Restriction map for
pFN21ASDA1281
(Linear) (Six-base) MAPSORT of: KIAA1281.seq Check: 7432 from: 1 to: 4536
KIAA1281.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4385 4473 4536
Size: 4385 88 63
Fragments arranged by size:
4385 88 63
AccI GT'mk_AC
Cuts at: 0 3680 3737 4536
Size: 3680 57 799
Fragments arranged by size:
3680 799 57
Acc65I G'GTAC_C
Cuts at: 0 1712 4536
Size: 1712 2824
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 3562 4536
Size: 637 2925 974
Fragments arranged by size:
2925 974 637
AfeI AGC'GCT
Cuts at: 0 455 4536
Size: 455 4081
AflIII A'CryG_T
Cuts at: 0 1060 4536
Size: 1060 3476
AgeI A'CCGG_T
Cuts at: 0 1014 4536
Size: 1014 3522
AhdI GACnn_n'nnGTC
Cuts at: 0 2859 3273 4536
Size: 2859 414 1263
Fragments arranged by size:
2859 1263 414
AleI CACnn'nnGTG
Cuts at: 0 2725 4536
Size: 2725 1811
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1839 3059 4505 4536
Size: 1839 1220 1446 31
Fragments arranged by size:
1839 1446 1220 31
AlwNI CAG_nnn'CTG
Cuts at: 0 3339 4536
Size: 3339 1197
ApaI G_GGCC'C
Cuts at: 0 1103 1242 4536
Size: 1103 139 3294
Fragments arranged by size:
3294 1103 139
ApoI r'AATT_y
Cuts at: 0 93 139 250 285 447 1125 1575 1974
Size: 93 46 111 35 162 678 450 399
Cuts at: 1974 2127 4536
Size: 153 2409
Fragments arranged by size:
2409 678 450 399 162 153 111 93
46 35
AseI AT'TA_AT
Cuts at: 0 2113 4536
Size: 2113 2423
AvaI C'yCGr_G
Cuts at: 0 382 1332 2703 3690 3974 4425 4536
Size: 382 950 1371 987 284 451 111
Fragments arranged by size:
1371 987 950 451 382 284 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1829 1862 4536
Size: 1829 33 2674
Fragments arranged by size:
2674 1829 33
BamHI G'GATC_C
Cuts at: 0 36 4536
Size: 36 4500
BanI G'GyrC_C
Cuts at: 0 422 598 698 1712 1869 2739 2748 3043
Size: 422 176 100 1014 157 870 9 295
Cuts at: 3043 4373 4536
Size: 1330 163
Fragments arranged by size:
1330 1014 870 422 295 176 163 157
100 9
BanII G_rGCy'C
Cuts at: 0 745 824 1103 1242 1339 2393 3260 3398
Size: 745 79 279 139 97 1054 867 138
Cuts at: 3398 3884 4536
Size: 486 652
Fragments arranged by size:
1054 867 745 652 486 279 139 138
97 79
BbeI G_GCGC'C
Cuts at: 0 426 602 4536
Size: 426 176 3934
Fragments arranged by size:
3934 426 176
BbvCI CC'TCA_GC
Cuts at: 0 1460 2642 3182 4536
Size: 1460 1182 540 1354
Fragments arranged by size:
1460 1354 1182 540
BclI T'GATC_A
Cuts at: 0 3197 4536
Size: 3197 1339
BfrBI ATG'CAT
Cuts at: 0 3250 4536
Size: 3250 1286
BglII A'GATC_T
Cuts at: 0 1781 4536
Size: 1781 2755
BlpI GC'TnA_GC
Cuts at: 0 3599 4316 4536
Size: 3599 717 220
Fragments arranged by size:
3599 717 220
Bme1580I G_kGCm'C
Cuts at: 0 701 763 810 1103 1242 2205 3048 3845
Size: 701 62 47 293 139 963 843 797
Cuts at: 3845 4536
Size: 691
Fragments arranged by size:
963 843 797 701 691 293 139 62
47
BmrI ACTGGGnnnn_n'
Cuts at: 0 773 1245 2294 3374 4536
Size: 773 472 1049 1080 1162
Fragments arranged by size:
1162 1080 1049 773 472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3836 4536
Size: 3836 700
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 449 1297 1518 1631 1774 4121 4240
Size: 192 257 848 221 113 143 2347 119
Cuts at: 4240 4536
Size: 296
Fragments arranged by size:
2347 848 296 257 221 192 143 119
113
Bpu10I CC'TnA_GC
Cuts at: 0 234 1460 2642 3182 4536
Size: 234 1226 1182 540 1354
