Restriction map for
pFN21ASDA1229
(Linear) (Six-base) MAPSORT of: KIAA1229.seq Check: 2353 from: 1 to: 1908
KIAA1229.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 967 1405 1492 1603 1793 1908
Size: 136 831 438 87 111 190 115
Fragments arranged by size:
831 438 190 136 115 111 87
AflII C'TTAA_G
Cuts at: 0 160 439 1672 1687 1908
Size: 160 279 1233 15 221
Fragments arranged by size:
1233 279 221 160 15
ApoI r'AATT_y
Cuts at: 0 264 575 972 986 1302 1908
Size: 264 311 397 14 316 606
Fragments arranged by size:
606 397 316 311 264 14
AseI AT'TA_AT
Cuts at: 0 233 295 929 1908
Size: 233 62 634 979
Fragments arranged by size:
979 634 233 62
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1246 1279 1908
Size: 1246 33 629
Fragments arranged by size:
1246 629 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 1764 1908
Size: 965 799 144
Fragments arranged by size:
965 799 144
BglII A'GATC_T
Cuts at: 0 942 1827 1908
Size: 942 885 81
Fragments arranged by size:
942 885 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1660 1908
Size: 1057 603 248
Fragments arranged by size:
1057 603 248
BsaBI GATnn'nnATC
Cuts at: 0 1526 1908
Size: 1526 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 1908
Size: 1499 409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 1908
Size: 662 1246
BssSI C'ACGA_G
Cuts at: 0 1438 1908
Size: 1438 470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1522 1734 1908
Size: 1522 212 174
Fragments arranged by size:
1522 212 174
BstYI r'GATC_y
Cuts at: 0 569 942 1827 1908
Size: 569 373 885 81
Fragments arranged by size:
885 569 373 81
BtgI C'CryG_G
Cuts at: 0 82 963 1908
Size: 82 881 945
Fragments arranged by size:
945 881 82
BtsI GCAGTG_nn'
Cuts at: 0 1465 1908
Size: 1465 443
DraI TTT'AAA
Cuts at: 0 725 1695 1908
Size: 725 970 213
Fragments arranged by size:
970 725 213
EaeI y'GGCC_r
Cuts at: 0 1724 1908
Size: 1724 184
EarI CTCTTCn'nnn_
Cuts at: 0 1553 1853 1908
Size: 1553 300 55
Fragments arranged by size:
1553 300 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1438 1908
Size: 1438 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 967 1405 1492 1603 1793 1908
Size: 136 831 438 87 111 190 115
Fragments arranged by size:
831 438 190 136 115 111 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 118 1908
Size: 118 1790
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 1006 1687 1908
Size: 175 831 681 221
Fragments arranged by size:
831 681 221 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 554 586 1362 1394 1774 1806
Size: 370 32 152 32 776 32 380 32
Cuts at: 1806 1908
Size: 102
Fragments arranged by size:
776 380 370 152 102 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 784 1054 1546 1908
Size: 784 270 492 362
Fragments arranged by size:
784 492 362 270
MfeI C'AATT_G
Cuts at: 0 1007 1908
Size: 1007 901
MscI TGG'CCA
Cuts at: 0 1726 1908
Size: 1726 182
MslI CAynn'nnrTG
Cuts at: 0 1019 1908
Size: 1019 889
MspA1I CmG'CkG
Cuts at: 0 110 1276 1581 1908
Size: 110 1166 305 327
Fragments arranged by size:
1166 327 305 110
NcoI C'CATG_G
Cuts at: 0 963 1908
Size: 963 945
NspI r_CATG'y
Cuts at: 0 396 1908
Size: 396 1512
PflMI CCAn_nnn'nTGG
Cuts at: 0 848 1908
Size: 848 1060
PstI C_TGCA'G
Cuts at: 0 1460 1710 1908
Size: 1460 250 198
Fragments arranged by size:
1460 250 198
PvuII CAG'CTG
Cuts at: 0 110 1276 1581 1908
Size: 110 1166 305 327
Fragments arranged by size:
1166 327 305 110
SapI GCTCTTCn'nnn_
Cuts at: 0 1853 1908
Size: 1853 55
SfcI C'TryA_G
Cuts at: 0 188 565 683 716 1456 1706 1908
Size: 188 377 118 33 740 250 202
Fragments arranged by size:
740 377 250 202 188 118 33
SmlI C'TyrA_G
Cuts at: 0 160 439 1036 1639 1672 1687 1908
Size: 160 279 597 603 33 15 221
Fragments arranged by size:
603 597 279 221 160 33 15
SspI AAT'ATT
Cuts at: 0 293 1823 1908
Size: 293 1530 85
Fragments arranged by size:
1530 293 85
StyI C'CwwG_G
Cuts at: 0 963 1908
Size: 963 945
TatI w'GTAC_w
Cuts at: 0 552 897 1908
Size: 552 345 1011
Fragments arranged by size:
1011 552 345
XbaI T'CTAG_A
Cuts at: 0 1795 1908
Size: 1795 113
XmnI GAAnn'nnTTC
Cuts at: 0 27 344 972 986 1595 1908
Size: 27 317 628 14 609 313
Fragments arranged by size:
628 609 317 313 27 14
Enzymes that do cut:
AcuI AflII ApoI AseI BaeI BbsI BglII BpuEI
BsaBI BsgI BspMI BssSI BstXI BstYI BtgI BtsI
DraI EaeI EarI EciI Eco57MI EcoNI FalI Hin4I
HindIII MfeI MscI MslI MspA1I NcoI NspI PflMI
PstI PvuII SapI SfcI SmlI SspI StyI TatI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EcoICRI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TstI Tth111I XcmI XhoI
XmaI ZraI