Restriction map for
pFN21ASDA1142
(Linear) (Six-base) MAPSORT of: KIAA1142.seq Check: 6913 from: 1 to: 1314
KIAA1142.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AccI GT'mk_AC
Cuts at: 0 161 1028 1314
Size: 161 867 286
Fragments arranged by size:
867 286 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1165 1195 1261 1314
Size: 1165 30 66 53
Fragments arranged by size:
1165 66 53 30
AhdI GACnn_n'nnGTC
Cuts at: 0 1035 1314
Size: 1035 279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 1314
Size: 961 353
AlwNI CAG_nnn'CTG
Cuts at: 0 123 822 1314
Size: 123 699 492
Fragments arranged by size:
699 492 123
ApaI G_GGCC'C
Cuts at: 0 198 398 1314
Size: 198 200 916
Fragments arranged by size:
916 200 198
ApaLI G'TGCA_C
Cuts at: 0 61 1314
Size: 61 1253
AvaI C'yCGr_G
Cuts at: 0 190 293 299 567 1314
Size: 190 103 6 268 747
Fragments arranged by size:
747 268 190 103 6
BanI G'GyrC_C
Cuts at: 0 304 742 1314
Size: 304 438 572
Fragments arranged by size:
572 438 304
BanII G_rGCy'C
Cuts at: 0 198 300 347 398 532 713 998 1079
Size: 198 102 47 51 134 181 285 81
Cuts at: 1079 1314
Size: 235
Fragments arranged by size:
285 235 198 181 134 102 81 51
47
BbeI G_GCGC'C
Cuts at: 0 746 1314
Size: 746 568
BciVI GTATCCnnnnn_n'
Cuts at: 0 447 1314
Size: 447 867
BglI GCCn_nnn'nGGC
Cuts at: 0 1219 1314
Size: 1219 95
BglII A'GATC_T
Cuts at: 0 29 1314
Size: 29 1285
Bme1580I G_kGCm'C
Cuts at: 0 65 198 309 398 950 1289 1314
Size: 65 133 111 89 552 339 25
Fragments arranged by size:
552 339 133 111 89 65 25
BmgBI CAC'GTC
Cuts at: 0 219 1314
Size: 219 1095
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 1314
Size: 255 1059
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 763 1017 1314
Size: 763 254 297
Fragments arranged by size:
763 297 254
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 344 1314
Size: 338 6 970
Fragments arranged by size:
970 338 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 1199 1314
Size: 1199 115
BsaHI Gr'CG_yC
Cuts at: 0 743 845 1314
Size: 743 102 469
Fragments arranged by size:
743 469 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 478 508 1314
Size: 478 30 806
Fragments arranged by size:
806 478 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 258 261 264 267 796 1314
Size: 145 113 3 3 3 529 518
Fragments arranged by size:
529 518 145 113 3 3 3
BseYI C'CCAG_C
Cuts at: 0 310 324 347 1041 1214 1314
Size: 310 14 23 694 173 100
Fragments arranged by size:
694 310 173 100 23 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1138 1314
Size: 351 787 176
Fragments arranged by size:
787 351 176
BsiEI CG_ry'CG
Cuts at: 0 797 1314
Size: 797 517
BsiHKAI G_wGCw'C
Cuts at: 0 56 65 713 833 998 1314
Size: 56 9 648 120 165 316
Fragments arranged by size:
648 316 165 120 56 9
Bsp1286I G_dGCh'C
Cuts at: 0 56 65 198 300 309 347 398 532
Size: 56 9 133 102 9 38 51 134
Cuts at: 532 713 833 950 998 1079 1289 1314
Size: 181 120 117 48 81 210 25
Fragments arranged by size:
210 181 134 133 120 117 102 81
56 51 48 38 25 9 9
BspHI T'CATG_A
Cuts at: 0 213 647 1314
Size: 213 434 667
Fragments arranged by size:
667 434 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 608 1139 1160 1314
Size: 608 531 21 154
Fragments arranged by size:
608 531 154 21
BsrBI CCG'CTC
Cuts at: 0 410 494 1314
Size: 410 84 820
Fragments arranged by size:
820 410 84
BsrFI r'CCGG_y
Cuts at: 0 143 1314
Size: 143 1171
BsrGI T'GTAC_A
Cuts at: 0 683 1314
Size: 683 631
BssSI C'ACGA_G
Cuts at: 0 82 664 1314
Size: 82 582 650
Fragments arranged by size:
650 582 82
BstAPI GCAn_nnn'nTGC
Cuts at: 0 60 545 1314
Size: 60 485 769
Fragments arranged by size:
769 485 60
BstYI r'GATC_y
Cuts at: 0 29 1314
Size: 29 1285
BtgI C'CryG_G
Cuts at: 0 533 1223 1314
Size: 533 690 91
Fragments arranged by size:
690 533 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 534 1314
Size: 534 780
BtsI GCAGTG_nn'
Cuts at: 0 815 1314
Size: 815 499
DraIII CAC_nnn'GTG
Cuts at: 0 60 1161 1314
Size: 60 1101 153
Fragments arranged by size:
1101 153 60
EaeI y'GGCC_r
Cuts at: 0 251 581 794 1256 1314
Size: 251 330 213 462 58
Fragments arranged by size:
462 330 251 213 58
EagI C'GGCC_G
Cuts at: 0 794 1314
Size: 794 520
EarI CTCTTCn'nnn_
Cuts at: 0 4 634 1314
Size: 4 630 680
Fragments arranged by size:
680 630 4
EcoICRI GAG'CTC
Cuts at: 0 711 996 1314
Size: 711 285 318
Fragments arranged by size:
711 318 285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 344 1165 1195 1261 1314
Size: 338 6 821 30 66 53
Fragments arranged by size:
821 338 66 53 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 1156 1258 1314
Size: 127 1029 102 56
Fragments arranged by size:
1029 127 102 56
EcoO109I rG'GnC_Cy
Cuts at: 0 194 195 246 328 395 819 1314
Size: 194 1 51 82 67 424 495
Fragments arranged by size:
495 424 194 82 67 51 1
FspI TGC'GCA
Cuts at: 0 562 604 1314
Size: 562 42 710
Fragments arranged by size:
710 562 42
HaeII r_GCGC'y
Cuts at: 0 746 1314
Size: 746 568
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 1021 1053 1314
Size: 478 32 511 32 261
Fragments arranged by size:
511 478 261 32 32
HincII GTy'rAC
Cuts at: 0 162 1314
Size: 162 1152
KasI G'GCGC_C
Cuts at: 0 742 1314
Size: 742 572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1314
Size: 68 1246
MscI TGG'CCA
Cuts at: 0 253 1258 1314
Size: 253 1005 56
Fragments arranged by size:
1005 253 56
MslI CAynn'nnrTG
Cuts at: 0 668 772 1114 1314
Size: 668 104 342 200
Fragments arranged by size:
668 342 200 104
MspA1I CmG'CkG
Cuts at: 0 241 351 420 468 1218 1314
Size: 241 110 69 48 750 96
Fragments arranged by size:
750 241 110 96 69 48
NaeI GCC'GGC
Cuts at: 0 145 1314
Size: 145 1169
NarI GG'CG_CC
Cuts at: 0 743 1314
Size: 743 571
NgoMIV G'CCGG_C
Cuts at: 0 143 1314
Size: 143 1171
PasI CC'CwG_GG
Cuts at: 0 840 1314
Size: 840 474
PfoI T'CCnGG_A
Cuts at: 0 731 1184 1314
Size: 731 453 130
Fragments arranged by size:
731 453 130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1314
Size: 76 32 1206
Fragments arranged by size:
1206 76 32
PpuMI rG'GwC_Cy
Cuts at: 0 328 1314
Size: 328 986
PspOMI G'GGCC_C
Cuts at: 0 194 394 1314
Size: 194 200 920
Fragments arranged by size:
920 200 194
PstI C_TGCA'G
Cuts at: 0 467 818 1314
Size: 467 351 496
Fragments arranged by size:
496 467 351
PvuII CAG'CTG
Cuts at: 0 351 468 1314
Size: 351 117 846
Fragments arranged by size:
846 351 117
SacI G_AGCT'C
Cuts at: 0 713 998 1314
Size: 713 285 316
Fragments arranged by size:
713 316 285
SalI G'TCGA_C
Cuts at: 0 160 1314
Size: 160 1154
SanDI GG'GwC_CC
Cuts at: 0 328 1314
Size: 328 986
SapI GCTCTTCn'nnn_
Cuts at: 0 634 1314
Size: 634 680
SexAI A'CCwGG_T
Cuts at: 0 695 1314
Size: 695 619
SfcI C'TryA_G
Cuts at: 0 463 814 1314
Size: 463 351 500
Fragments arranged by size:
500 463 351
SfoI GGC'GCC
Cuts at: 0 744 1314
Size: 744 570
SmaI CCC'GGG
Cuts at: 0 192 1314
Size: 192 1122
StyI C'CwwG_G
Cuts at: 0 200 1259 1314
Size: 200 1059 55
Fragments arranged by size:
1059 200 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 705 778 1262 1314
Size: 705 73 484 52
Fragments arranged by size:
705 484 73 52
TatI w'GTAC_w
Cuts at: 0 683 1314
Size: 683 631
Tth111I GACn'n_nGTC
Cuts at: 0 757 1314
Size: 757 557
XmaI C'CCGG_G
Cuts at: 0 190 1314
Size: 190 1124
XmnI GAAnn'nnTTC
Cuts at: 0 1180 1314
Size: 1180 134
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BciVI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstYI BtgI BtgZI BtsI DraIII
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV PasI PfoI PpiI PpuMI PspOMI
PstI PvuII SacI SalI SanDI SapI SexAI SfcI
SfoI SmaI StyI TaqII TatI Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BclI BfrBI BlpI BmtI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EciI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SbfI ScaI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI ZraI