Restriction map for
pFN21ASDA1194
(Linear) (Six-base) MAPSORT of: KIAA1194.seq Check: 6538 from: 1 to: 1671
KIAA1194.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 43 1671
Size: 43 1628
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 697 771 959 1329 1671
Size: 185 512 74 188 370 342
Fragments arranged by size:
512 370 342 188 185 74
AflII C'TTAA_G
Cuts at: 0 126 1671
Size: 126 1545
AflIII A'CryG_T
Cuts at: 0 834 1671
Size: 834 837
AlwNI CAG_nnn'CTG
Cuts at: 0 478 1255 1562 1671
Size: 478 777 307 109
Fragments arranged by size:
777 478 307 109
ApaI G_GGCC'C
Cuts at: 0 1522 1671
Size: 1522 149
ApoI r'AATT_y
Cuts at: 0 194 254 871 901 924 1201 1671
Size: 194 60 617 30 23 277 470
Fragments arranged by size:
617 470 277 194 60 30 23
BanII G_rGCy'C
Cuts at: 0 23 305 1522 1619 1671
Size: 23 282 1217 97 52
Fragments arranged by size:
1217 282 97 52 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 703 1671
Size: 47 656 968
Fragments arranged by size:
968 656 47
BbvCI CC'TCA_GC
Cuts at: 0 1493 1671
Size: 1493 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 1671
Size: 1002 34 635
Fragments arranged by size:
1002 635 34
BfrBI ATG'CAT
Cuts at: 0 1086 1671
Size: 1086 585
BglII A'GATC_T
Cuts at: 0 1233 1671
Size: 1233 438
Bme1580I G_kGCm'C
Cuts at: 0 1522 1671
Size: 1522 149
BmrI ACTGGGnnnn_n'
Cuts at: 0 99 657 1671
Size: 99 558 1014
Fragments arranged by size:
1014 558 99
BmtI G_CTAG'C
Cuts at: 0 916 1671
Size: 916 755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1671
Size: 1633 38
Bpu10I CC'TnA_GC
Cuts at: 0 1493 1671
Size: 1493 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 705 1671
Size: 705 966
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1306 1336 1671
Size: 1306 30 335
Fragments arranged by size:
1306 335 30
BseYI C'CCAG_C
Cuts at: 0 345 1671
Size: 345 1326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1671
Size: 1250 421
BsiHKAI G_wGCw'C
Cuts at: 0 305 1279 1619 1671
Size: 305 974 340 52
Fragments arranged by size:
974 340 305 52
Bsp1286I G_dGCh'C
Cuts at: 0 23 305 1279 1522 1619 1671
Size: 23 282 974 243 97 52
Fragments arranged by size:
974 282 243 97 52 23
BspHI T'CATG_A
Cuts at: 0 959 1671
Size: 959 712
BsrDI GCAATG_nn'
Cuts at: 0 206 699 1671
Size: 206 493 972
Fragments arranged by size:
972 493 206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 518 1194 1660 1671
Size: 518 676 466 11
Fragments arranged by size:
676 518 466 11
BstYI r'GATC_y
Cuts at: 0 1233 1671
Size: 1233 438
BstZ17I GTA'TAC
Cuts at: 0 44 1671
Size: 44 1627
Bsu36I CC'TnA_GG
Cuts at: 0 1506 1671
Size: 1506 165
BtgI C'CryG_G
Cuts at: 0 917 1671
Size: 917 754
ClaI AT'CG_AT
Cuts at: 0 1012 1671
Size: 1012 659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1069 1104 1671
Size: 1069 35 567
Fragments arranged by size:
1069 567 35
DraI TTT'AAA
Cuts at: 0 311 1671
Size: 311 1360
EcoICRI GAG'CTC
Cuts at: 0 303 1617 1671
Size: 303 1314 54
Fragments arranged by size:
1314 303 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 697 771 959 1329 1633 1671
Size: 185 512 74 188 370 304 38
Fragments arranged by size:
512 370 304 188 185 74 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 418 1671
Size: 397 21 1253
Fragments arranged by size:
1253 397 21
EcoO109I rG'GnC_Cy
Cuts at: 0 1092 1519 1671
Size: 1092 427 152
Fragments arranged by size:
1092 427 152
EcoRI G'AATT_C
Cuts at: 0 194 1671
Size: 194 1477
EcoRV GAT'ATC
Cuts at: 0 702 1671
Size: 702 969
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 152 1671
Size: 152 1519
HaeII r_GCGC'y
Cuts at: 0 643 1671
Size: 643 1028
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 454 486 1153 1185 1579 1611
Size: 229 32 193 32 667 32 394 32
Cuts at: 1611 1671
Size: 60
Fragments arranged by size:
667 394 229 193 60 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1644 1671
Size: 1644 27
HindIII A'AGCT_T
Cuts at: 0 123 214 1671
Size: 123 91 1457
Fragments arranged by size:
1457 123 91
MslI CAynn'nnrTG
Cuts at: 0 138 173 839 1671
Size: 138 35 666 832
Fragments arranged by size:
832 666 138 35
MspA1I CmG'CkG
Cuts at: 0 1349 1497 1671
Size: 1349 148 174
Fragments arranged by size:
1349 174 148
NcoI C'CATG_G
Cuts at: 0 917 1671
Size: 917 754
NheI G'CTAG_C
Cuts at: 0 912 1671
Size: 912 759
NsiI A_TGCA'T
Cuts at: 0 1088 1671
Size: 1088 583
NspI r_CATG'y
Cuts at: 0 838 1086 1671
Size: 838 248 585
Fragments arranged by size:
838 585 248
PciI A'CATG_T
Cuts at: 0 834 1671
Size: 834 837
PflMI CCAn_nnn'nTGG
Cuts at: 0 1042 1255 1539 1671
Size: 1042 213 284 132
Fragments arranged by size:
1042 284 213 132
PpuMI rG'GwC_Cy
Cuts at: 0 1092 1671
Size: 1092 579
PspOMI G'GGCC_C
Cuts at: 0 1518 1671
Size: 1518 153
PvuII CAG'CTG
Cuts at: 0 1349 1497 1671
Size: 1349 148 174
Fragments arranged by size:
1349 174 148
SacI G_AGCT'C
Cuts at: 0 305 1619 1671
Size: 305 1314 52
Fragments arranged by size:
1314 305 52
SanDI GG'GwC_CC
Cuts at: 0 1092 1671
Size: 1092 579
ScaI AGT'ACT
Cuts at: 0 989 1671
Size: 989 682
SfcI C'TryA_G
Cuts at: 0 654 1671
Size: 654 1017
SmlI C'TyrA_G
Cuts at: 0 126 684 1671
Size: 126 558 987
Fragments arranged by size:
987 558 126
SspI AAT'ATT
Cuts at: 0 854 1270 1671
Size: 854 416 401
Fragments arranged by size:
854 416 401
StuI AGG'CCT
Cuts at: 0 377 1671
Size: 377 1294
StyI C'CwwG_G
Cuts at: 0 511 917 1671
Size: 511 406 754
Fragments arranged by size:
754 511 406
SwaI ATTT'AAAT
Cuts at: 0 311 1671
Size: 311 1360
TatI w'GTAC_w
Cuts at: 0 987 1671
Size: 987 684
Tth111I GACn'n_nGTC
Cuts at: 0 813 1671
Size: 813 858
XbaI T'CTAG_A
Cuts at: 0 799 1671
Size: 799 872
XmnI GAAnn'nnTTC
Cuts at: 0 198 1671
Size: 198 1473
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApaI ApoI BanII
BbsI BbvCI BcgI BfrBI BglII Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BspHI BsrDI BstXI BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII MslI MspA1I NcoI
NheI NsiI NspI PciI PflMI PpuMI PspOMI PvuII
SacI SanDI ScaI SfcI SmlI SspI StuI StyI
SwaI TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BciVI BclI BglI BlpI
BmgBI BplI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI TaqII TstI XcmI XhoI XmaI ZraI