(Circular) (Six-base) MAPSORT of: pFN21ASDA1142.seq Check: 9224 from: 1 to: 6043 pFN21ASDA1142 6043 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2184 3051 3386 1937 Size: 247 867 335 4594 Fragments arranged by size: 4594 867 335 247 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3188 3218 3284 4106 5490 5758 1632 Size: 1556 30 66 822 1384 268 1917 Fragments arranged by size: 1917 1556 1384 822 268 66 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 3058 4772 3058 Size: 1714 4329 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2984 1184 Size: 1800 4243 AlwNI CAG_nnn'CTG Cuts at: 2146 2845 3786 5358 2146 Size: 699 941 1572 2831 Fragments arranged by size: 2831 1572 941 699 ApaI G_GGCC'C Cuts at: 2221 2421 2221 Size: 200 5843 ApaLI G'TGCA_C Cuts at: 2084 4100 5256 2084 Size: 2016 1156 2871 Fragments arranged by size: 2871 2016 1156 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2316 2322 2590 3368 1870 Size: 73 270 103 6 268 778 4545 Fragments arranged by size: 4545 778 270 268 103 73 6 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2765 3364 4819 618 Size: 325 217 627 540 438 599 1455 1842 Fragments arranged by size: 1842 1455 627 599 540 438 325 217 BanII G_rGCy'C Cuts at: 1391 2221 2323 2370 2421 2555 2736 3021 3102 Size: 830 102 47 51 134 181 285 81 Cuts at: 3102 3362 1391 Size: 260 4072 Fragments arranged by size: 4072 830 285 260 181 134 102 81 51 47 BbeI G_GCGC'C Cuts at: 2769 2769 Size: 6043 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2470 3935 5145 2470 Size: 1465 1210 3368 Fragments arranged by size: 3368 1465 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3242 4654 136 Size: 107 122 71 631 605 1570 1412 1525 Fragments arranged by size: 1570 1525 1412 631 605 122 107 71 BglII A'GATC_T Cuts at: 2052 6038 2052 Size: 3986 2057 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2088 2221 2332 2421 2973 3312 4104 5260 Size: 298 133 111 89 552 339 792 1156 Cuts at: 5260 1790 Size: 2573 Fragments arranged by size: 2573 1156 792 552 339 298 133 111 89 BmgBI CAC'GTC Cuts at: 1318 2242 1318 Size: 924 5119 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2278 3774 4732 Size: 416 53 83 399 24 861 1496 958 Cuts at: 4732 442 Size: 1753 Fragments arranged by size: 1753 1496 958 861 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2786 3040 2786 Size: 254 5789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2361 2367 4703 1239 Size: 1122 6 2336 2579 Fragments arranged by size: 2579 2336 1122 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3222 4706 882 Size: 617 1723 1484 2219 Fragments arranged by size: 2219 1723 1484 617 BsaAI yAC'GTr Cuts at: 493 1204 3340 3834 493 Size: 711 2136 494 2702 Fragments arranged by size: 2702 2136 711 494 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2766 2868 3850 4232 Size: 53 83 186 936 1233 102 982 382 Cuts at: 4232 4867 275 Size: 635 1451 Fragments arranged by size: 1451 1233 982 936 635 382 186 102 83 53 BsaWI w'CCGG_w Cuts at: 4476 4874 5148 5295 5706 4476 Size: 398 274 147 411 4813 Fragments arranged by size: 4813 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2501 2531 1178 Size: 30 165 30 1098 30 4690 Fragments arranged by size: 4690 1098 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2281 2284 2287 2290 2819 1186 Size: 982 113 3 3 3 529 4410 Fragments arranged by size: 4410 982 529 113 3 3 3 BseYI C'CCAG_C Cuts at: 2333 2347 2370 3064 3237 5246 5742 2333 Size: 14 23 694 173 2009 496 2634 Fragments arranged by size: 2634 2009 694 496 173 23 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2374 3161 1163 Size: 573 638 787 4045 Fragments arranged by size: 4045 787 638 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2820 3493 4254 5282 1540 Size: 275 186 819 673 761 1028 2301 Fragments arranged by size: 2301 1028 819 761 673 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2088 2736 2856 3021 4104 4189 5260 2079 Size: 9 648 120 165 1083 85 1071 2862 Fragments arranged by size: 2862 1083 1071 648 165 120 85 9 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2088 2221 2323 2332 2370 2421 Size: 399 289 9 133 102 9 38 51 Cuts at: 2421 2555 2736 2856 2973 3021 3102 3312 3362 Size: 134 181 120 117 48 81 210 50 Cuts at: 3362 4104 4189 5260 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 289 210 181 134 133 120 117 102 85 81 51 50 48 38 9 9 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 2236 2670 3932 5662 2236 Size: 434 1262 1730 2617 Fragments arranged by size: 2617 1730 1262 434 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2631 3162 3183 3398 844 Size: 1787 531 21 215 3489 Fragments arranged by size: 3489 1787 531 215 21 BsrBI CCG'CTC Cuts at: 1394 1823 2433 2517 3930 1394 Size: 429 610 84 1413 3507 Fragments arranged by size: 3507 1413 610 429 84 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2166 4687 4874 1700 Size: 110 356 2521 187 2869 Fragments arranged by size: 2869 2521 356 187 110 BsrGI T'GTAC_A Cuts at: 96 2706 96 Size: 2610 3433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6043 BssSI C'ACGA_G Cuts at: 2105 2687 4103 5115 2105 Size: 582 1416 1012 3033 Fragments arranged by size: 3033 1416 1012 582 BstAPI GCAn_nnn'nTGC Cuts at: 794 2083 2568 794 Size: 1289 485 4269 Fragments arranged by size: 4269 1289 485 BstBI TT'CG_AA Cuts at: 2007 4847 2007 Size: 2840 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2052 3373 3740 4125 4142 5594 5680 5692 Size: 80 1321 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1321 385 367 346 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 