Fragments arranged by size:
1354 1226 1182 540 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1586 4536
Size: 1586 2950
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 4536
Size: 292 4244
BsaAI yAC'GTr
Cuts at: 0 1184 1203 4536
Size: 1184 19 3333
Fragments arranged by size:
3333 1184 19
BsaHI Gr'CG_yC
Cuts at: 0 423 599 4536
Size: 423 176 3937
Fragments arranged by size:
3937 423 176
BsaWI w'CCGG_w
Cuts at: 0 1014 3477 4536
Size: 1014 2463 1059
Fragments arranged by size:
2463 1059 1014
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1516 1546 4536
Size: 1516 30 2990
Fragments arranged by size:
2990 1516 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 871 874 877 880 3386 3547 3892
Size: 580 291 3 3 3 2506 161 345
Cuts at: 3892 3934 3987 4536
Size: 42 53 549
Fragments arranged by size:
2506 580 549 345 291 161 53 42
3 3 3
BseYI C'CCAG_C
Cuts at: 0 456 1966 2223 2406 2505 2680 3411 3844
Size: 456 1510 257 183 99 175 731 433
Cuts at: 3844 4069 4536
Size: 225 467
Fragments arranged by size:
1510 731 467 456 433 257 225 183
175 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1206 4363 4536
Size: 1206 3157 173
Fragments arranged by size:
3157 1206 173
BsiHKAI G_wGCw'C
Cuts at: 0 3073 3398 4536
Size: 3073 325 1138
Fragments arranged by size:
3073 1138 325
BsiWI C'GTAC_G
Cuts at: 0 1199 4536
Size: 1199 3337
BsmI GAATG_Cn'
Cuts at: 0 1828 2719 2934 4536
Size: 1828 891 215 1602
Fragments arranged by size:
1828 1602 891 215
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2470 4536
Size: 2470 2066
Bsp1286I G_dGCh'C
Cuts at: 0 701 745 763 810 824 1103 1242 1339
Size: 701 44 18 47 14 279 139 97
Cuts at: 1339 2205 2393 3048 3073 3260 3398 3845 3884
Size: 866 188 655 25 187 138 447 39
Cuts at: 3884 4536
Size: 652
Fragments arranged by size:
866 701 655 652 447 279 188 187
139 138 97 47 44 39 25 18
14
BspEI T'CCGG_A
Cuts at: 0 3477 4536
Size: 3477 1059
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 656 2849 3027 4385 4473 4536
Size: 656 2193 178 1358 88 63
Fragments arranged by size:
2193 1358 656 178 88 63
BsrBI CCG'CTC
Cuts at: 0 4320 4536
Size: 4320 216
BsrDI GCAATG_nn'
Cuts at: 0 732 1941 2210 4536
Size: 732 1209 269 2326
Fragments arranged by size:
2326 1209 732 269
BsrFI r'CCGG_y
Cuts at: 0 1014 1872 4536
Size: 1014 858 2664
Fragments arranged by size:
2664 1014 858
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 1318 4536
Size: 648 670 3218
Fragments arranged by size:
3218 670 648
BstEII G'GTnAC_C
Cuts at: 0 4411 4536
Size: 4411 125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 667 2382 3753 4536
Size: 667 1715 1371 783
Fragments arranged by size:
1715 1371 783 667
BstYI r'GATC_y
Cuts at: 0 36 1781 4536
Size: 36 1745 2755
Fragments arranged by size:
2755 1745 36
BstZ17I GTA'TAC
Cuts at: 0 3738 4536
Size: 3738 798
Bsu36I CC'TnA_GG
Cuts at: 0 1708 4536
Size: 1708 2828
BtgI C'CryG_G
Cuts at: 0 533 4536
Size: 533 4003
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 83 4536
Size: 83 4453
BtsI GCAGTG_nn'
Cuts at: 0 678 750 1833 2258 4536
Size: 678 72 1083 425 2278
Fragments arranged by size:
2278 1083 678 425 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 174 209 714 749 4536
Size: 174 35 505 35 3787
Fragments arranged by size:
3787 505 174 35 35
DraI TTT'AAA
Cuts at: 0 2561 3699 4536
Size: 2561 1138 837
Fragments arranged by size:
2561 1138 837
EaeI y'GGCC_r
Cuts at: 0 2627 3240 3284 3449 4365 4536
Size: 2627 613 44 165 916 171
Fragments arranged by size:
2627 916 613 171 165 44
EarI CTCTTCn'nnn_
Cuts at: 0 848 1300 1647 4175 4524 4536
Size: 848 452 347 2528 349 12
Fragments arranged by size:
2528 848 452 349 347 12
EcoICRI GAG'CTC
Cuts at: 0 3396 4536
Size: 3396 1140
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 192 449 637 1297 1518 1631 1774 3562
Size: 192 257 188 660 221 113 143 1788
Cuts at: 3562 4121 4240 4536
Size: 559 119 296
Fragments arranged by size:
1788 660 559 296 257 221 192 188
143 119 113
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 2690 4445 4536
Size: 652 2038 1755 91
Fragments arranged by size:
2038 1755 652 91
EcoO109I rG'GnC_Cy
Cuts at: 0 684 1100 1239 1904 1992 4536
Size: 684 416 139 665 88 2544
Fragments arranged by size:
2544 684 665 416 139 88
EcoRI G'AATT_C
Cuts at: 0 285 447 1974 4536
Size: 285 162 1527 2562
Fragments arranged by size:
2562 1527 285 162
EcoRV GAT'ATC
Cuts at: 0 1467 4536
Size: 1467 3069
HaeII r_GCGC'y
Cuts at: 0 426 457 602 934 2702 4536
Size: 426 31 145 332 1768 1834
Fragments arranged by size:
1834 1768 426 332 145 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1516 1548 1768 1800 2041 2073 2378 2410
Size: 1516 32 220 32 241 32 305 32
Cuts at: 2410 2845 2877 3259 3291 3673 3705 3895 3927
Size: 435 32 382 32 382 32 190 32
Cuts at: 3927 3977 3999 4009 4031 4339 4371 4536
Size: 50 22 10 22 308 32 165
Fragments arranged by size:
1516 435 382 382 308 305 241 220
190 165 50 32 32 32 32 32
32 32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 0 909 2765 3863 4215 4536
Size: 909 1856 1098 352 321
Fragments arranged by size:
1856 1098 909 352 321
HpaI GTT'AAC
Cuts at: 0 3863 4536
Size: 3863 673
KasI G'GCGC_C
Cuts at: 0 422 598 4536
Size: 422 176 3938
Fragments arranged by size:
3938 422 176
KpnI G_GTAC'C
Cuts at: 0 1716 4536
Size: 1716 2820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 1050 2054 2585 2843 4536
Size: 69 981 1004 531 258 1693
Fragments arranged by size:
1693 1004 981 531 258 69
MscI TGG'CCA
Cuts at: 0 2629 3242 3286 4536
Size: 2629 613 44 1250
Fragments arranged by size:
2629 1250 613 44
MslI CAynn'nnrTG
Cuts at: 0 665 704 1821 2380 2398 2725 2996 3731
Size: 665 39 1117 559 18 327 271 735
Cuts at: 3731 4536
Size: 805
Fragments arranged by size:
1117 805 735 665 559 327 271 39
18
MspA1I CmG'CkG
Cuts at: 0 476 485 535 784 1430 2791 3055 4315
Size: 476 9 50 249 646 1361 264 1260
Cuts at: 4315 4371 4536
Size: 56 165
Fragments arranged by size:
1361 1260 646 476 264 249 165 56
50 9
NarI GG'CG_CC
Cuts at: 0 423 599 4536
Size: 423 176 3937
Fragments arranged by size:
3937 423 176
NdeI CA'TA_TG
Cuts at: 0 3252 4536
Size: 3252 1284
NsiI A_TGCA'T
Cuts at: 0 3252 4536
Size: 3252 1284
NspI r_CATG'y
Cuts at: 0 1064 2761 3027 4042 4267 4536
Size: 1064 1697 266 1015 225 269
Fragments arranged by size:
1697 1064 1015 269 266 225
PasI CC'CwG_GG
Cuts at: 0 279 3031 4536
Size: 279 2752 1505
Fragments arranged by size:
2752 1505 279
PciI A'CATG_T
Cuts at: 0 1060 4536
Size: 1060 3476
PflMI CCAn_nnn'nTGG
Cuts at: 0 3339 4536
Size: 3339 1197
PfoI T'CCnGG_A
Cuts at: 0 628 4137 4518 4536
Size: 628 3509 381 18
Fragments arranged by size:
3509 628 381 18
PmlI CAC'GTG
Cuts at: 0 1184 4536
Size: 1184 3352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1522 1554 4536
Size: 1522 32 2982
Fragments arranged by size:
2982 1522 32
PpuMI rG'GwC_Cy
Cuts at: 0 1904 4536
Size: 1904 2632
PspOMI G'GGCC_C
Cuts at: 0 1099 1238 4536
Size: 1099 139 3298
Fragments arranged by size:
3298 1099 139
PspXI vC'TCGA_Gb
Cuts at: 0 2703 4536
Size: 2703 1833
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1183 1215 4536
Size: 1183 32 3321
Fragments arranged by size:
3321 1183 32
PstI C_TGCA'G
Cuts at: 0 24 789 1373 3054 4536
Size: 24 765 584 1681 1482
Fragments arranged by size:
1681 1482 765 584 24
PvuII CAG'CTG
Cuts at: 0 2791 3055 4315 4536
Size: 2791 264 1260 221
Fragments arranged by size:
2791 1260 264 221
SacI G_AGCT'C
Cuts at: 0 3398 4536
Size: 3398 1138
SacII CC_GC'GG
Cuts at: 0 536 4536
Size: 536 4000
SanDI GG'GwC_CC
Cuts at: 0 1904 4536
Size: 1904 2632
SapI GCTCTTCn'nnn_
Cuts at: 0 1647 4536
Size: 1647 2889
ScaI AGT'ACT
Cuts at: 0 3322 4033 4536
Size: 3322 711 503
Fragments arranged by size:
3322 711 503
SfcI C'TryA_G
Cuts at: 0 20 785 1369 2945 3050 3300 3635 4310
Size: 20 765 584 1576 105 250 335 675
Cuts at: 4310 4536
Size: 226
Fragments arranged by size:
1576 765 675 584 335 250 226 105
20
SfoI GGC'GCC
Cuts at: 0 424 600 4536
Size: 424 176 3936
Fragments arranged by size:
3936 424 176
SmaI CCC'GGG
Cuts at: 0 1334 4427 4536
Size: 1334 3093 109
Fragments arranged by size:
3093 1334 109
SmlI C'TyrA_G
Cuts at: 0 1601 2703 3690 4536
Size: 1601 1102 987 846
Fragments arranged by size:
1601 1102 987 846
SpeI A'CTAG_T
Cuts at: 0 169 4536
Size: 169 4367
SphI G_CATG'C
Cuts at: 0 4042 4267 4536
Size: 4042 225 269
Fragments arranged by size:
4042 269 225
SspI AAT'ATT
Cuts at: 0 1005 4536
Size: 1005 3531
StuI AGG'CCT
Cuts at: 0 268 4536
Size: 268 4268
StyI C'CwwG_G
Cuts at: 0 185 417 1632 2525 4536
Size: 185 232 1215 893 2011
Fragments arranged by size:
2011 1215 893 232 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1039 1685 4017 4411 4536
Size: 1039 646 2332 394 125
Fragments arranged by size:
2332 1039 646 394 125
TatI w'GTAC_w
Cuts at: 0 1688 3320 4031 4536
Size: 1688 1632 711 505
Fragments arranged by size:
1688 1632 711 505
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 3971 4003 4536
Size: 17 32 3922 32 533
Fragments arranged by size:
3922 533 32 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 424 670 1125 3250 4399 4536
Size: 424 246 455 2125 1149 137
Fragments arranged by size:
2125 1149 455 424 246 137
XhoI C'TCGA_G
Cuts at: 0 2703 3690 4536
Size: 2703 987 846
Fragments arranged by size:
2703 987 846
XmaI C'CCGG_G
Cuts at: 0 1332 4425 4536
Size: 1332 3093 111
Fragments arranged by size:
3093 1332 111
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HpaI
KasI KpnI MmeI MscI MslI MspA1I NarI NdeI
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PsrI PstI PvuII SacI SacII
SanDI SapI ScaI SfcI SfoI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AloI ApaLI AscI AsiSI AvrII
BbsI BcgI BciVI BglI BmgBI BmtI BsaBI BsiEI
BspHI BsrGI BssHII BssSI BstBI ClaI DraIII DrdI
EagI EciI FalI FseI FspI FspAI HindIII MfeI
MluI NaeI NcoI NgoMIV NheI NotI NruI PacI
PmeI PshAI PsiI PvuI RsrII SalI SbfI SexAI
SfiI SgfI SgrAI SnaBI SrfI SwaI Tth111I XbaI
XmnI ZraI