(Circular) (Six-base) MAPSORT of: pFN21ASDA1499.seq Check: 5630 from: 1 to: 6580 pFN21ASDA1499 6580 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3799 3799 Size: 6580 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2297 4390 278 Size: 53 83 186 936 761 2093 2468 Fragments arranged by size: 2468 2093 936 761 186 83 53 AccI GT'mk_AC Cuts at: 1937 3309 3923 1937 Size: 1372 614 4594 Fragments arranged by size: 4594 1372 614 Acc65I G'GTAC_C Cuts at: 2990 3174 3901 2990 Size: 184 727 5669 Fragments arranged by size: 5669 727 184 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2847 2922 4643 6027 6295 1632 Size: 1215 75 1721 1384 268 1917 Fragments arranged by size: 1917 1721 1384 1215 268 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6383 AflIII A'CryG_T Cuts at: 1203 2505 3081 5479 6456 1203 Size: 1302 576 2398 977 1327 Fragments arranged by size: 2398 1327 1302 977 576 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3617 3813 1184 Size: 2433 196 3951 Fragments arranged by size: 3951 2433 196 AlwNI CAG_nnn'CTG Cuts at: 4323 5895 4323 Size: 1572 5008 ApaI G_GGCC'C Cuts at: 2277 2277 Size: 6580 ApaLI G'TGCA_C Cuts at: 2438 4637 5793 2438 Size: 2199 1156 3225 Fragments arranged by size: 3225 2199 1156 ApoI r'AATT_y Cuts at: 1493 3889 4115 4267 6376 1493 Size: 2396 226 152 2109 1697 Fragments arranged by size: 2396 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5134 160 Size: 872 4102 1606 Fragments arranged by size: 4102 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2260 2486 3905 1870 Size: 73 317 226 1419 4545 Fragments arranged by size: 4545 1419 317 226 73 BamHI G'GATC_C Cuts at: 2890 3113 3397 3910 4277 2890 Size: 223 284 513 367 5193 Fragments arranged by size: 5193 513 367 284 223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2610 2990 3174 3717 3775 Size: 325 217 627 823 380 184 543 58 Cuts at: 3775 3811 3901 5356 618 Size: 36 90 1455 1842 Fragments arranged by size: 1842 1455 823 627 543 380 325 217 184 90 58 36 BanII G_rGCy'C Cuts at: 1391 2277 3639 3899 1391 Size: 886 1362 260 4072 Fragments arranged by size: 4072 1362 886 260 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5609 BbvCI CC'TCA_GC Cuts at: 2363 2363 Size: 6580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2900 2934 4771 4805 1903 Size: 34 963 34 1837 34 3678 Fragments arranged by size: 3678 1837 963 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3520 4472 5682 3520 Size: 952 1210 4418 Fragments arranged by size: 4418 1210 952 BclI T'GATC_A Cuts at: 1548 2353 1548 Size: 805 5775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2418 5191 136 Size: 107 122 71 631 605 746 2773 1525 Fragments arranged by size: 2773 1525 746 631 605 122 107 71 BglII A'GATC_T Cuts at: 6575 6575 Size: 6580 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2277 2442 2613 3780 4641 5797 1790 Size: 487 165 171 1167 861 1156 2573 Fragments arranged by size: 2573 1167 1156 861 487 171 165 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4311 5269 442 Size: 416 53 83 399 24 2894 958 1753 Fragments arranged by size: 2894 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2559 2591 2764 3081 3629 5240 1239 Size: 1320 32 173 317 548 1611 2579 Fragments arranged by size: 2579 1611 1320 548 317 173 32 Bpu10I CC'TnA_GC Cuts at: 2363 2363 Size: 6580 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 4705 5570 5868 2906 Size: 1799 865 298 3618 Fragments arranged by size: 3618 1799 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2391 5243 882 Size: 617 892 2852 2219 Fragments arranged by size: 2852 2219 892 617 BsaAI yAC'GTr Cuts at: 493 1204 3283 3860 3877 4371 493 Size: 711 2079 577 17 494 2702 Fragments arranged by size: 2702 2079 711 577 494 17 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2294 3237 4387 4769 Size: 53 83 186 936 761 943 1150 382 Cuts at: 4769 5404 275 Size: 635 1451 Fragments arranged by size: 1451 1150 943 936 761 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2180 2526 3870 5013 5411 5685 5832 6243 2180 Size: 346 1344 1143 398 274 147 411 2517 Fragments arranged by size: 2517 1344 1143 411 398 346 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3829 3859 1178 Size: 30 165 30 2426 30 3899 Fragments arranged by size: 3899 2426 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2188 3647 1186 Size: 1002 1459 4119 Fragments arranged by size: 4119 1459 1002 BseYI C'CCAG_C Cuts at: 2270 3333 3728 3793 5783 6279 2270 Size: 1063 395 65 1990 496 2571 Fragments arranged by size: 2571 1990 1063 496 395 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3709 3737 1163 Size: 573 1973 28 4006 Fragments arranged by size: 4006 1973 573 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2442 2799 3639 4641 4726 5797 2442 Size: 357 840 1002 85 1071 3225 Fragments arranged by size: 3225 1071 1002 840 357 85 BsmI GAATG_Cn' Cuts at: 4091 4184 4091 Size: 93 6487 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 2974 2285 Size: 689 5891 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2442 2613 2799 3639 3780 3899 Size: 399 487 165 171 186 840 141 119 Cuts at: 3899 4641 4726 5797 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 840 742 487 399 186 171 165 141 119 85 BspEI T'CCGG_A Cuts at: 2526 6243 2526 Size: 3717 2863 BspHI T'CATG_A Cuts at: 4469 6199 4469 Size: 1730 4850 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3799 3935 844 Size: 2955 136 3489 Fragments arranged by size: 3489 2955 136 BsrBI CCG'CTC Cuts at: 1394 1823 4467 1394 Size: 429 2644 3507 Fragments arranged by size: 3507 2644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3828 5075 5249 65 Size: 1183 2580 1247 174 1396 Fragments arranged by size: 2580 1396 1247 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3087 3769 5224 5411 1700 Size: 110 1277 682 1455 187 2869 Fragments arranged by size: 2869 1455 1277 682 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 4640 5652 4640 Size: 1012 5568 BstAPI GCAn_nnn'nTGC Cuts at: 794 3283 794 Size: 2489 4091 BstBI TT'CG_AA Cuts at: 2007 5384 2007 Size: 3377 3203 BstEII G'GTnAC_C Cuts at: 1184 3562 1184 Size: 2378 4202 BstXI CCAn_nnnn'nTGG Cuts at: 2147 2338 2768 3860 2147 Size: 191 430 1092 4867 Fragments arranged by size: 4867 1092 430 191 BstYI r'GATC_y Cuts at: 1972 2890 3113 3397 3910 4277 4662 4679 6131 Size: 918 223 284 513 367 385 17 1452 Cuts at: 6131 6217 6229 6575 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 918 513 385 367 346 284 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2416 2618 3723 513 Size: 552 957 394 202 1105 3370 Fragments arranged by size: 3370 1105 957 552 394 202 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3048 3138 3277 3671 3697 3764 4096 4890 Size: 608 90 139 394 26 67 332 794 Cuts at: 4890 4910 2440 Size: 20 4110 Fragments arranged by size: 4110 794 608 394 332 139 90 67 26 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6545 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DraIII CAC_nnn'GTG Cuts at: 3724 3724 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3158 5587 809 Size: 507 1842 2429 1802 Fragments arranged by size: 2429 1842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2621 3486 Size: 54 1425 50 275 18 195 596 865 Cuts at: 3486 4027 4916 6545 8 Size: 541 889 1629 43 Fragments arranged by size: 1629 1425 889 865 596 541 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3033 3647 4510 1353 Size: 1680 614 863 3423 Fragments arranged by size: 3423 1680 863 614 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3637 3637 Size: 6580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2559 2591 2764 2847 2922 3081 3629 Size: 393 927 32 173 83 75 159 548 Cuts at: 3629 4643 5240 6027 6295 1239 Size: 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 927 787 597 548 393 268 173 159 83 75 32 EcoNI CCTnn'n_nnAGG Cuts at: 2939 2939 Size: 6580 EcoO109I rG'GnC_Cy Cuts at: 2274 2274 Size: 6580 EcoRI G'AATT_C Cuts at: 3889 4267 3889 Size: 378 6202 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 2328 2658 5727 1678 Size: 650 330 3069 2531 Fragments arranged by size: 3069 2531 650 330 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2954 2986 3518 3550 3829 Size: 32 135 32 1382 32 532 32 279 Cuts at: 3829 3861 5221 5253 5295 5327 1373 Size: 32 1360 32 42 32 2626 Fragments arranged by size: 2626 1382 1360 532 279 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3924 