(Circular) (Six-base) MAPSORT of: pFN21ASDA1229.seq Check: 6042 from: 1 to: 6637 pFN21ASDA1229 6637 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4447 278 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3980 1937 Size: 2043 4594 Acc65I G'GTAC_C Cuts at: 3958 3958 Size: 6637 AclI AA'CG_TT Cuts at: 1563 4764 5137 1563 Size: 3201 373 3063 Fragments arranged by size: 3201 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 2990 3428 3515 3626 3816 4700 6084 Size: 527 831 438 87 111 190 884 1384 Cuts at: 6084 6352 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 884 831 527 438 268 190 111 87 AflII C'TTAA_G Cuts at: 820 1017 2183 2462 3695 3710 820 Size: 197 1166 279 1233 15 3747 Fragments arranged by size: 3747 1233 1166 279 197 15 AflIII A'CryG_T Cuts at: 1203 5536 6513 1203 Size: 4333 977 1327 Fragments arranged by size: 4333 1327 977 AgeI A'CCGG_T Cuts at: 5468 5468 Size: 6637 AhdI GACnn_n'nnGTC Cuts at: 5366 5366 Size: 6637 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6637 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6637 AlwNI CAG_nnn'CTG Cuts at: 4380 5952 4380 Size: 1572 5065 ApaLI G'TGCA_C Cuts at: 4694 5850 4694 Size: 1156 5481 ApoI r'AATT_y Cuts at: 1493 2287 2598 2995 3009 3325 3946 4172 4324 Size: 794 311 397 14 316 621 226 152 Cuts at: 4324 6433 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 794 621 397 316 311 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 2256 2318 2952 5191 160 Size: 872 1224 62 634 2239 1606 Fragments arranged by size: 2239 1606 1224 872 634 62 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 3962 1870 Size: 73 2019 4545 Fragments arranged by size: 4545 2019 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3269 3302 3269 Size: 33 6604 BamHI G'GATC_C Cuts at: 3967 4334 3967 Size: 367 6270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3958 5413 618 Size: 325 217 627 2171 1455 1842 Fragments arranged by size: 2171 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3956 1391 Size: 2565 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2988 3787 928 Size: 971 1089 799 3778 Fragments arranged by size: 3778 1089 971 799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4828 4862 1903 Size: 34 2891 34 3678 Fragments arranged by size: 3678 2891 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4529 5739 4529 Size: 1210 5427 BclI T'GATC_A Cuts at: 1548 1548 Size: 6637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5248 136 Size: 107 122 71 631 605 3576 1525 Fragments arranged by size: 3576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2965 3850 6632 2965 Size: 885 2782 2970 Fragments arranged by size: 2970 2782 885 BlpI GC'TnA_GC Cuts at: 4054 4054 Size: 6637 Bme1580I G_kGCm'C Cuts at: 1790 4698 5854 1790 Size: 2908 1156 2573 Fragments arranged by size: 2908 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4368 5326 442 Size: 416 53 83 399 24 2951 958 1753 Fragments arranged by size: 2951 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5297 1239 Size: 4058 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3080 3683 4762 5627 5925 3080 Size: 603 1079 865 298 3792 Fragments arranged by size: 3792 1079 865 603 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5300 882 Size: 617 3801 2219 Fragments arranged by size: 3801 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3934 4428 493 Size: 711 2730 494 2702 Fragments arranged by size: 2730 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3549 6631 3549 Size: 3082 3555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4444 4826 5461 275 Size: 53 83 186 936 2911 382 635 1451 Fragments arranged by size: 2911 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5070 5468 5742 5889 6300 5070 Size: 398 274 147 411 5407 Fragments arranged by size: 5407 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6412 Fragments arranged by size: 6412 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6637 BseYI C'CCAG_C Cuts at: 5840 6336 5840 Size: 496 6141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3522 1163 Size: 573 1786 4278 Fragments arranged by size: 4278 1786 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4087 4848 5876 1540 Size: 275 186 2086 761 1028 2301 Fragments arranged by size: 2301 2086 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4698 4783 5854 4698 Size: 85 1071 5481 Fragments arranged by size: 5481 1071 85 BsmI GAATG_Cn' Cuts at: 4148 4241 4148 Size: 93 6544 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3956 4698 4783 5854 1391 Size: 399 2166 742 85 1071 2174 Fragments arranged by size: 2174 2166 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6300 6300 Size: 6637 BspHI T'CATG_A Cuts at: 4526 6256 4526 Size: 1730 4907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2685 3992 844 Size: 1841 1307 3489 Fragments arranged by size: 3489 1841 1307 BsrBI CCG'CTC Cuts at: 1394 1823 4524 1394 Size: 429 2701 3507 Fragments arranged by size: 3507 2701 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5132 5306 65 Size: 1183 3884 174 1396 Fragments arranged by size: 3884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5281 5468 1700 Size: 110 3471 187 2869 Fragments arranged by size: 3471 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6637 BssSI C'ACGA_G Cuts at: 3461 4697 5709 3461 Size: 1236 1012 4389 Fragments arranged by size: 4389 1236 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6637 BstBI TT'CG_AA Cuts at: 2007 5441 2007 Size: 3434 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6637 BstXI CCAn_nnnn'nTGG Cuts at: 3545 3757 3545 Size: 212 6425 BstYI r'GATC_y Cuts at: 1972 2592 2965 3850 3967 4334 4719 4736 6188 Size: 620 373 885 117 367 385 17 1452 Cuts at: 6188 6274 6286 6632 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 885 620 385 373 367 346 117 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2105 2986 513 Size: 552 957 83 881 4164 Fragments arranged by size: 4164 957 881 552 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5898 Fragments arranged by size: 5898 683 56 BtsI GCAGTG_nn' Cuts at: 3488 4153 4947 4967 3488 Size: 665 794 20 5158 Fragments arranged by size: 5158 794 665 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6637 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6602 DraI TTT'AAA Cuts at: 2748 3718 3941 2748 Size: 970 223 5444 Fragments arranged by size: 5444 970 223 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5644 809 Size: 507 4328 1802 Fragments arranged by size: 4328 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3747 4084 4973 Size: 54 1425 50 275 18 1917 337 889 Cuts at: 4973 6602 8 Size: 1629 43 Fragments arranged by size: 1917 1629 1425 889 337 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4084 1537 Size: 275 2272 4090 Fragments arranged by size: 4090 2272 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3576 3876 4567 1353 Size: 2223 300 691 3423 Fragments arranged by size: 3423 2223 691 300 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3461 5224 5598 5744 387 Size: 1064 138 1872 1763 374 146 1280 Fragments arranged by size: 1872 1763 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2990 3428 3515 3626 3816 4700 Size: 393 527 831 438 87 111 190 884 Cuts at: 4700 5297 6084 6352 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 884 831 787 597 527 438 393 268 190 111 87 EcoNI CCTnn'n_nnAGG Cuts at: 2141 2141 Size: 6637 EcoRI G'AATT_C Cuts at: 3946 4324 3946 Size: 378 6259 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2198 3029 3710 2198 Size: 831 681 5125 Fragments arranged by size: 5125 831 681 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 5784 1678 Size: 4106 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2393 2425 2577 2609 3385 Size: 32 135 32 821 32 152 32 776 Cuts at: 3385 3417 3797 3829 5278 5310 5352 5384 1373 Size: 32 380 32 1449 32 42 32 2626 Fragments arranged by size: 2626 1449 821 776 380 152 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3981 4227 669 Size: 988 281 2043 246 3079 Fragments arranged by size: 3079 2043 988 281 246 HindIII A'AGCT_T Cuts at: 748 2807 3077 3569 748 Size: 2059 270 492 3816 Fragments arranged by size: 3816 2059 492 270 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KpnI G_GTAC'C Cuts at: 3962 3962 Size: 6637 MfeI C'AATT_G Cuts at: 3030 4236 3030 Size: 1206 5431 MluI A'CGCG_T Cuts at: 6513 6513 Size: 6637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5751 5935 1305 Size: 4446 184 2007 Fragments arranged by size: 4446 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3749 10 Size: 54 1425 343 1917 2898 Fragments arranged by size: 2898 1917 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3042 4595 4954 5113 518 Size: 648 1876 1553 359 159 2042 Fragments arranged by size: 2042 1876 1553 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 3299 3604 4053 4730 5878 6123 1606 Size: 527 1166 305 449 677 1148 245 2120 Fragments arranged by size: 2120 1166 1148 677 527 449 305 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2986 513 Size: 552 957 964 4164 Fragments arranged by size: 4164 964 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 2419 3995 5540 1132 Size: 97 1190 1576 1545 2229 Fragments arranged by size: 2229 1576 1545 1190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6637 PciI A'CATG_T Cuts at: 5536 5536 Size: 6637 PflMI CCAn_nnn'nTGG Cuts at: 1108 2871 1108 Size: 1763 4874 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4706 4738 6219 6251 4706 Size: 32 1481 32 5092 Fragments arranged by size: 5092 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 4207 4207 Size: 6637 PstI C_TGCA'G Cuts at: 830 3483 3733 3989 830 Size: 2653 250 256 3478 Fragments arranged by size: 3478 2653 256 250 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 PvuII CAG'CTG Cuts at: 2133 3299 3604 2133 Size: 1166 305 5166 Fragments arranged by size: 5166 1166 305 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SapI GCTCTTCn'nnn_ Cuts at: 3876 3876 Size: 6637 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 4885 6597 4885 Size: 1712 4925 SfcI C'TryA_G Cuts at: 826 1045 2211 2588 2706 2739 3479 3729 3985 Size: 219 1166 377 118 33 740 250 256 Cuts at: 3985 5120 5801 5992 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1166 1135 740 681 377 256 250 219 191 118 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6637 SmaI CCC'GGG Cuts at: 1872 3964 1872 Size: 2092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 2462 3059 3662 3695 3710 Size: 197 926 240 279 597 603 33 15 Cuts at: 3710 4741 5642 5904 820 Size: 1031 901 262 1553 Fragments arranged by size: 1553 1031 926 901 603 597 279 262 240 197 33 15 SnaBI TAC'GTA Cuts at: 493 3934 4428 493 Size: 3441 494 2702 Fragments arranged by size: 3441 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6637 SphI G_CATG'C Cuts at: 1132 3995 1132 Size: 2863 3774 SspI AAT'ATT Cuts at: 5 52 2316 3846 4561 5 Size: 47 2264 1530 715 2081 Fragments arranged by size: 2264 2081 1530 715 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6637 StyI C'CwwG_G Cuts at: 513 1065 2022 2986 4076 513 Size: 552 957 964 1090 3074 Fragments arranged by size: 3074 1090 964 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4664 4681 4834 5019 5464 325 Size: 1255 3084 17 153 185 445 1498 Fragments arranged by size: 3084 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2575 2920 4883 Size: 274 80 33 51 1443 598 345 1963 Cuts at: 4883 6595 96 Size: 1712 138 Fragments arranged by size: 1963 1712 1443 598 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6597 6629 6597 Size: 32 6605 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3818 3973 3818 Size: 155 6482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6637 XmaI C'CCGG_G Cuts at: 1870 3962 1870 Size: 2092 4545 XmnI GAAnn'nnTTC Cuts at: 2050 2367 2995 3009 3618 4766 2050 Size: 317 628 14 609 1148 3921 Fragments arranged by size: 3921 1148 628 609 317 14 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4445 276 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI