yCGr_G Cuts at: 1870 1943 3323 1870 Size: 73 1380 4545 Fragments arranged by size: 4545 1380 73 BamHI G'GATC_C Cuts at: 3328 3695 3328 Size: 367 5631 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3319 4774 618 Size: 325 217 627 1532 1455 1842 Fragments arranged by size: 1842 1532 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2079 3205 3317 1391 Size: 688 1126 112 4072 Fragments arranged by size: 4072 1126 688 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2263 2894 3257 928 Size: 971 364 631 363 3669 Fragments arranged by size: 3669 971 631 364 363 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4189 4223 1903 Size: 34 2252 34 3678 Fragments arranged by size: 3678 2252 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3890 5100 3890 Size: 1210 4788 BclI T'GATC_A Cuts at: 1548 2379 1548 Size: 831 5167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4609 136 Size: 107 122 71 631 605 2937 1525 Fragments arranged by size: 2937 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5993 5993 Size: 5998 BlpI GC'TnA_GC Cuts at: 3415 3415 Size: 5998 Bme1580I G_kGCm'C Cuts at: 1790 2130 4059 5215 1790 Size: 340 1929 1156 2573 Fragments arranged by size: 2573 1929 1156 340 BmgBI CAC'GTC Cuts at: 1318 2491 1318 Size: 1173 4825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2467 3146 3729 Size: 416 53 83 399 24 1050 679 583 Cuts at: 3729 4687 442 Size: 958 1753 Fragments arranged by size: 1753 1050 958 679 583 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5998 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3224 4658 1239 Size: 1985 1434 2579 Fragments arranged by size: 2579 1985 1434 Bpu10I CC'TnA_GC Cuts at: 2709 2709 Size: 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4123 4988 5286 4123 Size: 865 298 4835 Fragments arranged by size: 4835 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4661 882 Size: 617 3162 2219 Fragments arranged by size: 3162 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3295 3789 493 Size: 711 2091 494 2702 Fragments arranged by size: 2702 2091 711 494 BsaBI GATnn'nnATC Cuts at: 2567 5992 2567 Size: 3425 2573 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3805 4187 4822 275 Size: 53 83 186 936 2272 382 635 1451 Fragments arranged by size: 2272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4431 4829 5103 5250 5661 4431 Size: 398 274 147 411 4768 Fragments arranged by size: 4768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4993 Fragments arranged by size: 4993 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2768 2951 1186 Size: 856 726 183 4233 Fragments arranged by size: 4233 856 726 183 BseYI C'CCAG_C Cuts at: 5201 5697 5201 Size: 496 5502 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 1163 Size: 573 735 4690 Fragments arranged by size: 4690 735 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3448 4209 5237 1540 Size: 275 186 1447 761 1028 2301 Fragments arranged by size: 2301 1447 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4059 4144 5215 4059 Size: 85 1071 4842 Fragments arranged by size: 4842 1071 85 BsmI GAATG_Cn' Cuts at: 2331 3509 3602 2331 Size: 1178 93 4727 Fragments arranged by size: 4727 1178 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2497 2497 Size: 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2130 3205 3317 4059 4144 5215 Size: 399 289 51 1075 112 742 85 1071 Cuts at: 5215 1391 Size: 2174 Fragments arranged by size: 2174 1075 1071 742 399 289 112 85 51 BspEI T'CCGG_A Cuts at: 5661 5661 Size: 5998 BspHI T'CATG_A Cuts at: 3887 5617 3887 Size: 1730 4268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 3353 844 Size: 2200 309 3489 Fragments arranged by size: 3489 2200 309 BsrBI CCG'CTC Cuts at: 1394 1823 3885 1394 Size: 429 2062 3507 Fragments arranged by size: 3507 2062 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2514 4493 4667 65 Size: 1183 1266 1979 174 1396 Fragments arranged by size: 1979 1396 1266 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4642 4829 1700 Size: 110 2832 187 2869 Fragments arranged by size: 2869 2832 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5998 BssSI C'ACGA_G Cuts at: 2081 2802 4058 5070 2081 Size: 721 1256 1012 3009 Fragments arranged by size: 3009 1256 1012 721 BstAPI GCAn_nnn'nTGC Cuts at: 794 3036 794 Size: 2242 3756 BstBI TT'CG_AA Cuts at: 2007 4802 2007 Size: 2795 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5998 BstXI CCAn_nnnn'nTGG Cuts at: 2521 2521 Size: 5998 BstYI r'GATC_y Cuts at: 1972 2562 3328 3695 4080 4097 5549 5635 5647 Size: 590 766 367 385 17 1452 86 12 Cuts at: 5647 5993 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 766 590 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3129 3129 Size: 5998 BtgI C'CryG_G Cuts at: 513 1065 2022 2226 2607 513 Size: 552 957 204 381 3904 Fragments arranged by size: 3904 957 552 381 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2575 2856 487 Size: 683 56 1349 281 3629 Fragments arranged by size: 3629 1349 683 281 56 BtsI GCAGTG_nn' Cuts at: 2483 3514 4308 4328 2483 Size: 1031 794 20 4153 Fragments arranged by size: 4153 1031 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5963 DraI TTT'AAA Cuts at: 3302 3302 Size: 5998 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3259 5005 809 Size: 507 1943 1746 1802 Fragments arranged by