4170 669 Size: 988 281 1986 246 3079 Fragments arranged by size: 3079 1986 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 2994 3178 3905 2994 Size: 184 727 5669 Fragments arranged by size: 5669 727 184 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 5694 5878 1305 Size: 921 3468 184 2007 Fragments arranged by size: 3468 2007 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3488 10 Size: 54 1425 343 1656 3102 Fragments arranged by size: 3102 1656 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2407 4538 4897 5056 518 Size: 648 1241 2131 359 159 2042 Fragments arranged by size: 2131 2042 1241 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2583 3337 3685 3797 3996 4673 5821 6066 Size: 977 754 348 112 199 677 1148 245 Cuts at: 6066 1606 Size: 2120 Fragments arranged by size: 2120 1148 977 754 677 348 245 199 112 NaeI GCC'GGC Cuts at: 3089 3089 Size: 6580 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5071 Fragments arranged by size: 5071 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NgoMIV G'CCGG_C Cuts at: 3087 3087 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 2509 2690 3085 3938 5483 1132 Size: 97 1280 181 395 853 1545 2229 Fragments arranged by size: 2229 1545 1280 853 395 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 2505 3081 5479 2505 Size: 576 2398 3606 Fragments arranged by size: 3606 2398 576 PflMI CCAn_nnn'nTGG Cuts at: 1108 3484 1108 Size: 2376 4204 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PmlI CAC'GTG Cuts at: 3860 3860 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6580 PsiI TTA'TAA Cuts at: 3300 4150 3300 Size: 850 5730 PspOMI G'GGCC_C Cuts at: 2273 2273 Size: 6580 PspXI vC'TCGA_Gb Cuts at: 2260 2260 Size: 6580 PstI C_TGCA'G Cuts at: 830 3055 3133 3588 3932 830 Size: 2225 78 455 344 3478 Fragments arranged by size: 3478 2225 455 344 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 2583 3337 3685 3797 2583 Size: 754 348 112 5366 Fragments arranged by size: 5366 754 348 112 SacI G_AGCT'C Cuts at: 3639 3639 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SexAI A'CCwGG_T Cuts at: 3177 3177 Size: 6580 SfcI C'TryA_G Cuts at: 826 1045 3001 3051 3129 3138 3584 3928 5063 Size: 219 1956 50 78 9 446 344 1135 Cuts at: 5063 5744 5935 826 Size: 681 191 1471 Fragments arranged by size: 1956 1471 1135 681 446 344 219 191 78 50 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2260 2486 2885 4684 5585 5847 Size: 197 926 317 226 399 1799 901 262 Cuts at: 5847 820 Size: 1553 Fragments arranged by size: 1799 1553 926 901 399 317 262 226 197 SnaBI TAC'GTA Cuts at: 493 3877 4371 493 Size: 3384 494 2702 Fragments arranged by size: 3384 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 4504 5 Size: 47 4452 2081 Fragments arranged by size: 4452 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6580 StyI C'CwwG_G Cuts at: 513 1065 2022 2812 3258 3393 4019 513 Size: 552 957 790 446 135 626 3074 Fragments arranged by size: 3074 957 790 626 552 446 135 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3347 3449 3807 4607 4624 4777 4962 Size: 1255 1767 102 358 800 17 153 185 Cuts at: 4962 5407 325 Size: 445 1498 Fragments arranged by size: 1767 1498 1255 800 445 358 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2636 2679 4826 Size: 274 80 33 51 1443 659 43 2147 Cuts at: 4826 6538 96 Size: 1712 138 Fragments arranged by size: 2147 1712 1443 659 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3459 3491 6540 6572 3459 Size: 32 3049 32 3467 Fragments arranged by size: 3467 3049 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2966 1316 Size: 1650 4930 XbaI T'CTAG_A Cuts at: 2452 3916 2452 Size: 1464 5116 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2057 3193 3801 2057 Size: 1136 608 4836 Fragments arranged by size: 4836 1136 608 XhoI C'TCGA_G Cuts at: 1943 2260 2486 1943 Size: 317 226 6037 Fragments arranged by size: 6037 317 226 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 4709 4709 Size: 6580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2295 4388 276 Size: 53 83 186 936 761 2093 2468 Fragments arranged by size: 2468 2093 936 761 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI