Restriction map for
pFN21ASDA1229
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
HphI KasI KpnI KspI MaeII
MluI MroI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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BalI 1724 TGGCCA TGG^CCA
BsaBI 1522 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1478 GTGCAG GTGCAG(16/14)
BspMI 653 ACCTGC ACCTGC(4/8)
BssSI 1438 CACGAG CACGAG(-5/-1)
BstF5I 1164 GGATG GGATG(2/0)
Cac8I 1650 GCTGGC GC[ACGT]^[ACGT]GC
EaeI 1724 TGGCCA [CT]^GGCC[GA]
EcoNI 114 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 963 CCATGG C^C[AT][AT]GG
FokI 1164 GGATG GGATG(9/13)
HgaI 1621 GACGC GACGC(5/10)
MaeIII 178 GTAAC ^GT[ACGT]AC
MamI 1522 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1007 CAATTG C^AATTG
MluNI 1724 TGGCCA TGG^CCA
MscI 1724 TGGCCA TGG^CCA
MslI 1015 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1007 CAATTG C^AATTG
MwoI 838 GCTTCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 963 CCATGG C^CATGG
NlaIV 650 GGAACC GG[ACGT]^[ACGT]CC
NspI 392 GCATGT [GA]CATG^[CT]
PflMI 842 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1858 GAAGAGC GCTCTTC(1/4)
SfaNI 733 GCATC GCATC(5/9)
StyI 963 CCATGG C^C[AT][AT]GG
Van91I 842 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1795 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 570 GATCC GGATC(4/5)
1894 GATCC
BbsI 958 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BglII 942 AGATCT A^GATCT
1827 AGATCT
BpuAI 958 GAAGAC GAAGAC(2/6)
1757 GAAGAC
BsaJI 82 CCACGG C^C[ACGT][ACGT]GG
963 CCATGG
BsiYI 114 CCTGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
842 CCAATGCCTGG
BslI 114 CCTGAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
842 CCAATGCCTGG
BsrI 1734 ACTGG ACTGG(1/-1)
92 CCAGT
BstXI 1515 CCAGGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCAGACAACTGG
Cfr13I 465 GGCCC G^G[ACGT]CC
1512 GGGCC
DraI 723 TTTAAA TTT^AAA
1693 TTTAAA
DsaI 82 CCACGG C^C[GA][CT]GG
963 CCATGG
EarI 1558 GAAGAG CTCTTC(1/4)
1858 GAAGAG
Ksp632I 1558 GAAGAG CTCTTC(1/4)
1858 GAAGAG
PstI 1456 CTGCAG CTGCA^G
1706 CTGCAG
Sau96I 465 GGCCC G^G[ACGT]CC
1512 GGGCC
SspI 291 AATATT AAT^ATT
1821 AATATT
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
ApoI AseI AsnI Asp700I BbvI
BfaI BfrI BsmAI BssKI BstNI
BstYI DdeI DpnII EcoRII Fnu4HI
HaeIII HindIII HinfI ItaI MaeI
MboI MboII MflI MnlI MseI
MspA1I MvaI NdeII NlaIII PleI
PshBI PvuII RsaI Sau3AI ScrFI
SfcI SmlI TfiI Tru9I TseI
Tsp509I TspRI XhoII XmnI