Restriction map for
pFN21ASDA1260
(Linear) (Six-base) MAPSORT of: KIAA1260.seq Check: 5916 from: 1 to: 2448
KIAA1260.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2272 2448
Size: 2272 176
AatII G_ACGT'C
Cuts at: 0 1043 1550 2448
Size: 1043 507 898
Fragments arranged by size:
1043 898 507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1250 1506 2376 2448
Size: 1150 100 256 870 72
Fragments arranged by size:
1150 870 256 100 72
AgeI A'CCGG_T
Cuts at: 0 637 2448
Size: 637 1811
AleI CACnn'nnGTG
Cuts at: 0 693 2225 2448
Size: 693 1532 223
Fragments arranged by size:
1532 693 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1618 1650 2448
Size: 1618 32 798
Fragments arranged by size:
1618 798 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 339 1065 1972 2448
Size: 18 321 726 907 476
Fragments arranged by size:
907 726 476 321 18
ApoI r'AATT_y
Cuts at: 0 2415 2448
Size: 2415 33
AvaI C'yCGr_G
Cuts at: 0 756 2448
Size: 756 1692
BanI G'GyrC_C
Cuts at: 0 538 841 1870 2108 2126 2448
Size: 538 303 1029 238 18 322
Fragments arranged by size:
1029 538 322 303 238 18
BanII G_rGCy'C
Cuts at: 0 671 977 1520 2448
Size: 671 306 543 928
Fragments arranged by size:
928 671 543 306
BbeI G_GCGC'C
Cuts at: 0 2112 2130 2448
Size: 2112 18 318
Fragments arranged by size:
2112 318 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 437 1479 2448
Size: 437 1042 969
Fragments arranged by size:
1042 969 437
BciVI GTATCCnnnnn_n'
Cuts at: 0 143 390 1620 2448
Size: 143 247 1230 828
Fragments arranged by size:
1230 828 247 143
BclI T'GATC_A
Cuts at: 0 480 593 2448
Size: 480 113 1855
Fragments arranged by size:
1855 480 113
BglI GCCn_nnn'nGGC
Cuts at: 0 2044 2448
Size: 2044 404
BglII A'GATC_T
Cuts at: 0 194 2448
Size: 194 2254
BlpI GC'TnA_GC
Cuts at: 0 1555 2448
Size: 1555 893
Bme1580I G_kGCm'C
Cuts at: 0 331 461 541 2448
Size: 331 130 80 1907
Fragments arranged by size:
1907 331 130 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 1287 2126 2448
Size: 1287 839 322
Fragments arranged by size:
1287 839 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 2448
Size: 269 2179
BsaI GGTCTCn'nnnn_
Cuts at: 0 817 2448
Size: 817 1631
BsaAI yAC'GTr
Cuts at: 0 456 2448
Size: 456 1992
BsaBI GATnn'nnATC
Cuts at: 0 1607 2448
Size: 1607 841
BsaHI Gr'CG_yC
Cuts at: 0 1040 1109 1171 1547 2047 2109 2127 2323
Size: 1040 69 62 376 500 62 18 196
Cuts at: 2323 2448
Size: 125
Fragments arranged by size:
1040 500 376 196 125 69 62 62
18
BsaWI w'CCGG_w
Cuts at: 0 637 2448
Size: 637 1811
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 718 2044 2448
Size: 718 1326 404
Fragments arranged by size:
1326 718 404
BseYI C'CCAG_C
Cuts at: 0 332 763 857 1104 1444 1448 1914 2448
Size: 332 431 94 247 340 4 466 534
Fragments arranged by size:
534 466 431 340 332 247 94 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 2448
Size: 1360 1088
BsiEI CG_ry'CG
Cuts at: 0 2299 2448
Size: 2299 149
BsiHKAI G_wGCw'C
Cuts at: 0 977 1520 2448
Size: 977 543 928
Fragments arranged by size:
977 928 543
BsmI GAATG_Cn'
Cuts at: 0 952 2448
Size: 952 1496
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1294 2448
Size: 1294 1154
Bsp1286I G_dGCh'C
Cuts at: 0 331 461 541 671 977 1520 2448
Size: 331 130 80 130 306 543 928
Fragments arranged by size:
928 543 331 306 130 130 80
BspHI T'CATG_A
Cuts at: 0 477 1568 2448
