(Circular) (Six-base) MAPSORT of: pFN21ASDA1260.seq Check: 2607 from: 1 to: 7177 pFN21ASDA1260 7177 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4295 4295 Size: 7177 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3066 3573 4987 278 Size: 53 83 186 936 1530 507 1414 2468 Fragments arranged by size: 2468 1530 1414 936 507 186 83 53 AccI GT'mk_AC Cuts at: 1937 4470 4520 1937 Size: 2533 50 4594 Fragments arranged by size: 4594 2533 50 Acc65I G'GTAC_C Cuts at: 4498 4498 Size: 7177 AclI AA'CG_TT Cuts at: 1563 5304 5677 1563 Size: 3741 373 3063 Fragments arranged by size: 3741 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 3273 3529 4399 5240 6624 6892 1632 Size: 1541 100 256 870 841 1384 268 1917 Fragments arranged by size: 1917 1541 1384 870 841 268 256 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6980 AflIII A'CryG_T Cuts at: 1203 6076 7053 1203 Size: 4873 977 1327 Fragments arranged by size: 4873 1327 977 AgeI A'CCGG_T Cuts at: 2660 6008 2660 Size: 3348 3829 AhdI GACnn_n'nnGTC Cuts at: 5906 5906 Size: 7177 AleI CACnn'nnGTG Cuts at: 1166 2716 4248 1166 Size: 1550 1532 4095 Fragments arranged by size: 4095 1550 1532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7177 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3641 3673 3641 Size: 32 7145 AlwNI CAG_nnn'CTG Cuts at: 2041 2362 3088 3995 4920 6492 2041 Size: 321 726 907 925 1572 2726 Fragments arranged by size: 2726 1572 925 907 726 321 ApaLI G'TGCA_C Cuts at: 5234 6390 5234 Size: 1156 6021 ApoI r'AATT_y Cuts at: 1493 4438 4486 4712 4864 6973 1493 Size: 2945 48 226 152 2109 1697 Fragments arranged by size: 2945 2109 1697 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 5731 160 Size: 872 4699 1606 Fragments arranged by size: 4699 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 AvaI C'yCGr_G Cuts at: 1870 1943 2779 4502 1870 Size: 73 836 1723 4545 Fragments arranged by size: 4545 1723 836 73 BamHI G'GATC_C Cuts at: 4507 4874 4507 Size: 367 6810 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 2864 3893 4131 4149 Size: 325 217 627 774 303 1029 238 18 Cuts at: 4149 4498 5953 618 Size: 349 1455 1842 Fragments arranged by size: 1842 1455 1029 774 627 349 325 303 238 217 18 BanII G_rGCy'C Cuts at: 1391 2694 3000 3543 4496 1391 Size: 1303 306 543 953 4072 Fragments arranged by size: 4072 1303 953 543 306 BbeI G_GCGC'C Cuts at: 4135 4153 4135 Size: 18 7159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2460 3502 928 Size: 971 561 1042 4603 Fragments arranged by size: 4603 1042 971 561 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5368 5402 1903 Size: 34 3431 34 3678 Fragments arranged by size: 3678 3431 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2166 2413 3643 5069 6279 2166 Size: 247 1230 1426 1210 3064 Fragments arranged by size: 3064 1426 1230 1210 247 BclI T'GATC_A Cuts at: 1548 2503 2616 1548 Size: 955 113 6109 Fragments arranged by size: 6109 955 113 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4067 5788 136 Size: 107 122 71 631 605 2395 1721 1525 Fragments arranged by size: 2395 1721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2217 7172 2217 Size: 4955 2222 BlpI GC'TnA_GC Cuts at: 3578 4594 3578 Size: 1016 6161 Bme1580I G_kGCm'C Cuts at: 1790 2354 2484 2564 5238 6394 1790 Size: 564 130 80 2674 1156 2573 Fragments arranged by size: 2674 2573 1156 564 130 80 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7177 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3310 4149 4908 Size: 416 53 83 399 24 1893 839 759 Cuts at: 4908 5866 442 Size: 958 1753 Fragments arranged by size: 1893 1753 958 839 759 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7177 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2292 5837 1239 Size: 1053 3545 2579 Fragments arranged by size: 3545 2579 1053 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5302 6167 6465 5302 Size: 865 298 6014 Fragments arranged by size: 6014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2840 5840 882 Size: 617 1341 3000 2219 Fragments arranged by size: 3000 2219 1341 617 BsaAI yAC'GTr Cuts at: 493 1204 2479 4474 4968 493 Size: 711 1275 1995 494 2702 Fragments arranged by size: 2702 1995 1275 711 494 BsaBI GATnn'nnATC Cuts at: 3630 7171 3630 Size: 3541 3636 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3063 3132 3194 3570 Size: 53 83 186 936 1530 69 62 376 Cuts at: 3570 4070 4132 4150 4346 4984 5366 6001 275 Size: 500 62 18 196 638 382 635 1451 Fragments arranged by size: 1530 1451 936 638 635 500 382 376 196 186 83 69 62 62 53 18 BsaWI w'CCGG_w Cuts at: 2660 5610 6008 6282 6429 6840 2660 Size: 2950 398 274 147 411 2997 Fragments arranged by size: 2997 2950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6952 Fragments arranged by size: 6952 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2741 4067 1186 Size: 1555 1326 4296 Fragments arranged by size: 4296 1555 1326 BseYI C'CCAG_C Cuts at: 2355 2786 2880 3127 3467 3471 3937 6380 6876 Size: 431 94 247 340 4 466 2443 496 Cuts at: 6876 2355 Size: 2656 Fragments arranged by size: 2656 2443 496 466 431 340 247 94 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3383 1163 Size: 573 1647 4957 Fragments arranged by size: 4957 1647 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4322 4627 5388 6416 1540 Size: 275 186 2321 305 761 1028 2301 Fragments arranged by size: 2321 2301 1028 761 305 275 186 BsiHKAI G_wGCw'C Cuts at: 3000 3543 5238 5323 6394 3000 Size: 543 1695 85 1071 3783 Fragments arranged by size: 3783 1695 1071 543 85 BsmI GAATG_Cn' Cuts at: 2975 4688 4781 2975 Size: 1713 93 5371 Fragments arranged by size: 5371 1713 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3317 3317 Size: 7177 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 2484 2564 2694 3000 3543 4496 Size: 399 564 130 80 130 306 543 953 Cuts at: 4496 5238 5323 6394 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 953 742 564 543 399 306 130 130 85 80 BspEI T'CCGG_A Cuts at: 6840 6840 Size: 7177 BspHI T'CATG_A Cuts at: 2500 3591 5066 6796 2500 Size: 1091 1475 1730 2881 Fragments arranged by size: 2881 1730 1475 1091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3405 4295 4532 844 Size: 2561 890 237 3489 Fragments arranged by size: 3489 2561 890 237 BsrBI CCG'CTC Cuts at: 1394 1823 2862 5064 1394 Size: 429 1039 2202 3507 Fragments arranged by size: 3507 2202 1039 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4056 5672 5846 65 Size: 1183 2808 1616 174 1396 Fragments arranged by size: 2808 1616 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2564 2660 2899 3046 4317 5821 6008 Size: 110 754 96 239 147 1271 1504 187 Cuts at: 6008 1700 Size: 2869 Fragments arranged by size: 2869 1504 1271 754 239 187 147 110 96 BsrGI T'GTAC_A Cuts at: 96 3291 96 Size: 3195 3982 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7177 BssSI C'ACGA_G Cuts at: 4244 5237 6249 4244 Size: 993 1012 5172 Fragments arranged by size: 5172 1012 993 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 4396 794 Size: 1595 2007 3575 Fragments arranged by size: 3575 2007 1595 BstBI TT'CG_AA Cuts at: 2007 5981 2007 Size: 3974 3203 BstEII G'GTnAC_C Cuts at: 1184 2663 1184 Size: 1479 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2962 3229 2962 Size: 267 6910 BstYI r'GATC_y Cuts at: 1972 2217 2372 2546 3084 4507 4874 5259 5276 Size: 245 155 174 538 1423 367 385 17 Cuts at: 5276 6728 6814 6826 7172 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1423 538 385 367 346 245 174 155 86 17 12 BstZ17I GTA'TAC Cuts at: 4471 4471 Size: 7177 Bsu36I CC'TnA_GG Cuts at: 3645 3996 3645 Size: 351 6826 BtgI C'CryG_G Cuts at: 513 1065 2484 2539 2955 3381 3487 3585 4447 Size: 552 1419 55 416 426 106 98 862 Cuts at: 4447 513 Size: 3243 Fragments arranged by size: 3243 1419 862 552 426 416 106 98 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6438 Fragments arranged by size: 6438 683 56 BtsI GCAGTG_nn' Cuts at: 2711 2892 3445 4693 5487 5507 2711 Size: 181 553 1248 794 20 4381 Fragments arranged by size: 4381 1248 794 553 181 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7177 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3762 3797 529 Size: 35 3198 35 3909 Fragments arranged by size: 3909 3198 35 35 DraI TTT'AAA Cuts at: 4481 4481 Size: 7177 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3062 4007 6184 809 Size: 507 1746 945 2177 1802 Fragments arranged by size: 2177 1802 1746 945 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2901 3021 3384 Size: 54 1425 50 275 18 1071 120 363 Cuts at: 3384 3920 4624 5513 7142 8 Size: 536 704 889 1629 43 Fragments arranged by size: 1629 1425 1071 889 704 536 363 275 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4624 1537 Size: 275 2812 4090 Fragments arranged by size: 4090 2812 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2843 3548 4085 4197 5107 1353 Size: 1490 705 537 112 910 3423 Fragments arranged by size: 3423 1490 910 705 537 112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3346 5764 6138 6284 387 Size: 1064 138 1757 2418 374 146 1280 Fragments arranged by size: 2418 1757 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2998 3541 2998 Size: 543 6634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2292 3173 3273 3529 4399 5240 5837 Size: 393 660 881 100 256 870 841 597 Cuts at: 5837 6624 6892 1239 Size: 787 268 1524 Fragments arranged by size: 1524 881 870 841 787 660 597 393 268 256 100 EcoO109I rG'GnC_Cy Cuts at: 2191 2248 2752 2790 3499 3976 3992 2191 Size: 57 504 38 709 477 16 5376 Fragments arranged by size: 5376 709 504 477 57 38 16 EcoRI G'AATT_C Cuts at: 4486 4864 4486 Size: 378 6799 EcoRV GAT'ATC Cuts at: 4183 4183 Size: 7177 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2477 2477 Size: 7177 FspI TGC'GCA Cuts at: 5683 5683 Size: 7177 HaeII r_GCGC'y Cuts at: 1678 3585 4109 4135 4153 6324 1678 Size: 1907 524 26 18 2171 2531 Fragments arranged by size: 2531 2171 1907 524 26 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2209 2241 2357 2389 2754 Size: 32 135 32 637 32 116 32 365 Cuts at: 2754 2786 3863 3895 4196 4198 4228 4230 4238 Size: 32 1077 32 301 2 30 2 8 Cuts at: 4238 4270 5818 5850 5892 5924 1373 Size: 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 1077 637 365 301 135 116 42 32 32 32 32 32 32 32 32 32 30 8 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 2109 2167 2811 3136 4521 4767 Size: 988 281 171 58 644 325 1385 246 Cuts at: 4767 669 Size: 3079 Fragments arranged by size: 3079 1385 988 644 325 281 246 171 58 HindIII A'AGCT_T Cuts at: 748 748 Size: 7177 HpaI GTT'AAC Cuts at: 2109 4767 2109 Size: 2658 4519 KasI G'GCGC_C Cuts at: 4131 4149 4131 Size: 18 7159 KpnI G_GTAC'C Cuts at: 4502 4502 Size: 7177 MfeI C'AATT_G Cuts at: 4776 4776 Size: 7177 MluI A'CGCG_T Cuts at: 7053 7053 Size: 7177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3246 3774 6291 6475 1305 Size: 1941 528 2517 184 2007 Fragments arranged by size: 2517 2007 1941 528 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3386 3922 10 Size: 54 1425 343 1554 536 3265 Fragments arranged by size: 3265 1554 1425 536 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 2477 2716 2960 3492 3521 4248 Size: 648 1198 113 239 244 532 29 727 Cuts at: 4248 4361 5135 5494 5653 518 Size: 113 774 359 159 2042 Fragments arranged by size: 2042 1198 774 727 648 532 359 244 239 159 113 113 29 MspA1I CmG'CkG Cuts at: 1606 2880 3471 4231 4593 5270 6418 6663 1606 Size: 1274 591 760 362 677 1148 245 2120 Fragments arranged by size: 2120 1274 1148 760 677 591 362 245 NaeI GCC'GGC Cuts at: 2901 2901 Size: 7177 NarI GG'CG_CC Cuts at: 4132 4150 4132 Size: 18 7159 NcoI C'CATG_G Cuts at: 513 1065 2539 3487 513 Size: 552 1474 948 4203 Fragments arranged by size: 4203 1474 948 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7177 NgoMIV G'CCGG_C Cuts at: 2899 2899 Size: 7177 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7177 NotI GC'GGCC_GC Cuts at: 4624 4624 Size: 7177 NspI r_CATG'y Cuts at: 1132 1229 2392 2947 4535 6080 1132 Size: 97 1163 555 1588 1545 2229 Fragments arranged by size: 2229 1588 1545 1163 555 97 PasI CC'CwG_GG Cuts at: 1348 2035 1348 Size: 687 6490 PciI A'CATG_T Cuts at: 6076 6076 Size: 7177 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 3370 3918 1108 Size: 1254 1008 548 4367 Fragments arranged by size: 4367 1254 1008 548 PfoI T'CCnGG_A Cuts at: 2309 2694 2309 Size: 385 6792 PmeI GTTT'AAAC Cuts at: 4481 4481 Size: 7177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5246 5278 6759 6791 5246 Size: 32 1481 32 5632 Fragments arranged by size: 5632 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2752 3499 3976 2248 Size: 504 747 477 5449 Fragments arranged by size: 5449 747 504 477 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7177 PsiI TTA'TAA Cuts at: 4747 4747 Size: 7177 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2972 3004 2972 Size: 32 7145 PstI C_TGCA'G Cuts at: 830 4221 4263 4529 830 Size: 3391 42 266 3478 Fragments arranged by size: 3478 3391 266 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7177 PvuII CAG'CTG Cuts at: 2880 3471 4231 2880 Size: 591 760 5826 Fragments arranged by size: 5826 760 591 SacI G_AGCT'C Cuts at: 3000 3543 3000 Size: 543 6634 SalI G'TCGA_C Cuts at: 1936 4519 1936 Size: 2583 4594 SanDI GG'GwC_CC Cuts at: 2248 2752 2248 Size: 504 6673 SapI GCTCTTCn'nnn_ Cuts at: 3548 3548 Size: 7177 SbfI CC_TGCA'GG Cuts at: 4529 4529 Size: 7177 ScaI AGT'ACT Cuts at: 2240 5425 7137 2240 Size: 3185 1712 2280 Fragments arranged by size: 3185 2280 1712 SfcI C'TryA_G Cuts at: 826 1045 4217 4259 4525 5660 6341 6532 826 Size: 219 3172 42 266 1135 681 191 1471 Fragments arranged by size: 3172 1471 1135 681 266 219 191 42 SfoI GGC'GCC Cuts at: 4133 4151 4133 Size: 18 7159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7177 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7177 SmaI CCC'GGG Cuts at: 1872 4504 1872 Size: 2632 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5281 6182 6444 820 Size: 197 926 3338 901 262 1553 Fragments arranged by size: 3338 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4474 4968 493 Size: 3981 494 2702 Fragments arranged by size: 3981 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7177 SphI G_CATG'C Cuts at: 1132 4535 1132 Size: 3403 3774 SspI AAT'ATT Cuts at: 5 52 5101 5 Size: 47 5049 2081 Fragments arranged by size: 5049 2081 47 StuI AGG'CCT Cuts at: 1517 3150 1517 Size: 1633 5544 StyI C'CwwG_G Cuts at: 513 1065 2539 3139 3487 4616 513 Size: 552 1474 600 348 1129 3074 Fragments arranged by size: 3074 1474 1129 600 552 348 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3358 3518 5204 5221 5374 5559 6004 Size: 1255 1778 160 1686 17 153 185 445 Cuts at: 6004 325 Size: 1498 Fragments arranged by size: 1778 1686 1498 1255 445 185 160 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2907 3291 Size: 274 80 33 51 1443 261 669 384 Cuts at: 3291 4110 4433 5423 7135 96 Size: 819 323 990 1712 138 Fragments arranged by size: 1712 1443 990 819 669 384 323 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3844 3876 4351 4383 4439 4471 7137 7169 3844 Size: 32 475 32 56 32 2666 32 3852 Fragments arranged by size: 3852 2666 475 56 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2815 2930 1316 Size: 1499 115 5563 Fragments arranged by size: 5563 1499 115 XbaI T'CTAG_A Cuts at: 4513 4513 Size: 7177 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2218 Size: 7177 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7177 XmaI C'CCGG_G Cuts at: 1870 4502 1870 Size: 2632 4545 XmnI GAAnn'nnTTC Cuts at: 3801 5306 3801 Size: 1505 5672 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3064 3571 4985 276 Size: 53 83 186 936 1530 507 1414 2468 Fragments arranged by size: 2468 1530 1414 936 507 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoNI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SexAI SfiI SrfI SwaI