2556 3246 513 Size: 552 1491 690 3310 Fragments arranged by size: 3310 1491 690 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2557 487 Size: 683 56 1331 3973 Fragments arranged by size: 3973 1331 683 56 BtsI GCAGTG_nn' Cuts at: 2838 3559 4353 4373 2838 Size: 721 794 20 4508 Fragments arranged by size: 4508 794 721 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DraIII CAC_nnn'GTG Cuts at: 2083 3184 2083 Size: 1101 4942 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 2604 2817 Size: 54 1425 50 275 18 444 330 213 Cuts at: 2817 3279 3490 4379 6008 8 Size: 462 211 889 1629 43 Fragments arranged by size: 1629 1425 889 462 444 330 275 213 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2817 3490 1537 Size: 275 1005 673 4090 Fragments arranged by size: 4090 1005 673 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2027 2657 3973 1353 Size: 674 630 1316 3423 Fragments arranged by size: 3423 1316 674 630 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2734 3019 2734 Size: 285 5758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2367 3188 3218 3284 4106 4703 Size: 393 729 6 821 30 66 822 597 Cuts at: 4703 5490 5758 1239 Size: 787 268 1524 Fragments arranged by size: 1524 822 821 787 729 597 393 268 66 30 6 EcoNI CCTnn'n_nnAGG Cuts at: 2150 3179 3281 2150 Size: 1029 102 4912 Fragments arranged by size: 4912 1029 102 EcoO109I rG'GnC_Cy Cuts at: 2217 2218 2269 2351 2418 2842 2217 Size: 1 51 82 67 424 5418 Fragments arranged by size: 5418 424 82 67 51 1 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FspI TGC'GCA Cuts at: 2585 2627 4549 2585 Size: 42 1922 4079 Fragments arranged by size: 4079 1922 42 HaeII r_GCGC'y Cuts at: 1678 2769 5190 1678 Size: 1091 2421 2531 Fragments arranged by size: 2531 2421 1091 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 3044 3076 4684 Size: 32 135 32 929 32 511 32 1608 Cuts at: 4684 4716 4758 4790 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1608 929 511 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 3387 3633 669 Size: 988 281 247 1202 246 3079 Fragments arranged by size: 3079 1202 988 281 247 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 2765 2765 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5157 5341 1305 Size: 786 3066 184 2007 Fragments arranged by size: 3066 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2276 3281 10 Size: 54 1425 343 444 1005 2772 Fragments arranged by size: 2772 1425 1005 444 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2691 2795 3137 4001 4360 4519 518 Size: 648 1525 104 342 864 359 159 2042 Fragments arranged by size: 2042 1525 864 648 359 342 159 104 MspA1I CmG'CkG Cuts at: 1606 2264 2374 2443 2491 3241 3459 4136 5284 Size: 658 110 69 48 750 218 677 1148 Cuts at: 5284 5529 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 750 677 658 245 218 110 69 48 NaeI GCC'GGC Cuts at: 2168 2168 Size: 6043 NarI GG'CG_CC Cuts at: 2766 2766 Size: 6043 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5491 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NgoMIV G'CCGG_C Cuts at: 2166 2166 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 2863 1348 Size: 1515 4528 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6043 PfoI T'CCnGG_A Cuts at: 2754 3207 2754 Size: 453 5590 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2099 2131 4112 4144 5625 5657 2099 Size: 32 1981 32 1481 32 2485 Fragments arranged by size: 2485 1981 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2351 2351 Size: 6043 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PspOMI G'GGCC_C Cuts at: 2217 2417 2217 Size: 200 5843 PstI C_TGCA'G Cuts at: 830 2490 2841 3395 830 Size: 1660 351 554 3478 Fragments arranged by size: 3478 1660 554 351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2374 2491 2374 Size: 117 5926 SacI G_AGCT'C Cuts at: 2736 3021 2736 Size: 285 5758 SalI G'TCGA_C Cuts at: 1936 2183 3385 1936 Size: 247 1202 4594 Fragments arranged by size: 4594 1202 247 SanDI GG'GwC_CC Cuts at: 2351 2351 Size: 6043 SapI GCTCTTCn'nnn_ Cuts at: 2657 2657 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SexAI A'CCwGG_T Cuts at: 2718 2718 Size: 6043 SfcI C'TryA_G Cuts at: 826 1045 2486 2837 3391 4526 5207 5398 826 Size: 219 1441 351 554 1135 681 191 1471 Fragments arranged by size: 1471 1441 1135 681 554 351 219 191 SfoI GGC'GCC Cuts at: 2767 2767 Size: 6043 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 2215 3370 1872 Size: 343 1155 4545 Fragments arranged by size: 4545 1155 343 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3340 3834 493 Size: 2847 494 2702 Fragments arranged by size: 2847 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6043 StyI C'CwwG_G Cuts at: 513 1065 2223 3282 3482 513 Size: 552 1158 1059 200 3074 Fragments arranged by size: 3074 1158 1059 552 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2728 2801 3285 4070 4087 4240 4425 Size: 1255 1148 73 484 785 17 153 185 Cuts at: 4425 4870 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1148 785 484 445 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 4289 6001 Size: 274 80 33 51 1443 729 1583 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1712 1583 1443 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6003 Size: 32 6011 Tth111I GACn'n_nGTC Cuts at: 1316 2780 1316 Size: 1464 4579 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 2213 3368 1870 Size: 343 1155 4545 Fragments arranged by size: 4545 1155 343 XmnI GAAnn'nnTTC Cuts at: 3203 4172 3203 Size: 969 5074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI XcmI