Size: 477 1091 880
Fragments arranged by size:
1091 880 477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1382 2272 2448
Size: 1382 890 176
Fragments arranged by size:
1382 890 176
BsrBI CCG'CTC
Cuts at: 0 839 2448
Size: 839 1609
BsrDI GCAATG_nn'
Cuts at: 0 2033 2448
Size: 2033 415
BsrFI r'CCGG_y
Cuts at: 0 541 637 876 1023 2294 2448
Size: 541 96 239 147 1271 154
Fragments arranged by size:
1271 541 239 154 147 96
BsrGI T'GTAC_A
Cuts at: 0 1268 2448
Size: 1268 1180
BssSI C'ACGA_G
Cuts at: 0 2221 2448
Size: 2221 227
BstAPI GCAn_nnn'nTGC
Cuts at: 0 366 2373 2448
Size: 366 2007 75
Fragments arranged by size:
2007 366 75
BstEII G'GTnAC_C
Cuts at: 0 640 2448
Size: 640 1808
BstXI CCAn_nnnn'nTGG
Cuts at: 0 939 1206 2448
Size: 939 267 1242
Fragments arranged by size:
1242 939 267
BstYI r'GATC_y
Cuts at: 0 194 349 523 1061 2448
Size: 194 155 174 538 1387
Fragments arranged by size:
1387 538 194 174 155
Bsu36I CC'TnA_GG
Cuts at: 0 1622 1973 2448
Size: 1622 351 475
Fragments arranged by size:
1622 475 351
BtgI C'CryG_G
Cuts at: 0 461 516 932 1358 1464 1562 2424 2448
Size: 461 55 416 426 106 98 862 24
Fragments arranged by size:
862 461 426 416 106 98 55 24
BtsI GCAGTG_nn'
Cuts at: 0 688 869 1422 2448
Size: 688 181 553 1026
Fragments arranged by size:
1026 688 553 181
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1739 1774 2448
Size: 1739 35 674
Fragments arranged by size:
1739 674 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1984 2448
Size: 1039 945 464
Fragments arranged by size:
1039 945 464
EaeI y'GGCC_r
Cuts at: 0 878 998 1361 1897 2448
Size: 878 120 363 536 551
Fragments arranged by size:
878 551 536 363 120
EarI CTCTTCn'nnn_
Cuts at: 0 820 1525 2062 2174 2448
Size: 820 705 537 112 274
Fragments arranged by size:
820 705 537 274 112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1323 2448
Size: 1323 1125
EcoICRI GAG'CTC
Cuts at: 0 975 1518 2448
Size: 975 543 930
Fragments arranged by size:
975 930 543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1150 1250 1506 2376 2448
Size: 269 881 100 256 870 72
Fragments arranged by size:
881 870 269 256 100 72
EcoO109I rG'GnC_Cy
Cuts at: 0 168 225 729 767 1476 1953 1969 2448
Size: 168 57 504 38 709 477 16 479
Fragments arranged by size:
709 504 479 477 168 57 38 16
EcoRV GAT'ATC
Cuts at: 0 2160 2448
Size: 2160 288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 454 2448
Size: 454 1994
HaeII r_GCGC'y
Cuts at: 0 1562 2086 2112 2130 2448
Size: 1562 524 26 18 318
Fragments arranged by size:
1562 524 318 26 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 186 218 334 366 731 763 1840 1872
Size: 186 32 116 32 365 32 1077 32
Cuts at: 1872 2173 2175 2205 2207 2215 2247 2448
Size: 301 2 30 2 8 32 201
Fragments arranged by size:
1077 365 301 201 186 116 32 32
32 32 32 30 8 2 2
HincII GTy'rAC
Cuts at: 0 86 144 788 1113 2448
Size: 86 58 644 325 1335
Fragments arranged by size:
1335 644 325 86 58
HpaI GTT'AAC
Cuts at: 0 86 2448
Size: 86 2362
KasI G'GCGC_C
Cuts at: 0 2108 2126 2448
Size: 2108 18 322
Fragments arranged by size:
2108 322 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1223 1751 2448
Size: 1223 528 697
Fragments arranged by size:
1223 697 528
MscI TGG'CCA
Cuts at: 0 1363 1899 2448
Size: 1363 536 549
Fragments arranged by size:
1363 549 536
MslI CAynn'nnrTG
Cuts at: 0 341 454 693 937 1469 1498 2225 2338
Size: 341 113 239 244 532 29 727 113
Cuts at: 2338 2448
Size: 110
Fragments arranged by size:
727 532 341 244 239 113 113 110
29
MspA1I CmG'CkG
Cuts at: 0 857 1448 2208 2448
Size: 857 591 760 240
Fragments arranged by size:
857 760 591 240
NaeI GCC'GGC
Cuts at: 0 878 2448
Size: 878 1570
NarI GG'CG_CC
Cuts at: 0 2109 2127 2448
Size: 2109 18 321
Fragments arranged by size:
2109 321 18
NcoI C'CATG_G
Cuts at: 0 516 1464 2448
Size: 516 948 984
Fragments arranged by size:
984 948 516
NgoMIV G'CCGG_C
Cuts at: 0 876 2448
Size: 876 1572
NspI r_CATG'y
Cuts at: 0 369 924 2448
Size: 369 555 1524
Fragments arranged by size:
1524 555 369
PasI CC'CwG_GG
Cuts at: 0 12 2448
Size: 12 2436
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 1347 1895 2448
Size: 339 1008 548 553
Fragments arranged by size:
1008 553 548 339
PfoI T'CCnGG_A
Cuts at: 0 286 671 2448
Size: 286 385 1777
Fragments arranged by size:
1777 385 286
PpuMI rG'GwC_Cy
Cuts at: 0 225 729 1476 1953 2448
Size: 225 504 747 477 495
Fragments arranged by size:
747 504 495 477 225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 949 981 2448
Size: 949 32 1467
Fragments arranged by size:
1467 949 32
PstI C_TGCA'G
Cuts at: 0 2198 2240 2448
Size: 2198 42 208
Fragments arranged by size:
2198 208 42
PvuII CAG'CTG
Cuts at: 0 857 1448 2208 2448
Size: 857 591 760 240
Fragments arranged by size:
857 760 591 240
SacI G_AGCT'C
Cuts at: 0 977 1520 2448
Size: 977 543 928
Fragments arranged by size:
977 928 543
SanDI GG'GwC_CC
Cuts at: 0 225 729 2448
Size: 225 504 1719
Fragments arranged by size:
1719 504 225
SapI GCTCTTCn'nnn_
Cuts at: 0 1525 2448
Size: 1525 923
ScaI AGT'ACT
Cuts at: 0 217 2448
Size: 217 2231
SfcI C'TryA_G
Cuts at: 0 2194 2236 2448
Size: 2194 42 212
Fragments arranged by size:
2194 212 42
SfoI GGC'GCC
Cuts at: 0 2110 2128 2448
Size: 2110 18 320
Fragments arranged by size:
2110 320 18
StuI AGG'CCT
Cuts at: 0 1127 2448
Size: 1127 1321
StyI C'CwwG_G
Cuts at: 0 516 1116 1464 2448
Size: 516 600 348 984
Fragments arranged by size:
984 600 516 348
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1335 1495 2448
Size: 1335 160 953
Fragments arranged by size:
1335 953 160
TatI w'GTAC_w
Cuts at: 0 215 884 1268 2087 2410 2448
Size: 215 669 384 819 323 38
Fragments arranged by size:
819 669 384 323 215 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1821 1853 2328 2360 2416 2448
Size: 1821 32 475 32 56 32
Fragments arranged by size:
1821 475 56 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 792 907 2448
Size: 792 115 1541
Fragments arranged by size:
1541 792 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 2448
Size: 195 2253
XmnI GAAnn'nnTTC
Cuts at: 0 1778 2448
Size: 1778 670
ZraI GAC'GTC
Cuts at: 0 1041 1548 2448
Size: 1041 507 900
Fragments arranged by size:
1041 900 507
Enzymes that do cut:
AarI AatII AcuI AgeI AleI AloI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HpaI KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PsrI PstI PvuII SacI
SanDI SapI ScaI SfcI SfoI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BfrBI BmgBI BmtI BplI Bpu10I BpuEI
BsaXI BsiWI BspEI BssHII BstBI BstZ17I BtgZI ClaI
DraI DraIII EagI EcoNI EcoRI FseI FspI FspAI
HindIII KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SbfI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmaI