(Circular) (Six-base) MAPSORT of: pFN21ASDA1369.seq Check: 1181 from: 1 to: 6619 pFN21ASDA1369 6619 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2565 2984 3962 1937 Size: 628 419 978 4594 Fragments arranged by size: 4594 978 628 419 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 3371 4682 6066 6334 1632 Size: 1037 702 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1037 702 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2728 5518 6495 1203 Size: 1525 2790 977 1327 Fragments arranged by size: 2790 1525 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AlwNI CAG_nnn'CTG Cuts at: 3049 3754 4362 5934 3049 Size: 705 608 1572 3734 Fragments arranged by size: 3734 1572 705 608 ApaI G_GGCC'C Cuts at: 2123 2123 Size: 6619 ApaLI G'TGCA_C Cuts at: 3338 4676 5832 3338 Size: 1338 1156 4125 Fragments arranged by size: 4125 1338 1156 ApoI r'AATT_y Cuts at: 1493 2519 3285 3775 3928 4154 4306 6415 1493 Size: 1026 766 490 153 226 152 2109 1697 Fragments arranged by size: 2109 1697 1026 766 490 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 3944 1870 Size: 73 2001 4545 Fragments arranged by size: 4545 2001 73 BamHI G'GATC_C Cuts at: 2434 3949 4316 2434 Size: 1515 367 4737 Fragments arranged by size: 4737 1515 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3940 5395 618 Size: 325 217 627 2153 1455 1842 Fragments arranged by size: 2153 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2123 2874 2997 3189 3591 3938 1391 Size: 732 751 123 192 402 347 4072 Fragments arranged by size: 4072 751 732 402 347 192 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 2675 928 Size: 971 429 347 4872 Fragments arranged by size: 4872 971 429 347 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4810 4844 1903 Size: 34 2873 34 3678 Fragments arranged by size: 3678 2873 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2468 2546 3905 4511 5721 2468 Size: 78 1359 606 1210 3366 Fragments arranged by size: 3366 1359 1210 606 78 BclI T'GATC_A Cuts at: 1548 3382 1548 Size: 1834 4785 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3058 5230 136 Size: 107 122 71 631 605 1386 2172 1525 Fragments arranged by size: 2172 1525 1386 631 605 122 107 71 BglII A'GATC_T Cuts at: 3346 6614 3346 Size: 3268 3351 BlpI GC'TnA_GC Cuts at: 2868 4036 2868 Size: 1168 5451 Bme1580I G_kGCm'C Cuts at: 1790 2123 3342 4680 5836 1790 Size: 333 1219 1338 1156 2573 Fragments arranged by size: 2573 1338 1219 1156 333 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4350 5308 442 Size: 416 53 83 399 24 2933 958 1753 Fragments arranged by size: 2933 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2380 2380 Size: 6619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2981 3011 5279 1239 Size: 1022 720 30 2268 2579 Fragments arranged by size: 2579 2268 1022 720 30 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2269 2999 3852 4744 5609 5907 2269 Size: 730 853 892 865 298 2981 Fragments arranged by size: 2981 892 865 853 730 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3727 5282 882 Size: 617 2228 1555 2219 Fragments arranged by size: 2228 2219 1555 617 BsaAI yAC'GTr Cuts at: 493 1204 2257 3916 4410 493 Size: 711 1053 1659 494 2702 Fragments arranged by size: 2702 1659 1053 711 494 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4426 4808 5443 275 Size: 53 83 186 936 2893 382 635 1451 Fragments arranged by size: 2893 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5052 5450 5724 5871 6282 5052 Size: 398 274 147 411 5389 Fragments arranged by size: 5389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6394 Fragments arranged by size: 6394 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 3092 1186 Size: 889 1017 4713 Fragments arranged by size: 4713 1017 889 BseYI C'CCAG_C Cuts at: 2075 5822 6318 2075 Size: 3747 496 2376 Fragments arranged by size: 3747 2376 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3146 3403 1163 Size: 573 1410 257 4379 Fragments arranged by size: 4379 1410 573 257 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4069 4830 5858 1540 Size: 275 186 2068 761 1028 2301 Fragments arranged by size: 2301 2068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2247 2793 2997 3189 3342 3591 4680 4765 5836 Size: 546 204 192 153 249 1089 85 1071 Cuts at: 5836 2247 Size: 3030 Fragments arranged by size: 3030 1089 1071 546 249 204 192 153 85 BsmI GAATG_Cn' Cuts at: 4130 4223 4130 Size: 93 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2247 2793 2874 2997 3189 3342 Size: 399 333 124 546 81 123 192 153 Cuts at: 3342 3591 3938 4680 4765 5836 1391 Size: 249 347 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 347 333 249 192 153 124 123 85 81 BspEI T'CCGG_A Cuts at: 6282 6282 Size: 6619 BspHI T'CATG_A Cuts at: 4508 6238 4508 Size: 1730 4889 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2669 3974 844 Size: 1825 1305 3489 Fragments arranged by size: 3489 1825 1305 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2825 5114 5288 65 Size: 1183 1577 2289 174 1396 Fragments arranged by size: 2289 1577 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2095 5263 5450 1700 Size: 110 285 3168 187 2869 Fragments arranged by size: 3168 2869 285 187 110 BsrGI T'GTAC_A Cuts at: 96 3561 96 Size: 3465 3154 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 2748 4679 5691 2748 Size: 1931 1012 3676 Fragments arranged by size: 3676 1931 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 2007 5423 2007 Size: 3416 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstXI CCAn_nnnn'nTGG Cuts at: 3005 3779 3005 Size: 774 5845 BstYI r'GATC_y Cuts at: 1972 2434 3346 3533 3949 4316 4701 4718 6170 Size: 462 912 187 416 367 385 17 1452 Cuts at: 6170 6256 6268 6614 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 912 462 416 385 367 346 187 86 17 12 BstZ17I GTA'TAC Cuts at: 2566 2566 Size: 