Restriction map for pFN21ASDA1499
 (Linear) (Six-base) MAPSORT of: KIAA1499.seq  Check: 1074  from: 1  to: 1851



KIAA1499.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1776    1851 
   Size:       1776      75
 
AatII G_ACGT'C

Cuts at:      0     274    1851 
   Size:        274    1577
 
AccI GT'mk_AC

Cuts at:      0    1286    1851 
   Size:       1286     565
 
Acc65I G'GTAC_C

Cuts at:      0     967    1151    1851 
   Size:        967     184     700

  Fragments arranged by size: 

                 967     700     184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     824     899    1851 
   Size:        824      75     952

  Fragments arranged by size: 

                 952     824      75
 
AflIII A'CryG_T

Cuts at:      0     482    1058    1851 
   Size:        482     576     793

  Fragments arranged by size: 

                 793     576     482
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1594    1790    1851 
   Size:       1594     196      61

  Fragments arranged by size: 

                1594     196      61
 
ApaI G_GGCC'C

Cuts at:      0     254    1851 
   Size:        254    1597
 
ApaLI G'TGCA_C

Cuts at:      0     415    1851 
   Size:        415    1436
 
AvaI C'yCGr_G

Cuts at:      0     237     463    1851 
   Size:        237     226    1388

  Fragments arranged by size: 

                1388     237     226
 
BamHI G'GATC_C

Cuts at:      0     867    1090    1374    1851 
   Size:        867     223     284     477

  Fragments arranged by size: 

                 867     477     284     223
 
BanI G'GyrC_C

Cuts at:      0     587     967    1151    1694    1752    1788    1851 
   Size:        587     380     184     543      58      36      63

  Fragments arranged by size: 

                 587     543     380     184      63      58      36
 
BanII G_rGCy'C

Cuts at:      0     254    1616    1851 
   Size:        254    1362     235

  Fragments arranged by size: 

                1362     254     235
 
BbvCI CC'TCA_GC

Cuts at:      0     340    1851 
   Size:        340    1511
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     877     911    1851 
   Size:        877      34     940

  Fragments arranged by size: 

                 940     877      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1497    1851 
   Size:       1497     354
 
BclI T'GATC_A

Cuts at:      0     330    1851 
   Size:        330    1521
 
BglI GCCn_nnn'nGGC

Cuts at:      0     395    1851 
   Size:        395    1456
 
Bme1580I G_kGCm'C

Cuts at:      0     254     419     590    1757    1851 
   Size:        254     165     171    1167      94

  Fragments arranged by size: 

                1167     254     171     165      94
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     568     741    1058    1606    1851 
   Size:        536      32     173     317     548     245

  Fragments arranged by size: 

                 548     536     317     245     173      32
 
Bpu10I CC'TnA_GC

Cuts at:      0     340    1851 
   Size:        340    1511
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     883    1851 
   Size:        883     968
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     368    1851 
   Size:        368    1483
 
BsaAI yAC'GTr

Cuts at:      0    1260    1837    1851 
   Size:       1260     577      14

  Fragments arranged by size: 

                1260     577      14
 
BsaHI Gr'CG_yC

Cuts at:      0     271    1214    1851 
   Size:        271     943     637

  Fragments arranged by size: 

                 943     637     271
 
BsaWI w'CCGG_w

Cuts at:      0     157     503    1851 
   Size:        157     346    1348

  Fragments arranged by size: 

                1348     346     157
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1806    1836    1851 
   Size:       1806      30      15

  Fragments arranged by size: 

                1806      30      15
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     165    1624    1851 
   Size:        165    1459     227

  Fragments arranged by size: 

                1459     227     165
 
BseYI C'CCAG_C

Cuts at:      0     247    1310    1705    1770    1851 
   Size:        247    1063     395      65      81

  Fragments arranged by size: 

                1063     395     247      81      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1686    1714    1851 
   Size:       1686      28     137

  Fragments arranged by size: 

                1686     137      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     419     776    1616    1851 
   Size:        419     357     840     235

  Fragments arranged by size: 

                 840     419     357     235
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     262     951    1851 
   Size:        262     689     900

  Fragments arranged by size: 

                 900     689     262
 
Bsp1286I G_dGCh'C

Cuts at:      0     254     419     590     776    1616    1757    1851 
   Size:        254     165     171     186     840     141      94

  Fragments arranged by size: 

                 840     254     186     171     165     141      94
 
BspEI T'CCGG_A

Cuts at:      0     503    1851 
   Size:        503    1348
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1776    1851 
   Size:       1776      75
 
BsrDI GCAATG_nn'

Cuts at:      0    1805    1851 
   Size:       1805      46
 
BsrFI r'CCGG_y

Cuts at:      0    1064    1746    1851 
   Size:       1064     682     105

  Fragments arranged by size: 

                1064     682     105
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1260    1851 
   Size:       1260     591
 
BstEII G'GTnAC_C

Cuts at:      0    1539    1851 
   Size:       1539     312
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     124     315     745    1837    1851 
   Size:        124     191     430    1092      14

  Fragments arranged by size: 

                1092     430     191     124      14
 
BstYI r'GATC_y

Cuts at:      0     867    1090    1374    1851 
   Size:        867     223     284     477

  Fragments arranged by size: 

                 867     477     284     223
 
BtgI C'CryG_G

Cuts at:      0     393     595    1700    1851 
   Size:        393     202    1105     151

  Fragments arranged by size: 

                1105     393     202     151
 
BtsI GCAGTG_nn'

Cuts at:      0     417    1025    1115    1254    1648    1674    1741    1851 
   Size:        417     608      90     139     394      26      67     110

  Fragments arranged by size: 

                 608     417     394     139     110      90      67      26
            
 
DraIII CAC_nnn'GTG

Cuts at:      0    1701    1851 
   Size:       1701     150
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1135    1851 
   Size:       1135     716
 
EaeI y'GGCC_r

Cuts at:      0       2     598    1463    1851 
   Size:          2     596     865     388

  Fragments arranged by size: 

                 865     596     388       2
 
EarI CTCTTCn'nnn_

Cuts at:      0    1010    1624    1851 
   Size:       1010     614     227

  Fragments arranged by size: 

                1010     614     227
 
EcoICRI GAG'CTC

Cuts at:      0    1614    1851 
   Size:       1614     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     568     741     824     899    1058    1606    1851 
   Size:        536      32     173      83      75     159     548     245

  Fragments arranged by size: 

                 548     536     245     173     159      83      75      32
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     916    1851 
   Size:        916     935
 
EcoO109I rG'GnC_Cy

Cuts at:      0     251    1851 
   Size:        251    1600
 
HaeII r_GCGC'y

Cuts at:      0     305     635    1851 
   Size:        305     330    1216

  Fragments arranged by size: 

                1216     330     305
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     931     963    1495    1527    1806    1838    1851 
   Size:        931      32     532      32     279      32      13

  Fragments arranged by size: 

                 931     532     279      32      32      32      13
 
KpnI G_GTAC'C

Cuts at:      0     971    1155    1851 
   Size:        971     184     696

  Fragments arranged by size: 

                 971     696     184
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1851 
   Size:        203    1648
 
MscI TGG'CCA

Cuts at:      0    1465    1851 
   Size:       1465     386
 
MslI CAynn'nnrTG

Cuts at:      0     384    1851 
   Size:        384    1467
 
MspA1I CmG'CkG

Cuts at:      0     560    1314    1662    1774    1851 
   Size:        560     754     348     112      77

  Fragments arranged by size: 

                 754     560     348     112      77
 
NaeI GCC'GGC

Cuts at:      0    1066    1851 
   Size:       1066     785
 
NgoMIV G'CCGG_C

Cuts at:      0    1064    1851 
   Size:       1064     787
 
NspI r_CATG'y

Cuts at:      0     486     667    1062    1851 
   Size:        486     181     395     789

  Fragments arranged by size: 

                 789     486     395     181
 
PciI A'CATG_T

Cuts at:      0     482    1058    1851 
   Size:        482     576     793

  Fragments arranged by size: 

                 793     576     482
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1461    1851 
   Size:       1461     390
 
PmlI CAC'GTG

Cuts at:      0    1837    1851 
   Size:       1837      14
 
PsiI TTA'TAA

Cuts at:      0    1277    1851 
   Size:       1277     574
 
PspOMI G'GGCC_C

Cuts at:      0     250    1851 
   Size:        250    1601
 
PspXI vC'TCGA_Gb

Cuts at:      0     237    1851 
   Size:        237    1614
 
PstI C_TGCA'G

Cuts at:      0    1032    1110    1565    1851 
   Size:       1032      78     455     286

  Fragments arranged by size: 

                1032     455     286      78
 
PvuII CAG'CTG

Cuts at:      0     560    1314    1662    1774    1851 
   Size:        560     754     348     112      77

  Fragments arranged by size: 

                 754     560     348     112      77
 
SacI G_AGCT'C

Cuts at:      0    1616    1851 
   Size:       1616     235
 
SexAI A'CCwGG_T

Cuts at:      0    1154    1851 
   Size:       1154     697
 
SfcI C'TryA_G

Cuts at:      0     978    1028    1106    1115    1561    1851 
   Size:        978      50      78       9     446     290

  Fragments arranged by size: 

                 978     446     290      78      50       9
 
SmlI C'TyrA_G

Cuts at:      0     237     463     862    1851 
   Size:        237     226     399     989

  Fragments arranged by size: 

                 989     399     237     226
 
StyI C'CwwG_G

Cuts at:      0     789    1235    1370    1851 
   Size:        789     446     135     481

  Fragments arranged by size: 

                 789     481     446     135
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1324    1426    1784    1851 
   Size:       1324     102     358      67

  Fragments arranged by size: 

                1324     358     102      67
 
TatI w'GTAC_w

Cuts at:      0     613     656    1851 
   Size:        613      43    1195

  Fragments arranged by size: 

                1195     613      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1436    1468    1851 
   Size:       1436      32     383

  Fragments arranged by size: 

                1436     383      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     943    1851 
   Size:        943     908
 
XbaI T'CTAG_A

Cuts at:      0     429    1851 
   Size:        429    1422
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      34    1170    1778    1851 
   Size:         34    1136     608      73

  Fragments arranged by size: 

                1136     608      73      34
 
XhoI C'TCGA_G

Cuts at:      0     237     463    1851 
   Size:        237     226    1388

  Fragments arranged by size: 

                1388     237     226
 
ZraI GAC'GTC

Cuts at:      0     272    1851 
   Size:        272    1579
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI   AflIII     AlfI     ApaI
    ApaLI     AvaI    BamHI     BanI    BanII    BbvCI     BcgI    BciVI
     BclI     BglI Bme1580I     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BspEI    BspMI    BsrDI    BsrFI   BstAPI   BstEII    BstXI
    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI    PflMI     PmlI
     PsiI   PspOMI    PspXI     PstI    PvuII     SacI    SexAI     SfcI
     SmlI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII     AgeI     AhdI     AleI     AloI    AlwNI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BsaBI
    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrGI   BssHII    BssSI
    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI
     EciI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     XmaI     XmnI
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