Restriction map for
pFN21ASDA1427
(Linear) (Six-base) MAPSORT of: KIAA1427.seq Check: 6285 from: 1 to: 1278
KIAA1427.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 88 339 1278
Size: 88 251 939
Fragments arranged by size:
939 251 88
AccI GT'mk_AC
Cuts at: 0 310 1278
Size: 310 968
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 821 850 1039 1278
Size: 821 29 189 239
Fragments arranged by size:
821 239 189 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1178 1278
Size: 1178 100
AlwNI CAG_nnn'CTG
Cuts at: 0 862 1217 1278
Size: 862 355 61
Fragments arranged by size:
862 355 61
AvaI C'yCGr_G
Cuts at: 0 166 1197 1278
Size: 166 1031 81
Fragments arranged by size:
1031 166 81
BanI G'GyrC_C
Cuts at: 0 12 31 1262 1278
Size: 12 19 1231 16
Fragments arranged by size:
1231 19 16 12
BanII G_rGCy'C
Cuts at: 0 323 701 773 965 1204 1278
Size: 323 378 72 192 239 74
Fragments arranged by size:
378 323 239 192 74 72
BbeI G_GCGC'C
Cuts at: 0 35 1278
Size: 35 1243
BbsI GAAGACnn'nnnn_
Cuts at: 0 838 1278
Size: 838 440
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 991 1025 1278
Size: 991 34 253
Fragments arranged by size:
991 253 34
BclI T'GATC_A
Cuts at: 0 1076 1278
Size: 1076 202
BlpI GC'TnA_GC
Cuts at: 0 1043 1278
Size: 1043 235
Bme1580I G_kGCm'C
Cuts at: 0 46 225 1278
Size: 46 179 1053
Fragments arranged by size:
1053 179 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 810 1227 1278
Size: 810 417 51
Fragments arranged by size:
810 417 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 257 884 1098 1278
Size: 163 94 627 214 180
Fragments arranged by size:
627 214 180 163 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 349 747 1278
Size: 214 135 398 531
Fragments arranged by size:
531 398 214 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 430 1278
Size: 430 848
BsaHI Gr'CG_yC
Cuts at: 0 32 1278
Size: 32 1246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1278
Size: 730 30 518
Fragments arranged by size:
730 518 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 331 397 679 706 709 1237 1278
Size: 217 114 66 282 27 3 528 41
Fragments arranged by size:
528 282 217 114 66 41 27 3
BseYI C'CCAG_C
Cuts at: 0 27 39 157 1134 1278
Size: 27 12 118 977 144
Fragments arranged by size:
977 144 118 27 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 311 634 1174 1255 1278
Size: 158 153 323 540 81 23
Fragments arranged by size:
540 323 158 153 81 23
BsiEI CG_ry'CG
Cuts at: 0 741 1278
Size: 741 537
BsiHKAI G_wGCw'C
Cuts at: 0 680 701 773 965 1278
Size: 680 21 72 192 313
Fragments arranged by size:
680 313 192 72 21
Bsp1286I G_dGCh'C
Cuts at: 0 46 225 323 680 701 773 965 1204
Size: 46 179 98 357 21 72 192 239
Cuts at: 1204 1278
Size: 74
Fragments arranged by size:
357 239 192 179 98 74 72 46
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 339 363 742 1109 1278
Size: 88 251 24 379 367 169
Fragments arranged by size:
379 367 251 169 88 24
BsrFI r'CCGG_y
Cuts at: 0 607 1244 1278
Size: 607 637 34
Fragments arranged by size:
637 607 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1269 1278
Size: 1269 9
BstEII G'GTnAC_C
Cuts at: 0 75 990 1278
Size: 75 915 288
Fragments arranged by size:
915 288 75
BtgI C'CryG_G
Cuts at: 0 121 200 1278
Size: 121 79 1078
Fragments arranged by size:
1078 121 79
BtsI GCAGTG_nn'
Cuts at: 0 323 1173 1278
Size: 323 850 105
Fragments arranged by size:
850 323 105
DraIII CAC_nnn'GTG
Cuts at: 0 759 1278
Size: 759 519