6619 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6067 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3711 487 Size: 683 56 2485 3395 Fragments arranged by size: 3395 2485 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4929 4949 4135 Size: 794 20 5805 Fragments arranged by size: 5805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 2718 2718 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 3267 4066 Size: 54 1425 50 275 18 267 1170 799 Cuts at: 4066 4955 6584 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1170 889 799 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4066 1537 Size: 275 2254 4090 Fragments arranged by size: 4090 2254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2554 2799 3461 4549 1353 Size: 699 502 245 662 1088 3423 Fragments arranged by size: 3423 1088 699 662 502 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2995 3187 3589 2995 Size: 192 402 6025 Fragments arranged by size: 6025 402 192 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2669 2981 3011 3371 4682 5279 Size: 393 629 408 312 30 360 1311 597 Cuts at: 5279 6066 6334 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1311 787 629 597 408 393 360 312 268 30 EcoNI CCTnn'n_nnAGG Cuts at: 2621 2621 Size: 6619 EcoO109I rG'GnC_Cy Cuts at: 2119 2653 2119 Size: 534 6085 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 5766 1678 Size: 4088 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2362 2394 2413 2445 2762 Size: 32 135 32 790 32 19 32 317 Cuts at: 2762 2794 3536 3568 5260 5292 5334 5366 1373 Size: 32 742 32 1692 32 42 32 2626 Fragments arranged by size: 2626 1692 790 742 317 135 42 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 2985 3121 3790 3963 4209 669 Size: 988 281 1047 136 669 173 246 3079 Fragments arranged by size: 3079 1047 988 669 281 246 173 136 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 3790 4209 3790 Size: 419 6200 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 3033 4218 3033 Size: 1185 5434 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2820 3507 5733 5917 1305 Size: 1515 687 2226 184 2007 Fragments arranged by size: 2226 2007 1515 687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3269 10 Size: 54 1425 343 1437 3360 Fragments arranged by size: 3360 1437 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2733 3170 4577 4936 5095 518 Size: 648 1093 474 437 1407 359 159 2042 Fragments arranged by size: 2042 1407 1093 648 474 437 359 159 MspA1I CmG'CkG Cuts at: 1606 3049 3178 3751 4035 4712 5860 6105 1606 Size: 1443 129 573 284 677 1148 245 2120 Fragments arranged by size: 2120 1443 1148 677 573 284 245 129 NaeI GCC'GGC Cuts at: 2097 2097 Size: 6619 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6067 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NgoMIV G'CCGG_C Cuts at: 2095 2095 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 4066 4066 Size: 6619 NspI r_CATG'y Cuts at: 1132 1229 2732 3977 5522 1132 Size: 97 1503 1245 1545 2229 Fragments arranged by size: 2229 1545 1503 1245 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6619 PciI A'CATG_T Cuts at: 2728 5518 2728 Size: 2790 3829 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6619 PfoI T'CCnGG_A Cuts at: 2997 2997 Size: 6619 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PmlI CAC'GTG Cuts at: 2257 2257 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3612 3644 4688 4720 6201 6233 3612 Size: 32 1044 32 1481 32 3998 Fragments arranged by size: 3998 1481 1044 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 2653 Size: 6619 PshAI GACnn'nnGTC Cuts at: 1613 3408 1613 Size: 1795 4824 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 2119 2119 Size: 6619 PstI C_TGCA'G Cuts at: 830 3759 3971 830 Size: 2929 212 3478 Fragments arranged by size: 3478 2929 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 3049 3178 3751 3049 Size: 129 573 5917 Fragments arranged by size: 5917 573 129 SacI G_AGCT'C Cuts at: 2997 3189 3591 2997 Size: 192 402 6025 Fragments arranged by size: 6025 402 192 SalI G'TCGA_C Cuts at: 1936 2983 3961 1936 Size: 1047 978 4594 Fragments arranged by size: 4594 1047 978 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SfcI C'TryA_G Cuts at: 826 1045 3431 3755 3800 3967 5102 5783 5974 Size: 219 2386 324 45 167 1135 681 191 Cuts at: 5974 826 Size: 1471 Fragments arranged by size: 2386 1471 1135 681 324 219 191 167 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 3946 1872 Size: 2074 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2248 2978 3867 4723 5624 5886 Size: 197 926 305 730 889 856 901 262 Cuts at: 5886 820 Size: 1553 Fragments arranged by size: 1553 926 901 889 856 730 305 262 197 SnaBI TAC'GTA Cuts at: 493 3916 4410 493 Size: 3423 494 2702 Fragments arranged by size: 3423 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 2698 3364 4543 5 Size: 47 2646 666 1179 2081 Fragments arranged by size: 2646 2081 1179 666 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6619 StyI C'CwwG_G Cuts at: 513 1065 2487 4058 513 Size: 552 1422 1571 3074 Fragments arranged by size: 3074 1571 1422 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3560 4646 4663 4816 5001 5446 325 Size: 1255 1980 1086 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 1086 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2940 3561 4865 Size: 274 80 33 51 1443 963 621 1304 Cuts at: 4865 6577 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1304 963 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6579 6611 6579 Size: 32 6587 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6619 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3034 3034 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3944 1870 Size: 2074 4545 XmnI GAAnn'nnTTC Cuts at: 2730 4748 2730 Size: 2018 4601 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI