Restriction map for
pFN21ASDA1427
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbvCI BciVI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MamI MfeI MflI
MluI MroI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 309 GTCTAC GT^[AC][GT]AC
AcyI 31 GGCGCC G[GA]^CG[CT]C
Asp700I 205 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 31 GGCGCC GGCGC^C
BbsI 831 GAAGAC GAAGAC(2/6)
BcgI 1002 GCACCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1076 TGATCA T^GATCA
BfaI 805 CTAG C^TAG
BlpI 1042 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1042 GCTAAGC GC^T[ACGT]AGC
BpuAI 831 GAAGAC GAAGAC(2/6)
BsaHI 31 GGCGCC G[GA]^CG[CT]C
BsaI 436 GAGACC GGTCTC(1/5)
BsiEI 738 CGACCG CG[GA][CT]^CG
BstAPI 1263 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1042 GCTAAGC GC^T[ACGT]AGC
DraIII 754 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 574 GACTGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1076 TGATCA T^GATCA
HaeII 31 GGCGCC [GA]GCGC^[CT]
HgaI 717 GACGC GACGC(5/10)
Hin1I 31 GGCGCC G[GA]^CG[CT]C
KasI 31 GGCGCC G^GCGCC
KspI 121 CCGCGG CCGC^GG
MaeI 805 CTAG C^TAG
MaeII 581 ACGT A^CGT
NaeI 1244 GCCGGC GCC^GGC
NarI 31 GGCGCC GG^CGCC
NgoMIV 1244 GCCGGC G^CCGGC
NspI 95 ACATGC [GA]CATG^[CT]
PleI 531 GACTC GAGTC(4/5)
SacII 121 CCGCGG CCGC^GG
SfoI 31 GGCGCC GGC^GCC
StuI 1109 AGGCCT AGG^CCT
TfiI 1147 GATTC G^A[AT]TC
Tsp509I 521 AATT ^AATT
XmnI 205 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 857 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1212 CAGCCACTG
AvaI 166 CTCGGG C^[CT]CG[GA]G
1197 CTCGGG
BalI 596 TGGCCA TGG^CCA
1170 TGGCCA
BcnI 764 CCGGG CC^[GC]GG
894 CCCGG
BsmAI 436 GAGAC GTCTC(1/5)
979 GTCTC
BsmFI 125 GGGAC GGGAC(10/14)
790 GGGAC
BsoBI 166 CTCGGG C^[CT]CG[GA]G
1197 CTCGGG
BsrFI 607 ACCGGC [GA]^CCGG[CT]
1244 GCCGGC
BstEII 75 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
BstPI 75 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
Cfr10I 607 ACCGGC [GA]^CCGG[CT]
1244 GCCGGC
DsaI 121 CCGCGG C^C[GA][CT]GG
200 CCACGG
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
990 GGTGACC
EcoT14I 585 CCAAGG C^C[AT][AT]GG
956 CCAAGG
FauI 71 GCGGG CCCGC(4/6)
123 GCGGG
HinfI 531 GACTC G^A[ACGT]TC
1147 GATTC
HphI 990 GGTGA GGTGA(8/7)
77 TCACC
MluNI 596 TGGCCA TGG^CCA
1170 TGGCCA
MscI 596 TGGCCA TGG^CCA
1170 TGGCCA
MseI 191 TTAA T^TAA
926 TTAA
NciI 764 CCGGG CC^[GC]GG
894 CCCGG
SfaNI 1227 GATGC GCATC(5/9)
56 GCATC
StyI 585 CCAAGG C^C[AT][AT]GG
956 CCAAGG
Tru9I 191 TTAA T^TAA
926 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AspHI AvaII
BanI BanII BbvI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PpuMI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfcI SmlI TseI Tsp45I TspRI
XcmI