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 1278
Size: 580 698
EaeI y'GGCC_r
Cuts at: 0 596 761 1170 1278
Size: 596 165 409 108
Fragments arranged by size:
596 409 165 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 703 763 1278
Size: 703 60 515
Fragments arranged by size:
703 515 60
EcoICRI GAG'CTC
Cuts at: 0 699 771 963 1278
Size: 699 72 192 315
Fragments arranged by size:
699 315 192 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 257 821 850 884 1039 1098 1278
Size: 163 94 564 29 34 155 59 180
Fragments arranged by size:
564 180 163 155 94 59 34 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 688 970 1105 1278
Size: 145 543 282 135 173
Fragments arranged by size:
543 282 173 145 135
EcoO109I rG'GnC_Cy
Cuts at: 0 132 474 669 731 870 1278
Size: 132 342 195 62 139 408
Fragments arranged by size:
408 342 195 139 132 62
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 1011 1278
Size: 177 834 267
Fragments arranged by size:
834 267 177
HaeII r_GCGC'y
Cuts at: 0 35 1278
Size: 35 1243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 730 762 778 810 1278
Size: 730 32 16 32 468
Fragments arranged by size:
730 468 32 32 16
KasI G'GCGC_C
Cuts at: 0 31 1278
Size: 31 1247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 1278
Size: 445 833
MscI TGG'CCA
Cuts at: 0 598 1172 1278
Size: 598 574 106
Fragments arranged by size:
598 574 106
MspA1I CmG'CkG
Cuts at: 0 123 262 648 1269 1278
Size: 123 139 386 621 9
Fragments arranged by size:
621 386 139 123 9
NaeI GCC'GGC
Cuts at: 0 1246 1278
Size: 1246 32
NarI GG'CG_CC
Cuts at: 0 32 1278
Size: 32 1246
NgoMIV G'CCGG_C
Cuts at: 0 1244 1278
Size: 1244 34
NspI r_CATG'y
Cuts at: 0 99 1278
Size: 99 1179
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 928 960 1278
Size: 373 32 523 32 318
Fragments arranged by size:
523 373 318 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 731 870 1278
Size: 132 599 139 408
Fragments arranged by size:
599 408 139 132
PstI C_TGCA'G
Cuts at: 0 858 1109 1180 1278
Size: 858 251 71 98
Fragments arranged by size:
858 251 98 71
PvuII CAG'CTG
Cuts at: 0 262 648 1269 1278
Size: 262 386 621 9
Fragments arranged by size:
621 386 262 9
SacI G_AGCT'C
Cuts at: 0 701 773 965 1278
Size: 701 72 192 313
Fragments arranged by size:
701 313 192 72
SacII CC_GC'GG
Cuts at: 0 124 1278
Size: 124 1154
SfcI C'TryA_G
Cuts at: 0 854 1105 1176 1278
Size: 854 251 71 102
Fragments arranged by size:
854 251 102 71
SfoI GGC'GCC
Cuts at: 0 33 1278
Size: 33 1245
SmlI C'TyrA_G
Cuts at: 0 229 364 726 1278
Size: 229 135 362 552
Fragments arranged by size:
552 362 229 135
StuI AGG'CCT
Cuts at: 0 1111 1278
Size: 1111 167
StyI C'CwwG_G
Cuts at: 0 585 956 1278
Size: 585 371 322
Fragments arranged by size:
585 371 322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 909 963 1122 1278
Size: 909 54 159 156
Fragments arranged by size:
909 159 156 54
XmnI GAAnn'nnTTC
Cuts at: 0 209 1278
Size: 209 1069
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI AvaI BanI BanII
BbeI BbsI BcgI BclI BlpI Bme1580I BmrI BpmI
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstEII BtgI BtsI
DraIII DrdI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MscI MspA1I NaeI
NarI NgoMIV NspI PpiI PpuMI PstI PvuII SacI
SacII SfcI SfoI SmlI StuI StyI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BciVI BfrBI BglI BglII
BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI EagI EarI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI