(Circular) (Six-base) MAPSORT of: pFN21ASDA1279.seq Check: 7530 from: 1 to: 6592 pFN21ASDA1279 6592 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4402 278 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 3399 3935 1937 Size: 736 726 536 4594 Fragments arranged by size: 4594 736 726 536 Acc65I G'GTAC_C Cuts at: 2403 3913 2403 Size: 1510 5082 AclI AA'CG_TT Cuts at: 1563 2031 4719 5092 1563 Size: 468 2688 373 3063 Fragments arranged by size: 3063 2688 468 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2610 2922 2928 3584 4655 6039 6307 1632 Size: 978 312 6 656 1071 1384 268 1917 Fragments arranged by size: 1917 1384 1071 978 656 312 268 6 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6395 AflIII A'CryG_T Cuts at: 1203 5491 6468 1203 Size: 4288 977 1327 Fragments arranged by size: 4288 1327 977 AgeI A'CCGG_T Cuts at: 3122 5423 3122 Size: 2301 4291 AhdI GACnn_n'nnGTC Cuts at: 5321 5321 Size: 6592 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6592 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2065 1184 Size: 881 5711 AlwNI CAG_nnn'CTG Cuts at: 2683 2835 4335 5907 2683 Size: 152 1500 1572 3368 Fragments arranged by size: 3368 1572 1500 152 ApaI G_GGCC'C Cuts at: 2280 2280 Size: 6592 ApaLI G'TGCA_C Cuts at: 4649 5805 4649 Size: 1156 5436 ApoI r'AATT_y Cuts at: 1493 2057 3724 3752 3901 4127 4279 6388 1493 Size: 564 1667 28 149 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 564 226 152 149 28 AseI AT'TA_AT Cuts at: 160 1032 5146 160 Size: 872 4114 1606 Fragments arranged by size: 4114 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2221 2270 2324 3917 1870 Size: 73 196 82 49 54 1593 4545 Fragments arranged by size: 4545 1593 196 82 73 54 49 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2443 2476 2443 Size: 33 6559 BamHI G'GATC_C Cuts at: 3511 3922 4289 3511 Size: 411 367 5814 Fragments arranged by size: 5814 411 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2225 2403 3913 5368 618 Size: 325 217 627 438 178 1510 1455 1842 Fragments arranged by size: 1842 1510 1455 627 438 325 217 178 BanII G_rGCy'C Cuts at: 1391 2271 2280 3390 3911 1391 Size: 880 9 1110 521 4072 Fragments arranged by size: 4072 1110 880 521 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2898 3830 928 Size: 971 999 932 3690 Fragments arranged by size: 3690 999 971 932 BbvCI CC'TCA_GC Cuts at: 2256 2256 Size: 6592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4783 4817 1903 Size: 34 2846 34 3678 Fragments arranged by size: 3678 2846 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4484 5694 4484 Size: 1210 5382 BclI T'GATC_A Cuts at: 1548 1548 Size: 6592 BfrBI ATG'CAT Cuts at: 3361 3361 Size: 6592 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2275 5203 136 Size: 107 122 71 631 605 603 2928 1525 Fragments arranged by size: 2928 1525 631 605 603 122 107 71 BglII A'GATC_T Cuts at: 2877 6587 2877 Size: 3710 2882 BlpI GC'TnA_GC Cuts at: 4009 4009 Size: 6592 Bme1580I G_kGCm'C Cuts at: 1790 2280 4653 5809 1790 Size: 490 2373 1156 2573 Fragments arranged by size: 2573 2373 1156 490 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6592 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4323 5281 442 Size: 416 53 83 399 24 2906 958 1753 Fragments arranged by size: 2906 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6592 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2577 2577 Size: 6592 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5252 1239 Size: 4013 2579 Bpu10I CC'TnA_GC Cuts at: 2256 3506 3618 2256 Size: 1250 112 5230 Fragments arranged by size: 5230 1250 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2652 2759 3193 4717 5582 5880 2652 Size: 107 434 1524 865 298 3364 Fragments arranged by size: 3364 1524 865 434 298 107 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3169 3591 5255 882 Size: 617 1670 422 1664 2219 Fragments arranged by size: 2219 1670 1664 617 422 BsaAI yAC'GTr Cuts at: 493 1204 3889 4383 493 Size: 711 2685 494 2702 Fragments arranged by size: 2702 2685 711 494 BsaBI GATnn'nnATC Cuts at: 6586 6586 Size: 6592 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4399 4781 5416 275 Size: 53 83 186 936 2866 382 635 1451 Fragments arranged by size: 2866 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2104 3122 5025 5423 5697 5844 6255 2104 Size: 1018 1903 398 274 147 411 2441 Fragments arranged by size: 2441 1903 1018 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 3335 3365 1178 Size: 30 165 30 936 30 966 30 4405 Fragments arranged by size: 4405 966 936 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 2381 2550 3098 1186 Size: 1177 18 169 548 4680 Fragments arranged by size: 4680 1177 548 169 18 BseYI C'CCAG_C Cuts at: 2286 5795 6291 2286 Size: 3509 496 2587 Fragments arranged by size: 3509 2587 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2478 3131 1163 Size: 573 742 653 4624 Fragments arranged by size: 4624 742 653 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4042 4803 5831 1540 Size: 275 186 2041 761 1028 2301 Fragments arranged by size: 2301 2041 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2373 2745 3307 4653 4738 5809 2373 Size: 372 562 1346 85 1071 3156 Fragments arranged by size: 3156 1346 1071 562 372 85 BsmI GAATG_Cn' Cuts at: 2998 3093 3323 4103 4196 2998 Size: 95 230 780 93 5394 Fragments arranged by size: 5394 780 230 95 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3340 3683 3340 Size: 343 6249 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2271 2280 2373 2745 3307 3390 3911 Size: 399 481 9 93 372 562 83 521 Cuts at: 3911 4653 4738 5809 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 521 481 399 372 93 85 83 9 BspEI T'CCGG_A Cuts at: 2104 6255 2104 Size: 4151 2441 BspHI T'CATG_A Cuts at: 4481 6211 4481 Size: 1730 4862 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2391 3947 844 Size: 1547 1556 3489 Fragments arranged by size: 3489 1556 1547 BsrBI CCG'CTC Cuts at: 1394 1823 2200 2294 4479 1394 Size: 429 377 94 2185 3507 Fragments arranged by size: 3507 2185 429 377 94 BsrDI GCAATG_nn' Cuts at: 65 1248 3492 5087 5261 65 Size: 1183 2244 1595 174 1396 Fragments arranged by size: 2244 1595 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2228 2251 2406 3122 5236 5423 1700 Size: 110 418 23 155 716 2114 187 2869 Fragments arranged by size: 2869 2114 716 418 187 155 110 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6592 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6592 BssSI C'ACGA_G Cuts at: 4652 5664 4652 Size: 1012 5580 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6592 BstBI TT'CG_AA Cuts at: 2007 5396 2007 Size: 3389 3203 BstEII G'GTnAC_C Cuts at: 1184 2231 1184 Size: 1047 5545 BstYI r'GATC_y Cuts at: 1972 2877 3438 3511 3703 3922 4289 4674 4691 Size: 905 561 73 192 219 367 385 17 Cuts at: 4691 6143 6229 6241 6587 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 905 561 385 367 346 219 192 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 2184 2205 3783 2184 Size: 21 1578 4993 Fragments arranged by size: 4993 1578 21 BtgI C'CryG_G Cuts at: 513 1065 2022 2036 513 Size: 552 957 14 5069 Fragments arranged by size: 5069 957 552 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5853 Fragments arranged by size: 5853 683 56 BtsI GCAGTG_nn' Cuts at: 4108 4902 4922 4108 Size: 794 20 5778 Fragments arranged by size: 5778 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6592 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6557 DraI TTT'AAA Cuts at: 3896 3896 Size: 6592 DraIII CAC_nnn'GTG Cuts at: 2377 2377 Size: 6592 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5599 809 Size: 507 4283 1802 Fragments arranged by size: 4283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4039 4928 6557 Size: 54 1425 50 275 18 2209 889 1629 Cuts at: 6557 8 Size: 43 Fragments arranged by size: 2209 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4039 1537 Size: 275 2227 4090 Fragments arranged by size: 4090 2227 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2589 3150 3584 4522 1353 Size: 795 441 561 434 938 3423 Fragments arranged by size: 3423 938 795 561 441 434 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5179 5553 5699 387 Size: 1064 138 3590 374 146 1280 Fragments arranged by size: 3590 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2610 2922 2928 3584 4655 5252 6039 Size: 393 978 312 6 656 1071 597 787 Cuts at: 6039 6307 1239 Size: 268 1524 Fragments arranged by size: 1524 1071 978 787 656 597 393 312 268 6 EcoO109I rG'GnC_Cy Cuts at: 2276 2276 Size: 6592 EcoRI G'AATT_C Cuts at: 3901 4279 3901 Size: 378 6214 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2618 2833 3656 2618 Size: 215 823 5554 Fragments arranged by size: 5554 823 215 FspI TGC'GCA Cuts at: 2399 5098 2399 Size: 2699 3893 HaeII r_GCGC'y Cuts at: 1678 2072 2139 2147 2168 5739 1678 Size: 394 67 8 21 3571 2531 Fragments arranged by size: 3571 2531 394 67 21 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2411 2443 3029 Size: 32 135 32 767 32 40 32 586 Cuts at: 3029 3061 3128 3160 3820 3852 5233 5265 5307 Size: 32 67 32 660 32 1381 32 42 Cuts at: 5307 5339 1373 Size: 32 2626 Fragments arranged by size: 2626 1381 767 660 586 135 67 42 40 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3430 3936 4182 669 Size: 988 281 1492 506 246 3079 Fragments arranged by size: 3079 1492 988 506 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6592 HpaI GTT'AAC Cuts at: 4182 4182 Size: 6592 KpnI G_GTAC'C Cuts at: 2407 3917 2407 Size: 1510 5082 MfeI C'AATT_G Cuts at: 4191 4191 Size: 6592 MluI A'CGCG_T Cuts at: 6468 6468 Size: 6592 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5706 5890 1305 Size: 4401 184 2007 Fragments arranged by size: 4401 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4770 Fragments arranged by size: 4770 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4550 4909 5068 518 Size: 648 3384 359 159 2042 Fragments arranged by size: 3384 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2846 4008 4685 5833 6078 1606 Size: 1240 1162 677 1148 245 2120 Fragments arranged by size: 2120 1240 1162 1148 677 245 NaeI GCC'GGC Cuts at: 2253 2253 Size: 6592 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5083 Fragments arranged by size: 5083 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6592 NgoMIV G'CCGG_C Cuts at: 2251 2251 Size: 6592 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6592 NotI GC'GGCC_GC Cuts at: 4039 4039 Size: 6592 NsiI A_TGCA'T Cuts at: 3363 3363 Size: 6592 NspI r_CATG'y Cuts at: 1132 1229 3461 3950 5495 1132 Size: 97 2232 489 1545 2229 Fragments arranged by size: 2232 2229 1545 489 97 PasI CC'CwG_GG Cuts at: 1348 2242 1348 Size: 894 5698 PciI A'CATG_T Cuts at: 5491 5491 Size: 6592 PflMI CCAn_nnn'nTGG Cuts at: 1108 2242 2683 1108 Size: 1134 441 5017 Fragments arranged by size: 5017 1134 441 PmeI GTTT'AAAC Cuts at: 3896 3896 Size: 6592 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4661 4693 6174 6206 4661 Size: 32 1481 32 5047 Fragments arranged by size: 5047 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6592 PsiI TTA'TAA Cuts at: 4162 4162 Size: 6592 PspOMI G'GGCC_C Cuts at: 2276 2276 Size: 6592 PstI C_TGCA'G Cuts at: 830 3151 3703 3944 830 Size: 2321 552 241 3478 Fragments arranged by size: 3478 2321 552 241 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6592 PvuII CAG'CTG Cuts at: 2846 2846 Size: 6592 SalI G'TCGA_C Cuts at: 1936 3934 1936 Size: 1998 4594 SapI GCTCTTCn'nnn_ Cuts at: 3584 3584 Size: 6592 SbfI CC_TGCA'GG Cuts at: 3944 3944 Size: 6592 ScaI AGT'ACT Cuts at: 3569 4840 6552 3569 Size: 1271 1712 3609 Fragments arranged by size: 3609 1712 1271 SexAI A'CCwGG_T Cuts at: 2373 2373 Size: 6592 SfcI C'TryA_G Cuts at: 826 1045 2760 3147 3396 3699 3940 5075 5756 Size: 219 1715 387 249 303 241 1135 681 Cuts at: 5756 5947 826 Size: 191 1471 Fragments arranged by size: 1715 1471 1135 681 387 303 249 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6592 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6592 SmaI CCC'GGG Cuts at: 1872 2141 2223 3919 1872 Size: 269 82 1696 4545 Fragments arranged by size: 4545 1696 269 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 2667 2738 3172 4696 5597 5859 Size: 197 926 724 71 434 1524 901 262 Cuts at: 5859 820 Size: 1553 Fragments arranged by size: 1553 1524 926 901 724 434 262 197 71 SnaBI TAC'GTA Cuts at: 493 3889 4383 493 Size: 3396 494 2702 Fragments arranged by size: 3396 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6592 SphI G_CATG'C Cuts at: 1132 3950 1132 Size: 2818 3774 SrfI GCCC'GGGC Cuts at: 2141 2141 Size: 6592 SspI AAT'ATT Cuts at: 5 52 4516 5 Size: 47 4464 2081 Fragments arranged by size: 4464 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6592 StyI C'CwwG_G Cuts at: 513 1065 2022 4031 513 Size: 552 957 2009 3074 Fragments arranged by size: 3074 2009 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4619 4636 4789 4974 5419 325 Size: 1255 3039 17 153 185 445 1498 Fragments arranged by size: 3039 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2720 3567 4838 Size: 274 80 33 51 1443 743 847 1271 Cuts at: 4838 6550 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1271 847 743 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3335 3367 6552 6584 3335 Size: 32 3185 32 3343 Fragments arranged by size: 3343 3185 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6592 XbaI T'CTAG_A Cuts at: 3928 3928 Size: 6592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3715 3715 Size: 6592 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6592 XmaI C'CCGG_G Cuts at: 1870 2139 2221 3917 1870 Size: 269 82 1696 4545 Fragments arranged by size: 4545 1696 269 82 XmnI GAAnn'nnTTC Cuts at: 3093 3636 4721 3093 Size: 543 1085 4964 Fragments arranged by size: 4964 1085 543 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4400 276 Size: 53 83 186 936 2866 2468 Fragments arranged by size: 2866 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SwaI