size: 1943 1802 1746 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2334 2745 Size: 54 1425 50 275 18 291 213 411 Cuts at: 2745 2808 3193 3445 4334 5963 8 Size: 63 385 252 889 1629 43 Fragments arranged by size: 1629 1425 889 411 385 291 275 252 213 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3445 1537 Size: 275 1633 4090 Fragments arranged by size: 4090 1633 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 2729 3928 1353 Size: 998 378 1199 3423 Fragments arranged by size: 3423 1199 998 378 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4585 4959 5105 387 Size: 1064 138 2996 374 146 1280 Fragments arranged by size: 2996 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3061 3148 3224 4061 4658 5445 5713 Size: 393 1429 87 76 837 597 787 268 Cuts at: 5713 1239 Size: 1524 Fragments arranged by size: 1524 1429 837 787 597 393 268 87 76 EcoRI G'AATT_C Cuts at: 3307 3685 3307 Size: 378 5620 FspI TGC'GCA Cuts at: 4504 4504 Size: 5998 HaeII r_GCGC'y Cuts at: 1678 2203 2211 5145 1678 Size: 525 8 2934 2531 Fragments arranged by size: 2934 2531 525 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3094 3126 3248 3280 4639 Size: 32 135 32 1522 32 122 32 1359 Cuts at: 4639 4671 4713 4745 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1522 1359 135 122 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2356 3342 3588 669 Size: 988 281 418 986 246 3079 Fragments arranged by size: 3079 988 986 418 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5998 HpaI GTT'AAC Cuts at: 3588 3588 Size: 5998 KpnI G_GTAC'C Cuts at: 3323 3323 Size: 5998 MfeI C'AATT_G Cuts at: 3597 3597 Size: 5998 MluI A'CGCG_T Cuts at: 5874 5874 Size: 5998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 5112 5296 1305 Size: 891 2916 184 2007 Fragments arranged by size: 2916 2007 891 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 2810 3195 10 Size: 54 1425 343 915 63 385 2813 Fragments arranged by size: 2813 1425 915 385 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 2789 2897 3956 4315 4474 518 Size: 648 1353 270 108 1059 359 159 2042 Fragments arranged by size: 2042 1353 1059 648 359 270 159 108 MspA1I CmG'CkG Cuts at: 1606 2717 3414 4091 5239 5484 1606 Size: 1111 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 1111 697 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2607 513 Size: 552 957 585 3904 Fragments arranged by size: 3904 957 585 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5998 NotI GC'GGCC_GC Cuts at: 3445 3445 Size: 5998 NspI r_CATG'y Cuts at: 1132 1229 2452 2636 3109 3356 4901 1132 Size: 97 1223 184 473 247 1545 2229 Fragments arranged by size: 2229 1545 1223 473 247 184 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5998 PciI A'CATG_T Cuts at: 4897 4897 Size: 5998 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5998 PmeI GTTT'AAAC Cuts at: 3302 3302 Size: 5998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4099 5580 5612 4067 Size: 32 1481 32 4453 Fragments arranged by size: 4453 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5998 PsiI TTA'TAA Cuts at: 3568 3568 Size: 5998 PstI C_TGCA'G Cuts at: 830 3350 830 Size: 2520 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5998 PvuII CAG'CTG Cuts at: 2717 2717 Size: 5998 SalI G'TCGA_C Cuts at: 1936 3340 1936 Size: 1404 4594 SbfI CC_TGCA'GG Cuts at: 3350 3350 Size: 5998 ScaI AGT'ACT Cuts at: 2477 2861 4246 5958 2477 Size: 384 1385 1712 2517 Fragments arranged by size: 2517 1712 1385 384 SexAI A'CCwGG_T Cuts at: 2532 2532 Size: 5998 SfcI C'TryA_G Cuts at: 826 1045 3346 4481 5162 5353 826 Size: 219 2301 1135 681 191 1471 Fragments arranged by size: 2301 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5998 SmaI CCC'GGG Cuts at: 1872 3325 1872 Size: 1453 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4102 5003 5265 820 Size: 197 926 2159 901 262 1553 Fragments arranged by size: 2159 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3295 3789 493 Size: 2802 494 2702 Fragments arranged by size: 2802 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5998 SphI G_CATG'C Cuts at: 1132 3356 1132 Size: 2224 3774 SspI AAT'ATT Cuts at: 5 52 3922 5 Size: 47 3870 2081 Fragments arranged by size: 3870 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5998 StyI C'CwwG_G Cuts at: 513 1065 2022 2058 2607 3437 513 Size: 552 957 36 549 830 3074 Fragments arranged by size: 3074 957 830 552 549 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4025 4042 4195 4380 4825 325 Size: 1255 2445 17 153 185 445 1498 Fragments arranged by size: 2445 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 2859 4244 Size: 274 80 33 51 1443 498 384 1385 Cuts at: 4244 5956 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1385 498 384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5958 5990 5958 Size: 32 5966 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5998 XbaI T'CTAG_A Cuts at: 3334 3334 Size: 5998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3242 3242 Size: 5998 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5998 XmaI C'CCGG_G Cuts at: 1870 3323 1870 Size: 1453 4545 XmnI GAAnn'nnTTC Cuts at: 4127 4127 Size: 5998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3806 276 Size: 53 83 186 936 2272 2468 Fragments arranged by size: 2468 2272 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI